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The map label for this gene is gshB [H]

Identifier: 148243350

GI number: 148243350

Start: 1956763

End: 1957686

Strand: Direct

Name: gshB [H]

Synonym: SynRCC307_2251

Alternate gene names: 148243350

Gene position: 1956763-1957686 (Clockwise)

Preceding gene: 148243349

Following gene: 148243352

Centisome position: 87.95

GC content: 65.37

Gene sequence:

>924_bases
GTGGGGCGCCAACTCTTTCTGATTGATCCGATTGGGCGCCTGCGCCCTGAAAAAGACAGCAGCGTTGCGCTGATGCAGGC
CGGACAGCGCGCCGGCGAAGAGATCTGGGCTGCTCAACCCAGCGATCTGGCAGCCGGGGAAGGCGGCCCCCGCGTGCTGG
CCGCGCCGATCACTGCCGAGCCTTGGTACAGCGCAGGGGAAGCCCGCTGGCAGCCCCTCAGCCAGTTCCAGCAGGTGTGG
ATGCGCAAAGACCCACCGGTGGATGAGGCCTACCTCTATGCCACCCACTTGCTTGAGCTGGCGGAGCGCCAGGGCGTGCA
GGTGCTCAACCGCCCGGCCAGCCTGCGGGCCTGGAACGAAAAGCTGGGGGCGCTGCAGTTCCCAGAGCTGATGGCCCCCA
CCCTGGTGGCCAGCGATGTGGAGCTGTTGCGTCAATTTGCCGCCACCCATGGAGAGGTGGTGCTCAAGCCCCTCGGTGGG
CGCGCTGGTCAGGGGGTAATCCGCTCCAGTGGCGAGGCTCCAGGGCTAGGAGCCCTGCTGGAGCTGGTCACGCAGCAGCA
GCAACTGCCAGTGATGATCCAGGCGTTTTTGCCCCAGGTCAGTGAGGGCGATAAGCGCATCTTGCTGGTGAACGGCGAGC
CGCTGGGCGCCGTCAACCGCAAACCGAAGGCGGGTGAATTCCGCAGCAACCTGGCTGTGGGCGGCCAACCGGAAGCCACC
GACCTCAGCCCCCGCGAGCGGCAGATCTGCGATGTGTTGCGGCCCGCCTTAATTGAGGCCGGACTGTTCTTTGTCGGCAT
CGATGTGATCGCTGGTCACCTCAGCGAGATCAATGTCACCAGCCCCACCGGCATCCGCGAAGTGGAGCAGCTCGGGAAGA
TTCCCCTGGCTGATCTCACCCAGCAGCGTTTACTGCAGGGCTGA

Upstream 100 bases:

>100_bases
CATCAACGGGCAACACGTGGGTGGCTGCGATGACCTCTATGCCCTTGAACGCAGCGGCCAACTGGATGGCCTGCTGGCCT
GATCACGGAGCCAGATCAGC

Downstream 100 bases:

>100_bases
CGGTGAGCTGCTGCTGCAGCACCTCAAGCTTGAGGGCCACCTCCTGCAATTCCCGCTCAGGCACCGAGCCCTTGACCAAA
CCAGCTCCTGCGGTGAGCTC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MGRQLFLIDPIGRLRPEKDSSVALMQAGQRAGEEIWAAQPSDLAAGEGGPRVLAAPITAEPWYSAGEARWQPLSQFQQVW
MRKDPPVDEAYLYATHLLELAERQGVQVLNRPASLRAWNEKLGALQFPELMAPTLVASDVELLRQFAATHGEVVLKPLGG
RAGQGVIRSSGEAPGLGALLELVTQQQQLPVMIQAFLPQVSEGDKRILLVNGEPLGAVNRKPKAGEFRSNLAVGGQPEAT
DLSPRERQICDVLRPALIEAGLFFVGIDVIAGHLSEINVTSPTGIREVEQLGKIPLADLTQQRLLQG

Sequences:

>Translated_307_residues
MGRQLFLIDPIGRLRPEKDSSVALMQAGQRAGEEIWAAQPSDLAAGEGGPRVLAAPITAEPWYSAGEARWQPLSQFQQVW
MRKDPPVDEAYLYATHLLELAERQGVQVLNRPASLRAWNEKLGALQFPELMAPTLVASDVELLRQFAATHGEVVLKPLGG
RAGQGVIRSSGEAPGLGALLELVTQQQQLPVMIQAFLPQVSEGDKRILLVNGEPLGAVNRKPKAGEFRSNLAVGGQPEAT
DLSPRERQICDVLRPALIEAGLFFVGIDVIAGHLSEINVTSPTGIREVEQLGKIPLADLTQQRLLQG
>Mature_306_residues
GRQLFLIDPIGRLRPEKDSSVALMQAGQRAGEEIWAAQPSDLAAGEGGPRVLAAPITAEPWYSAGEARWQPLSQFQQVWM
RKDPPVDEAYLYATHLLELAERQGVQVLNRPASLRAWNEKLGALQFPELMAPTLVASDVELLRQFAATHGEVVLKPLGGR
AGQGVIRSSGEAPGLGALLELVTQQQQLPVMIQAFLPQVSEGDKRILLVNGEPLGAVNRKPKAGEFRSNLAVGGQPEATD
LSPRERQICDVLRPALIEAGLFFVGIDVIAGHLSEINVTSPTGIREVEQLGKIPLADLTQQRLLQG

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=289, Percent_Identity=39.7923875432526, Blast_Score=211, Evalue=6e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 33241; Mature: 33109

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRQLFLIDPIGRLRPEKDSSVALMQAGQRAGEEIWAAQPSDLAAGEGGPRVLAAPITAE
CCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCC
PWYSAGEARWQPLSQFQQVWMRKDPPVDEAYLYATHLLELAERQGVQVLNRPASLRAWNE
CCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHH
KLGALQFPELMAPTLVASDVELLRQFAATHGEVVLKPLGGRAGQGVIRSSGEAPGLGALL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCCHHHHH
ELVTQQQQLPVMIQAFLPQVSEGDKRILLVNGEPLGAVNRKPKAGEFRSNLAVGGQPEAT
HHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCC
DLSPRERQICDVLRPALIEAGLFFVGIDVIAGHLSEINVTSPTGIREVEQLGKIPLADLT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHH
QQRLLQG
HHHHHCC
>Mature Secondary Structure 
GRQLFLIDPIGRLRPEKDSSVALMQAGQRAGEEIWAAQPSDLAAGEGGPRVLAAPITAE
CCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCC
PWYSAGEARWQPLSQFQQVWMRKDPPVDEAYLYATHLLELAERQGVQVLNRPASLRAWNE
CCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHH
KLGALQFPELMAPTLVASDVELLRQFAATHGEVVLKPLGGRAGQGVIRSSGEAPGLGALL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCCHHHHH
ELVTQQQQLPVMIQAFLPQVSEGDKRILLVNGEPLGAVNRKPKAGEFRSNLAVGGQPEAT
HHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCC
DLSPRERQICDVLRPALIEAGLFFVGIDVIAGHLSEINVTSPTGIREVEQLGKIPLADLT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHH
QQRLLQG
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]