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The map label for this gene is truB [H]

Identifier: 148242930

GI number: 148242930

Start: 1593227

End: 1594183

Strand: Direct

Name: truB [H]

Synonym: SynRCC307_1831

Alternate gene names: 148242930

Gene position: 1593227-1594183 (Clockwise)

Preceding gene: 148242927

Following gene: 148242931

Centisome position: 71.61

GC content: 67.29

Gene sequence:

>957_bases
ATGGACGTTGCCGTTCGTGAGCCTTCAACTTTGGCAAATGGCAGCATCACCCCAGTGATTGCCCTGCCCGCTGTGACCGG
CCCCCTGTGCGGCTTTTTGGTAATCGACAAGCCAGCCGGCTGCACATCCCACGACTGCGTCTCGAGGGCCAGGCGAGTCC
TGGGCACCCGCAAGGTGGGCCATGGCGGGACCCTCGATCCAGCCGTCACCGGCGTGTTGCCCTTGGCAGTGGGGCCTGCA
ACGCGGCTGCTGCCCTACCTCGATGGCGGCAAGGAATACCTCGGCACCATCCAGCTGGGCCTACGCACCAACAGTGACGA
CCTCAGTGGGGAGGTGCTGGAGCAACAAGACTGGCCGAGCTTGAGCCAAGCAGAGCTCGACAGGCAGCTGCAGCCCTTCC
GCGGCCTGATCCAACAACGGCCGCCAGCGGTGAGTGCGGTGCATGTCGATGGCGAACGGGCCCACACCCGGGCCCGGCGC
GGCGAACAGCTGCTGCTGGCCAGCCGAGCGGTGACGATCGAGCAACTCGCTCTACTGCGCTGGGATCCCAGCACGGGCCA
ACTGGACATCAAGATGCGCTGCTCGGCGGGCACCTATGTGCGCTCACTGGCCCGAGACCTGGGCGATCAGCTGGGCTGCG
GCGGCGCCTTAGCCCAGCTGCGGCGAACCGCTGCCTTGGGCTTCACGCTGGAGGCCAGCCTGCCGCTGGAGGCCCTGGGC
CCAGAGACCGCGCTTAGCAATCCGCTCACAGCCCTGGCGCACCTGCCTCAGCAACGGCTCAGTGAGGAGCAGTGGCTGGC
TTGGACCCGCGGCCAGCGCTTGCCACTGGAGGGCCCAGAGCAGCCCGAGGAGAGCGCCTTGGTGATGCTGCGGCCCGATG
GCAGCCTGGCGGGCATGGCCCGCACCCGAAGCGATGGGTTGCTGCAGCCGAAACTGGTTTTTGATGCCGCCGGATGA

Upstream 100 bases:

>100_bases
AAGTTGCTCAGCGCTGAGCTGCAGAAAGCTGGCCAACTGCTGCCGCAAAGCGAAGCCATCGCGGCCAAACGCCGCAATCA
CTTCAGCCTGCGCAGATGTC

Downstream 100 bases:

>100_bases
TCACTCAGCCCATGGCCCTTGGATCCAGGCTGCTGCTGGCGGTGTTGGTGCTGCTGAGCGGCTGTCAGCTGGAAAGCTTC
GCTGAGCGCCAGGCGCGGCA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MDVAVREPSTLANGSITPVIALPAVTGPLCGFLVIDKPAGCTSHDCVSRARRVLGTRKVGHGGTLDPAVTGVLPLAVGPA
TRLLPYLDGGKEYLGTIQLGLRTNSDDLSGEVLEQQDWPSLSQAELDRQLQPFRGLIQQRPPAVSAVHVDGERAHTRARR
GEQLLLASRAVTIEQLALLRWDPSTGQLDIKMRCSAGTYVRSLARDLGDQLGCGGALAQLRRTAALGFTLEASLPLEALG
PETALSNPLTALAHLPQQRLSEEQWLAWTRGQRLPLEGPEQPEESALVMLRPDGSLAGMARTRSDGLLQPKLVFDAAG

Sequences:

>Translated_318_residues
MDVAVREPSTLANGSITPVIALPAVTGPLCGFLVIDKPAGCTSHDCVSRARRVLGTRKVGHGGTLDPAVTGVLPLAVGPA
TRLLPYLDGGKEYLGTIQLGLRTNSDDLSGEVLEQQDWPSLSQAELDRQLQPFRGLIQQRPPAVSAVHVDGERAHTRARR
GEQLLLASRAVTIEQLALLRWDPSTGQLDIKMRCSAGTYVRSLARDLGDQLGCGGALAQLRRTAALGFTLEASLPLEALG
PETALSNPLTALAHLPQQRLSEEQWLAWTRGQRLPLEGPEQPEESALVMLRPDGSLAGMARTRSDGLLQPKLVFDAAG
>Mature_318_residues
MDVAVREPSTLANGSITPVIALPAVTGPLCGFLVIDKPAGCTSHDCVSRARRVLGTRKVGHGGTLDPAVTGVLPLAVGPA
TRLLPYLDGGKEYLGTIQLGLRTNSDDLSGEVLEQQDWPSLSQAELDRQLQPFRGLIQQRPPAVSAVHVDGERAHTRARR
GEQLLLASRAVTIEQLALLRWDPSTGQLDIKMRCSAGTYVRSLARDLGDQLGCGGALAQLRRTAALGFTLEASLPLEALG
PETALSNPLTALAHLPQQRLSEEQWLAWTRGQRLPLEGPEQPEESALVMLRPDGSLAGMARTRSDGLLQPKLVFDAAG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=221, Percent_Identity=34.841628959276, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI215599015, Length=194, Percent_Identity=34.5360824742268, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4503337, Length=194, Percent_Identity=34.5360824742268, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI2367200, Length=212, Percent_Identity=35.377358490566, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17553978, Length=194, Percent_Identity=34.020618556701, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=221, Percent_Identity=33.9366515837104, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=142, Percent_Identity=35.9154929577465, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364189, Length=194, Percent_Identity=33.5051546391753, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364187, Length=194, Percent_Identity=33.5051546391753, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364185, Length=194, Percent_Identity=33.5051546391753, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364183, Length=194, Percent_Identity=33.5051546391753, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI62471759, Length=194, Percent_Identity=33.5051546391753, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17975520, Length=194, Percent_Identity=33.5051546391753, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015225
- InterPro:   IPR014780 [H]

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33953; Mature: 33953

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVAVREPSTLANGSITPVIALPAVTGPLCGFLVIDKPAGCTSHDCVSRARRVLGTRKVG
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCC
HGGTLDPAVTGVLPLAVGPATRLLPYLDGGKEYLGTIQLGLRTNSDDLSGEVLEQQDWPS
CCCCCCHHHHHHHHHCCCCHHHHHHCCCCCHHEEEEEEEEEECCCCCCCCHHHCCCCCCC
LSQAELDRQLQPFRGLIQQRPPAVSAVHVDGERAHTRARRGEQLLLASRAVTIEQLALLR
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCEEEHHHHHHHHHHHHHEE
WDPSTGQLDIKMRCSAGTYVRSLARDLGDQLGCGGALAQLRRTAALGFTLEASLPLEALG
ECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEECCCCHHHCC
PETALSNPLTALAHLPQQRLSEEQWLAWTRGQRLPLEGPEQPEESALVMLRPDGSLAGMA
CCHHHCCHHHHHHHCCHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHH
RTRSDGLLQPKLVFDAAG
CCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MDVAVREPSTLANGSITPVIALPAVTGPLCGFLVIDKPAGCTSHDCVSRARRVLGTRKVG
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCC
HGGTLDPAVTGVLPLAVGPATRLLPYLDGGKEYLGTIQLGLRTNSDDLSGEVLEQQDWPS
CCCCCCHHHHHHHHHCCCCHHHHHHCCCCCHHEEEEEEEEEECCCCCCCCHHHCCCCCCC
LSQAELDRQLQPFRGLIQQRPPAVSAVHVDGERAHTRARRGEQLLLASRAVTIEQLALLR
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCEEEHHHHHHHHHHHHHEE
WDPSTGQLDIKMRCSAGTYVRSLARDLGDQLGCGGALAQLRRTAALGFTLEASLPLEALG
ECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEECCCCHHHCC
PETALSNPLTALAHLPQQRLSEEQWLAWTRGQRLPLEGPEQPEESALVMLRPDGSLAGMA
CCHHHCCHHHHHHHCCHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHH
RTRSDGLLQPKLVFDAAG
CCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]