Click here to switch to the map view.

The map label for this gene is dnaB [H]

Identifier: 148243496

GI number: 148243496

Start: 2092831

End: 2094255

Strand: Direct

Name: dnaB [H]

Synonym: SynRCC307_2397

Alternate gene names: 148243496

Gene position: 2092831-2094255 (Clockwise)

Preceding gene: 148243495

Following gene: 148243497

Centisome position: 94.06

GC content: 61.82

Gene sequence:

>1425_bases
TTGTCCCTTGAACGACGGAACGGCGCGATGGTTTCTGTTCCCCTGAATGAACCAGAGAGGGAACGCACGCGCCGCAAAGA
TGCGGAACTGTTTGAGGCGACGCCCGAGCAGATTCCACCGCAGAACTTAGAAGCCGAGGAAATGATCCTCGGCGGCATCC
TGCTCGACCCCGATGCCATCGGCCGAGTCGCTGATGTGCTGCAACCGGAAGCCTTCTACACCGGTGCCCACCGGGAGATC
TTCCGCACCGCCTTGATGCTGCACGGCCAGGGCAAGCCCACCGACCTGACAGCGATGGTGGCCTGGCTCGCGGACACCGG
CCAGCTGGAGAAGGTGGGCGGCAGCAGCCGGCTCAGTGAGCTGGTGGACCGCACCCTCAGCACCGCCTCGATTGAAGAGG
TGGCCAAGCTGGTGATGGACAAGCACCTGCGCCGCCAGCTGATCCGCTCCGGCAATGAGGTGATTGGCCTTGGCTTTGAT
CAAAGCAAGCCCATGGATCAGGTGCTCGATGAAGCCGAGCAGAAGATCTTTGCGATCAGCCAGTCCAAACCGAGTCTGGG
GCTGACGCCCACCGCGGAAATCCTCACCCGCACCTTTGAAGAAATTGAAACCCGCTCATTAGGCACCTCGGTGGCGGGCA
TCCCGGTCAACTTCTACGACCTCGATGCGATGACCCAGGGCCTGCAACGCAGCGACCTGATCATCGTGGCCGGCCGCCCT
GCCATGGGTAAAACCTCGATCGTGCTGAACATCGCCAAGAACGTGGCACAACTGCACGACCTACCGGTCTGCGTCTTCAG
TTTGGAGATGAGCAAAGAGCAGCTCACCTACCGGCTGTTGTCGATGGAGGTGGGCATCGAAAGCGGACGGCTGCGCACCG
GCCGGCTCAATCAAGAGGAGTGGCCGCTGTTGGGCCAAGGCATCAGCACGCTGGGACGGCTGCCGATCTTCATCGACGAC
AAACCCAACGCCGGCGTGCTGGAAATGCGCTCGCTTTGCCGGCGCCTCATGGCTGAACAGGGCCGCGAGCTGGGCTTGAT
CGTGATCGACTACCTGCAGTTGATGGAAGGCTCCGGCTCCGATAACCGGGTGCAGGAGCTCTCGCGCATCACCCGGGGGC
TGAAGCAAATGGCGCGCGAACTGAATGTGCCGGTGATCGCCCTTTCCCAGCTCAGCCGCGGCGTGGAAGCGCGCACGAAC
AAGCGGCCGATGCTCAGTGACCTGCGGGAATCCGGCTCGATTGAGCAGGACGCCGACATCGTGATGATGATCTACCGCGA
TGAGTACTACAACCCGGAAACGCCCGATCGCGGCATCACCGAAGTGATCGTCACCAAGCACCGCAACGGCCCAGTTGGCA
CCGTGAAGTTGCTATTTGAAGCGCAGTTCACCCGCTTCCGCAACCTGGCGGCACCTGGGGGCTAA

Upstream 100 bases:

>100_bases
CTACAAGGTCCAGGTCAAGCTGCACCCTGAAGTGGTCGCCGAGATCAATCTGGAAGTGGTCAGCCACTAAGGCCAAACCG
CCCGAAACGGGGCAGCATGG

Downstream 100 bases:

>100_bases
CCAAACGACTCATGTGGATCGGGCGGATCTTGCTGCTGGGTTATCTGTCTTCGGTGGTGGCTCGTTGGACGATGAGTCCT
GCTGCACACCGCCGTTATGT

Product: replicative DNA helicase

Products: NA

Alternate protein names: Asp dnaB intein [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSLERRNGAMVSVPLNEPERERTRRKDAELFEATPEQIPPQNLEAEEMILGGILLDPDAIGRVADVLQPEAFYTGAHREI
FRTALMLHGQGKPTDLTAMVAWLADTGQLEKVGGSSRLSELVDRTLSTASIEEVAKLVMDKHLRRQLIRSGNEVIGLGFD
QSKPMDQVLDEAEQKIFAISQSKPSLGLTPTAEILTRTFEEIETRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGRP
AMGKTSIVLNIAKNVAQLHDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLNQEEWPLLGQGISTLGRLPIFIDD
KPNAGVLEMRSLCRRLMAEQGRELGLIVIDYLQLMEGSGSDNRVQELSRITRGLKQMARELNVPVIALSQLSRGVEARTN
KRPMLSDLRESGSIEQDADIVMMIYRDEYYNPETPDRGITEVIVTKHRNGPVGTVKLLFEAQFTRFRNLAAPGG

Sequences:

>Translated_474_residues
MSLERRNGAMVSVPLNEPERERTRRKDAELFEATPEQIPPQNLEAEEMILGGILLDPDAIGRVADVLQPEAFYTGAHREI
FRTALMLHGQGKPTDLTAMVAWLADTGQLEKVGGSSRLSELVDRTLSTASIEEVAKLVMDKHLRRQLIRSGNEVIGLGFD
QSKPMDQVLDEAEQKIFAISQSKPSLGLTPTAEILTRTFEEIETRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGRP
AMGKTSIVLNIAKNVAQLHDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLNQEEWPLLGQGISTLGRLPIFIDD
KPNAGVLEMRSLCRRLMAEQGRELGLIVIDYLQLMEGSGSDNRVQELSRITRGLKQMARELNVPVIALSQLSRGVEARTN
KRPMLSDLRESGSIEQDADIVMMIYRDEYYNPETPDRGITEVIVTKHRNGPVGTVKLLFEAQFTRFRNLAAPGG
>Mature_473_residues
SLERRNGAMVSVPLNEPERERTRRKDAELFEATPEQIPPQNLEAEEMILGGILLDPDAIGRVADVLQPEAFYTGAHREIF
RTALMLHGQGKPTDLTAMVAWLADTGQLEKVGGSSRLSELVDRTLSTASIEEVAKLVMDKHLRRQLIRSGNEVIGLGFDQ
SKPMDQVLDEAEQKIFAISQSKPSLGLTPTAEILTRTFEEIETRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGRPA
MGKTSIVLNIAKNVAQLHDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLNQEEWPLLGQGISTLGRLPIFIDDK
PNAGVLEMRSLCRRLMAEQGRELGLIVIDYLQLMEGSGSDNRVQELSRITRGLKQMARELNVPVIALSQLSRGVEARTNK
RPMLSDLRESGSIEQDADIVMMIYRDEYYNPETPDRGITEVIVTKHRNGPVGTVKLLFEAQFTRFRNLAAPGG

Specific function: The intein is an endonuclease (Potential) [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=459, Percent_Identity=43.1372549019608, Blast_Score=353, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52645; Mature: 52514

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLERRNGAMVSVPLNEPERERTRRKDAELFEATPEQIPPQNLEAEEMILGGILLDPDAI
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCEEECCHHH
GRVADVLQPEAFYTGAHREIFRTALMLHGQGKPTDLTAMVAWLADTGQLEKVGGSSRLSE
HHHHHHHCCHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCCCHHHHHH
LVDRTLSTASIEEVAKLVMDKHLRRQLIRSGNEVIGLGFDQSKPMDQVLDEAEQKIFAIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHEEEEE
QSKPSLGLTPTAEILTRTFEEIETRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGRP
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCCCCCCEEEEECCC
AMGKTSIVLNIAKNVAQLHDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLNQEE
CCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEECCCCCCCC
WPLLGQGISTLGRLPIFIDDKPNAGVLEMRSLCRRLMAEQGRELGLIVIDYLQLMEGSGS
CCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
DNRVQELSRITRGLKQMARELNVPVIALSQLSRGVEARTNKRPMLSDLRESGSIEQDADI
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHCCCCCHHHHHHHCCCCCCCCCE
VMMIYRDEYYNPETPDRGITEVIVTKHRNGPVGTVKLLFEAQFTRFRNLAAPGG
EEEEEECCCCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SLERRNGAMVSVPLNEPERERTRRKDAELFEATPEQIPPQNLEAEEMILGGILLDPDAI
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCEEECCHHH
GRVADVLQPEAFYTGAHREIFRTALMLHGQGKPTDLTAMVAWLADTGQLEKVGGSSRLSE
HHHHHHHCCHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCCCHHHHHH
LVDRTLSTASIEEVAKLVMDKHLRRQLIRSGNEVIGLGFDQSKPMDQVLDEAEQKIFAIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHEEEEE
QSKPSLGLTPTAEILTRTFEEIETRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGRP
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCCCCCCEEEEECCC
AMGKTSIVLNIAKNVAQLHDLPVCVFSLEMSKEQLTYRLLSMEVGIESGRLRTGRLNQEE
CCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEECCCCCCCC
WPLLGQGISTLGRLPIFIDDKPNAGVLEMRSLCRRLMAEQGRELGLIVIDYLQLMEGSGS
CCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
DNRVQELSRITRGLKQMARELNVPVIALSQLSRGVEARTNKRPMLSDLRESGSIEQDADI
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHCCCCCHHHHHHHCCCCCCCCCE
VMMIYRDEYYNPETPDRGITEVIVTKHRNGPVGTVKLLFEAQFTRFRNLAAPGG
EEEEEECCCCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]