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The map label for this gene is rpfG [H]

Identifier: 147675731

GI number: 147675731

Start: 2235096

End: 2236349

Strand: Reverse

Name: rpfG [H]

Synonym: VC0395_A2078

Alternate gene names: 147675731

Gene position: 2236349-2235096 (Counterclockwise)

Preceding gene: 147674275

Following gene: 147674909

Centisome position: 73.95

GC content: 44.1

Gene sequence:

>1254_bases
GTGGCAAGCATTAAAATCACGGTAGATAGATTGCAACCCGGGCTCTACATTCGGTTGCCTGTCAAATGGAATGAACATCC
CTTCCTATTCAATAGCTTTAAGATCAAATCACAAGAGCAGATCCAACTGATCAAGCATCTTGGTATTCAGCACGTTTTTC
TCAATCCGGGATTAAGCGATAGCCAGCCTTTACCGCCAAGCGTTGAAGAGGCAACTCCAGAAGAGGCTCTACCTCCGGTT
GATAGTGAAGCGGAAAAGCTATGGCAGGAAAAGCAAACACGAATAGAGAAGCTCAACGAATATCGACGTCGAGTACAAAC
TTGTGAGAAAGAGTTTGAACGTTCTCTTGCTCGTATGCGTGCGGTAGTGAATAAAATTCGTAACCGCCCTTTAGATGCCG
TCGGCGAAGCCATCATATTGGTCGATGATATCGTCGACAAATTGCTGAGTGATGATAATGTCACCTTGCATTTAATGAAC
AGCAAAAGTGAATTTGAAGATATCTATTTTCACTCTTTAAACGTGTCAGTCATTGCCATGATGATTGGTAAAGCGAGAGG
CTTGCCTGCAGAAAGAATCAAAGAGTTGGCTTTTGCGGCGCTATTTCATGACATAGGCAAAGTGAGAGTGCCAACGGCGA
TCGTACGTAAAAAGACCCCATTAACGGAACCGGAAGAAAACTACTTACGGCTGCATACCAAATACGGCATAGAGTTGGCC
GATAATATAGAAACGTTTCCTGAAAGTGCTAAACGCGTCATCGAACAACATCACGAACTGCGAGATGGTTCAGGCTATCC
ACTCGGCCTAAAAGGGGATGATATTGATGAGCTTGCGCAGATTGTGGCGGTAGCGAACGCGTTCGACAATCTTTGCCACC
CCAATATTCCTTCGGAACAAAAGATCCCGTACGTTGCTTTGTCTCATCTATTTAAGAATTGTAAGCATCTTTATAACGCG
GAGAATTTAGGCATCTTAGTTAAGTTCATGGGGGTGTTCCCACCAGGAACTGTCGTACAGCTCTCTAATGATATGGTGGG
GTTAGTCATTTCAGTGAATGCAAGTAGCCTCTTGTATCCGAATGTACTGATTTATGATCCCTCAGTCCCGCGTTCTCAAG
CGACGATAATTGATCTTGCAGACCGAGATCTGAAAATCGTCAATGCCATTTTACCGAACAAATTACCTGATAAGGTTCGT
GATTATCTTAACCCGCGTTCACGGATCTCATACTTCTTTGACAGCGAAGAGTAA

Upstream 100 bases:

>100_bases
GCTTTCGTGATCTAACAACCTTCATAGCAATCAGACTGATGCAGTTATTATTAACTTCTTCTATCATATTCGGGTTATTC
ACTCAGATGTAATCAAGATT

Downstream 100 bases:

>100_bases
AAGAGATCCTTTTAGAGCATTTTGAGATTGAATGGCGCTTAAATCGCGTCATTTTTATGAGAGAAAGAGTGATAAATAAA
CGTCTTGTCGGTTTTGTGTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MASIKITVDRLQPGLYIRLPVKWNEHPFLFNSFKIKSQEQIQLIKHLGIQHVFLNPGLSDSQPLPPSVEEATPEEALPPV
DSEAEKLWQEKQTRIEKLNEYRRRVQTCEKEFERSLARMRAVVNKIRNRPLDAVGEAIILVDDIVDKLLSDDNVTLHLMN
SKSEFEDIYFHSLNVSVIAMMIGKARGLPAERIKELAFAALFHDIGKVRVPTAIVRKKTPLTEPEENYLRLHTKYGIELA
DNIETFPESAKRVIEQHHELRDGSGYPLGLKGDDIDELAQIVAVANAFDNLCHPNIPSEQKIPYVALSHLFKNCKHLYNA
ENLGILVKFMGVFPPGTVVQLSNDMVGLVISVNASSLLYPNVLIYDPSVPRSQATIIDLADRDLKIVNAILPNKLPDKVR
DYLNPRSRISYFFDSEE

Sequences:

>Translated_417_residues
MASIKITVDRLQPGLYIRLPVKWNEHPFLFNSFKIKSQEQIQLIKHLGIQHVFLNPGLSDSQPLPPSVEEATPEEALPPV
DSEAEKLWQEKQTRIEKLNEYRRRVQTCEKEFERSLARMRAVVNKIRNRPLDAVGEAIILVDDIVDKLLSDDNVTLHLMN
SKSEFEDIYFHSLNVSVIAMMIGKARGLPAERIKELAFAALFHDIGKVRVPTAIVRKKTPLTEPEENYLRLHTKYGIELA
DNIETFPESAKRVIEQHHELRDGSGYPLGLKGDDIDELAQIVAVANAFDNLCHPNIPSEQKIPYVALSHLFKNCKHLYNA
ENLGILVKFMGVFPPGTVVQLSNDMVGLVISVNASSLLYPNVLIYDPSVPRSQATIIDLADRDLKIVNAILPNKLPDKVR
DYLNPRSRISYFFDSEE
>Mature_416_residues
ASIKITVDRLQPGLYIRLPVKWNEHPFLFNSFKIKSQEQIQLIKHLGIQHVFLNPGLSDSQPLPPSVEEATPEEALPPVD
SEAEKLWQEKQTRIEKLNEYRRRVQTCEKEFERSLARMRAVVNKIRNRPLDAVGEAIILVDDIVDKLLSDDNVTLHLMNS
KSEFEDIYFHSLNVSVIAMMIGKARGLPAERIKELAFAALFHDIGKVRVPTAIVRKKTPLTEPEENYLRLHTKYGIELAD
NIETFPESAKRVIEQHHELRDGSGYPLGLKGDDIDELAQIVAVANAFDNLCHPNIPSEQKIPYVALSHLFKNCKHLYNAE
NLGILVKFMGVFPPGTVVQLSNDMVGLVISVNASSLLYPNVLIYDPSVPRSQATIIDLADRDLKIVNAILPNKLPDKVRD
YLNPRSRISYFFDSEE

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 47311; Mature: 47180

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIKITVDRLQPGLYIRLPVKWNEHPFLFNSFKIKSQEQIQLIKHLGIQHVFLNPGLSD
CCEEEEEHHHCCCCEEEEEEEEECCCCCEEECEECCCHHHHHHHHHCCCEEEEECCCCCC
SQPLPPSVEEATPEEALPPVDSEAEKLWQEKQTRIEKLNEYRRRVQTCEKEFERSLARMR
CCCCCCCHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVVNKIRNRPLDAVGEAIILVDDIVDKLLSDDNVTLHLMNSKSEFEDIYFHSLNVSVIAM
HHHHHHHCCCHHHHCCEEEHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHH
MIGKARGLPAERIKELAFAALFHDIGKVRVPTAIVRKKTPLTEPEENYLRLHTKYGIELA
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCEEEEECCCCEEH
DNIETFPESAKRVIEQHHELRDGSGYPLGLKGDDIDELAQIVAVANAFDNLCHPNIPSEQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
KIPYVALSHLFKNCKHLYNAENLGILVKFMGVFPPGTVVQLSNDMVGLVISVNASSLLYP
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCEEEEEEEECCCCEECC
NVLIYDPSVPRSQATIIDLADRDLKIVNAILPNKLPDKVRDYLNPRSRISYFFDSEE
EEEEECCCCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHCCHHHHEEEEECCCC
>Mature Secondary Structure 
ASIKITVDRLQPGLYIRLPVKWNEHPFLFNSFKIKSQEQIQLIKHLGIQHVFLNPGLSD
CEEEEEHHHCCCCEEEEEEEEECCCCCEEECEECCCHHHHHHHHHCCCEEEEECCCCCC
SQPLPPSVEEATPEEALPPVDSEAEKLWQEKQTRIEKLNEYRRRVQTCEKEFERSLARMR
CCCCCCCHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVVNKIRNRPLDAVGEAIILVDDIVDKLLSDDNVTLHLMNSKSEFEDIYFHSLNVSVIAM
HHHHHHHCCCHHHHCCEEEHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHH
MIGKARGLPAERIKELAFAALFHDIGKVRVPTAIVRKKTPLTEPEENYLRLHTKYGIELA
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCEEEEECCCCEEH
DNIETFPESAKRVIEQHHELRDGSGYPLGLKGDDIDELAQIVAVANAFDNLCHPNIPSEQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
KIPYVALSHLFKNCKHLYNAENLGILVKFMGVFPPGTVVQLSNDMVGLVISVNASSLLYP
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCEEEEEEEECCCCEECC
NVLIYDPSVPRSQATIIDLADRDLKIVNAILPNKLPDKVRDYLNPRSRISYFFDSEE
EEEEECCCCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHCCHHHHEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]