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The map label for this gene is thiC [H]

Identifier: 148241228

GI number: 148241228

Start: 123610

End: 125010

Strand: Direct

Name: thiC [H]

Synonym: SynRCC307_0129

Alternate gene names: 148241228

Gene position: 123610-125010 (Clockwise)

Preceding gene: 148241227

Following gene: 148241232

Centisome position: 5.56

GC content: 59.89

Gene sequence:

>1401_bases
ATGCGCAGTGCCTGGATTGAGAAGCGCCGCGGCAGTGCCAATGTCAGCCAGCTCCATTACGCCCGCCAGGGCGTGGTTAC
CGAAGAAATGGCCTATGTGGCCAAACGGGAGAACCTGCCGGAATCGCTGGTGATGGAGGAGGTGGCTCGGGGTCGCATGA
TCATCCCGGCCAACATCAACCACGCGAATCTGGAGCCGATGGCCATCGGCATCGCCAGCAAGTGCAAGGTGAACGCGAAT
ATCGGCGCGTCCCCCAATGCCTCCGATGCCTCGGAAGAGGTGAAGAAGCTGGAGCTGGCTGTGAAATATGGCGCCGACAC
CGTGATGGATCTCTCAACCGGTGGCGTGAACCTCGATGAGGTGCGCACGGCGATCATCAACGCGTCGCCAGTGCCTATCG
GCACCGTGCCCGTCTACCAGGCCCTCGAGAGCGTGCATGGCTCGATCGAGCGCCTCTCAGAAGATGATTTCCTGCACATC
ATCGAGAAGCACTGCCAGCAAGGCGTTGATTACCAAACGATCCATGCGGGCCTGCTGATTGAGCACCTGCCCAAAGTCAA
GGGCCGTTTGACCGGGATCGTCAGCCGTGGTGGCGGGATCTTGGCCCAGTGGATGCTCTATCACCACAAGCAGAACCCTC
TCTACACCCGCTTTGAAGACATCTGCGAGATCTTCAAGCGCTACGACTGCACCTTCTCCTTAGGTGACTCTTTGCGGCCT
GGCTGTCAGCACGATGCCTCTGACGATGCCCAGCTGGCTGAACTCAAGACCCTGGGAGAGCTCACCCGCCGCGCTTGGAA
GCATGACGTTCAGGTGATGGTGGAAGGCCCCGGCCATGTGCCGATGGACCAGATCGAGTTCAACGTCAAAAAGCAGATGG
AGGAGTGCAGCGAAGCCCCCTTCTACGTGCTCGGTCCACTCGTGACTGACATCGCTCCTGGCTACGACCACATCACCAGT
GCCATTGGTGCTGCGATGGCCGGCTGGCATGGCACCGCCATGCTCTGCTATGTCACCCCCAAAGAACACCTGGGTCTGCC
GAACGCAGACGATGTGCGGGAGGGCTTAATCGCCTACAAGATCGCTGCCCACGCCGCCGACATTGCCCGACACCGTCCTG
GTGCCCGTGACCGCGACGACGAGCTCAGCCGGGCGCGCTACGCCTTCGACTGGAACAAGCAGTTTGAGTTGTCCCTCGAT
CCTGAGCGGGCCAAGGAGTATCACGACGAAACCCTGCCAGCCGATATCTATAAGCAGGCAGAGTTTTGCTCGATGTGTGG
TCCAAAGCACTGCCCGATGCAAACCAAGATCACTGATAAAGACCTCGAGAGCCTGGAAGAGGTGCTCAAGGCCAAGGGCG
GGGCTGAGCTGAGTACGGCCAAGCTCGATCGGGCGGACTAA

Upstream 100 bases:

>100_bases
TATGGCTGAGATCACACCCTCCGAACCTGATTCCGGGTTATGCCGGCGCAGGGAAGTGCTGGATGTGCGGCGGCCCCCTC
CCTGTTGAGGAACCTTCGTC

Downstream 100 bases:

>100_bases
TCCTCTTTCGCTCAGCTCACAGGCACAAAAAAAGCCCCCCTTTTGGGGGGCTTTTGTTTGGCTAAAGGAGGCTCAACCCA
GCAGGGCTTTGGACTTGGCC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MRSAWIEKRRGSANVSQLHYARQGVVTEEMAYVAKRENLPESLVMEEVARGRMIIPANINHANLEPMAIGIASKCKVNAN
IGASPNASDASEEVKKLELAVKYGADTVMDLSTGGVNLDEVRTAIINASPVPIGTVPVYQALESVHGSIERLSEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPKVKGRLTGIVSRGGGILAQWMLYHHKQNPLYTRFEDICEIFKRYDCTFSLGDSLRP
GCQHDASDDAQLAELKTLGELTRRAWKHDVQVMVEGPGHVPMDQIEFNVKKQMEECSEAPFYVLGPLVTDIAPGYDHITS
AIGAAMAGWHGTAMLCYVTPKEHLGLPNADDVREGLIAYKIAAHAADIARHRPGARDRDDELSRARYAFDWNKQFELSLD
PERAKEYHDETLPADIYKQAEFCSMCGPKHCPMQTKITDKDLESLEEVLKAKGGAELSTAKLDRAD

Sequences:

>Translated_466_residues
MRSAWIEKRRGSANVSQLHYARQGVVTEEMAYVAKRENLPESLVMEEVARGRMIIPANINHANLEPMAIGIASKCKVNAN
IGASPNASDASEEVKKLELAVKYGADTVMDLSTGGVNLDEVRTAIINASPVPIGTVPVYQALESVHGSIERLSEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPKVKGRLTGIVSRGGGILAQWMLYHHKQNPLYTRFEDICEIFKRYDCTFSLGDSLRP
GCQHDASDDAQLAELKTLGELTRRAWKHDVQVMVEGPGHVPMDQIEFNVKKQMEECSEAPFYVLGPLVTDIAPGYDHITS
AIGAAMAGWHGTAMLCYVTPKEHLGLPNADDVREGLIAYKIAAHAADIARHRPGARDRDDELSRARYAFDWNKQFELSLD
PERAKEYHDETLPADIYKQAEFCSMCGPKHCPMQTKITDKDLESLEEVLKAKGGAELSTAKLDRAD
>Mature_466_residues
MRSAWIEKRRGSANVSQLHYARQGVVTEEMAYVAKRENLPESLVMEEVARGRMIIPANINHANLEPMAIGIASKCKVNAN
IGASPNASDASEEVKKLELAVKYGADTVMDLSTGGVNLDEVRTAIINASPVPIGTVPVYQALESVHGSIERLSEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPKVKGRLTGIVSRGGGILAQWMLYHHKQNPLYTRFEDICEIFKRYDCTFSLGDSLRP
GCQHDASDDAQLAELKTLGELTRRAWKHDVQVMVEGPGHVPMDQIEFNVKKQMEECSEAPFYVLGPLVTDIAPGYDHITS
AIGAAMAGWHGTAMLCYVTPKEHLGLPNADDVREGLIAYKIAAHAADIARHRPGARDRDDELSRARYAFDWNKQFELSLD
PERAKEYHDETLPADIYKQAEFCSMCGPKHCPMQTKITDKDLESLEEVLKAKGGAELSTAKLDRAD

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=485, Percent_Identity=54.4329896907216, Blast_Score=526, Evalue=1e-151,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 51652; Mature: 51652

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSAWIEKRRGSANVSQLHYARQGVVTEEMAYVAKRENLPESLVMEEVARGRMIIPANIN
CCCCHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HANLEPMAIGIASKCKVNANIGASPNASDASEEVKKLELAVKYGADTVMDLSTGGVNLDE
CCCCCCEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
VRTAIINASPVPIGTVPVYQALESVHGSIERLSEDDFLHIIEKHCQQGVDYQTIHAGLLI
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHH
EHLPKVKGRLTGIVSRGGGILAQWMLYHHKQNPLYTRFEDICEIFKRYDCTFSLGDSLRP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEECCCCCCC
GCQHDASDDAQLAELKTLGELTRRAWKHDVQVMVEGPGHVPMDQIEFNVKKQMEECSEAP
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDIAPGYDHITSAIGAAMAGWHGTAMLCYVTPKEHLGLPNADDVREGLIAYK
EEEEHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHH
IAAHAADIARHRPGARDRDDELSRARYAFDWNKQFELSLDPERAKEYHDETLPADIYKQA
HHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCEEEEEECHHHHHHHHCCCCCHHHHHHH
EFCSMCGPKHCPMQTKITDKDLESLEEVLKAKGGAELSTAKLDRAD
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRSAWIEKRRGSANVSQLHYARQGVVTEEMAYVAKRENLPESLVMEEVARGRMIIPANIN
CCCCHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HANLEPMAIGIASKCKVNANIGASPNASDASEEVKKLELAVKYGADTVMDLSTGGVNLDE
CCCCCCEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
VRTAIINASPVPIGTVPVYQALESVHGSIERLSEDDFLHIIEKHCQQGVDYQTIHAGLLI
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHH
EHLPKVKGRLTGIVSRGGGILAQWMLYHHKQNPLYTRFEDICEIFKRYDCTFSLGDSLRP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEECCCCCCC
GCQHDASDDAQLAELKTLGELTRRAWKHDVQVMVEGPGHVPMDQIEFNVKKQMEECSEAP
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDIAPGYDHITSAIGAAMAGWHGTAMLCYVTPKEHLGLPNADDVREGLIAYK
EEEEHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHH
IAAHAADIARHRPGARDRDDELSRARYAFDWNKQFELSLDPERAKEYHDETLPADIYKQA
HHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCEEEEEECHHHHHHHHCCCCCHHHHHHH
EFCSMCGPKHCPMQTKITDKDLESLEEVLKAKGGAELSTAKLDRAD
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA