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The map label for this gene is nusA [H]

Identifier: 148243067

GI number: 148243067

Start: 1712136

End: 1713551

Strand: Direct

Name: nusA [H]

Synonym: SynRCC307_1968

Alternate gene names: 148243067

Gene position: 1712136-1713551 (Clockwise)

Preceding gene: 148243066

Following gene: 148243068

Centisome position: 76.95

GC content: 61.51

Gene sequence:

>1416_bases
ATGGCACTCGTGATGCTCCCGGGCCTCTCGGCCCTCATTGAAGACATCAGCGAGGAGAAGAAGCTGCCCGCCAACGTGGT
GGAGGCCGCCCTGCGCGAAGCCCTTCTCAAGGGCTATGAGCGCTACCGCCGCACGCTCTACATCGGCATTAGCGAAGACC
CGTTCGACGAAGAATATTTCAGCAACTTCGACGTGGGCCTCGACCTCGATGAAGAGGGTTACCGGGTTCTGGCCTCAAAA
ATCATTGTTGAAGAGGTGGAAAGCGAGGACCATCAAATTGGTCTGGCGGACGTGCAGCAGGTGACCGACGACGCCCAACT
GGGCGACACGGTGGTGCTGGATGTGACCCCCGAAAAAGATGATTTCGGGCGCATGGCAGCAGCGACCACCAAGCAGGTGC
TGGCCCAGAAATTGCGGGAGCATCAGCGCCGCATGATCCAGGAGGAATTCGCCGACCTAGAAGATCCGGTTCTGACCGCT
CGCGTCATCCGCTTTGAGCGCCAGAGCGTGATCATGGCTGTGAGTTCCGGACTGGGCCGCCCGGAAGTGGAAGCTGAACT
GCCACGGCGCGACCAGCTCCCCAACGACAACTACAGGGCCAACGCCACCTTCAAGGTCTTCCTTAAAGAGGTGTCTGAAA
TTGCCCGCCGCGGCCCCCAGCTGTTCATCAGCCGGGCTAACGCCGGGTTGGTTGTTTATTTGTTCGAAAACGAAGTTCCC
GAGATCCAGGAAGGGTCAGTGCGGATCGTGGCCGTTGCCCGTGAAGCCAACCCACCTTCGAGAGCCGTTGGACCTCGCAC
CAAGGTGGCGGTCGACAGTGTTGAACGGGAAGTTGACCCGGTTGGCGCCTGCATTGGCGCGCGGGGCTCCCGGATTCAAC
AGGTGGTCAACGAACTGCGCGGCGAGAAAATTGACGTGATCCGTTGGTCGCCTGACCCCGGCCAGTACCTCGCTAACTCC
CTCAGCCCAGCACGGGTGGACATGGTGCGTCTGGTGGACCCAGAAGGGCAGCACGCCCACGTGTTGGTTCCGCCCGACCA
GCTGAGCTTGGCCATCGGCCGCGAGGGCCAAAACGTGCGCCTGGCAGCACGCTTGACCGGCTGGAAAATCGATATCAAGA
ACAGCGAGGAATACGACCAGGCCTCCGAGGACGAGCAGGTTGCTGAGCTGATCGAGCACCGCCGTGAGGAGGAAGCACTC
CAAGCGGAAGCCGAAGCCCGCCTCGAAGCCGAACAAGCTGCCCGAGCCGAGGAAGATGCTCGCCTCAGGGAGCTCTATCC
AATCGAGGAAGACGAGCTCGAGGACTACGGCGAGGAACAGCAACCCATCGCAGAGGCCGAAGTCGAAGCCGAACCAGAGC
CTGCCGCCGAAGCCAGCAGCGAAGAGCCCGTGGCCAGCGAGGACGAGGCCAGGTGA

Upstream 100 bases:

>100_bases
TCAGCGGATCCCACGCAGCGATGTGCTCGAGGTTCGTCTGACCTCACCCAGCGCCTAACGGCACGCCCACCTTTCTGTTC
ACTGCTTCAACGGATCACCC

Downstream 100 bases:

>100_bases
GTGCTCAGCCCACCCTGCGCCGCTGCGTGAGTTGCCGGGAACTGTTCGACCGTCGTCAGTTGCTGCGCATCATCCGGCTT
GCAGGGGGTGCCGGCGTTGT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MALVMLPGLSALIEDISEEKKLPANVVEAALREALLKGYERYRRTLYIGISEDPFDEEYFSNFDVGLDLDEEGYRVLASK
IIVEEVESEDHQIGLADVQQVTDDAQLGDTVVLDVTPEKDDFGRMAAATTKQVLAQKLREHQRRMIQEEFADLEDPVLTA
RVIRFERQSVIMAVSSGLGRPEVEAELPRRDQLPNDNYRANATFKVFLKEVSEIARRGPQLFISRANAGLVVYLFENEVP
EIQEGSVRIVAVAREANPPSRAVGPRTKVAVDSVEREVDPVGACIGARGSRIQQVVNELRGEKIDVIRWSPDPGQYLANS
LSPARVDMVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDIKNSEEYDQASEDEQVAELIEHRREEEAL
QAEAEARLEAEQAARAEEDARLRELYPIEEDELEDYGEEQQPIAEAEVEAEPEPAAEASSEEPVASEDEAR

Sequences:

>Translated_471_residues
MALVMLPGLSALIEDISEEKKLPANVVEAALREALLKGYERYRRTLYIGISEDPFDEEYFSNFDVGLDLDEEGYRVLASK
IIVEEVESEDHQIGLADVQQVTDDAQLGDTVVLDVTPEKDDFGRMAAATTKQVLAQKLREHQRRMIQEEFADLEDPVLTA
RVIRFERQSVIMAVSSGLGRPEVEAELPRRDQLPNDNYRANATFKVFLKEVSEIARRGPQLFISRANAGLVVYLFENEVP
EIQEGSVRIVAVAREANPPSRAVGPRTKVAVDSVEREVDPVGACIGARGSRIQQVVNELRGEKIDVIRWSPDPGQYLANS
LSPARVDMVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDIKNSEEYDQASEDEQVAELIEHRREEEAL
QAEAEARLEAEQAARAEEDARLRELYPIEEDELEDYGEEQQPIAEAEVEAEPEPAAEASSEEPVASEDEAR
>Mature_470_residues
ALVMLPGLSALIEDISEEKKLPANVVEAALREALLKGYERYRRTLYIGISEDPFDEEYFSNFDVGLDLDEEGYRVLASKI
IVEEVESEDHQIGLADVQQVTDDAQLGDTVVLDVTPEKDDFGRMAAATTKQVLAQKLREHQRRMIQEEFADLEDPVLTAR
VIRFERQSVIMAVSSGLGRPEVEAELPRRDQLPNDNYRANATFKVFLKEVSEIARRGPQLFISRANAGLVVYLFENEVPE
IQEGSVRIVAVAREANPPSRAVGPRTKVAVDSVEREVDPVGACIGARGSRIQQVVNELRGEKIDVIRWSPDPGQYLANSL
SPARVDMVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDIKNSEEYDQASEDEQVAELIEHRREEEALQ
AEAEARLEAEQAARAEEDARLRELYPIEEDELEDYGEEQQPIAEAEVEAEPEPAAEASSEEPVASEDEAR

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=364, Percent_Identity=34.0659340659341, Blast_Score=184, Evalue=9e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 52637; Mature: 52506

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVMLPGLSALIEDISEEKKLPANVVEAALREALLKGYERYRRTLYIGISEDPFDEEYF
CEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHH
SNFDVGLDLDEEGYRVLASKIIVEEVESEDHQIGLADVQQVTDDAQLGDTVVLDVTPEKD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHCHHHCCCEEEEEECCCCC
DFGRMAAATTKQVLAQKLREHQRRMIQEEFADLEDPVLTARVIRFERQSVIMAVSSGLGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PEVEAELPRRDQLPNDNYRANATFKVFLKEVSEIARRGPQLFISRANAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCC
EIQEGSVRIVAVAREANPPSRAVGPRTKVAVDSVEREVDPVGACIGARGSRIQQVVNELR
CCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GEKIDVIRWSPDPGQYLANSLSPARVDMVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCHHHHHHHCCCCHHEEEEEEECCCCCEEEEEECCHHEEEEECCCCCCEE
LAARLTGWKIDIKNSEEYDQASEDEQVAELIEHRREEEALQAEAEARLEAEQAARAEEDA
EEEEEECEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RLRELYPIEEDELEDYGEEQQPIAEAEVEAEPEPAAEASSEEPVASEDEAR
HHHHCCCCCHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALVMLPGLSALIEDISEEKKLPANVVEAALREALLKGYERYRRTLYIGISEDPFDEEYF
EEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHH
SNFDVGLDLDEEGYRVLASKIIVEEVESEDHQIGLADVQQVTDDAQLGDTVVLDVTPEKD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHCHHHCCCEEEEEECCCCC
DFGRMAAATTKQVLAQKLREHQRRMIQEEFADLEDPVLTARVIRFERQSVIMAVSSGLGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PEVEAELPRRDQLPNDNYRANATFKVFLKEVSEIARRGPQLFISRANAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCC
EIQEGSVRIVAVAREANPPSRAVGPRTKVAVDSVEREVDPVGACIGARGSRIQQVVNELR
CCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GEKIDVIRWSPDPGQYLANSLSPARVDMVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCHHHHHHHCCCCHHEEEEEEECCCCCEEEEEECCHHEEEEECCCCCCEE
LAARLTGWKIDIKNSEEYDQASEDEQVAELIEHRREEEALQAEAEARLEAEQAARAEEDA
EEEEEECEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RLRELYPIEEDELEDYGEEQQPIAEAEVEAEPEPAAEASSEEPVASEDEAR
HHHHCCCCCHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]