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The map label for this gene is gyrB [H]

Identifier: 148259050

GI number: 148259050

Start: 26325

End: 28286

Strand: Reverse

Name: gyrB [H]

Synonym: Acry_0029

Alternate gene names: 148259050

Gene position: 28286-26325 (Counterclockwise)

Preceding gene: 148259051

Following gene: 148259031

Centisome position: 0.83

GC content: 67.74

Gene sequence:

>1962_bases
GTGACCGATCTGTTCGAGCAGGCCGCCGGCCAGGCGAGCTATTCCGCCAAGGACATCGAGGTTCTCGAAGGGCTGGAACC
GGTTCGCCGGCGCCCCGGCATGTATATCGGCGGCACCGACGATGCGGCGATGCACCACCTCGCCGCCGAACTGCTCGACA
ACGCGATGGACGAGGCCGTAGCCGGCCACGCCTCGATCATCGAGATGACGCTTGCGCCCGGCAACCTGCTCACGGTGCGC
GATAACGGGCGCGGCATCCCGGTCGATCCGCACCCGAAATTCCGCGACAAGTCGGCACTCGAGGTGATTCTCACCACCCT
GCATTCCGGCGGCAAGTTCGCCGGCAAGGCCTATGCCACCTCGGGCGGTCTGCACGGCGTCGGCTCTTCGGTGGTGAACG
CACTGGCCGAGCGGCTGGAGGTCGAGGTCGCCCGCGAGAAAACCCTCTGGAAACAGGATTATGCCCGCGGCAAGCCGCTG
ACCAAGCTGGTCAATGCCGGGGCGACGCCGAACCGGCGTGGCACGCTGATCCGCTTCGTCCCCGATCCCGAGATTTTCGG
CGACAAGAAGTTCAGCCCGGCGCGGCTCTACCGCATGTGCCGCTCGAAGGCCTATCTGTTCCGCGGCGTGCGGATCCGCT
GGACGTGCGATGCGGCGCTGATCGCGGCGGGTGCCGATGTGCCGGCGCAGGCGGAGCTGCATTTCCCCGGCGGCCTCGCC
GATTCGCTGGCGGAGGAACTGGGCGCCGCGCCGAAGCTCGTCGCCCCGGTCTGGGCCGGGCAGGCCGACCTGCCGGACGA
TGCCGGGCGGATCGAGTGGGCCTGCGCCTGGCTGGAAGCCGGCGAGGCCACGCTCTCGACCTATTGCAACACGATCCCGA
CGACGCTCGGCGGCACGCATGAGGCGGGGTTCCGCGCCGCCCTGCTCAAGGGCCTGCGCGGCTTCGGCGAGCAGCGCAGC
AACAAGCGCGCCGCGGTGATCACCGCCGAGGATGCGACCGGCGCGCTCGCCGCCAAGCTCTCGCTGTTCATCCGCGACCC
GCAATTCCAGGGCCAGACCAAGGAAAAGCTGACCTCGCCCGAGGCGCAGAAACTGACCGAGGCGGCGCTGCGCGACCGGT
TCGAGCATTTTCTCACCGGCGATCCGGCGAACGCCGACAATCTTCTCGCCTTCGTGATCGAGCGGGCGGAGGAACGCATC
CGCCGCCGCGAGCAGAAGGACACGCCGCGCAAATCCGCCACGCGACGCCTTCGCCTGCCCGGCAAGCTGACCGATTGCTC
GCGCGAGAGCCGCGAGGATACCGAGATCTTTCTGGTCGAGGGCGATTCGGCTGGCGGCTCGGCCAAGCAGGCGCGCAACC
GCGAGACCCAGGCGGTGCTGCCGCTGCGCGGCAAGATCCTCAACGTCGCCTCCGCCTCCACGGAGAAGCTCCGGCAGAAC
CAGGAACTGAAAGACCTGATCGAGGCGCTGGGCTGCGGCGTCGGCGACCGGTTCCAGATCGACGCGCTGCGCTACGGCCG
GATCATCATCATGACCGACGCCGATGTTGACGGTGCTCACATCGCGTCGCTGCTGATGACGTTCTTCTACCGCGAGTTGC
CGGAACTGATCCGCGGCGGCCATGTCTATCTCGCCCAGCCGCCGCTCTACCGCCTCACCCAGGGCGGCAAGTCGGTCTAT
GCGATGGACGATGGCGAACGCGACCGGCTGGTGAAGACGGCGTTCAAGGCGAACGCCAAGGTCGAGATCAGCCGCTTCAA
GGGGCTTGGCGAGATGCCGCCGGCAATTCTCAAGGAAACCACGATGGACCCGAAGCGCCGCGTGCTGCTGCGCGTTCTCG
CCCCGCCCGAGGCACGAGCTGAAACGGCGGAAATCGTCGAGAGCCTGATGGGGCGGAAGCCCGAGCTGCGCTTCCGCTTC
ATCCAGGACCATGCCTCGAGCGTGCAGGAACTCGACGTCTGA

Upstream 100 bases:

>100_bases
ACGAGCCAAATGGATGCCAATCATGCCTTTACCGCGGCGGCCGATCTGGTGAACAAGGTACTCGCGCGGGGGTAACCCCG
CCCGGACCAGAGGAGACAGC

Downstream 100 bases:

>100_bases
CTCAGTTCATCATGCTCTTCATGAGCACACTGTTCGCCGTCAGGCGAAGGGCTCGGTCTCGCCGTCCGGCACGGGCACCC
GGAAGGCCTTGATGGTCATG

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 653; Mature: 652

Protein sequence:

>653_residues
MTDLFEQAAGQASYSAKDIEVLEGLEPVRRRPGMYIGGTDDAAMHHLAAELLDNAMDEAVAGHASIIEMTLAPGNLLTVR
DNGRGIPVDPHPKFRDKSALEVILTTLHSGGKFAGKAYATSGGLHGVGSSVVNALAERLEVEVAREKTLWKQDYARGKPL
TKLVNAGATPNRRGTLIRFVPDPEIFGDKKFSPARLYRMCRSKAYLFRGVRIRWTCDAALIAAGADVPAQAELHFPGGLA
DSLAEELGAAPKLVAPVWAGQADLPDDAGRIEWACAWLEAGEATLSTYCNTIPTTLGGTHEAGFRAALLKGLRGFGEQRS
NKRAAVITAEDATGALAAKLSLFIRDPQFQGQTKEKLTSPEAQKLTEAALRDRFEHFLTGDPANADNLLAFVIERAEERI
RRREQKDTPRKSATRRLRLPGKLTDCSRESREDTEIFLVEGDSAGGSAKQARNRETQAVLPLRGKILNVASASTEKLRQN
QELKDLIEALGCGVGDRFQIDALRYGRIIIMTDADVDGAHIASLLMTFFYRELPELIRGGHVYLAQPPLYRLTQGGKSVY
AMDDGERDRLVKTAFKANAKVEISRFKGLGEMPPAILKETTMDPKRRVLLRVLAPPEARAETAEIVESLMGRKPELRFRF
IQDHASSVQELDV

Sequences:

>Translated_653_residues
MTDLFEQAAGQASYSAKDIEVLEGLEPVRRRPGMYIGGTDDAAMHHLAAELLDNAMDEAVAGHASIIEMTLAPGNLLTVR
DNGRGIPVDPHPKFRDKSALEVILTTLHSGGKFAGKAYATSGGLHGVGSSVVNALAERLEVEVAREKTLWKQDYARGKPL
TKLVNAGATPNRRGTLIRFVPDPEIFGDKKFSPARLYRMCRSKAYLFRGVRIRWTCDAALIAAGADVPAQAELHFPGGLA
DSLAEELGAAPKLVAPVWAGQADLPDDAGRIEWACAWLEAGEATLSTYCNTIPTTLGGTHEAGFRAALLKGLRGFGEQRS
NKRAAVITAEDATGALAAKLSLFIRDPQFQGQTKEKLTSPEAQKLTEAALRDRFEHFLTGDPANADNLLAFVIERAEERI
RRREQKDTPRKSATRRLRLPGKLTDCSRESREDTEIFLVEGDSAGGSAKQARNRETQAVLPLRGKILNVASASTEKLRQN
QELKDLIEALGCGVGDRFQIDALRYGRIIIMTDADVDGAHIASLLMTFFYRELPELIRGGHVYLAQPPLYRLTQGGKSVY
AMDDGERDRLVKTAFKANAKVEISRFKGLGEMPPAILKETTMDPKRRVLLRVLAPPEARAETAEIVESLMGRKPELRFRF
IQDHASSVQELDV
>Mature_652_residues
TDLFEQAAGQASYSAKDIEVLEGLEPVRRRPGMYIGGTDDAAMHHLAAELLDNAMDEAVAGHASIIEMTLAPGNLLTVRD
NGRGIPVDPHPKFRDKSALEVILTTLHSGGKFAGKAYATSGGLHGVGSSVVNALAERLEVEVAREKTLWKQDYARGKPLT
KLVNAGATPNRRGTLIRFVPDPEIFGDKKFSPARLYRMCRSKAYLFRGVRIRWTCDAALIAAGADVPAQAELHFPGGLAD
SLAEELGAAPKLVAPVWAGQADLPDDAGRIEWACAWLEAGEATLSTYCNTIPTTLGGTHEAGFRAALLKGLRGFGEQRSN
KRAAVITAEDATGALAAKLSLFIRDPQFQGQTKEKLTSPEAQKLTEAALRDRFEHFLTGDPANADNLLAFVIERAEERIR
RREQKDTPRKSATRRLRLPGKLTDCSRESREDTEIFLVEGDSAGGSAKQARNRETQAVLPLRGKILNVASASTEKLRQNQ
ELKDLIEALGCGVGDRFQIDALRYGRIIIMTDADVDGAHIASLLMTFFYRELPELIRGGHVYLAQPPLYRLTQGGKSVYA
MDDGERDRLVKTAFKANAKVEISRFKGLGEMPPAILKETTMDPKRRVLLRVLAPPEARAETAEIVESLMGRKPELRFRFI
QDHASSVQELDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=663, Percent_Identity=25.1885369532428, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI19913408, Length=663, Percent_Identity=24.132730015083, Blast_Score=146, Evalue=7e-35,
Organism=Escherichia coli, GI1789408, Length=635, Percent_Identity=40.9448818897638, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI48994957, Length=563, Percent_Identity=42.4511545293073, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17535065, Length=645, Percent_Identity=23.1007751937985, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI212645845, Length=549, Percent_Identity=23.1329690346084, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=253, Percent_Identity=24.1106719367589, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=632, Percent_Identity=24.0506329113924, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17136538, Length=627, Percent_Identity=25.6778309409888, Blast_Score=135, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71479; Mature: 71348

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLFEQAAGQASYSAKDIEVLEGLEPVRRRPGMYIGGTDDAAMHHLAAELLDNAMDEAV
CCCHHHHHCCCCCCCHHHHHHHHCCHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
AGHASIIEMTLAPGNLLTVRDNGRGIPVDPHPKFRDKSALEVILTTLHSGGKFAGKAYAT
CCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEC
SGGLHGVGSSVVNALAERLEVEVAREKTLWKQDYARGKPLTKLVNAGATPNRRGTLIRFV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEC
PDPEIFGDKKFSPARLYRMCRSKAYLFRGVRIRWTCDAALIAAGADVPAQAELHFPGGLA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHEECCCCCCCCEEEECCCCHH
DSLAEELGAAPKLVAPVWAGQADLPDDAGRIEWACAWLEAGEATLSTYCNTIPTTLGGTH
HHHHHHHCCCCCEEHHHCCCCCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHCCCCCCCCC
EAGFRAALLKGLRGFGEQRSNKRAAVITAEDATGALAAKLSLFIRDPQFQGQTKEKLTSP
CHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHEEECCCCCCCHHHHHCCC
EAQKLTEAALRDRFEHFLTGDPANADNLLAFVIERAEERIRRREQKDTPRKSATRRLRLP
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEECCC
GKLTDCSRESREDTEIFLVEGDSAGGSAKQARNRETQAVLPLRGKILNVASASTEKLRQN
CCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCEEECCCCCCEEEHHHHHHHHHHCC
QELKDLIEALGCGVGDRFQIDALRYGRIIIMTDADVDGAHIASLLMTFFYRELPELIRGG
CHHHHHHHHHCCCCCCCEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
HVYLAQPPLYRLTQGGKSVYAMDDGERDRLVKTAFKANAKVEISRFKGLGEMPPAILKET
EEEEECCCHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCEEEEHHHCCCCCCCHHHHHHC
TMDPKRRVLLRVLAPPEARAETAEIVESLMGRKPELRFRFIQDHASSVQELDV
CCCHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDLFEQAAGQASYSAKDIEVLEGLEPVRRRPGMYIGGTDDAAMHHLAAELLDNAMDEAV
CCHHHHHCCCCCCCHHHHHHHHCCHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
AGHASIIEMTLAPGNLLTVRDNGRGIPVDPHPKFRDKSALEVILTTLHSGGKFAGKAYAT
CCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEC
SGGLHGVGSSVVNALAERLEVEVAREKTLWKQDYARGKPLTKLVNAGATPNRRGTLIRFV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEC
PDPEIFGDKKFSPARLYRMCRSKAYLFRGVRIRWTCDAALIAAGADVPAQAELHFPGGLA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHEECCCCCCCCEEEECCCCHH
DSLAEELGAAPKLVAPVWAGQADLPDDAGRIEWACAWLEAGEATLSTYCNTIPTTLGGTH
HHHHHHHCCCCCEEHHHCCCCCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHCCCCCCCCC
EAGFRAALLKGLRGFGEQRSNKRAAVITAEDATGALAAKLSLFIRDPQFQGQTKEKLTSP
CHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHEEECCCCCCCHHHHHCCC
EAQKLTEAALRDRFEHFLTGDPANADNLLAFVIERAEERIRRREQKDTPRKSATRRLRLP
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEECCC
GKLTDCSRESREDTEIFLVEGDSAGGSAKQARNRETQAVLPLRGKILNVASASTEKLRQN
CCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCEEECCCCCCEEEHHHHHHHHHHCC
QELKDLIEALGCGVGDRFQIDALRYGRIIIMTDADVDGAHIASLLMTFFYRELPELIRGG
CHHHHHHHHHCCCCCCCEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
HVYLAQPPLYRLTQGGKSVYAMDDGERDRLVKTAFKANAKVEISRFKGLGEMPPAILKET
EEEEECCCHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCEEEEHHHCCCCCCCHHHHHHC
TMDPKRRVLLRVLAPPEARAETAEIVESLMGRKPELRFRFIQDHASSVQELDV
CCCHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]