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The map label for this gene is thiO/thiG [H]

Identifier: 148259974

GI number: 148259974

Start: 1065825

End: 1066946

Strand: Reverse

Name: thiO/thiG [H]

Synonym: Acry_0964

Alternate gene names: 148259974

Gene position: 1066946-1065825 (Counterclockwise)

Preceding gene: 148259975

Following gene: 148259973

Centisome position: 31.48

GC content: 72.01

Gene sequence:

>1122_bases
ATGAAGGTCGCCATCATCGGCGGCGGCATCATCGGGCTCGCCCTGGGCTGGCGCCTCGCGCAGCGCGGCGCCGAGACCAC
GGTGTTCGAGCGCGATGCAATCGGCCGCGGCGCCAGCTACGCCGCGGCCGGGATGCTCGCCGCTGGCTGCGAGGCGGAGC
CGGGCGAACAGGCGCTCGGGGTGCTCAACCGCGAGTCGCTGGCGCTGTGGCCGGACTTCGCGGCGGAACTCGAGGCGGCC
ACCGGCATGGAGGTCGGGTTGCGGCGCGAGGGCACGATGCTGATCGCCCTCAACCGCGACGACGCGGCGAAGCTGCGCCA
CGACATGGAATTCCAGAAAAGCCAGGGCATCGAGCTCGAATGGCTGACGCCGGCCGAGGCGCGCGCGCGCGAGCCCTATC
TCGCGCCGCAACTGGCCGGGGCGGTGTTTTCGCCAGACGACATACAGGTCGACAACCGCGCGGCGACGGCGGCGCTGGCC
GAGGCGTTCCGCCGTGCGGGCGGGGTGCTGCGCGAGCGGATGCCCGTGCGGCGGATCGAGACCGATGGCAGCCGCGCCCG
GGGCGTGGTGACCGCCGAGGGGGTTCACGCGGCGGATGTGGTCGTGCTGGCGGCTGGCGCCTGGTCCGCCGGGTTCGATC
TGCCGGTGCCGCTGCCGCCGGTCCGCCCGATCAAGGGGCAGATGCTCTCGGTGCAGATGGACCCGGAAATCCCCCTGCTG
CGCCATGTGATCTGGGCGCCGCGGGCCTATCTGGTGCCACGGCGGAGTGGCCTGCTGGTCATCGGTGCGACGGTCGAGGA
ACGCGGGTTCGATGGCGCAATGACGGCCGGCGGGCTTTATTCGCTGCTCGACGGCGCCTGGCGCGCCTTGCCGGGGATCG
AGGAGCTCGCCGTGGTGGAAACCTGGGTCGGTTTCCGGCCCGGATCGCGCGATGACGCCCCTGTGCTCGGTCCCAGCGCC
CTGCCCGGCCTGATGTTCTGCACCGGGCATCACCGCAACGGCATTCTGCTCACCCCGGTCACCGCCGCGGCGATGGCGGC
GACGATCCTGGAGCAGAAGCTTGATCCGCGCATCGCCCCGTTCGGGATCGGGCGCTTCGCGCGGGTGAGGAGTGCTGCAT
GA

Upstream 100 bases:

>100_bases
GACGAGGTGGCGCGCGAGGAAGGGCTCGCGCAGAAGGCGGCGGAGTTCCGCGATGGCGGCAGCCGCATCTATGTCGAGGA
TCAGGCGGCGGAGTGAAGCC

Downstream 100 bases:

>100_bases
ATCGCGAGGAAATCCGGGTGAACGGCGCGTTCGAGCCGATTGCGGCGAGCATTGCCGCATTGCTGGAGGCGAAGGGGCTG
CATGAAACCAAGGGGCTGGC

Product: glycine oxidase ThiO

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKVAIIGGGIIGLALGWRLAQRGAETTVFERDAIGRGASYAAAGMLAAGCEAEPGEQALGVLNRESLALWPDFAAELEAA
TGMEVGLRREGTMLIALNRDDAAKLRHDMEFQKSQGIELEWLTPAEARAREPYLAPQLAGAVFSPDDIQVDNRAATAALA
EAFRRAGGVLRERMPVRRIETDGSRARGVVTAEGVHAADVVVLAAGAWSAGFDLPVPLPPVRPIKGQMLSVQMDPEIPLL
RHVIWAPRAYLVPRRSGLLVIGATVEERGFDGAMTAGGLYSLLDGAWRALPGIEELAVVETWVGFRPGSRDDAPVLGPSA
LPGLMFCTGHHRNGILLTPVTAAAMAATILEQKLDPRIAPFGIGRFARVRSAA

Sequences:

>Translated_373_residues
MKVAIIGGGIIGLALGWRLAQRGAETTVFERDAIGRGASYAAAGMLAAGCEAEPGEQALGVLNRESLALWPDFAAELEAA
TGMEVGLRREGTMLIALNRDDAAKLRHDMEFQKSQGIELEWLTPAEARAREPYLAPQLAGAVFSPDDIQVDNRAATAALA
EAFRRAGGVLRERMPVRRIETDGSRARGVVTAEGVHAADVVVLAAGAWSAGFDLPVPLPPVRPIKGQMLSVQMDPEIPLL
RHVIWAPRAYLVPRRSGLLVIGATVEERGFDGAMTAGGLYSLLDGAWRALPGIEELAVVETWVGFRPGSRDDAPVLGPSA
LPGLMFCTGHHRNGILLTPVTAAAMAATILEQKLDPRIAPFGIGRFARVRSAA
>Mature_373_residues
MKVAIIGGGIIGLALGWRLAQRGAETTVFERDAIGRGASYAAAGMLAAGCEAEPGEQALGVLNRESLALWPDFAAELEAA
TGMEVGLRREGTMLIALNRDDAAKLRHDMEFQKSQGIELEWLTPAEARAREPYLAPQLAGAVFSPDDIQVDNRAATAALA
EAFRRAGGVLRERMPVRRIETDGSRARGVVTAEGVHAADVVVLAAGAWSAGFDLPVPLPPVRPIKGQMLSVQMDPEIPLL
RHVIWAPRAYLVPRRSGLLVIGATVEERGFDGAMTAGGLYSLLDGAWRALPGIEELAVVETWVGFRPGSRDDAPVLGPSA
LPGLMFCTGHHRNGILLTPVTAAAMAATILEQKLDPRIAPFGIGRFARVRSAA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Homo sapiens, GI197927446, Length=390, Percent_Identity=24.8717948717949, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21361378, Length=390, Percent_Identity=24.8717948717949, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI24797151, Length=268, Percent_Identity=25.7462686567164, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787438, Length=381, Percent_Identity=23.3595800524934, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20130091, Length=396, Percent_Identity=24.7474747474747, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 39610; Mature: 39610

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAIIGGGIIGLALGWRLAQRGAETTVFERDAIGRGASYAAAGMLAAGCEAEPGEQALG
CEEEEECCHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCHHHHHHHHCCCCCCCCHHHHH
VLNRESLALWPDFAAELEAATGMEVGLRREGTMLIALNRDDAAKLRHDMEFQKSQGIELE
HHCCCCEEECCCHHHHHHHHCCCEECEECCCEEEEEECCCCHHHHHHHHHHHHHCCCEEE
WLTPAEARAREPYLAPQLAGAVFSPDDIQVDNRAATAALAEAFRRAGGVLRERMPVRRIE
EECCHHHHCCCCCCCHHHHCCEECCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEC
TDGSRARGVVTAEGVHAADVVVLAAGAWSAGFDLPVPLPPVRPIKGQMLSVQMDPEIPLL
CCCCHHCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHH
RHVIWAPRAYLVPRRSGLLVIGATVEERGFDGAMTAGGLYSLLDGAWRALPGIEELAVVE
HHHHHCCCEEEEECCCCEEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
TWVGFRPGSRDDAPVLGPSALPGLMFCTGHHRNGILLTPVTAAAMAATILEQKLDPRIAP
HHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCHHHHHHHHHHHHHHCCCCCCC
FGIGRFARVRSAA
CCCCHHHHHHCCC
>Mature Secondary Structure
MKVAIIGGGIIGLALGWRLAQRGAETTVFERDAIGRGASYAAAGMLAAGCEAEPGEQALG
CEEEEECCHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCHHHHHHHHCCCCCCCCHHHHH
VLNRESLALWPDFAAELEAATGMEVGLRREGTMLIALNRDDAAKLRHDMEFQKSQGIELE
HHCCCCEEECCCHHHHHHHHCCCEECEECCCEEEEEECCCCHHHHHHHHHHHHHCCCEEE
WLTPAEARAREPYLAPQLAGAVFSPDDIQVDNRAATAALAEAFRRAGGVLRERMPVRRIE
EECCHHHHCCCCCCCHHHHCCEECCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEC
TDGSRARGVVTAEGVHAADVVVLAAGAWSAGFDLPVPLPPVRPIKGQMLSVQMDPEIPLL
CCCCHHCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHH
RHVIWAPRAYLVPRRSGLLVIGATVEERGFDGAMTAGGLYSLLDGAWRALPGIEELAVVE
HHHHHCCCEEEEECCCCEEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
TWVGFRPGSRDDAPVLGPSALPGLMFCTGHHRNGILLTPVTAAAMAATILEQKLDPRIAP
HHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCHHHHHHHHHHHHHHCCCCCCC
FGIGRFARVRSAA
CCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA