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The map label for this gene is xthA [H]

Identifier: 148242745

GI number: 148242745

Start: 1441125

End: 1441952

Strand: Direct

Name: xthA [H]

Synonym: SynRCC307_1646

Alternate gene names: 148242745

Gene position: 1441125-1441952 (Clockwise)

Preceding gene: 148242743

Following gene: 148242747

Centisome position: 64.77

GC content: 62.56

Gene sequence:

>828_bases
GTGCGGATCGCCACCTGGAATGTCAATTCGATCCGCAGCCGGCAAAGCCATCTGCTGCGCTGGCTGGAGCAGGCCAAGCC
CGATGTGCTGGGGCTGCAGGAAACCAAGGTGGAAGACGCGCAATTTCCTGCGGCAGCCATGGAAGAAGCCGGCTACCGCA
CCGTGATCAGCGGCCAGAAGAGTTACAACGGCGTGGCACTCCTCAGCCGCGAACCGCTCGAAGAGGTGCGGATCGGTTTT
GACGCCCTGCTGCCAGGCGATGAGGCCGCCATCGAGCTGAGCCAGCAGAAGCGGGTGATCAGCGCCTGGGTGGATGGCGT
GCGGGTGCTCAATCTCTATGTGCCCAACGGATCGGAGGTGGGCTCAGAGAAATACGCCTACAAACTGGCCTGGCTCGATT
GCCTGCGCCGTTACCTCGACGTGCAACGCGAGCAAGGGGAACCCTTTTGCATGCTGGGCGATTTCAACATCGGCCCGGAA
GCCCGCGACTTGCACAACCCGGAGCGCTCCACAGGCGGAATCATGGCCAGTGATGCCGAGCGGGAGGCGCTCAAGGCCTG
CCTCGGGGATGACCTGCGCGATGCCTTCCGCATGTTTGAGCAAGGCAGCGGCCACTGGAGCTGGTGGGACTACCGCTCAG
GCGCTTGGAACGGCGATCGCGGCTGGCGCATCGATCACCTTTATCTCGATGAGGAGCTGCAGCAGCGGGCCTTGGCCTGT
GAGATCCACAAAAGCGAACGGGGCCTCGAAAAGCCCAGCGATCACGCCCCGGTGAGTGTGGATCTCGCTGATGAAGCTGC
GGAAGAAGAAGACGAAGACCTCTGGTGA

Upstream 100 bases:

>100_bases
CGATCGCCGCTGGTGCCAGCAGCAGGGCCATGGCGCAGGCCAACAAAGTGCGTGTCATGGCTTCAATCGTTTAATGGGCA
GATTTTAAGAGGGGATCAGC

Downstream 100 bases:

>100_bases
GTCACACCACCTCAACACCCTTGGGCAGCGGAATCTGCTGAGCGCGGCGGCTGGATTCACCGCAGCTCACCGGGGTGGGG
AAGAGCTTGCCGGGATTGGC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRIATWNVNSIRSRQSHLLRWLEQAKPDVLGLQETKVEDAQFPAAAMEEAGYRTVISGQKSYNGVALLSREPLEEVRIGF
DALLPGDEAAIELSQQKRVISAWVDGVRVLNLYVPNGSEVGSEKYAYKLAWLDCLRRYLDVQREQGEPFCMLGDFNIGPE
ARDLHNPERSTGGIMASDAEREALKACLGDDLRDAFRMFEQGSGHWSWWDYRSGAWNGDRGWRIDHLYLDEELQQRALAC
EIHKSERGLEKPSDHAPVSVDLADEAAEEEDEDLW

Sequences:

>Translated_275_residues
MRIATWNVNSIRSRQSHLLRWLEQAKPDVLGLQETKVEDAQFPAAAMEEAGYRTVISGQKSYNGVALLSREPLEEVRIGF
DALLPGDEAAIELSQQKRVISAWVDGVRVLNLYVPNGSEVGSEKYAYKLAWLDCLRRYLDVQREQGEPFCMLGDFNIGPE
ARDLHNPERSTGGIMASDAEREALKACLGDDLRDAFRMFEQGSGHWSWWDYRSGAWNGDRGWRIDHLYLDEELQQRALAC
EIHKSERGLEKPSDHAPVSVDLADEAAEEEDEDLW
>Mature_275_residues
MRIATWNVNSIRSRQSHLLRWLEQAKPDVLGLQETKVEDAQFPAAAMEEAGYRTVISGQKSYNGVALLSREPLEEVRIGF
DALLPGDEAAIELSQQKRVISAWVDGVRVLNLYVPNGSEVGSEKYAYKLAWLDCLRRYLDVQREQGEPFCMLGDFNIGPE
ARDLHNPERSTGGIMASDAEREALKACLGDDLRDAFRMFEQGSGHWSWWDYRSGAWNGDRGWRIDHLYLDEELQQRALAC
EIHKSERGLEKPSDHAPVSVDLADEAAEEEDEDLW

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=270, Percent_Identity=30.3703703703704, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI18375503, Length=270, Percent_Identity=30.3703703703704, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI18375501, Length=270, Percent_Identity=30.3703703703704, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1788046, Length=270, Percent_Identity=35.5555555555556, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71989536, Length=273, Percent_Identity=27.8388278388278, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221330655, Length=265, Percent_Identity=26.7924528301887, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17136678, Length=265, Percent_Identity=26.7924528301887, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31237; Mature: 31237

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATWNVNSIRSRQSHLLRWLEQAKPDVLGLQETKVEDAQFPAAAMEEAGYRTVISGQK
CEEEECCHHHHHHHHHHHHHHHHHCCCCEECCHHHCCCHHHCCHHHHHHCCCEEEECCCC
SYNGVALLSREPLEEVRIGFDALLPGDEAAIELSQQKRVISAWVDGVRVLNLYVPNGSEV
CCCCEEEECCCCHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCC
GSEKYAYKLAWLDCLRRYLDVQREQGEPFCMLGDFNIGPEARDLHNPERSTGGIMASDAE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCEEECCHH
REALKACLGDDLRDAFRMFEQGSGHWSWWDYRSGAWNGDRGWRIDHLYLDEELQQRALAC
HHHHHHHHCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHH
EIHKSERGLEKPSDHAPVSVDLADEAAEEEDEDLW
HHHHHHCCCCCCCCCCCEEEECCHHHCCCCCCCCC
>Mature Secondary Structure
MRIATWNVNSIRSRQSHLLRWLEQAKPDVLGLQETKVEDAQFPAAAMEEAGYRTVISGQK
CEEEECCHHHHHHHHHHHHHHHHHCCCCEECCHHHCCCHHHCCHHHHHHCCCEEEECCCC
SYNGVALLSREPLEEVRIGFDALLPGDEAAIELSQQKRVISAWVDGVRVLNLYVPNGSEV
CCCCEEEECCCCHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCC
GSEKYAYKLAWLDCLRRYLDVQREQGEPFCMLGDFNIGPEARDLHNPERSTGGIMASDAE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCEEECCHH
REALKACLGDDLRDAFRMFEQGSGHWSWWDYRSGAWNGDRGWRIDHLYLDEELQQRALAC
HHHHHHHHCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHH
EIHKSERGLEKPSDHAPVSVDLADEAAEEEDEDLW
HHHHHHCCCCCCCCCCCEEEECCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]