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The map label for this gene is leuC

Identifier: 148243430

GI number: 148243430

Start: 2027695

End: 2029104

Strand: Direct

Name: leuC

Synonym: SynRCC307_2331

Alternate gene names: 148243430

Gene position: 2027695-2029104 (Clockwise)

Preceding gene: 148243429

Following gene: 148243431

Centisome position: 91.14

GC content: 64.04

Gene sequence:

>1410_bases
TTGAGCACAGCCACCCTCTACGACAAGGTCTGGGCCCTGCATCAGGTGGCTGAGCTGCCCAGCGGGTCGACCCAGCTGTT
CATTGGCCTGCACCTGATCCATGAGGTCACCAGTCCGCAAGCCTTTGCTGCCCTGAAGGATCTGGGGCTGAGTGTCCGCC
ACCCCGAACGCACGGTGGCCACGGTGGACCACATCGTGCCAACCACAAGCCAAGCGCGGCCCTTCGCCGATGGACTGGCC
GAAGAAATGCTCAGCACCCTGGAGCGCAACTGCCATGAGAACGGCATCCATCTCAATGGCCTTGGCAGTGGCCGCCAAGG
AATCGTCCATGTGATGGCCCCAGAGCTGGGCTTGACCCAACCGGGCATGACCGTGGCCTGCGGTGATTCGCACACCTCCA
CCCACGGCGCCTTTGGTGCCATTGCCTTTGGCATTGGCACCAGCCAAGTGCGCGATGTCTTGGCCAGCCAAAGCTTGACG
ATGAACAAGCTCAAGGTGCGCCGCATCTGGGTGGATGGAGCGCTGCAACCCGGCGTCTTTGCCAAAGATCTGGTGCTGCA
CATCATCCGCACCCTTGGCGTTAAAGGCGGTGTGGGCTACGCCTATGAATTCGCCGGGCCTGCGATCGAAGCCCTCTCGA
TGGAGGAGCGCATGACCCTCTGCAACATGGCGATCGAAGGCGGCGCCCGCTGCGGCTATGTCAATCCCGATCAAACCACG
TTTGACTACCTCAAGGGCCGCCCCCATGCGCCCAGCGGTGATGCCTGGGATCACGCTGTGAGCTGGTGGAAGAGTTTGGC
CAGCGGAGCGGACGCCTGCTTCGACGATGAGGTGAAGTTCGATGCCGCCGTCATCGCCCCCACCATCACCTGGGGAATCA
CCCCAGGCCAGGGCATTGGCGTGGATGAGGCGGTACCCACCTTGGAGCAAACCCCCGAGGAAGACCGCCCCCTGGCACAA
GAGGCTTACCGCTACATGGATTTGCAGCCGGGCCAAGCGATTGCCGGGCTGCCTGTGGATGTCTGTTTCATTGGCAGCTG
CACCAATGGCCGCCTCAGCGACCTGCGCGCGGCAGCCGCTGTTGCCGCTGGCCGCCAGGTGGCCTCAGGCATCAAGGCAT
TTGTCGTGCCGGGATCGGAGCAAGTGGCCGCCGCCGCCGAAGCCGAAGGCCTGGATGCTGTGTTCCGCCAAGCGGGCTTC
GAATGGCGCGAGCCGGGCTGCTCGATGTGCCTAGCGATGAACCCCGATCGCCTCGAAGGCCGCCAGATCAGCGCCAGCTC
CAGCAACCGCAACTTCAAAGGCCGGCAAGGCTCAGCCAGCGGCCGCACGCTGCTGATGAGCCCGGCGATGGTGGCTGCCG
CTGCCATTGCGGGCCGTGTCACCGACGTTCGCTCCCTGCCGCCTGCTTAA

Upstream 100 bases:

>100_bases
GCCGCGGCCGCGATTGGATCCGCGGGCTCAGCGTGGGGGACGCGTTGAATCTGTTGCGGCTGCAGCTTTAGGCTGCTCTG
TTTGGCGAAGGTAAAAGCGC

Downstream 100 bases:

>100_bases
ACACCCATGACATCAACTCCTTTTCCCAGCGGCGCAATCAGCCAAGTCAGCGGACGCACCGTGGTGCTGCGCGGCAACGA
CATCGACACCGACCGAATCA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSTATLYDKVWALHQVAELPSGSTQLFIGLHLIHEVTSPQAFAALKDLGLSVRHPERTVATVDHIVPTTSQARPFADGLA
EEMLSTLERNCHENGIHLNGLGSGRQGIVHVMAPELGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLT
MNKLKVRRIWVDGALQPGVFAKDLVLHIIRTLGVKGGVGYAYEFAGPAIEALSMEERMTLCNMAIEGGARCGYVNPDQTT
FDYLKGRPHAPSGDAWDHAVSWWKSLASGADACFDDEVKFDAAVIAPTITWGITPGQGIGVDEAVPTLEQTPEEDRPLAQ
EAYRYMDLQPGQAIAGLPVDVCFIGSCTNGRLSDLRAAAAVAAGRQVASGIKAFVVPGSEQVAAAAEAEGLDAVFRQAGF
EWREPGCSMCLAMNPDRLEGRQISASSSNRNFKGRQGSASGRTLLMSPAMVAAAAIAGRVTDVRSLPPA

Sequences:

>Translated_469_residues
MSTATLYDKVWALHQVAELPSGSTQLFIGLHLIHEVTSPQAFAALKDLGLSVRHPERTVATVDHIVPTTSQARPFADGLA
EEMLSTLERNCHENGIHLNGLGSGRQGIVHVMAPELGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLT
MNKLKVRRIWVDGALQPGVFAKDLVLHIIRTLGVKGGVGYAYEFAGPAIEALSMEERMTLCNMAIEGGARCGYVNPDQTT
FDYLKGRPHAPSGDAWDHAVSWWKSLASGADACFDDEVKFDAAVIAPTITWGITPGQGIGVDEAVPTLEQTPEEDRPLAQ
EAYRYMDLQPGQAIAGLPVDVCFIGSCTNGRLSDLRAAAAVAAGRQVASGIKAFVVPGSEQVAAAAEAEGLDAVFRQAGF
EWREPGCSMCLAMNPDRLEGRQISASSSNRNFKGRQGSASGRTLLMSPAMVAAAAIAGRVTDVRSLPPA
>Mature_468_residues
STATLYDKVWALHQVAELPSGSTQLFIGLHLIHEVTSPQAFAALKDLGLSVRHPERTVATVDHIVPTTSQARPFADGLAE
EMLSTLERNCHENGIHLNGLGSGRQGIVHVMAPELGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLTM
NKLKVRRIWVDGALQPGVFAKDLVLHIIRTLGVKGGVGYAYEFAGPAIEALSMEERMTLCNMAIEGGARCGYVNPDQTTF
DYLKGRPHAPSGDAWDHAVSWWKSLASGADACFDDEVKFDAAVIAPTITWGITPGQGIGVDEAVPTLEQTPEEDRPLAQE
AYRYMDLQPGQAIAGLPVDVCFIGSCTNGRLSDLRAAAAVAAGRQVASGIKAFVVPGSEQVAAAAEAEGLDAVFRQAGFE
WREPGCSMCLAMNPDRLEGRQISASSSNRNFKGRQGSASGRTLLMSPAMVAAAAIAGRVTDVRSLPPA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=364, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI8659555, Length=451, Percent_Identity=25.0554323725055, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=53.1317494600432, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1787531, Length=367, Percent_Identity=27.2479564032698, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25149337, Length=413, Percent_Identity=27.6029055690073, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI32564738, Length=413, Percent_Identity=27.6029055690073, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI25149342, Length=321, Percent_Identity=28.0373831775701, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17568399, Length=379, Percent_Identity=25.5936675461741, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=52.5531914893617, Blast_Score=463, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322261, Length=403, Percent_Identity=28.0397022332506, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323335, Length=375, Percent_Identity=29.6, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6320440, Length=439, Percent_Identity=26.6514806378132, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281365315, Length=394, Percent_Identity=27.9187817258883, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17864292, Length=394, Percent_Identity=27.9187817258883, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161076999, Length=394, Percent_Identity=27.9187817258883, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645686, Length=376, Percent_Identity=27.6595744680851, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28571643, Length=396, Percent_Identity=26.2626262626263, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=27.7628032345013, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_SYNR3 (A5GWH5)

Other databases:

- EMBL:   CT978603
- RefSeq:   YP_001228587.1
- ProteinModelPortal:   A5GWH5
- SMR:   A5GWH5
- STRING:   A5GWH5
- GeneID:   5156578
- GenomeReviews:   CT978603_GR
- KEGG:   syr:SynRCC307_2331
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   TLERNCA
- ProtClustDB:   PRK05478
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 49586; Mature: 49455

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTATLYDKVWALHQVAELPSGSTQLFIGLHLIHEVTSPQAFAALKDLGLSVRHPERTVA
CCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHH
TVDHIVPTTSQARPFADGLAEEMLSTLERNCHENGIHLNGLGSGRQGIVHVMAPELGLTQ
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEECCCCCCCC
PGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLTMNKLKVRRIWVDGALQPGVF
CCCEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHCCCCHHHEEEEEEEECCCCCCCCH
AKDLVLHIIRTLGVKGGVGYAYEFAGPAIEALSMEERMTLCNMAIEGGARCGYVNPDQTT
HHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
FDYLKGRPHAPSGDAWDHAVSWWKSLASGADACFDDEVKFDAAVIAPTITWGITPGQGIG
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECEEEECCCCCCCCC
VDEAVPTLEQTPEEDRPLAQEAYRYMDLQPGQAIAGLPVDVCFIGSCTNGRLSDLRAAAA
CHHHCCCHHCCCCCCCCHHHHHHHHHCCCCCCEECCCCEEEEEEECCCCCCHHHHHHHHH
VAAGRQVASGIKAFVVPGSEQVAAAAEAEGLDAVFRQAGFEWREPGCSMCLAMNPDRLEG
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCC
RQISASSSNRNFKGRQGSASGRTLLMSPAMVAAAAIAGRVTDVRSLPPA
CEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STATLYDKVWALHQVAELPSGSTQLFIGLHLIHEVTSPQAFAALKDLGLSVRHPERTVA
CCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHH
TVDHIVPTTSQARPFADGLAEEMLSTLERNCHENGIHLNGLGSGRQGIVHVMAPELGLTQ
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEECCCCCCCC
PGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQSLTMNKLKVRRIWVDGALQPGVF
CCCEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHCCCCHHHEEEEEEEECCCCCCCCH
AKDLVLHIIRTLGVKGGVGYAYEFAGPAIEALSMEERMTLCNMAIEGGARCGYVNPDQTT
HHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
FDYLKGRPHAPSGDAWDHAVSWWKSLASGADACFDDEVKFDAAVIAPTITWGITPGQGIG
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECEEEECCCCCCCCC
VDEAVPTLEQTPEEDRPLAQEAYRYMDLQPGQAIAGLPVDVCFIGSCTNGRLSDLRAAAA
CHHHCCCHHCCCCCCCCHHHHHHHHHCCCCCCEECCCCEEEEEEECCCCCCHHHHHHHHH
VAAGRQVASGIKAFVVPGSEQVAAAAEAEGLDAVFRQAGFEWREPGCSMCLAMNPDRLEG
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCC
RQISASSSNRNFKGRQGSASGRTLLMSPAMVAAAAIAGRVTDVRSLPPA
CEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA