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The map label for this gene is eno

Identifier: 148259187

GI number: 148259187

Start: 193712

End: 194989

Strand: Reverse

Name: eno

Synonym: Acry_0167

Alternate gene names: 148259187

Gene position: 194989-193712 (Counterclockwise)

Preceding gene: 148259188

Following gene: 148259178

Centisome position: 5.75

GC content: 65.34

Gene sequence:

>1278_bases
ATGAGCGCCATCGCCGATATCACCGCACGGGAAATTCTCGACAGCCGCGGCAATCCGACCGTCGAGGTCGATGTCATCCT
GGATTCGGGCGCGATGGGACGCGCCGCTGTGCCCTCCGGTGCCTCGACCGGGGCGCATGAGGCCGTCGAGTTGCGCGATG
GTGAGCCGGCGCGGTTCGGCGGCAAGGGCGTGCAACGCGCGGTCGAGGCGGTCGAGGGCGAGATCTTCGATGCGATCGGC
GGGATGGATGCCAGCGAGCAGGTCGCAATCGACGAGACGATGATTGATCTCGACGGGACGCCCAACAAGGCCCGCCTGGG
GGCGAATGCGATTCTCGGCGTTTCGCTCGCCGTCGCCAAGGCGGCGGCGGACGAGATCGGGCTGCCGCTCTACCGCTATC
TCGGCGGAGTCTATGCCCGCACCCTGCCAGTGCCGATGATGAACATCATCAATGGTGGCAAGCACGCGGACAACCCGATC
GACATCCAGGAATTCATGATCCAGCCGGTGGGGGCCGCGAGCATCGCCGAGGCGGTGCGGATGGGCTCGGAAGTATTCCA
GGCGCTGAAGAAGATCCTGCACGATGCCGGGCACAACACCAATGTCGGTGATGAGGGCGGGTTCGCGCCTGGGCTGAAAT
CGGCCGAGGAGGCGCTGGGCTTCATGACGCGCGCCGTCGAGGCTGCTGGATACCGGGCCGGCGAGGACATTGCCTTCGCG
CTCGATTGCGCGGCGACCGAATTCTACAAGGACGGCAGGTATCACCTTGAGGGCGAGGGCAAGGTGCTCGATGCCGGGGG
GATGACCGACTATATCGCGGCACTAGCGAAGAGCTTTCCGATCATCTCGGTCGAGGATCCGCTTTCAGAGGATGACTGGG
AGGGCTGGGCGCATTTCACAAGCACACTCGGCGGCGCCATGCAGGTGGTGGGCGACGATCTGTTCGTCACCAACCCGACA
CGGCTGCGGCGCGGGATCGCGGCGAAGTCGGCCAATTCGATCCTGATCAAGGTGAACCAGATCGGCACGCTGAGCGAGAC
GCTGGAAGCGGTGGAACTGGCGCAGCGGGCGGGGATGACGGCGGTGATCAGCCATCGTTCGGGCGAGACCGAGGATGCGA
CCATCGCCGATATCGCGGTGGCGACCAATGCCGGGCAGATCAAGACCGGGTCGCTGGCGCGGTCGGACCGGGTGGCGAAG
TATAACCAGCTGATCCGCATCGAGGCGGAACTCGACATCGCGGGTCGCTTCGCCGGGCGGACCATTCTGCGCGGCTAA

Upstream 100 bases:

>100_bases
CAACGCGGCGGTCGAGGTCGAGGGGATGTTTTTGCTCCGCTGATTGCGCCGGGCCGCCCTTGCGCGTATGCCCCGGTTGC
ACTGCAAAAGGGGAGATCAG

Downstream 100 bases:

>100_bases
AGTATCATCCGATCGGATGGAATCATCTGATCGGATAAAGATGCTCAATTTATCAATTGGATGGAGCACTTTCCGATCCC
GGAGGATCGGGAAGTGCTCT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSAIADITAREILDSRGNPTVEVDVILDSGAMGRAAVPSGASTGAHEAVELRDGEPARFGGKGVQRAVEAVEGEIFDAIG
GMDASEQVAIDETMIDLDGTPNKARLGANAILGVSLAVAKAAADEIGLPLYRYLGGVYARTLPVPMMNIINGGKHADNPI
DIQEFMIQPVGAASIAEAVRMGSEVFQALKKILHDAGHNTNVGDEGGFAPGLKSAEEALGFMTRAVEAAGYRAGEDIAFA
LDCAATEFYKDGRYHLEGEGKVLDAGGMTDYIAALAKSFPIISVEDPLSEDDWEGWAHFTSTLGGAMQVVGDDLFVTNPT
RLRRGIAAKSANSILIKVNQIGTLSETLEAVELAQRAGMTAVISHRSGETEDATIADIAVATNAGQIKTGSLARSDRVAK
YNQLIRIEAELDIAGRFAGRTILRG

Sequences:

>Translated_425_residues
MSAIADITAREILDSRGNPTVEVDVILDSGAMGRAAVPSGASTGAHEAVELRDGEPARFGGKGVQRAVEAVEGEIFDAIG
GMDASEQVAIDETMIDLDGTPNKARLGANAILGVSLAVAKAAADEIGLPLYRYLGGVYARTLPVPMMNIINGGKHADNPI
DIQEFMIQPVGAASIAEAVRMGSEVFQALKKILHDAGHNTNVGDEGGFAPGLKSAEEALGFMTRAVEAAGYRAGEDIAFA
LDCAATEFYKDGRYHLEGEGKVLDAGGMTDYIAALAKSFPIISVEDPLSEDDWEGWAHFTSTLGGAMQVVGDDLFVTNPT
RLRRGIAAKSANSILIKVNQIGTLSETLEAVELAQRAGMTAVISHRSGETEDATIADIAVATNAGQIKTGSLARSDRVAK
YNQLIRIEAELDIAGRFAGRTILRG
>Mature_424_residues
SAIADITAREILDSRGNPTVEVDVILDSGAMGRAAVPSGASTGAHEAVELRDGEPARFGGKGVQRAVEAVEGEIFDAIGG
MDASEQVAIDETMIDLDGTPNKARLGANAILGVSLAVAKAAADEIGLPLYRYLGGVYARTLPVPMMNIINGGKHADNPID
IQEFMIQPVGAASIAEAVRMGSEVFQALKKILHDAGHNTNVGDEGGFAPGLKSAEEALGFMTRAVEAAGYRAGEDIAFAL
DCAATEFYKDGRYHLEGEGKVLDAGGMTDYIAALAKSFPIISVEDPLSEDDWEGWAHFTSTLGGAMQVVGDDLFVTNPTR
LRRGIAAKSANSILIKVNQIGTLSETLEAVELAQRAGMTAVISHRSGETEDATIADIAVATNAGQIKTGSLARSDRVAKY
NQLIRIEAELDIAGRFAGRTILRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=49.7685185185185, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=51.3888888888889, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=49.7685185185185, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=49.7685185185185, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=46.5116279069767, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.4880952380952, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.4880952380952, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.4880952380952, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=61.1627906976744, Blast_Score=499, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=50.462962962963, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=50.462962962963, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=44.7916666666667, Blast_Score=179, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321693, Length=438, Percent_Identity=48.6301369863014, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323985, Length=438, Percent_Identity=48.6301369863014, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324974, Length=438, Percent_Identity=48.4018264840183, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324969, Length=438, Percent_Identity=48.4018264840183, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321968, Length=438, Percent_Identity=47.4885844748858, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=441, Percent_Identity=48.5260770975057, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580916, Length=441, Percent_Identity=48.5260770975057, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580920, Length=441, Percent_Identity=48.5260770975057, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580914, Length=441, Percent_Identity=48.5260770975057, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281360527, Length=441, Percent_Identity=48.5260770975057, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17137654, Length=441, Percent_Identity=48.5260770975057, Blast_Score=385, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_ACICJ (A5FUW3)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001233314.1
- ProteinModelPortal:   A5FUW3
- SMR:   A5FUW3
- STRING:   A5FUW3
- GeneID:   5161216
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_0167
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- BioCyc:   ACRY349163:ACRY_0167-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44569; Mature: 44438

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIADITAREILDSRGNPTVEVDVILDSGAMGRAAVPSGASTGAHEAVELRDGEPARFG
CCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
GKGVQRAVEAVEGEIFDAIGGMDASEQVAIDETMIDLDGTPNKARLGANAILGVSLAVAK
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCCCCHHHCCCCHHHHHHHHHHH
AAADEIGLPLYRYLGGVYARTLPVPMMNIINGGKHADNPIDIQEFMIQPVGAASIAEAVR
HHHHHCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHH
MGSEVFQALKKILHDAGHNTNVGDEGGFAPGLKSAEEALGFMTRAVEAAGYRAGEDIAFA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
LDCAATEFYKDGRYHLEGEGKVLDAGGMTDYIAALAKSFPIISVEDPLSEDDWEGWAHFT
EHHHHHHHHHCCCEEECCCCEEEECCCHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
STLGGAMQVVGDDLFVTNPTRLRRGIAAKSANSILIKVNQIGTLSETLEAVELAQRAGMT
HHHHHHHHHHCCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDATIADIAVATNAGQIKTGSLARSDRVAKYNQLIRIEAELDIAGRFAGR
EEEECCCCCCCCCEEEEEEEECCCCCEECCCCHHHHHHHHHCCEEEEEEEEEEHHHHHCC
TILRG
HHCCC
>Mature Secondary Structure 
SAIADITAREILDSRGNPTVEVDVILDSGAMGRAAVPSGASTGAHEAVELRDGEPARFG
CCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
GKGVQRAVEAVEGEIFDAIGGMDASEQVAIDETMIDLDGTPNKARLGANAILGVSLAVAK
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCCCCHHHCCCCHHHHHHHHHHH
AAADEIGLPLYRYLGGVYARTLPVPMMNIINGGKHADNPIDIQEFMIQPVGAASIAEAVR
HHHHHCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHH
MGSEVFQALKKILHDAGHNTNVGDEGGFAPGLKSAEEALGFMTRAVEAAGYRAGEDIAFA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
LDCAATEFYKDGRYHLEGEGKVLDAGGMTDYIAALAKSFPIISVEDPLSEDDWEGWAHFT
EHHHHHHHHHCCCEEECCCCEEEECCCHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
STLGGAMQVVGDDLFVTNPTRLRRGIAAKSANSILIKVNQIGTLSETLEAVELAQRAGMT
HHHHHHHHHHCCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDATIADIAVATNAGQIKTGSLARSDRVAKYNQLIRIEAELDIAGRFAGR
EEEECCCCCCCCCEEEEEEEECCCCCEECCCCHHHHHHHHHCCEEEEEEEEEEHHHHHCC
TILRG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA