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The map label for this gene is proP [H]

Identifier: 148242608

GI number: 148242608

Start: 1341984

End: 1343507

Strand: Direct

Name: proP [H]

Synonym: SynRCC307_1509

Alternate gene names: 148242608

Gene position: 1341984-1343507 (Clockwise)

Preceding gene: 148242605

Following gene: 148242609

Centisome position: 60.32

GC content: 62.07

Gene sequence:

>1524_bases
TTGGATGCGCTCGATGGCCTGTTTGGCCCGCTGCTCGTCGCGGCAGCCCAGCAGCACCTGGGCACCTTTGCGGGCGAGCT
GCAAAGCAGCTTCATAACCAATTCCCGTGTTGGCGCCTGTGACAAGGGCGAGCCGACCGGCCTGATCAGCCATGGCATCA
GCGGTCCAACGTGCCATCGCTCTGCAGCAAACGTCGTTGTCTTAGCGAACCCTTGCAGGGTCCTGACTTCATTCCTAATA
GTGAGCGCAAGCAAGCAGGCCGCCATGGGAGCCCATCAGCCACCGCTCTGGCGGGTGATCTTGGCTGGCCTGATTGGCAA
CGTCATGGAGTGGTACGACTTCGCCCTCTACGGCTACTTCGTTGGCATCTTCGGCGATCAGTTTTTTCCCTCGAGTGATC
CCAACGCTTCGCTGATTGCCGCCTTTGGAGCCTTTGCCGCCGGCTTTCTGGTGCGACCCCTTGGAGGTGTGGTGCTTGGA
CGCATCGGCGATCGGGTTGGCCGCCGCCAAGCTCTCACCCTCTCAGTGATGGCCATGGCGCTAGCCACGGTCTCGATGGC
GATCCTGCCCACCTACCAGCAGGTGGGCCTTTGGGCCCCGATCGCCCTGGTGTTGCTTCGCATCCTCCAAGGACTCTCTG
CCGGCGGTGAATACACCACCTCCATCGTGTTTCTGGCTGAGCATGCTCCGGCCAATCGCCGCGGTCTGGTCACCATCTGG
GGACTCTGGGGATCGGTGCTGGGCATGCTGCTGGGCTCAGCGGCAGGGACGCTGCTCTCAGACAGCCTGAGCGCTGCGCA
AATGTCCAGCTGGGGCTGGCGTCTGCCCTTTGCCTTGGGTCTGTTGGTGGCCCTCACTGGCTTCGTCCTGCGGCGGGGCC
TGAACAGCGAAACGCCACCGGCGGCTAGTGCCCAGCCAGTGCGGGCCTTGGCTCGCCACAGCGGCGCTGTGCTGCGAGTT
CTGCTGCTCAATGTCGCCAGCAGCGTTGCCTTCTACACGGCCTTCGTCTACGTCATCACCTACATCCAAAGCGAAGCAGG
GCAAAGCGAAAGCGTCGCGCTGGCCCTCAACACCCGCGTCATGGGGTTGCTGCTGATCTTCTATCCCATTGCCGCCTGGA
TCTCAGATCGGCTGGGACGCAAACCGCTACTCATCGCTGGCTCCCTGCTACTCGTGCTCGCAGGCCTACCGATTTTTGAG
CTGCTGCACAGCGGCAATCCCACATGGATCAGCAGGGGCGAAATACTGCTGATGCTGGCGGTGGCCTTGTTGGCCGGAGC
CAAAAACCCAGCCAATGTCGAGTTGATGCCGCAAGCGGTGCGCTGCACAGGTTTGGCCTTGGCGTTCAATGTTGCTGAGG
GATATTTCGGTGGCACCACCCCGCTGATCGCCAGCTGGCTGGTGAGCAACAGTGGTAATCCTCTGCTGCCGGGCTACTGG
GTTGCCTTGGCTGGAGCCATCACCTTGGTCACCGCGCTGTGGTTCACCCCGGAGAGCTGCCGGCTGCCCTTGCAACGTTT
ATGA

Upstream 100 bases:

>100_bases
GCTGATGGGCCGCACGCAGCTGGGCGGCGGCAGCAGCGACGGAGGCGAGATCCGCCAGATCCAAAGGCAGCCACTCCACC
GAACCCCTCGGGGCTTCAGC

Downstream 100 bases:

>100_bases
CAAAGAAGCGGCCAGTGAGCCTTCCATGAAGAAGTGGCTCCCAATCCTGGTGCTGGGGCTCACCCAATTCGTCATGGTGC
TGGATGGCACGGTGATGAAT

Product: MFS superfamily proline/ betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MDALDGLFGPLLVAAAQQHLGTFAGELQSSFITNSRVGACDKGEPTGLISHGISGPTCHRSAANVVVLANPCRVLTSFLI
VSASKQAAMGAHQPPLWRVILAGLIGNVMEWYDFALYGYFVGIFGDQFFPSSDPNASLIAAFGAFAAGFLVRPLGGVVLG
RIGDRVGRRQALTLSVMAMALATVSMAILPTYQQVGLWAPIALVLLRILQGLSAGGEYTTSIVFLAEHAPANRRGLVTIW
GLWGSVLGMLLGSAAGTLLSDSLSAAQMSSWGWRLPFALGLLVALTGFVLRRGLNSETPPAASAQPVRALARHSGAVLRV
LLLNVASSVAFYTAFVYVITYIQSEAGQSESVALALNTRVMGLLLIFYPIAAWISDRLGRKPLLIAGSLLLVLAGLPIFE
LLHSGNPTWISRGEILLMLAVALLAGAKNPANVELMPQAVRCTGLALAFNVAEGYFGGTTPLIASWLVSNSGNPLLPGYW
VALAGAITLVTALWFTPESCRLPLQRL

Sequences:

>Translated_507_residues
MDALDGLFGPLLVAAAQQHLGTFAGELQSSFITNSRVGACDKGEPTGLISHGISGPTCHRSAANVVVLANPCRVLTSFLI
VSASKQAAMGAHQPPLWRVILAGLIGNVMEWYDFALYGYFVGIFGDQFFPSSDPNASLIAAFGAFAAGFLVRPLGGVVLG
RIGDRVGRRQALTLSVMAMALATVSMAILPTYQQVGLWAPIALVLLRILQGLSAGGEYTTSIVFLAEHAPANRRGLVTIW
GLWGSVLGMLLGSAAGTLLSDSLSAAQMSSWGWRLPFALGLLVALTGFVLRRGLNSETPPAASAQPVRALARHSGAVLRV
LLLNVASSVAFYTAFVYVITYIQSEAGQSESVALALNTRVMGLLLIFYPIAAWISDRLGRKPLLIAGSLLLVLAGLPIFE
LLHSGNPTWISRGEILLMLAVALLAGAKNPANVELMPQAVRCTGLALAFNVAEGYFGGTTPLIASWLVSNSGNPLLPGYW
VALAGAITLVTALWFTPESCRLPLQRL
>Mature_507_residues
MDALDGLFGPLLVAAAQQHLGTFAGELQSSFITNSRVGACDKGEPTGLISHGISGPTCHRSAANVVVLANPCRVLTSFLI
VSASKQAAMGAHQPPLWRVILAGLIGNVMEWYDFALYGYFVGIFGDQFFPSSDPNASLIAAFGAFAAGFLVRPLGGVVLG
RIGDRVGRRQALTLSVMAMALATVSMAILPTYQQVGLWAPIALVLLRILQGLSAGGEYTTSIVFLAEHAPANRRGLVTIW
GLWGSVLGMLLGSAAGTLLSDSLSAAQMSSWGWRLPFALGLLVALTGFVLRRGLNSETPPAASAQPVRALARHSGAVLRV
LLLNVASSVAFYTAFVYVITYIQSEAGQSESVALALNTRVMGLLLIFYPIAAWISDRLGRKPLLIAGSLLLVLAGLPIFE
LLHSGNPTWISRGEILLMLAVALLAGAKNPANVELMPQAVRCTGLALAFNVAEGYFGGTTPLIASWLVSNSGNPLLPGYW
VALAGAITLVTALWFTPESCRLPLQRL

Specific function: Involved in uptake and accumulation of various osmoprotectants. Allows the uptake of glycine betaine, proline, ectoine, and pipecolic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=432, Percent_Identity=33.1018518518519, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1788292, Length=389, Percent_Identity=33.4190231362468, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI1788942, Length=389, Percent_Identity=32.3907455012853, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789941, Length=421, Percent_Identity=32.0665083135392, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87082231, Length=218, Percent_Identity=26.1467889908257, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 53416; Mature: 53416

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALDGLFGPLLVAAAQQHLGTFAGELQSSFITNSRVGACDKGEPTGLISHGISGPTCHR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCC
SAANVVVLANPCRVLTSFLIVSASKQAAMGAHQPPLWRVILAGLIGNVMEWYDFALYGYF
CCCCEEEEECHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VGIFGDQFFPSSDPNASLIAAFGAFAAGFLVRPLGGVVLGRIGDRVGRRQALTLSVMAMA
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LATVSMAILPTYQQVGLWAPIALVLLRILQGLSAGGEYTTSIVFLAEHAPANRRGLVTIW
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCEEEH
GLWGSVLGMLLGSAAGTLLSDSLSAAQMSSWGWRLPFALGLLVALTGFVLRRGLNSETPP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
AASAQPVRALARHSGAVLRVLLLNVASSVAFYTAFVYVITYIQSEAGQSESVALALNTRV
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHH
MGLLLIFYPIAAWISDRLGRKPLLIAGSLLLVLAGLPIFELLHSGNPTWISRGEILLMLA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCEEECCHHHHHHH
VALLAGAKNPANVELMPQAVRCTGLALAFNVAEGYFGGTTPLIASWLVSNSGNPLLPGYW
HHHHHCCCCCCCCEECCHHHHHHHHHHEEEHHHCCCCCCHHHHHHHHHCCCCCCCCCHHH
VALAGAITLVTALWFTPESCRLPLQRL
HHHHHHHHHHHHHHCCCHHHCCCHHCC
>Mature Secondary Structure
MDALDGLFGPLLVAAAQQHLGTFAGELQSSFITNSRVGACDKGEPTGLISHGISGPTCHR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCC
SAANVVVLANPCRVLTSFLIVSASKQAAMGAHQPPLWRVILAGLIGNVMEWYDFALYGYF
CCCCEEEEECHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VGIFGDQFFPSSDPNASLIAAFGAFAAGFLVRPLGGVVLGRIGDRVGRRQALTLSVMAMA
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LATVSMAILPTYQQVGLWAPIALVLLRILQGLSAGGEYTTSIVFLAEHAPANRRGLVTIW
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCEEEH
GLWGSVLGMLLGSAAGTLLSDSLSAAQMSSWGWRLPFALGLLVALTGFVLRRGLNSETPP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
AASAQPVRALARHSGAVLRVLLLNVASSVAFYTAFVYVITYIQSEAGQSESVALALNTRV
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHH
MGLLLIFYPIAAWISDRLGRKPLLIAGSLLLVLAGLPIFELLHSGNPTWISRGEILLMLA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCEEECCHHHHHHH
VALLAGAKNPANVELMPQAVRCTGLALAFNVAEGYFGGTTPLIASWLVSNSGNPLLPGYW
HHHHHCCCCCCCCEECCHHHHHHHHHHEEEHHHCCCCCCHHHHHHHHHCCCCCCCCCHHH
VALAGAITLVTALWFTPESCRLPLQRL
HHHHHHHHHHHHHHCCCHHHCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8550465 [H]