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The map label for this gene is trmB [H]

Identifier: 148243315

GI number: 148243315

Start: 1922902

End: 1923612

Strand: Direct

Name: trmB [H]

Synonym: SynRCC307_2216

Alternate gene names: 148243315

Gene position: 1922902-1923612 (Clockwise)

Preceding gene: 148243314

Following gene: 148243316

Centisome position: 86.43

GC content: 60.48

Gene sequence:

>711_bases
ATGCTGGGCCCTTGTGGTTCCAACCCAGTCCTGAGGTCCTTGCGGCAGCACGTCAATCCACTCAGCAGCTTCTTCCAGCA
GGAGCGTCCATTACCCGGCTTAGCTGAGCTCTTCGCCAGACCAGAGCAACCGCTCCACCTCGACATCGGCTGCGCCCGCG
GCAAATTCCTGCTTCAGATGGCGAGCCAGACCCCGCAGCGCAATCACCTGGGCCTGGAGATCCGCCATCCCTTGGTGAAG
CAAGCGGAAGCCGAGCGCCTGCAACTGGAGCTCACCAACCTGCACTTTCTGTTCTGCAACGCCAACATCAGCCTGCGTCA
TTGGTTGGAGCAGCTGCCCATTGGGCGATTGGAGCTGGTCACCATTCAGTACCCCGACCCCTGGTTTAAAAAGCGGCATC
AAAAACGGCGCATGGTGCAGCCCGAGCTCATCAGCTGGGTGGCTGCAGCGATGGCACCAGGGGCTCAGCTCTTTTTGCAG
AGCGATGTGCCCGATGTGATCAACCAGATGCTGGAGGCCACAACCAGCGGTCCCTGGTTTGAACCCGCCGATGGGGGCCA
ATGGCTCAGCGACAACCCCTTGCAGCAACCCACTGAACGCGAGCTGTATGTGCTGAAACAGGGCCTGCCGGTTTACAGAC
GGCTCTTTGCTCGAACCACAGCAGCCGTTCAAGCCCATAATCCGTGCGCCGCTGACGCTGCGACGCCATGA

Upstream 100 bases:

>100_bases
TCTGCCGCAAGCACTTCCCAGAGCTGCCCATCAGCGGCTTGTTCTGCAATGGTGAAATCGGCCCAGTGGACGGCAGCACC
CAGCTGCACGGCTACACCGC

Downstream 100 bases:

>100_bases
GTCCCTCTCCAGCCGCCCCTTGGCTGCTGTGCTGGCAGGGACTGCTGGCCAGCGAGGCAACGAGTCGCCTGCGCAAGCTG
CTGCCCCAACTGGCAGGCGG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MLGPCGSNPVLRSLRQHVNPLSSFFQQERPLPGLAELFARPEQPLHLDIGCARGKFLLQMASQTPQRNHLGLEIRHPLVK
QAEAERLQLELTNLHFLFCNANISLRHWLEQLPIGRLELVTIQYPDPWFKKRHQKRRMVQPELISWVAAAMAPGAQLFLQ
SDVPDVINQMLEATTSGPWFEPADGGQWLSDNPLQQPTERELYVLKQGLPVYRRLFARTTAAVQAHNPCAADAATP

Sequences:

>Translated_236_residues
MLGPCGSNPVLRSLRQHVNPLSSFFQQERPLPGLAELFARPEQPLHLDIGCARGKFLLQMASQTPQRNHLGLEIRHPLVK
QAEAERLQLELTNLHFLFCNANISLRHWLEQLPIGRLELVTIQYPDPWFKKRHQKRRMVQPELISWVAAAMAPGAQLFLQ
SDVPDVINQMLEATTSGPWFEPADGGQWLSDNPLQQPTERELYVLKQGLPVYRRLFARTTAAVQAHNPCAADAATP
>Mature_236_residues
MLGPCGSNPVLRSLRQHVNPLSSFFQQERPLPGLAELFARPEQPLHLDIGCARGKFLLQMASQTPQRNHLGLEIRHPLVK
QAEAERLQLELTNLHFLFCNANISLRHWLEQLPIGRLELVTIQYPDPWFKKRHQKRRMVQPELISWVAAAMAPGAQLFLQ
SDVPDVINQMLEATTSGPWFEPADGGQWLSDNPLQQPTERELYVLKQGLPVYRRLFARTTAAVQAHNPCAADAATP

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=190, Percent_Identity=30, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26727; Mature: 26727

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGPCGSNPVLRSLRQHVNPLSSFFQQERPLPGLAELFARPEQPLHLDIGCARGKFLLQM
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCHHHHHHH
ASQTPQRNHLGLEIRHPLVKQAEAERLQLELTNLHFLFCNANISLRHWLEQLPIGRLELV
HHCCCCCCCCCEEECCHHHHHHHHHHHEEEEEEEEEEEEECCCHHHHHHHHCCCCCEEEE
TIQYPDPWFKKRHQKRRMVQPELISWVAAAMAPGAQLFLQSDVPDVINQMLEATTSGPWF
EEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHCCCCCC
EPADGGQWLSDNPLQQPTERELYVLKQGLPVYRRLFARTTAAVQAHNPCAADAATP
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLGPCGSNPVLRSLRQHVNPLSSFFQQERPLPGLAELFARPEQPLHLDIGCARGKFLLQM
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCHHHHHHH
ASQTPQRNHLGLEIRHPLVKQAEAERLQLELTNLHFLFCNANISLRHWLEQLPIGRLELV
HHCCCCCCCCCEEECCHHHHHHHHHHHEEEEEEEEEEEEECCCHHHHHHHHCCCCCEEEE
TIQYPDPWFKKRHQKRRMVQPELISWVAAAMAPGAQLFLQSDVPDVINQMLEATTSGPWF
EEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHCCCCCC
EPADGGQWLSDNPLQQPTERELYVLKQGLPVYRRLFARTTAAVQAHNPCAADAATP
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA