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The map label for this gene is mraW

Identifier: 148243357

GI number: 148243357

Start: 1963780

End: 1964679

Strand: Direct

Name: mraW

Synonym: SynRCC307_2258

Alternate gene names: 148243357

Gene position: 1963780-1964679 (Clockwise)

Preceding gene: 148243354

Following gene: 148243359

Centisome position: 88.26

GC content: 65.22

Gene sequence:

>900_bases
TTGAGCAGTTCCTTTCATCACCAGCCGGTGCTGCCGCAACAGGTGCTGGAAGCACTAGCCGAATTGCCCGATGAAGGCGT
GCTGCTCGATGCCACCGTGGGCGGCGGCGGACACAGCAGCCTGCTGCTGGATGTCCACCCCGGCTGGCAGCTGATCGGCC
TGGATCAAGACCCCGCCGCCCGGGCCGCAGCGGCCCAACAGCTGGAGCGTTTTGGCGAGCGGGTGCAGCTGGTGGCCAGC
AACTTTGCGGCTTATACCCCAGATCAACCGGTGGTGGCGGTGCTGGCTGATCTGGGCGTCAGCAGCCATCAGCTCGATGT
GCCCGAGCGCGGCTTCAGCTTCCGCGCCGATGGCCCTCTGGACATGCGCATGAACACCGAAGGCGACGGAGAAACTGCCG
CAGCGCTGATCGACCGCCTGGAGGAAAACGCCCTAGCCGATCTGCTGTTTCACTACGGCGAGGAGCGACTTTCGAGGCGG
ATTGCGCGCCGGCTCAAAACTGAAGGCCCCTGGGATGACGGGGAAAGGGGCACCGCAGCGCTGGCCTATGCGATCGCCGG
CTGCTACCCGCCCAAGCAACGCCATGGCCGCATCCATGCCGCCACCCGCAGCTTCCAGGCCTTACGGATTGCGGTGAACG
ATGAGCTGGGGGTGCTGGAAACCCTGCTCAACGACGCTCCCAACTGGCTCGAGCCCGGTGGGCGGATCGCCGTGATCAGC
TTTCACTCGCTCGAGGATCGGCTGGTCAAAAACCGCTTTAAGGCCGATGAGCGTCTGCGGGTGATCAGCCGCAAGCCCTT
GATCGCTTCAGAGCAGGAAGCCGAGGCCAACCCCCGGGCCCGCTCCGCCAAGTTGCGCGTCGCCGAGCGCCTCAGCGCAG
AGCTTGAACCTGCTCAGTGA

Upstream 100 bases:

>100_bases
AGCACCATCGGCTCGGTGCGCGTTTCCAGCTGCGTCATGGGGGCCGGGGGCCGAAGCTGCGCTGATCCTAGGAAGGACCC
TGTGCCATCCCCTTGCGGAC

Downstream 100 bases:

>100_bases
ACCAAGCCAGGGCCTGATCCATCTGCTCGGCCGTGTTGGCGCGCCCCAAACCAAACCGAACCGACGCTGCGGCCTGCTGC
CGGCTGCGGCCCAAGGCGAG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSSSFHHQPVLPQQVLEALAELPDEGVLLDATVGGGGHSSLLLDVHPGWQLIGLDQDPAARAAAAQQLERFGERVQLVAS
NFAAYTPDQPVVAVLADLGVSSHQLDVPERGFSFRADGPLDMRMNTEGDGETAAALIDRLEENALADLLFHYGEERLSRR
IARRLKTEGPWDDGERGTAALAYAIAGCYPPKQRHGRIHAATRSFQALRIAVNDELGVLETLLNDAPNWLEPGGRIAVIS
FHSLEDRLVKNRFKADERLRVISRKPLIASEQEAEANPRARSAKLRVAERLSAELEPAQ

Sequences:

>Translated_299_residues
MSSSFHHQPVLPQQVLEALAELPDEGVLLDATVGGGGHSSLLLDVHPGWQLIGLDQDPAARAAAAQQLERFGERVQLVAS
NFAAYTPDQPVVAVLADLGVSSHQLDVPERGFSFRADGPLDMRMNTEGDGETAAALIDRLEENALADLLFHYGEERLSRR
IARRLKTEGPWDDGERGTAALAYAIAGCYPPKQRHGRIHAATRSFQALRIAVNDELGVLETLLNDAPNWLEPGGRIAVIS
FHSLEDRLVKNRFKADERLRVISRKPLIASEQEAEANPRARSAKLRVAERLSAELEPAQ
>Mature_298_residues
SSSFHHQPVLPQQVLEALAELPDEGVLLDATVGGGGHSSLLLDVHPGWQLIGLDQDPAARAAAAQQLERFGERVQLVASN
FAAYTPDQPVVAVLADLGVSSHQLDVPERGFSFRADGPLDMRMNTEGDGETAAALIDRLEENALADLLFHYGEERLSRRI
ARRLKTEGPWDDGERGTAALAYAIAGCYPPKQRHGRIHAATRSFQALRIAVNDELGVLETLLNDAPNWLEPGGRIAVISF
HSLEDRLVKNRFKADERLRVISRKPLIASEQEAEANPRARSAKLRVAERLSAELEPAQ

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=339, Percent_Identity=37.4631268436578, Blast_Score=177, Evalue=7e-45,
Organism=Homo sapiens, GI165377202, Length=167, Percent_Identity=40.1197604790419, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1786270, Length=323, Percent_Identity=38.6996904024768, Blast_Score=174, Evalue=6e-45,
Organism=Drosophila melanogaster, GI62472493, Length=327, Percent_Identity=31.8042813455657, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28571637, Length=327, Percent_Identity=31.8042813455657, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_SYNR3 (A5GWA2)

Other databases:

- EMBL:   CT978603
- RefSeq:   YP_001228514.1
- ProteinModelPortal:   A5GWA2
- STRING:   A5GWA2
- GeneID:   5156689
- GenomeReviews:   CT978603_GR
- KEGG:   syr:SynRCC307_2258
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 32718; Mature: 32587

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 82-82 BINDING 97-97 BINDING 104-104

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSFHHQPVLPQQVLEALAELPDEGVLLDATVGGGGHSSLLLDVHPGWQLIGLDQDPAA
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCEEEEECCCCHHH
RAAAAQQLERFGERVQLVASNFAAYTPDQPVVAVLADLGVSSHQLDVPERGFSFRADGPL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCE
DMRMNTEGDGETAAALIDRLEENALADLLFHYGEERLSRRIARRLKTEGPWDDGERGTAA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LAYAIAGCYPPKQRHGRIHAATRSFQALRIAVNDELGVLETLLNDAPNWLEPGGRIAVIS
HHHHHHHCCCCHHHCCEEHHHHHCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEE
FHSLEDRLVKNRFKADERLRVISRKPLIASEQEAEANPRARSAKLRVAERLSAELEPAQ
EHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSSFHHQPVLPQQVLEALAELPDEGVLLDATVGGGGHSSLLLDVHPGWQLIGLDQDPAA
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCEEEEECCCCHHH
RAAAAQQLERFGERVQLVASNFAAYTPDQPVVAVLADLGVSSHQLDVPERGFSFRADGPL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCE
DMRMNTEGDGETAAALIDRLEENALADLLFHYGEERLSRRIARRLKTEGPWDDGERGTAA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LAYAIAGCYPPKQRHGRIHAATRSFQALRIAVNDELGVLETLLNDAPNWLEPGGRIAVIS
HHHHHHHCCCCHHHCCEEHHHHHCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEE
FHSLEDRLVKNRFKADERLRVISRKPLIASEQEAEANPRARSAKLRVAERLSAELEPAQ
EHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA