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The map label for this gene is caiD [H]

Identifier: 148259385

GI number: 148259385

Start: 420428

End: 421189

Strand: Reverse

Name: caiD [H]

Synonym: Acry_0368

Alternate gene names: 148259385

Gene position: 421189-420428 (Counterclockwise)

Preceding gene: 148259387

Following gene: 148259379

Centisome position: 12.43

GC content: 73.1

Gene sequence:

>762_bases
ATGACCGAAACACCCCCGATTCTCGAACGCCACGGCGCCATCGCCCGGCTGCGGCTGAACCGCCCCGCCCTGCGCAACCG
GCTCGGCGCGGACGATCTTGCCGTCCTGCTCGGCCATTGCGCCACGCTCGCGGCGGACGATTCGGTCCGCGTCGTCGTGT
TCGAGGCCGCCGGACCGGTCTTCAGCGCCGGCTTCGATCTCGATGCGCTCTCGCGCGGCGACACCGGCGATGCCGAGGAC
GGGCCGGGCGGCATCGGCCGCGTGGGCACGGCGCTCGCCGCCCTGCCCCAGCCGGTCATCGCCCGGCTGCACGGCAATCT
CTATGGCGGCGCGGTCGATCTCGCCCTCGCCTGCGATTTCCGCATCGGTGTGACCGGCATGGAACTGCTGATGCCGGCGG
CGCGGATCGGCCTGCATTACTACCGCTCTGGGCTCGCCCGCGCGGTCGAGCGGATCGGGCCGGACGCGACACGCCTGCTC
TTCGTCTCGGCGCAGACGATCGATGCGGCAACCCTGCTGCGGATCGGCTACCTGACCCAGCTGGCAGCCGCGGACGGGCT
GGATGCGGCGATCGACGCCCTCGCCGCCCGGCTGGAAGCCAATGCGCCCCTCGCCGTGCGCGGCATGAAGGCGGCGATCG
CCGGGCTCGCAGCCGGCACGCTGGATGGCGAATCGTTCGCCGCCGGCCTCGACGCCGCGCACCGCGACCCGGCCTTCATC
GAGGCCATGGGGGCGCTGCGGGCGAAGAAGCGCGGCGGCTGA

Upstream 100 bases:

>100_bases
GGGAGACGATCTCCCGGCTGAAGATGCGCCAGCGGCGCACAGCGGTCTTATAGTCCAAATGCCGCCGCTCCGCTACTGCG
GGGGCACGGAGGAGATCTGC

Downstream 100 bases:

>100_bases
GCTTCAGGCGCGGAGGTTGAAGGCCGCCAGCGCCGCGAGGTCGCGGTCGCCCCAGCCTTCGCGCACGGCGTCATGCGCCG
CGCGGCGGACCGATTCGACC

Product: enoyl-CoA hydratase/isomerase

Products: crotonoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTETPPILERHGAIARLRLNRPALRNRLGADDLAVLLGHCATLAADDSVRVVVFEAAGPVFSAGFDLDALSRGDTGDAED
GPGGIGRVGTALAALPQPVIARLHGNLYGGAVDLALACDFRIGVTGMELLMPAARIGLHYYRSGLARAVERIGPDATRLL
FVSAQTIDAATLLRIGYLTQLAAADGLDAAIDALAARLEANAPLAVRGMKAAIAGLAAGTLDGESFAAGLDAAHRDPAFI
EAMGALRAKKRGG

Sequences:

>Translated_253_residues
MTETPPILERHGAIARLRLNRPALRNRLGADDLAVLLGHCATLAADDSVRVVVFEAAGPVFSAGFDLDALSRGDTGDAED
GPGGIGRVGTALAALPQPVIARLHGNLYGGAVDLALACDFRIGVTGMELLMPAARIGLHYYRSGLARAVERIGPDATRLL
FVSAQTIDAATLLRIGYLTQLAAADGLDAAIDALAARLEANAPLAVRGMKAAIAGLAAGTLDGESFAAGLDAAHRDPAFI
EAMGALRAKKRGG
>Mature_252_residues
TETPPILERHGAIARLRLNRPALRNRLGADDLAVLLGHCATLAADDSVRVVVFEAAGPVFSAGFDLDALSRGDTGDAEDG
PGGIGRVGTALAALPQPVIARLHGNLYGGAVDLALACDFRIGVTGMELLMPAARIGLHYYRSGLARAVERIGPDATRLLF
VSAQTIDAATLLRIGYLTQLAAADGLDAAIDALAARLEANAPLAVRGMKAAIAGLAAGTLDGESFAAGLDAAHRDPAFIE
AMGALRAKKRGG

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Escherichia coli, GI221142681, Length=204, Percent_Identity=30.3921568627451, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787659, Length=210, Percent_Identity=27.1428571428571, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082183, Length=216, Percent_Identity=23.6111111111111, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=224, Percent_Identity=27.2321428571429, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=204, Percent_Identity=27.9411764705882, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.55

Molecular weight: Translated: 25977; Mature: 25846

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETPPILERHGAIARLRLNRPALRNRLGADDLAVLLGHCATLAADDSVRVVVFEAAGPV
CCCCCCHHHHCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCE
FSAGFDLDALSRGDTGDAEDGPGGIGRVGTALAALPQPVIARLHGNLYGGAVDLALACDF
EECCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEEECE
RIGVTGMELLMPAARIGLHYYRSGLARAVERIGPDATRLLFVSAQTIDAATLLRIGYLTQ
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHH
LAAADGLDAAIDALAARLEANAPLAVRGMKAAIAGLAAGTLDGESFAAGLDAAHRDPAFI
HHHCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHH
EAMGALRAKKRGG
HHHHHHHHHCCCC
>Mature Secondary Structure 
TETPPILERHGAIARLRLNRPALRNRLGADDLAVLLGHCATLAADDSVRVVVFEAAGPV
CCCCCHHHHCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCE
FSAGFDLDALSRGDTGDAEDGPGGIGRVGTALAALPQPVIARLHGNLYGGAVDLALACDF
EECCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEEECE
RIGVTGMELLMPAARIGLHYYRSGLARAVERIGPDATRLLFVSAQTIDAATLLRIGYLTQ
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHH
LAAADGLDAAIDALAARLEANAPLAVRGMKAAIAGLAAGTLDGESFAAGLDAAHRDPAFI
HHHCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHH
EAMGALRAKKRGG
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxybutanoyl-CoA

Specific reaction: (3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA