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The map label for this gene is bcsZ [H]

Identifier: 148259586

GI number: 148259586

Start: 641181

End: 642311

Strand: Reverse

Name: bcsZ [H]

Synonym: Acry_0570

Alternate gene names: 148259586

Gene position: 642311-641181 (Counterclockwise)

Preceding gene: 148259587

Following gene: 148259585

Centisome position: 18.95

GC content: 68.44

Gene sequence:

>1131_bases
ATGACCGACATGGCGAGACGCAATTTTCTCGGTCTTCTCGGCGCGGCCGCGCTCACGGGCGGCCCGGTGATGATGGCGCG
GGAGCTGTGGCAATCGTCCTGGCGCGGCTACCGGCACGGATTCATCGACGGCCAGGGGCGGGTCATCGACTATTCCGCCA
ACAAGGGATTCAGCACTTCGGAGGGGCAGTCCTATGGCATGTTCCTCAGTCTCGTCGCCGGCGACCGCGCGACGTTCCGC
CGCATCCTGAACTGGACGAACACGAACATGGCGGGCGGGCGCCTCGGGGAGGTGCTCGCGGCGTGGAAATGGGGGCTGCA
CGGCGGCAAATGGGGGGTGATCGGCGCCAACTCGGCGGCGGACGCGGATGCGTGGATGGCCTATTCGCTGCTCGAGGCCG
CCCGGATCTGGAAGGATCACAATCTCGGCGCCGAGGGGCACAAGCTGGCGACGCGCATCGCCGATGACGAGAGCGTGGCG
ATCAACGGATTTGGCCGGGTCCTGATACCCGGCGCCTCCGATTTTCCGGACACGCCGCCCGTCATCGTCGATCCGAGCTA
TACGCCGCTGTTTCTGGCCCGCGGCATCGCGCGCGCGACCAACCTGCCGAAATGGCAGGCGATCGCGGCCACGCTGCCAC
GGCTGATGACGACGATCTGCCGCAACGGATTCGCCCCCGACTGGGCCTGGGCGCCGCAAGCCCCCGCCTCGCCGCCGGCG
GGCCTGCCGGAGACCGGCACCGGATCGTTCGATGCGATCCGGTGCTATCTCTGGGCGGGCCTGACCGCGCCGGAAACGGA
GGGGAGCGCGACCGTTCTCGCCTCGCTGAAGGGCATGGCCCGATACTTGGCCACCCACCGCGCGCCGCCGCAGAGCGTCG
ATCTCGCGAGCCAGGCGACGCACGGGACGGGCGGGATCGGGTTTTCCGCGGCGCTGCTGCCCTACCTCGCGGCACTCGGG
CGCCACCGGCTGCTCCATCAGCAGCTCGGCCGCGTGCTGGCCCAGCGGGAGACCAGCGGCCTGTTCGGCCAGCCCGCCGA
CTATTACTCGGAAAACCTGATCCTGTTCGGACTTGGCGGACTTTCGGGAAGCATCCGCTTCGACAAGCAAGGAGGTCTGA
TCACGTCATGA

Upstream 100 bases:

>100_bases
TCCCTCGATGTCGATCTGTCCGCCGGCGCGACGACGAACCTTCCGGGTCCGTATCACCCGTATGTCGCCCTGAATCTCAG
CGTGCCGTTCCGGTAATCGC

Downstream 100 bases:

>100_bases
TGCGCCATCTCCCCGCGTTGACCAATGGCCTCACCTTCATCGTCGCCGCAGCCGTGGGCTTCGTGCTCATCGCCGCCACG
CTGCTGCCCACGACGGGGCC

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTDMARRNFLGLLGAAALTGGPVMMARELWQSSWRGYRHGFIDGQGRVIDYSANKGFSTSEGQSYGMFLSLVAGDRATFR
RILNWTNTNMAGGRLGEVLAAWKWGLHGGKWGVIGANSAADADAWMAYSLLEAARIWKDHNLGAEGHKLATRIADDESVA
INGFGRVLIPGASDFPDTPPVIVDPSYTPLFLARGIARATNLPKWQAIAATLPRLMTTICRNGFAPDWAWAPQAPASPPA
GLPETGTGSFDAIRCYLWAGLTAPETEGSATVLASLKGMARYLATHRAPPQSVDLASQATHGTGGIGFSAALLPYLAALG
RHRLLHQQLGRVLAQRETSGLFGQPADYYSENLILFGLGGLSGSIRFDKQGGLITS

Sequences:

>Translated_376_residues
MTDMARRNFLGLLGAAALTGGPVMMARELWQSSWRGYRHGFIDGQGRVIDYSANKGFSTSEGQSYGMFLSLVAGDRATFR
RILNWTNTNMAGGRLGEVLAAWKWGLHGGKWGVIGANSAADADAWMAYSLLEAARIWKDHNLGAEGHKLATRIADDESVA
INGFGRVLIPGASDFPDTPPVIVDPSYTPLFLARGIARATNLPKWQAIAATLPRLMTTICRNGFAPDWAWAPQAPASPPA
GLPETGTGSFDAIRCYLWAGLTAPETEGSATVLASLKGMARYLATHRAPPQSVDLASQATHGTGGIGFSAALLPYLAALG
RHRLLHQQLGRVLAQRETSGLFGQPADYYSENLILFGLGGLSGSIRFDKQGGLITS
>Mature_375_residues
TDMARRNFLGLLGAAALTGGPVMMARELWQSSWRGYRHGFIDGQGRVIDYSANKGFSTSEGQSYGMFLSLVAGDRATFRR
ILNWTNTNMAGGRLGEVLAAWKWGLHGGKWGVIGANSAADADAWMAYSLLEAARIWKDHNLGAEGHKLATRIADDESVAI
NGFGRVLIPGASDFPDTPPVIVDPSYTPLFLARGIARATNLPKWQAIAATLPRLMTTICRNGFAPDWAWAPQAPASPPAG
LPETGTGSFDAIRCYLWAGLTAPETEGSATVLASLKGMARYLATHRAPPQSVDLASQATHGTGGIGFSAALLPYLAALGR
HRLLHQQLGRVLAQRETSGLFGQPADYYSENLILFGLGGLSGSIRFDKQGGLITS

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=350, Percent_Identity=38, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 40130; Mature: 39999

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDMARRNFLGLLGAAALTGGPVMMARELWQSSWRGYRHGFIDGQGRVIDYSANKGFSTS
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCCCCCCEEEEECCCCCCCCC
EGQSYGMFLSLVAGDRATFRRILNWTNTNMAGGRLGEVLAAWKWGLHGGKWGVIGANSAA
CCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCC
DADAWMAYSLLEAARIWKDHNLGAEGHKLATRIADDESVAINGFGRVLIPGASDFPDTPP
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCCC
VIVDPSYTPLFLARGIARATNLPKWQAIAATLPRLMTTICRNGFAPDWAWAPQAPASPPA
EEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GLPETGTGSFDAIRCYLWAGLTAPETEGSATVLASLKGMARYLATHRAPPQSVDLASQAT
CCCCCCCCCHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCC
HGTGGIGFSAALLPYLAALGRHRLLHQQLGRVLAQRETSGLFGQPADYYSENLILFGLGG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEEEEECC
LSGSIRFDKQGGLITS
CCCCEEECCCCCCCCC
>Mature Secondary Structure 
TDMARRNFLGLLGAAALTGGPVMMARELWQSSWRGYRHGFIDGQGRVIDYSANKGFSTS
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCCCCCCEEEEECCCCCCCCC
EGQSYGMFLSLVAGDRATFRRILNWTNTNMAGGRLGEVLAAWKWGLHGGKWGVIGANSAA
CCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCC
DADAWMAYSLLEAARIWKDHNLGAEGHKLATRIADDESVAINGFGRVLIPGASDFPDTPP
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCCC
VIVDPSYTPLFLARGIARATNLPKWQAIAATLPRLMTTICRNGFAPDWAWAPQAPASPPA
EEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GLPETGTGSFDAIRCYLWAGLTAPETEGSATVLASLKGMARYLATHRAPPQSVDLASQAT
CCCCCCCCCHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCC
HGTGGIGFSAALLPYLAALGRHRLLHQQLGRVLAQRETSGLFGQPADYYSENLILFGLGG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEEEEECC
LSGSIRFDKQGGLITS
CCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]