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The map label for this gene is gloB [H]

Identifier: 148241938

GI number: 148241938

Start: 763851

End: 764774

Strand: Direct

Name: gloB [H]

Synonym: SynRCC307_0839

Alternate gene names: 148241938

Gene position: 763851-764774 (Clockwise)

Preceding gene: 148241935

Following gene: 148241939

Centisome position: 34.33

GC content: 63.1

Gene sequence:

>924_bases
ATGGCGCTGAAGTCGAGGCCGGCTGCAGCAAAGCGGGCCACCGAATCCTTCAGCAGTGCGCCTTTGGGCAGAGCGACGGT
GAGCATGCTGTTTGCGCTCCAGCGCTGGACTTTAACGAGCAGCCTTCACACTGGTGAGAGAAGCCTTGTCTGCGTGGAGA
TCCAGCGGCTACCCGTGCTGCGGGACAACTATGTGTTTGTGCTGGTGGATCCCGACGCAGCCGCAGCCGCCGTTGTTGAT
CCAGCAGTGGCCGAACCGGTGATTGCGGCCTTGGAGGCCCAACAGCTCCGGCTCAGCGTGGTGCTGCAAACCCACCACCA
CAAAGATCACATTGGCGGCACCCCAGCGCTGCTCAGCCGCTGGCCTGGCGCTCAGGTTTGGGCCGCGGCCTCCGACCGCG
CTCGCATCCCCTTCCAAACCGATGGGCTGCGCGGGGGGGATCAACGGCTGCTGTTCAACCGCCCAGTGCAGGTGTTGGAG
GTTCCTGGCCACACCCGCGATCACATCGCCTTTTTCTTCCCGGCTAGCGGCGGGGAAGGCCCTGAACTGTTTTGCGGCGA
TACCCTGTTTGCAGGCGGTTGCGGGCGGCTGTTTGAAGGCACCGCCGAGCAGATGCTGCGCTCCCTCGATCAGTTGAGCG
GCTTGCCGGAAGCTACCCGGATCTGGTGCGCCCATGAGTACACCGAAGCCAACTTGCGCTTTGCGATCACCCAGGAGCCA
GGCAACCCAGCCCTGCAAGAACGATTTCAGCAGGTGCAAGCCATGCGAGCGGCTGGGGAAGCCACAGTTCCATCGTCGGT
GGCCCTCGAGCGCGCCACCAATCCATTTTTGCGCTGCCGTGAAGCGTCCCTGCAGCAGGTCAGTGGATGCTGTGAACCAG
CCTTGGTGTTGGCCGAAATCCGGCGCCGTAAAGACGTGTTCTGA

Upstream 100 bases:

>100_bases
ATAGGCCACATACACCGGCACATCCCCATTGCGCACCAGCAGGGCCCGGGCGCGGCCGCAGGCGCTGGGCAGCATCAACT
GGCGGTTGTCGGCCGCGGTG

Downstream 100 bases:

>100_bases
GCTGGGAAGTGGGCAGGGATGATGGAGCCAGATGGTTCATCCCGATGAGCGCTGCTCCCCTCGAAGCTGTTGAAACGCAG
GCCGCTCCAGCCCCGGTGGG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MALKSRPAAAKRATESFSSAPLGRATVSMLFALQRWTLTSSLHTGERSLVCVEIQRLPVLRDNYVFVLVDPDAAAAAVVD
PAVAEPVIAALEAQQLRLSVVLQTHHHKDHIGGTPALLSRWPGAQVWAAASDRARIPFQTDGLRGGDQRLLFNRPVQVLE
VPGHTRDHIAFFFPASGGEGPELFCGDTLFAGGCGRLFEGTAEQMLRSLDQLSGLPEATRIWCAHEYTEANLRFAITQEP
GNPALQERFQQVQAMRAAGEATVPSSVALERATNPFLRCREASLQQVSGCCEPALVLAEIRRRKDVF

Sequences:

>Translated_307_residues
MALKSRPAAAKRATESFSSAPLGRATVSMLFALQRWTLTSSLHTGERSLVCVEIQRLPVLRDNYVFVLVDPDAAAAAVVD
PAVAEPVIAALEAQQLRLSVVLQTHHHKDHIGGTPALLSRWPGAQVWAAASDRARIPFQTDGLRGGDQRLLFNRPVQVLE
VPGHTRDHIAFFFPASGGEGPELFCGDTLFAGGCGRLFEGTAEQMLRSLDQLSGLPEATRIWCAHEYTEANLRFAITQEP
GNPALQERFQQVQAMRAAGEATVPSSVALERATNPFLRCREASLQQVSGCCEPALVLAEIRRRKDVF
>Mature_306_residues
ALKSRPAAAKRATESFSSAPLGRATVSMLFALQRWTLTSSLHTGERSLVCVEIQRLPVLRDNYVFVLVDPDAAAAAVVDP
AVAEPVIAALEAQQLRLSVVLQTHHHKDHIGGTPALLSRWPGAQVWAAASDRARIPFQTDGLRGGDQRLLFNRPVQVLEV
PGHTRDHIAFFFPASGGEGPELFCGDTLFAGGCGRLFEGTAEQMLRSLDQLSGLPEATRIWCAHEYTEANLRFAITQEPG
NPALQERFQQVQAMRAAGEATVPSSVALERATNPFLRCREASLQQVSGCCEPALVLAEIRRRKDVF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=258, Percent_Identity=39.1472868217054, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI94538322, Length=258, Percent_Identity=39.1472868217054, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI116642887, Length=265, Percent_Identity=38.4905660377359, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI21703352, Length=265, Percent_Identity=38.4905660377359, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI14150041, Length=257, Percent_Identity=34.6303501945525, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI46361987, Length=204, Percent_Identity=35.2941176470588, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1786406, Length=260, Percent_Identity=36.9230769230769, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17536925, Length=262, Percent_Identity=36.6412213740458, Blast_Score=163, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6320478, Length=245, Percent_Identity=29.3877551020408, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324614, Length=259, Percent_Identity=27.7992277992278, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21356335, Length=259, Percent_Identity=41.6988416988417, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24667711, Length=259, Percent_Identity=41.6988416988417, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24667703, Length=259, Percent_Identity=41.6988416988417, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 33458; Mature: 33326

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKSRPAAAKRATESFSSAPLGRATVSMLFALQRWTLTSSLHTGERSLVCVEIQRLPVL
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEE
RDNYVFVLVDPDAAAAAVVDPAVAEPVIAALEAQQLRLSVVLQTHHHKDHIGGTPALLSR
ECCEEEEEECCCCCHHHHHCHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHC
WPGAQVWAAASDRARIPFQTDGLRGGDQRLLFNRPVQVLEVPGHTRDHIAFFFPASGGEG
CCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCEEEEEECCCCCC
PELFCGDTLFAGGCGRLFEGTAEQMLRSLDQLSGLPEATRIWCAHEYTEANLRFAITQEP
CEEEECCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEEEEECCCCCCEEEEEEECC
GNPALQERFQQVQAMRAAGEATVPSSVALERATNPFLRCREASLQQVSGCCEPALVLAEI
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
RRRKDVF
HHHCCCC
>Mature Secondary Structure 
ALKSRPAAAKRATESFSSAPLGRATVSMLFALQRWTLTSSLHTGERSLVCVEIQRLPVL
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEE
RDNYVFVLVDPDAAAAAVVDPAVAEPVIAALEAQQLRLSVVLQTHHHKDHIGGTPALLSR
ECCEEEEEECCCCCHHHHHCHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHC
WPGAQVWAAASDRARIPFQTDGLRGGDQRLLFNRPVQVLEVPGHTRDHIAFFFPASGGEG
CCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCEEEEEECCCCCC
PELFCGDTLFAGGCGRLFEGTAEQMLRSLDQLSGLPEATRIWCAHEYTEANLRFAITQEP
CEEEECCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEEEEECCCCCCEEEEEEECC
GNPALQERFQQVQAMRAAGEATVPSSVALERATNPFLRCREASLQQVSGCCEPALVLAEI
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
RRRKDVF
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA