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The map label for this gene is ubiH [H]

Identifier: 148259393

GI number: 148259393

Start: 427811

End: 429001

Strand: Reverse

Name: ubiH [H]

Synonym: Acry_0376

Alternate gene names: 148259393

Gene position: 429001-427811 (Counterclockwise)

Preceding gene: 148259404

Following gene: 148259390

Centisome position: 12.66

GC content: 71.45

Gene sequence:

>1191_bases
ATGACGAACGACACAGCGGATGTCGCAATCATCGGGGCGGGGCCGGTCGGGGCGGCGCTTGCACTGCGCCTCGCTGCCGG
CGGGCAGCGGGTCGTGCTCGTCGACCGGGCGGCGCTGCCGCCGATGGAACATCCGGATTTCGATGGCCGCGCCTATGCCA
TCGCCTACGGCTCCCGCGACCTGCTCGACCGTGCCGGGCTATGGGACCGCTTGCCCTTTCCGCCCTGCCCGATCGAACGC
ATCCGCGTCTCGGACGGCAAGCCGGGCCGCCCGCCCTCGCCGCTGTTTCTCGATTTCGATCATCGCGATGTCGGCGACGA
GCCGTTCGGCTGGATTGTCGAAGCCCGCTCGCTGCGCATGGCGATCAACCGCGCCCTCGCCGACTCGGCGGTGATCCTGC
ACGCCCCGGCCACCGCGCGCGTCACCCGCGATGCCGATGGCGCCACGGTCGCGATCGCCGACGGGCCGAGCTATCGCGTG
AATCTGGTGGTCGCCGCCGACGGTCGGGGCAGCACGCTGCGCGCCGAGGCCGGCATCGCCGTCACCCGCTATCCCTATCG
TCAGAGCGCCGTGGTCTGTGCCGTGGAACACGAACGGCCGCACCACAACGTGGCGCTCGAGCATTTCCTGCCGGGCGGGC
CCTTCGCGCAACTGCCGATGAGCGATGCCGATGGCGCCCACCTGTCCGCCATCGTCTTCACCGAAAGCCACGCCAATGCC
GCGCGCCTCGCGGCAATGGACGATCCGCGATTCACCATCGAGGTCGCGCGGCGGCTCGGCGCGCATCTCGGCCGCATCCG
GCTGGTGGGGCGGCGCTGGACCTATCCGCTCTCGGCGCTGCACGCCCACCGCTATCATGCGACCCGGCTGGTTCTCGTCG
GCGATTCCGCGCACGGCGTGCACCCGATCGCCGGCCAGGGGCTCAATCTAGGCCTGCAGGACGGCATCGCGCTGGCCGAG
CTGATCGACGCGGCCGCCGATCCGGGCAGCCCGCACCTGCTGGCGCGCTATCAGCGCATCCGCCGGCCGGCCAACATCGC
CATGCTCGCCGCGACCGACGCACTCGACCGCCTGTTTTCGACCGACCTGCCGCCGGTGCGCCTCGCGCGCGATATCGGGC
TCGCCGCGGTGAACCGGATGCCCCGGCTCAAGCGCCGCTTCATGCGGACGGCGATGGGCCTGCGCGCATAG

Upstream 100 bases:

>100_bases
CCAGCCTCGGTCATGACCCAACACGCCGGCAGGCCAGCGCGGGCATGGCTTGCAGGATTCCCCGCAGATGCGACATTTCG
GCCATCACAGGAGGCCGGAC

Downstream 100 bases:

>100_bases
GGCCGCGCCGCAGCCCGGCAAGCCGCGATTCGATGCGCTTGCGGGTGCGGGTGATCAGCGCGACGCCGGCCAGCCGCCGG
TCGAGGAACGCCCTGGTGTC

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTNDTADVAIIGAGPVGAALALRLAAGGQRVVLVDRAALPPMEHPDFDGRAYAIAYGSRDLLDRAGLWDRLPFPPCPIER
IRVSDGKPGRPPSPLFLDFDHRDVGDEPFGWIVEARSLRMAINRALADSAVILHAPATARVTRDADGATVAIADGPSYRV
NLVVAADGRGSTLRAEAGIAVTRYPYRQSAVVCAVEHERPHHNVALEHFLPGGPFAQLPMSDADGAHLSAIVFTESHANA
ARLAAMDDPRFTIEVARRLGAHLGRIRLVGRRWTYPLSALHAHRYHATRLVLVGDSAHGVHPIAGQGLNLGLQDGIALAE
LIDAAADPGSPHLLARYQRIRRPANIAMLAATDALDRLFSTDLPPVRLARDIGLAAVNRMPRLKRRFMRTAMGLRA

Sequences:

>Translated_396_residues
MTNDTADVAIIGAGPVGAALALRLAAGGQRVVLVDRAALPPMEHPDFDGRAYAIAYGSRDLLDRAGLWDRLPFPPCPIER
IRVSDGKPGRPPSPLFLDFDHRDVGDEPFGWIVEARSLRMAINRALADSAVILHAPATARVTRDADGATVAIADGPSYRV
NLVVAADGRGSTLRAEAGIAVTRYPYRQSAVVCAVEHERPHHNVALEHFLPGGPFAQLPMSDADGAHLSAIVFTESHANA
ARLAAMDDPRFTIEVARRLGAHLGRIRLVGRRWTYPLSALHAHRYHATRLVLVGDSAHGVHPIAGQGLNLGLQDGIALAE
LIDAAADPGSPHLLARYQRIRRPANIAMLAATDALDRLFSTDLPPVRLARDIGLAAVNRMPRLKRRFMRTAMGLRA
>Mature_395_residues
TNDTADVAIIGAGPVGAALALRLAAGGQRVVLVDRAALPPMEHPDFDGRAYAIAYGSRDLLDRAGLWDRLPFPPCPIERI
RVSDGKPGRPPSPLFLDFDHRDVGDEPFGWIVEARSLRMAINRALADSAVILHAPATARVTRDADGATVAIADGPSYRVN
LVVAADGRGSTLRAEAGIAVTRYPYRQSAVVCAVEHERPHHNVALEHFLPGGPFAQLPMSDADGAHLSAIVFTESHANAA
RLAAMDDPRFTIEVARRLGAHLGRIRLVGRRWTYPLSALHAHRYHATRLVLVGDSAHGVHPIAGQGLNLGLQDGIALAEL
IDAAADPGSPHLLARYQRIRRPANIAMLAATDALDRLFSTDLPPVRLARDIGLAAVNRMPRLKRRFMRTAMGLRA

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=397, Percent_Identity=31.9899244332494, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI32967307, Length=346, Percent_Identity=32.9479768786127, Blast_Score=153, Evalue=2e-37,
Organism=Escherichia coli, GI1789274, Length=378, Percent_Identity=34.9206349206349, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1786883, Length=404, Percent_Identity=33.4158415841584, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1789273, Length=401, Percent_Identity=34.1645885286783, Blast_Score=177, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI25146631, Length=450, Percent_Identity=25.3333333333333, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321694, Length=293, Percent_Identity=27.3037542662116, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19920760, Length=432, Percent_Identity=28.0092592592593, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 42669; Mature: 42538

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDTADVAIIGAGPVGAALALRLAAGGQRVVLVDRAALPPMEHPDFDGRAYAIAYGSRD
CCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCHH
LLDRAGLWDRLPFPPCPIERIRVSDGKPGRPPSPLFLDFDHRDVGDEPFGWIVEARSLRM
HHHHCCCCCCCCCCCCCHHEEEECCCCCCCCCCCEEEECCCCCCCCCCCCEEHHHHHHHH
AINRALADSAVILHAPATARVTRDADGATVAIADGPSYRVNLVVAADGRGSTLRAEAGIA
HHHHHHCCCEEEEECCCCEEEEECCCCCEEEEECCCCEEEEEEEEECCCCCEEEECCCEE
VTRYPYRQSAVVCAVEHERPHHNVALEHFLPGGPFAQLPMSDADGAHLSAIVFTESHANA
EEECCCCCCEEEEEEECCCCCCCEEHHHCCCCCCEEECCCCCCCCCEEEEEEEECCCCCC
ARLAAMDDPRFTIEVARRLGAHLGRIRLVGRRWTYPLSALHAHRYHATRLVLVGDSAHGV
EEEEECCCCCHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHCEEEEEEEEEECCCCCC
HPIAGQGLNLGLQDGIALAELIDAAADPGSPHLLARYQRIRRPANIAMLAATDALDRLFS
CCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHH
TDLPPVRLARDIGLAAVNRMPRLKRRFMRTAMGLRA
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNDTADVAIIGAGPVGAALALRLAAGGQRVVLVDRAALPPMEHPDFDGRAYAIAYGSRD
CCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCHH
LLDRAGLWDRLPFPPCPIERIRVSDGKPGRPPSPLFLDFDHRDVGDEPFGWIVEARSLRM
HHHHCCCCCCCCCCCCCHHEEEECCCCCCCCCCCEEEECCCCCCCCCCCCEEHHHHHHHH
AINRALADSAVILHAPATARVTRDADGATVAIADGPSYRVNLVVAADGRGSTLRAEAGIA
HHHHHHCCCEEEEECCCCEEEEECCCCCEEEEECCCCEEEEEEEEECCCCCEEEECCCEE
VTRYPYRQSAVVCAVEHERPHHNVALEHFLPGGPFAQLPMSDADGAHLSAIVFTESHANA
EEECCCCCCEEEEEEECCCCCCCEEHHHCCCCCCEEECCCCCCCCCEEEEEEEECCCCCC
ARLAAMDDPRFTIEVARRLGAHLGRIRLVGRRWTYPLSALHAHRYHATRLVLVGDSAHGV
EEEEECCCCCHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHCEEEEEEEEEECCCCCC
HPIAGQGLNLGLQDGIALAELIDAAADPGSPHLLARYQRIRRPANIAMLAATDALDRLFS
CCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHH
TDLPPVRLARDIGLAAVNRMPRLKRRFMRTAMGLRA
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]