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The map label for this gene is proB

Identifier: 162329621

GI number: 162329621

Start: 1981604

End: 1982737

Strand: Reverse

Name: proB

Synonym: VC0395_A1864

Alternate gene names: 162329621

Gene position: 1982737-1981604 (Counterclockwise)

Preceding gene: 147673803

Following gene: 147674854

Centisome position: 65.57

GC content: 52.29

Gene sequence:

>1134_bases
ATGACCACAAATCAACGAAGCATCAAAGCTCCACAAACGGTTGTTGTGAAGCTGGGAACCAGTGTTCTGACCGGCGGTAC
TCTTGCGCTGGATCGTGCTCATATGGTTGAGCTCGCTCGCCAGTGTGCTGAACTCAAAAAGCAAGGTCACTCAGTAGTGA
TCGTGACCTCGGGCGCCATTGCGGCTGGCCGTGAGCATTTAGGTTACCCTGCGCTGCCCAACGCGATTTCCAGCAAGCAA
CTGCTGGCAGCGGTTGGGCAAAGTCGTTTGATTCAGGTCTGGGAATCGTTATTTGCGATTTATGGCATCAAAATTGGTCA
GATGCTGCTCACCCGTGCTGATCTTGATGACCGTGAGCGTTTCCTTAATGCGCGTGACACCATCAATGCGTTAGTTGAAA
ATGGCATTGTGCCTGTGGTCAACGAAAACGATGCGGTAGCAACCAATGAAATCAAAGTCGGTGACAACGATAACTTATCG
GCGCTGGTCGGTATTTTGTGTGGTGCGGATAAGCTGCTGCTGTTGACCGATCAAAAAGGCTTGTTCACCGCCGATCCGCG
TAAAGATCCCAATGCCCAACTGATCAAAGAAGTGACCACCATTGATGACACACTGCGCAAAATTGCTGGTGGCAGTGGCA
CTACACTCGGTACGGGCGGCATGGCCACCAAGCTGCAAGCGGCGGACATTGCTCGTCGTGCTGGTATTGAAGTCATCATT
GCGGCAGGTCGTGCGCCCAATGTGATTTTTGACTCCTTAAGTGATGAGCCACAAGGCACGCGCTTCTTAGCTTTGGAAGA
AGCGTTAGAAAACCGCAAACGCTGGATTTTGGCAGGCCCAGCGGCATCAGGCGATATTGTGATTGACGATGGCGCAGTGA
AAGCGGTGCAAACCAAGGGCAGCAGCTTGCTCGCCAAAGGTGTGGTGCGAGTGCAAGGTCAGTTCGCCCGTGGTGAAGTG
GTGCGGGTACTGGATAAGCAAAGTCATCTGATTGCGCGCGGCATCGCGGCCTACTCCAATGACGAGTTGGCGCAAATTGC
TGGTAAGCACAGTAAAGAGATCATCGATATTTTAGGCTACGATCACGGCTCAGAAGTGATCCACCGCGACGACATGGTCG
TGATTCAAGAGTAA

Upstream 100 bases:

>100_bases
ACCCGAAGAAAAAGGTGCTTTTGCACCTTTTTCTGCTTGTGCATTGCAGTGTGGATTGCTAAAACATCGTCTCTTAAAGT
TTTTCTCAACAGAAGCAATC

Downstream 100 bases:

>100_bases
AGGAGAGAATGATGGATTTAACCGTACTCGGTAAAGCGGCCAAAGCGGCCTCTTTCCAACTGGCTACCGCCAGTACCGCG
CAAAAAAATCAGGCTCTCGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MTTNQRSIKAPQTVVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVIVTSGAIAAGREHLGYPALPNAISSKQ
LLAAVGQSRLIQVWESLFAIYGIKIGQMLLTRADLDDRERFLNARDTINALVENGIVPVVNENDAVATNEIKVGDNDNLS
ALVGILCGADKLLLLTDQKGLFTADPRKDPNAQLIKEVTTIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEVII
AAGRAPNVIFDSLSDEPQGTRFLALEEALENRKRWILAGPAASGDIVIDDGAVKAVQTKGSSLLAKGVVRVQGQFARGEV
VRVLDKQSHLIARGIAAYSNDELAQIAGKHSKEIIDILGYDHGSEVIHRDDMVVIQE

Sequences:

>Translated_377_residues
MTTNQRSIKAPQTVVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVIVTSGAIAAGREHLGYPALPNAISSKQ
LLAAVGQSRLIQVWESLFAIYGIKIGQMLLTRADLDDRERFLNARDTINALVENGIVPVVNENDAVATNEIKVGDNDNLS
ALVGILCGADKLLLLTDQKGLFTADPRKDPNAQLIKEVTTIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEVII
AAGRAPNVIFDSLSDEPQGTRFLALEEALENRKRWILAGPAASGDIVIDDGAVKAVQTKGSSLLAKGVVRVQGQFARGEV
VRVLDKQSHLIARGIAAYSNDELAQIAGKHSKEIIDILGYDHGSEVIHRDDMVVIQE
>Mature_376_residues
TTNQRSIKAPQTVVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVIVTSGAIAAGREHLGYPALPNAISSKQL
LAAVGQSRLIQVWESLFAIYGIKIGQMLLTRADLDDRERFLNARDTINALVENGIVPVVNENDAVATNEIKVGDNDNLSA
LVGILCGADKLLLLTDQKGLFTADPRKDPNAQLIKEVTTIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEVIIA
AGRAPNVIFDSLSDEPQGTRFLALEEALENRKRWILAGPAASGDIVIDDGAVKAVQTKGSSLLAKGVVRVQGQFARGEVV
RVLDKQSHLIARGIAAYSNDELAQIAGKHSKEIIDILGYDHGSEVIHRDDMVVIQE

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=302, Percent_Identity=32.7814569536424, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI62912457, Length=300, Percent_Identity=32.6666666666667, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1786437, Length=362, Percent_Identity=66.5745856353591, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17569969, Length=280, Percent_Identity=32.5, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17569967, Length=280, Percent_Identity=32.5, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321822, Length=412, Percent_Identity=36.6504854368932, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6320506, Length=413, Percent_Identity=36.5617433414044, Blast_Score=228, Evalue=1e-60,
Organism=Drosophila melanogaster, GI21357643, Length=300, Percent_Identity=33, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_VIBCH (Q9KPT8)

Other databases:

- EMBL:   AE003852
- PIR:   F82095
- RefSeq:   NP_231905.2
- ProteinModelPortal:   Q9KPT8
- SMR:   Q9KPT8
- GeneID:   2613196
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2274
- TIGR:   VC_2274
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40152; Mature: 40020

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNQRSIKAPQTVVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVIVTSGAI
CCCCCCCCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCH
AAGREHLGYPALPNAISSKQLLAAVGQSRLIQVWESLFAIYGIKIGQMLLTRADLDDRER
HCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FLNARDTINALVENGIVPVVNENDAVATNEIKVGDNDNLSALVGILCGADKLLLLTDQKG
HHHHHHHHHHHHHCCCEEEECCCCCEEECEEEECCCCCHHHHHHHHHCCCEEEEEECCCC
LFTADPRKDPNAQLIKEVTTIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEVII
CEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCCEEEE
AAGRAPNVIFDSLSDEPQGTRFLALEEALENRKRWILAGPAASGDIVIDDGAVKAVQTKG
ECCCCCCHHHHCCCCCCCCCEEEHHHHHHHCCCEEEEECCCCCCCEEEECCCEEEECCCC
SSLLAKGVVRVQGQFARGEVVRVLDKQSHLIARGIAAYSNDELAQIAGKHSKEIIDILGY
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCC
DHGSEVIHRDDMVVIQE
CCCCCEEECCCEEEEEC
>Mature Secondary Structure 
TTNQRSIKAPQTVVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVIVTSGAI
CCCCCCCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCH
AAGREHLGYPALPNAISSKQLLAAVGQSRLIQVWESLFAIYGIKIGQMLLTRADLDDRER
HCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FLNARDTINALVENGIVPVVNENDAVATNEIKVGDNDNLSALVGILCGADKLLLLTDQKG
HHHHHHHHHHHHHCCCEEEECCCCCEEECEEEECCCCCHHHHHHHHHCCCEEEEEECCCC
LFTADPRKDPNAQLIKEVTTIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEVII
CEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCCEEEE
AAGRAPNVIFDSLSDEPQGTRFLALEEALENRKRWILAGPAASGDIVIDDGAVKAVQTKG
ECCCCCCHHHHCCCCCCCCCEEEHHHHHHHCCCEEEEECCCCCCCEEEECCCEEEECCCC
SSLLAKGVVRVQGQFARGEVVRVLDKQSHLIARGIAAYSNDELAQIAGKHSKEIIDILGY
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCC
DHGSEVIHRDDMVVIQE
CCCCCEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301