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The map label for this gene is bigR [H]

Identifier: 148259971

GI number: 148259971

Start: 1064295

End: 1064702

Strand: Reverse

Name: bigR [H]

Synonym: Acry_0961

Alternate gene names: 148259971

Gene position: 1064702-1064295 (Counterclockwise)

Preceding gene: 148259972

Following gene: 148259957

Centisome position: 31.41

GC content: 64.95

Gene sequence:

>408_bases
ATGGGTCAGAGCGCCCCCTTGACAGCTGAGGAAGATATTCGGGCCGACATGGCGAAGTCCATCCTGCCGTCGGATGACAG
CGAGATCGAACTCGACGCGATGATGCGAAACGCAACACTTGCGTCGCAGTTCCTGCGCTGCATTGCCAATCCGCACCGGC
TGATGGTGCTTTGCCATCTCACGCATGGCGAAGCGTCGGTCGGCCAGCTTGAGCGCGAACTGGGCATCCGCCAGGCGCAT
CTGTCGCAGCAGCTGGCGCGGCTGCGGCAGGACGGAATGGTAACGACGCGCCGCGATTCCCGGACGATCTACTACTCGCT
CGGCAGCGAGGCGGCGCGGGAGGTTGTCGCGCTGATCTACCGGCTGTTCTGCACCGCGGAGACGGGTAGCGCGGGCGGCT
GCGGCTGA

Upstream 100 bases:

>100_bases
GGCCTCGTGGCCGGAAACCACGGCTCACGTATTGCCAAAGGCTGATCGATCAGATCATAGTCGCTGCCTATAAAGCGAGC
TTGGACACGGGGAACACAAA

Downstream 100 bases:

>100_bases
CGCGCGGCGTCGAGCCCCCGCACGACCCAGATTCCGGCCAGCATCGCGCCCACGAACGGCGCGATGACGAGCGGATCCAG
TGCCAGCGCCGTGATGGCCG

Product: regulatory protein, ArsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 135; Mature: 134

Protein sequence:

>135_residues
MGQSAPLTAEEDIRADMAKSILPSDDSEIELDAMMRNATLASQFLRCIANPHRLMVLCHLTHGEASVGQLERELGIRQAH
LSQQLARLRQDGMVTTRRDSRTIYYSLGSEAAREVVALIYRLFCTAETGSAGGCG

Sequences:

>Translated_135_residues
MGQSAPLTAEEDIRADMAKSILPSDDSEIELDAMMRNATLASQFLRCIANPHRLMVLCHLTHGEASVGQLERELGIRQAH
LSQQLARLRQDGMVTTRRDSRTIYYSLGSEAAREVVALIYRLFCTAETGSAGGCG
>Mature_134_residues
GQSAPLTAEEDIRADMAKSILPSDDSEIELDAMMRNATLASQFLRCIANPHRLMVLCHLTHGEASVGQLERELGIRQAHL
SQQLARLRQDGMVTTRRDSRTIYYSLGSEAAREVVALIYRLFCTAETGSAGGCG

Specific function: Represses an operon that comprises at least itself and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=96, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 14795; Mature: 14664

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQSAPLTAEEDIRADMAKSILPSDDSEIELDAMMRNATLASQFLRCIANPHRLMVLCHL
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
THGEASVGQLERELGIRQAHLSQQLARLRQDGMVTTRRDSRTIYYSLGSEAAREVVALIY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCHHHHHHHHHHHH
RLFCTAETGSAGGCG
HHHHCCCCCCCCCCC
>Mature Secondary Structure 
GQSAPLTAEEDIRADMAKSILPSDDSEIELDAMMRNATLASQFLRCIANPHRLMVLCHL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
THGEASVGQLERELGIRQAHLSQQLARLRQDGMVTTRRDSRTIYYSLGSEAAREVVALIY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCHHHHHHHHHHHH
RLFCTAETGSAGGCG
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]