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The map label for this gene is paaK [H]

Identifier: 148259493

GI number: 148259493

Start: 530343

End: 531626

Strand: Reverse

Name: paaK [H]

Synonym: Acry_0477

Alternate gene names: 148259493

Gene position: 531626-530343 (Counterclockwise)

Preceding gene: 148259494

Following gene: 148259485

Centisome position: 15.69

GC content: 68.54

Gene sequence:

>1284_bases
ATGGAGCGGGACGTCGAATTCGCCTCGCGTGACGAGGTTGTCGCGCTGCAGACGAGGCGGCTGAGGGAGGCGCTGCGCCA
TGCCTATGAGCGCGTGCCATTCTATCGCTCGGCGTTCGATGCGGCGGGGGTGCGGCCGGAGGATTTGCAGCGGCCGGAGG
ATCTGGCGCGGTTTCCGTTCACGGTGAAGGACGATCTGCGGCAGACCTATCCGTTCGGCATGTTCGCGGTGCCGCGCGGC
GAGGTGTTGCGGCTGCACGCCTCGTCCGGGACAACGGGACGGCCGATCGTGGTGGGCTATACGCGGGCGGATCTCGATGT
CTGGGCCGGGCTGGTCGCGCGGTCGATCCGGGCGGCGGGCGGACGGGCGGGCATGATGGTGCATGTCGCCTACGGCTATG
GGCTGTTCACCGGCGGGCTCGGCGCGCATGCGGGCGCGGAGCGGCTCGGCTGCACGGTGGTGCCGGCCTCGGGCGGGATG
ACCGAGCGGCAGGTGCAGTTGATCGCGGATTTCGGGCCCGAGATCATCATGGTGACGCCGAGCTACGCGCTCGCGATCCT
CGACGAGTTCCGCCGCCAGGGGCGCGATCCGCGGGCGACAGCACTGCAATACGGAATTTTCGGCGCGGAACCCTGGACCA
ATGCGATGCGCGCCGAGATCGAATCGGCGTTCGGCATCGAGGCGACCGATATCTACGGGCTTTCGGAGCTGATGGGGCCG
GGGGTGGCGCAGGAATGCGTCGAGACCAAGGACGGGCTGCATCTCTGGGAGGACCATTTCTACCCCGAGATCATCGATCC
GGCGACCGGCGCGGTGCTGCCGGATGGGAAGACCGGCGAGCTGGTGCTGACTGCGCTGAGCCACGAGGCGATGCCGATCA
TCCGCTACCGGACACGCGACCTCACGCGGCTGCTGCCTGGTACGGCGCGGCCGGGGATGCGGCGGATGGAGAAGATCGGC
GGGCGGGCGGACGACATGATCATTCTCCGCGGCGTGAACCTGTTTCCGAGCCAGATCGAGGAATTGCTGCTCGCCAACGA
ATGGGGCAGCGGGCATTTCGTGATCGAGCTGACGCGGCCGCAGCGGCTGGACGAGATGACGGTGCGGGCCGAGTGCCGCC
CCGAGCACTGGGACGGCGCCGGGCTCGCCGGCGAGGCCGCCGCCTTTGCGCATCACATCAAGGCGACGATCGGCGTCACA
GCGCATGTGGTCATCGAACCGCCTGGGACTCTCGAGCGGTCGGTCGGCAAGGCAAAGCGGGTGCGCGACCTGCGGCCGAA
ATGA

Upstream 100 bases:

>100_bases
GATGTTTCGGTCAGCAACGATGACGGAATCGTCGCGGCGTTCCGCGGACAGTCGCGCACGATCGGCGGCTCGTGGCTCGA
TGACAACGGAAGGTAGCGGG

Downstream 100 bases:

>100_bases
TCAGGCGACGAGGTTGGCGGGGCTCCGCCCTGCCAGTACCTCCTCGATCCCGTCGAGCGCCAGCATGCCCATCGCAGTGC
GGGTCTCGCGCGTGGCGCTG

Product: phenylacetate-CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MERDVEFASRDEVVALQTRRLREALRHAYERVPFYRSAFDAAGVRPEDLQRPEDLARFPFTVKDDLRQTYPFGMFAVPRG
EVLRLHASSGTTGRPIVVGYTRADLDVWAGLVARSIRAAGGRAGMMVHVAYGYGLFTGGLGAHAGAERLGCTVVPASGGM
TERQVQLIADFGPEIIMVTPSYALAILDEFRRQGRDPRATALQYGIFGAEPWTNAMRAEIESAFGIEATDIYGLSELMGP
GVAQECVETKDGLHLWEDHFYPEIIDPATGAVLPDGKTGELVLTALSHEAMPIIRYRTRDLTRLLPGTARPGMRRMEKIG
GRADDMIILRGVNLFPSQIEELLLANEWGSGHFVIELTRPQRLDEMTVRAECRPEHWDGAGLAGEAAAFAHHIKATIGVT
AHVVIEPPGTLERSVGKAKRVRDLRPK

Sequences:

>Translated_427_residues
MERDVEFASRDEVVALQTRRLREALRHAYERVPFYRSAFDAAGVRPEDLQRPEDLARFPFTVKDDLRQTYPFGMFAVPRG
EVLRLHASSGTTGRPIVVGYTRADLDVWAGLVARSIRAAGGRAGMMVHVAYGYGLFTGGLGAHAGAERLGCTVVPASGGM
TERQVQLIADFGPEIIMVTPSYALAILDEFRRQGRDPRATALQYGIFGAEPWTNAMRAEIESAFGIEATDIYGLSELMGP
GVAQECVETKDGLHLWEDHFYPEIIDPATGAVLPDGKTGELVLTALSHEAMPIIRYRTRDLTRLLPGTARPGMRRMEKIG
GRADDMIILRGVNLFPSQIEELLLANEWGSGHFVIELTRPQRLDEMTVRAECRPEHWDGAGLAGEAAAFAHHIKATIGVT
AHVVIEPPGTLERSVGKAKRVRDLRPK
>Mature_427_residues
MERDVEFASRDEVVALQTRRLREALRHAYERVPFYRSAFDAAGVRPEDLQRPEDLARFPFTVKDDLRQTYPFGMFAVPRG
EVLRLHASSGTTGRPIVVGYTRADLDVWAGLVARSIRAAGGRAGMMVHVAYGYGLFTGGLGAHAGAERLGCTVVPASGGM
TERQVQLIADFGPEIIMVTPSYALAILDEFRRQGRDPRATALQYGIFGAEPWTNAMRAEIESAFGIEATDIYGLSELMGP
GVAQECVETKDGLHLWEDHFYPEIIDPATGAVLPDGKTGELVLTALSHEAMPIIRYRTRDLTRLLPGTARPGMRRMEKIG
GRADDMIILRGVNLFPSQIEELLLANEWGSGHFVIELTRPQRLDEMTVRAECRPEHWDGAGLAGEAAAFAHHIKATIGVT
AHVVIEPPGTLERSVGKAKRVRDLRPK

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=427, Percent_Identity=58.07962529274, Blast_Score=495, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 47006; Mature: 47006

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERDVEFASRDEVVALQTRRLREALRHAYERVPFYRSAFDAAGVRPEDLQRPEDLARFPF
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHCCCCHHHHHCCC
TVKDDLRQTYPFGMFAVPRGEVLRLHASSGTTGRPIVVGYTRADLDVWAGLVARSIRAAG
CHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
GRAGMMVHVAYGYGLFTGGLGAHAGAERLGCTVVPASGGMTERQVQLIADFGPEIIMVTP
CCCCEEEEEEECCHHHCCCCCCCCCHHHCCEEEEECCCCCCHHHHHHHCCCCCCEEEECC
SYALAILDEFRRQGRDPRATALQYGIFGAEPWTNAMRAEIESAFGIEATDIYGLSELMGP
HHHHHHHHHHHHCCCCCCHHHHEECCCCCCCHHHHHHHHHHHHCCCCHHHHCCHHHHHCC
GVAQECVETKDGLHLWEDHFYPEIIDPATGAVLPDGKTGELVLTALSHEAMPIIRYRTRD
CHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHH
LTRLLPGTARPGMRRMEKIGGRADDMIILRGVNLFPSQIEELLLANEWGSGHFVIELTRP
HHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCC
QRLDEMTVRAECRPEHWDGAGLAGEAAAFAHHIKATIGVTAHVVIEPPGTLERSVGKAKR
CCCHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHEECEEEEEEECCCCHHHHHHHHHHH
VRDLRPK
HHCCCCC
>Mature Secondary Structure
MERDVEFASRDEVVALQTRRLREALRHAYERVPFYRSAFDAAGVRPEDLQRPEDLARFPF
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHCCCCHHHHHCCC
TVKDDLRQTYPFGMFAVPRGEVLRLHASSGTTGRPIVVGYTRADLDVWAGLVARSIRAAG
CHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
GRAGMMVHVAYGYGLFTGGLGAHAGAERLGCTVVPASGGMTERQVQLIADFGPEIIMVTP
CCCCEEEEEEECCHHHCCCCCCCCCHHHCCEEEEECCCCCCHHHHHHHCCCCCCEEEECC
SYALAILDEFRRQGRDPRATALQYGIFGAEPWTNAMRAEIESAFGIEATDIYGLSELMGP
HHHHHHHHHHHHCCCCCCHHHHEECCCCCCCHHHHHHHHHHHHCCCCHHHHCCHHHHHCC
GVAQECVETKDGLHLWEDHFYPEIIDPATGAVLPDGKTGELVLTALSHEAMPIIRYRTRD
CHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHH
LTRLLPGTARPGMRRMEKIGGRADDMIILRGVNLFPSQIEELLLANEWGSGHFVIELTRP
HHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCC
QRLDEMTVRAECRPEHWDGAGLAGEAAAFAHHIKATIGVTAHVVIEPPGTLERSVGKAKR
CCCHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHEECEEEEEEECCCCHHHHHHHHHHH
VRDLRPK
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]