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The map label for this gene is trmE

Identifier: 148243337

GI number: 148243337

Start: 1945837

End: 1947198

Strand: Direct

Name: trmE

Synonym: SynRCC307_2238

Alternate gene names: 148243337

Gene position: 1945837-1947198 (Clockwise)

Preceding gene: 148243336

Following gene: 148243339

Centisome position: 87.46

GC content: 65.57

Gene sequence:

>1362_bases
ATGAGCCTGGCGGCGCGCGACGACACCATCGCAGCGATCGCCACGGCAGTCGCCGCTGGCGCCGGCAGCGTGGCCATCGT
GCGGATCAGCGGCCCGCAAGCCGAAGCCATTGGCCGCCAACTGTTCCAACCGGCTGGCAAGCAGAGCTGGGAGAGCCATC
GGGTGCTCTACGGCCACGTCAATGATCCAGCCAATGGCGATGTGGTGGATGAGGTGCTGCTGCTGCTCATGCGCGCGCCG
CGCAGCTTCACCCGCGAAACAGTGGTGGAGTTCCACGGCCATGGCGGGCTGGTGGCGGTGCAACGGCTGTTAGAGCTGGT
CTTAGCGGCAGGCGCCCGCCGCGCCCTGCCTGGCGAATTCAGCCAGCGGGCCTTTCTCAATGGGCGCCTTGATCTCACCC
GCGCTGAGGCCATCAGCGAATTGGTGAGTGCCAGGAGCCGCCGGGCCGCTGAGCTGGCGATGGCCGGGCTGGATGGGGGG
CTGCAACAACGGATCGAGGCACTGCGCGATCAATTGCTCGATCAGCTCTGCGAGCTCGAAGCACGCGTGGACTTCGAAGA
GGACCTGCCCAGCCTCGATGGAGCGGCGGTTTGCACGGCCTTAAGAGACGTTCAGCAGGCGTTGGATCAACTGGTGCTCG
ACGGGCAGCAAGCCCAGTTGCTACGCGATGGCTTGCGGGTGGCGATTATTGGCCGGCCCAATGTGGGTAAAAGCAGCCTG
CTCAATGCCCTCAGCGGCCACGAGCGCGCCATCGTCACCGATCTGCCGGGCACCACGCGCGACCTGTTGGACTACGACCT
GGTGCTCCAAGGGGTGCCGATCACCTTGCTCGACACCGCCGGCATTCGCTCCACCGCCGATCGCGTTGAGCAACTCGGCA
TTGAACGCAGCCGCGCTGCTTTTGCCAGCGCTGATGCGGTGGTGCTGCTCTATGACCTCAGCCGTGGCTGGAGCCCTGAC
GACAGCGCGCTGCGCAACGAGGTGCCAGATGGCACGCCCCTGCTGGTGGTGGGCAACAAATCGGATCTCGCTGCTGAACC
TGCAACGACGCAAGGACTGGCGATCAGCGCCCGCAGCGGACTGGGATTGGCCGAGCTGAGCAGCGCACTGCTCAAGCTCT
GCGGCCTCAGCGGCGAGGGGCAGGGCTTGCTGCTGGCGCTCAATCAACGCCAATGCGATCTAGCCGCCGCGGCCTCAGCC
GCCTTAGGGCGCAGTCAGCAGGCAGCGCGCGATGGCTTGCCCTGGGATTTTTGGACCATTGATCTGCGCGAAGCGATTCG
CGCACTCGGCGAGATCACCGGCGCTGAGATCACCGAAGCCGTGCTGGATCGCGTCTTCTCGCGCTTCTGCATCGGCAAGT
AG

Upstream 100 bases:

>100_bases
CCTCTGAGCGATCCCAAGGTCTTGTGGCTGTGATTTCGCTGGTGGTCGCCGATGTGCAACTGATCGTCTACTGGTCGCGA
GCCCTGAGGCGGCAACGTCC

Downstream 100 bases:

>100_bases
CCCGTTAGGGCTCGCTGGCACCGGCCGCCTGACGGGGCAGCACGCGGGCCAACAGCAACAAAGCGGTGACCACGGCATAG
GCCAGCGCCCATGGCGCCCC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSLAARDDTIAAIATAVAAGAGSVAIVRISGPQAEAIGRQLFQPAGKQSWESHRVLYGHVNDPANGDVVDEVLLLLMRAP
RSFTRETVVEFHGHGGLVAVQRLLELVLAAGARRALPGEFSQRAFLNGRLDLTRAEAISELVSARSRRAAELAMAGLDGG
LQQRIEALRDQLLDQLCELEARVDFEEDLPSLDGAAVCTALRDVQQALDQLVLDGQQAQLLRDGLRVAIIGRPNVGKSSL
LNALSGHERAIVTDLPGTTRDLLDYDLVLQGVPITLLDTAGIRSTADRVEQLGIERSRAAFASADAVVLLYDLSRGWSPD
DSALRNEVPDGTPLLVVGNKSDLAAEPATTQGLAISARSGLGLAELSSALLKLCGLSGEGQGLLLALNQRQCDLAAAASA
ALGRSQQAARDGLPWDFWTIDLREAIRALGEITGAEITEAVLDRVFSRFCIGK

Sequences:

>Translated_453_residues
MSLAARDDTIAAIATAVAAGAGSVAIVRISGPQAEAIGRQLFQPAGKQSWESHRVLYGHVNDPANGDVVDEVLLLLMRAP
RSFTRETVVEFHGHGGLVAVQRLLELVLAAGARRALPGEFSQRAFLNGRLDLTRAEAISELVSARSRRAAELAMAGLDGG
LQQRIEALRDQLLDQLCELEARVDFEEDLPSLDGAAVCTALRDVQQALDQLVLDGQQAQLLRDGLRVAIIGRPNVGKSSL
LNALSGHERAIVTDLPGTTRDLLDYDLVLQGVPITLLDTAGIRSTADRVEQLGIERSRAAFASADAVVLLYDLSRGWSPD
DSALRNEVPDGTPLLVVGNKSDLAAEPATTQGLAISARSGLGLAELSSALLKLCGLSGEGQGLLLALNQRQCDLAAAASA
ALGRSQQAARDGLPWDFWTIDLREAIRALGEITGAEITEAVLDRVFSRFCIGK
>Mature_452_residues
SLAARDDTIAAIATAVAAGAGSVAIVRISGPQAEAIGRQLFQPAGKQSWESHRVLYGHVNDPANGDVVDEVLLLLMRAPR
SFTRETVVEFHGHGGLVAVQRLLELVLAAGARRALPGEFSQRAFLNGRLDLTRAEAISELVSARSRRAAELAMAGLDGGL
QQRIEALRDQLLDQLCELEARVDFEEDLPSLDGAAVCTALRDVQQALDQLVLDGQQAQLLRDGLRVAIIGRPNVGKSSLL
NALSGHERAIVTDLPGTTRDLLDYDLVLQGVPITLLDTAGIRSTADRVEQLGIERSRAAFASADAVVLLYDLSRGWSPDD
SALRNEVPDGTPLLVVGNKSDLAAEPATTQGLAISARSGLGLAELSSALLKLCGLSGEGQGLLLALNQRQCDLAAAASAA
LGRSQQAARDGLPWDFWTIDLREAIRALGEITGAEITEAVLDRVFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=34.8936170212766, Blast_Score=181, Evalue=9e-46,
Organism=Homo sapiens, GI193082993, Length=460, Percent_Identity=35.4347826086957, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI193082988, Length=501, Percent_Identity=32.9341317365269, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI2367268, Length=461, Percent_Identity=38.6117136659436, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI87082120, Length=166, Percent_Identity=34.9397590361446, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=463, Percent_Identity=30.2375809935205, Blast_Score=179, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=510, Percent_Identity=29.4117647058824, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI45550813, Length=469, Percent_Identity=31.7697228144989, Blast_Score=188, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_SYNR3 (A5GW82)

Other databases:

- EMBL:   CT978603
- RefSeq:   YP_001228494.1
- ProteinModelPortal:   A5GW82
- SMR:   A5GW82
- STRING:   A5GW82
- GeneID:   5156628
- GenomeReviews:   CT978603_GR
- KEGG:   syr:SynRCC307_2238
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48074; Mature: 47943

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: BINDING 28-28 BINDING 90-90 BINDING 129-129 BINDING 453-453

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAARDDTIAAIATAVAAGAGSVAIVRISGPQAEAIGRQLFQPAGKQSWESHRVLYGHV
CCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEC
NDPANGDVVDEVLLLLMRAPRSFTRETVVEFHGHGGLVAVQRLLELVLAAGARRALPGEF
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCH
SQRAFLNGRLDLTRAEAISELVSARSRRAAELAMAGLDGGLQQRIEALRDQLLDQLCELE
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ARVDFEEDLPSLDGAAVCTALRDVQQALDQLVLDGQQAQLLRDGLRVAIIGRPNVGKSSL
HCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECCCCCHHHH
LNALSGHERAIVTDLPGTTRDLLDYDLVLQGVPITLLDTAGIRSTADRVEQLGIERSRAA
HHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHHHHHH
FASADAVVLLYDLSRGWSPDDSALRNEVPDGTPLLVVGNKSDLAAEPATTQGLAISARSG
HHCCCEEEEEEECCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCC
LGLAELSSALLKLCGLSGEGQGLLLALNQRQCDLAAAASAALGRSQQAARDGLPWDFWTI
CCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCEEEE
DLREAIRALGEITGAEITEAVLDRVFSRFCIGK
EHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLAARDDTIAAIATAVAAGAGSVAIVRISGPQAEAIGRQLFQPAGKQSWESHRVLYGHV
CCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEC
NDPANGDVVDEVLLLLMRAPRSFTRETVVEFHGHGGLVAVQRLLELVLAAGARRALPGEF
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCH
SQRAFLNGRLDLTRAEAISELVSARSRRAAELAMAGLDGGLQQRIEALRDQLLDQLCELE
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ARVDFEEDLPSLDGAAVCTALRDVQQALDQLVLDGQQAQLLRDGLRVAIIGRPNVGKSSL
HCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECCCCCHHHH
LNALSGHERAIVTDLPGTTRDLLDYDLVLQGVPITLLDTAGIRSTADRVEQLGIERSRAA
HHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHHHHHH
FASADAVVLLYDLSRGWSPDDSALRNEVPDGTPLLVVGNKSDLAAEPATTQGLAISARSG
HHCCCEEEEEEECCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCC
LGLAELSSALLKLCGLSGEGQGLLLALNQRQCDLAAAASAALGRSQQAARDGLPWDFWTI
CCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCEEEE
DLREAIRALGEITGAEITEAVLDRVFSRFCIGK
EHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA