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The map label for this gene is ispDF [H]

Identifier: 148259567

GI number: 148259567

Start: 615354

End: 616049

Strand: Reverse

Name: ispDF [H]

Synonym: Acry_0551

Alternate gene names: 148259567

Gene position: 616049-615354 (Counterclockwise)

Preceding gene: 148259569

Following gene: 148259564

Centisome position: 18.18

GC content: 70.55

Gene sequence:

>696_bases
ATGACCGTTGCACTGATCGTCGCCGCCGGCCGGGGCCATCGCCTCGGCGGGCCCATTCCAAAACAGTATCGCCTGCTCGA
CGGCGTGCCGATCCTTCGCCGCACGGCGGAGGCACTGCGCCGGCATGCCTCGATGCGCGCCGTGTTCGTGGTGATCAATC
CGGCGGACCGCGCGCTGTATGACGAGGCGATGTCGGGGCTTGGCCTGCCGGAACCGGTCGCTGGCGGCGCCTCGCGGCAG
GAATCGGTGCGCAACGGGCTTGAGGCGATCGCCAGCGTCGCACCGGAGAGGGTGCTGATCCATGACGGGGTGCGGCCATT
CGTGGCGCCGGACGTGATCGGCGCGGTGATCGGCGCGCTCGACCGCGTGCCGGGCGCCGTGGTGGGCGTGCCGGTGACCG
ATACGCTGAAACGCTGCAGCGGCGCGATCGTGACCGGCACGGTGGACCGCGCCGATCTGTGGCGGGCACAGACGCCGCAG
GGGTTCCGCTATGACGCAATTCTCGGCGCGCACCGACTGGCGCAGACGGCGCATCGGGGGCGGGATTTCACCGATGACTG
CGCGGTCGCCGAACTCGCGGGGCTGGCGATCGAGATGGTTCCGGGCAGCGAGGAGAATTTCAAGATCACCACGGAGGCCG
ACCTGCTGCGGGCCGAGGCGCTGCTTCGCGAACGGGCGCGCGCACTCGCGGGATAG

Upstream 100 bases:

>100_bases
TAATCCGATCACAAGCCCGCGCCAGGCGGTTTGCCTTTGCCGGCCGGTTCTGCTTTCAGGGAGCCGAAGCGCGGCGCCGC
CGCGCCGAGCCGGGGACCGC

Downstream 100 bases:

>100_bases
GGCCGCCCTGCCCGGCCCCGGCGCGACGCCTTAACCGATTCCGGCCGCCGCGATCAGCACGACTCCAAGCCCGACCAGCA
CGAGGCCGGTGATCTCGCCG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTVALIVAAGRGHRLGGPIPKQYRLLDGVPILRRTAEALRRHASMRAVFVVINPADRALYDEAMSGLGLPEPVAGGASRQ
ESVRNGLEAIASVAPERVLIHDGVRPFVAPDVIGAVIGALDRVPGAVVGVPVTDTLKRCSGAIVTGTVDRADLWRAQTPQ
GFRYDAILGAHRLAQTAHRGRDFTDDCAVAELAGLAIEMVPGSEENFKITTEADLLRAEALLRERARALAG

Sequences:

>Translated_231_residues
MTVALIVAAGRGHRLGGPIPKQYRLLDGVPILRRTAEALRRHASMRAVFVVINPADRALYDEAMSGLGLPEPVAGGASRQ
ESVRNGLEAIASVAPERVLIHDGVRPFVAPDVIGAVIGALDRVPGAVVGVPVTDTLKRCSGAIVTGTVDRADLWRAQTPQ
GFRYDAILGAHRLAQTAHRGRDFTDDCAVAELAGLAIEMVPGSEENFKITTEADLLRAEALLRERARALAG
>Mature_230_residues
TVALIVAAGRGHRLGGPIPKQYRLLDGVPILRRTAEALRRHASMRAVFVVINPADRALYDEAMSGLGLPEPVAGGASRQE
SVRNGLEAIASVAPERVLIHDGVRPFVAPDVIGAVIGALDRVPGAVVGVPVTDTLKRCSGAIVTGTVDRADLWRAQTPQG
FRYDAILGAHRLAQTAHRGRDFTDDCAVAELAGLAIEMVPGSEENFKITTEADLLRAEALLRERARALAG

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=234, Percent_Identity=28.2051282051282, Blast_Score=84, Evalue=9e-17,
Organism=Escherichia coli, GI1789104, Length=223, Percent_Identity=30.9417040358744, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 24505; Mature: 24373

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVALIVAAGRGHRLGGPIPKQYRLLDGVPILRRTAEALRRHASMRAVFVVINPADRALY
CEEEEEEECCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHH
DEAMSGLGLPEPVAGGASRQESVRNGLEAIASVAPERVLIHDGVRPFVAPDVIGAVIGAL
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
DRVPGAVVGVPVTDTLKRCSGAIVTGTVDRADLWRAQTPQGFRYDAILGAHRLAQTAHRG
HHCCCCEEECCHHHHHHHHCCCEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
RDFTDDCAVAELAGLAIEMVPGSEENFKITTEADLLRAEALLRERARALAG
CCCCHHHHHHHHHCCEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVALIVAAGRGHRLGGPIPKQYRLLDGVPILRRTAEALRRHASMRAVFVVINPADRALY
EEEEEEECCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHH
DEAMSGLGLPEPVAGGASRQESVRNGLEAIASVAPERVLIHDGVRPFVAPDVIGAVIGAL
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
DRVPGAVVGVPVTDTLKRCSGAIVTGTVDRADLWRAQTPQGFRYDAILGAHRLAQTAHRG
HHCCCCEEECCHHHHHHHHCCCEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
RDFTDDCAVAELAGLAIEMVPGSEENFKITTEADLLRAEALLRERARALAG
CCCCHHHHHHHHHCCEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA