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The map label for this gene is tcpI

Identifier: 147675729

GI number: 147675729

Start: 377282

End: 379144

Strand: Reverse

Name: tcpI

Synonym: VC0395_A0350

Alternate gene names: 147675729

Gene position: 379144-377282 (Counterclockwise)

Preceding gene: 147674631

Following gene: 147675066

Centisome position: 12.54

GC content: 37.9

Gene sequence:

>1863_bases
ATGATAAAAAAAATAATTTCGGTTTTTTTATTACTTGCGTGCATCATTACGTCGGCTTTTACTGCCTTTTTCTATCACTC
GAAACTATCAGATCAAACAAAATCTATATCTAGCCTTAGTTCTCAGCAGGCACAAGAACGGTTGCAAAGTTATCAGGATT
CTTTAGATTTTTATAAAAAACTTAATATCTCTCTCTCAGTTGCAATAGCCAATTCACTTCGCGATAAAGCAGTCGAAGAA
CTCAACGCTATTGCTTTACGTATACAGGAAAATCATGGATTTATTGGTGTTACCTTCGCTAGTTTAGATGGCACCATGTT
TACAGATATTGGTACTCTAGATTGGAATGCAAAGACATTGAGAAGAGATTGGTTTGTTAAAACGGTCGAACTCGGTACTA
AACATTACACAGCTTTTGATATCGATAAAACAACTGGGCAACACGTTCTCACTATTGCCACCCCTGTTTATGTAGGCAAC
GACATTGTCGGCAGTGTCGCTTTAGATATTGCAGGTGATCAGATTGCCTCACCTAACGGCAGTGGGATGTTTATGATGAC
GGATAGGAATTTTAATGTATTTGCATCTGATCTCACTCACAGTACCTTAATTGGAAAAGATTTAACGAAAGAGAAGCCTC
TGTTCAAAAATCTGGTCTCAGGCCAGTATGTCACCTTCAGTGATGCTGATAGTCATTGGTTTGCAGTGTCACAAACAGAA
ATAGATGGTGAAAATAAACTGTTCACTATTATTGATATTCAACAAATCGTCCAAACATACAAGCGAGATATCCAGTTGAT
TATTGCTGGATTTAGTGGTTTTTCATGCGTGATGCTAATTGGACTATATTGGGTTCTAAGCAAAGAACTCTCAGGTGTTA
GACAGATCAGAGAATGGATTCTTTCCCTTTCTGATGGTCAGATAAAAGAACGCAGGCCTATAAAATTCCATAACGAGCTC
GACACTATTGCCCAAAGTCTTGAAAATTTACAATTTAGGCTTCTTGATGTGGTGAGAAATTCTCATCGGACTATGAATGA
TTTAAGTATCAAACAGACTGACATTACGTATTCAATTGAAGGGAATACGAATAATTCACAGCAAGAACTTGGGCTCATTG
AACAAGTGGCCACAGCAACAACGCAACTATCATGTACTTCTTTTGATGTAATGCAACAAGCTCAAAGTGCAGAGCTTAAT
GCGGAGACGGCCCAAAAATTGATTGCTGAAAGTCATGATATTATCGATTCATCAAGCAAACAAACCGAAATGGTAACCCT
ATCGATCCATGAATCCCAACAAATAATCAATCAGTTACGCGAGTTTTCCGATAATATTAGTTCGGTTACGGATGTCATTA
ATAATATATCAGATCAAACTAACTTACTTGCCCTAAATGCAGCTATCGAGGCTGCTCGTGCAGGTGAACAGGGGCGCGGA
TTTGCTGTAGTAGCGGATGAAGTTCGTTCTCTTGCAGTGAAAACTCAGCAATCGACTATAGATATTCAAGGTATTATTTT
GAAACTGCAAGAGCAATCGCAGTTGGCAGATCAAGTGATGACACGTAATGTTTCACTTATTCATGAAACACAAGTTGCCA
ATCGGGCACTTATCGCATCCTTTAACCTGATTTCTGATAAGGTTCTTGAAATATCAAACATAAACTCCATCGTTTCGACT
GCCGCAAATGAACAAAAAATAGTAACTGAAGATGTAGCAAAACAGATGGAAGATATTCGATATCTAGTTCAAGAGAATTT
ATCCGCGATGGAACGAACTAAACAGGCAAATCAGAATATCTCTGATTTGACCACCAATCTAAATGATGCTCTTAGCTTTT
TTAAAATTGAGCTTACTTCATAA

Upstream 100 bases:

>100_bases
AAAACGATAAAAGCTGATTTTTCACATCATTATTTTATAATTTTATTTTTAATTATCACGAAGTACTCCGTGATAACCAT
TTAAACTTAGAGTTAAACTA

Downstream 100 bases:

>100_bases
ATAGGAAATAATTAGGCTAGTGCCAGTCAGTTGAGCTGACTGGCTTTTTTTCTCGCACAATCGTGTAGCCATGCAACAAC
AACAACAACCTTTACACATG

Product: toxin co-regulated pilus biosynthesis protein I

Products: NA

Alternate protein names: TCP pilus biosynthesis protein tcpI

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MIKKIISVFLLLACIITSAFTAFFYHSKLSDQTKSISSLSSQQAQERLQSYQDSLDFYKKLNISLSVAIANSLRDKAVEE
LNAIALRIQENHGFIGVTFASLDGTMFTDIGTLDWNAKTLRRDWFVKTVELGTKHYTAFDIDKTTGQHVLTIATPVYVGN
DIVGSVALDIAGDQIASPNGSGMFMMTDRNFNVFASDLTHSTLIGKDLTKEKPLFKNLVSGQYVTFSDADSHWFAVSQTE
IDGENKLFTIIDIQQIVQTYKRDIQLIIAGFSGFSCVMLIGLYWVLSKELSGVRQIREWILSLSDGQIKERRPIKFHNEL
DTIAQSLENLQFRLLDVVRNSHRTMNDLSIKQTDITYSIEGNTNNSQQELGLIEQVATATTQLSCTSFDVMQQAQSAELN
AETAQKLIAESHDIIDSSSKQTEMVTLSIHESQQIINQLREFSDNISSVTDVINNISDQTNLLALNAAIEAARAGEQGRG
FAVVADEVRSLAVKTQQSTIDIQGIILKLQEQSQLADQVMTRNVSLIHETQVANRALIASFNLISDKVLEISNINSIVST
AANEQKIVTEDVAKQMEDIRYLVQENLSAMERTKQANQNISDLTTNLNDALSFFKIELTS

Sequences:

>Translated_620_residues
MIKKIISVFLLLACIITSAFTAFFYHSKLSDQTKSISSLSSQQAQERLQSYQDSLDFYKKLNISLSVAIANSLRDKAVEE
LNAIALRIQENHGFIGVTFASLDGTMFTDIGTLDWNAKTLRRDWFVKTVELGTKHYTAFDIDKTTGQHVLTIATPVYVGN
DIVGSVALDIAGDQIASPNGSGMFMMTDRNFNVFASDLTHSTLIGKDLTKEKPLFKNLVSGQYVTFSDADSHWFAVSQTE
IDGENKLFTIIDIQQIVQTYKRDIQLIIAGFSGFSCVMLIGLYWVLSKELSGVRQIREWILSLSDGQIKERRPIKFHNEL
DTIAQSLENLQFRLLDVVRNSHRTMNDLSIKQTDITYSIEGNTNNSQQELGLIEQVATATTQLSCTSFDVMQQAQSAELN
AETAQKLIAESHDIIDSSSKQTEMVTLSIHESQQIINQLREFSDNISSVTDVINNISDQTNLLALNAAIEAARAGEQGRG
FAVVADEVRSLAVKTQQSTIDIQGIILKLQEQSQLADQVMTRNVSLIHETQVANRALIASFNLISDKVLEISNINSIVST
AANEQKIVTEDVAKQMEDIRYLVQENLSAMERTKQANQNISDLTTNLNDALSFFKIELTS
>Mature_620_residues
MIKKIISVFLLLACIITSAFTAFFYHSKLSDQTKSISSLSSQQAQERLQSYQDSLDFYKKLNISLSVAIANSLRDKAVEE
LNAIALRIQENHGFIGVTFASLDGTMFTDIGTLDWNAKTLRRDWFVKTVELGTKHYTAFDIDKTTGQHVLTIATPVYVGN
DIVGSVALDIAGDQIASPNGSGMFMMTDRNFNVFASDLTHSTLIGKDLTKEKPLFKNLVSGQYVTFSDADSHWFAVSQTE
IDGENKLFTIIDIQQIVQTYKRDIQLIIAGFSGFSCVMLIGLYWVLSKELSGVRQIREWILSLSDGQIKERRPIKFHNEL
DTIAQSLENLQFRLLDVVRNSHRTMNDLSIKQTDITYSIEGNTNNSQQELGLIEQVATATTQLSCTSFDVMQQAQSAELN
AETAQKLIAESHDIIDSSSKQTEMVTLSIHESQQIINQLREFSDNISSVTDVINNISDQTNLLALNAAIEAARAGEQGRG
FAVVADEVRSLAVKTQQSTIDIQGIILKLQEQSQLADQVMTRNVSLIHETQVANRALIASFNLISDKVLEISNINSIVST
AANEQKIVTEDVAKQMEDIRYLVQENLSAMERTKQANQNISDLTTNLNDALSFFKIELTS

Specific function: May function as an environmental regulator of TCP biogenesis. Negatively regulates the synthesis of the major pilin subunit of TCP (tcpA)

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain

Homologues:

Organism=Escherichia coli, GI1788194, Length=309, Percent_Identity=30.0970873786408, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787690, Length=306, Percent_Identity=30.3921568627451, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788195, Length=337, Percent_Identity=28.486646884273, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI2367378, Length=309, Percent_Identity=28.4789644012945, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789453, Length=282, Percent_Identity=26.9503546099291, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TCPI_VIBC3 (A5F389)

Other databases:

- EMBL:   L25659
- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F389
- SMR:   A5F389
- STRING:   A5F389
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0350
- eggNOG:   COG0840
- HOGENOM:   HBG739724
- OMA:   EIANTNI
- ProtClustDB:   CLSK874121
- BioCyc:   VCHO345073:VC0395_A0350-MONOMER
- InterPro:   IPR004089
- SMART:   SM00283

Pfam domain/function: PF00015 MCPsignal

EC number: NA

Molecular weight: Translated: 69031; Mature: 69031

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIISVFLLLACIITSAFTAFFYHSKLSDQTKSISSLSSQQAQERLQSYQDSLDFYKK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNISLSVAIANSLRDKAVEELNAIALRIQENHGFIGVTFASLDGTMFTDIGTLDWNAKTL
HCCEEEEEEHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEECCCEEEEECCCCCCCHHHH
RRDWFVKTVELGTKHYTAFDIDKTTGQHVLTIATPVYVGNDIVGSVALDIAGDQIASPNG
HHHHHEEEEECCCCEEEEEECCCCCCCEEEEEECCEEECCCHHHHEEEEECCCCCCCCCC
SGMFMMTDRNFNVFASDLTHSTLIGKDLTKEKPLFKNLVSGQYVTFSDADSHWFAVSQTE
CEEEEEECCCCEEEEHHCHHHHHHCCCCCCCCCHHHHHHCCCEEEEECCCCCEEEEEEEE
IDGENKLFTIIDIQQIVQTYKRDIQLIIAGFSGFSCVMLIGLYWVLSKELSGVRQIREWI
ECCCCCEEEEEEHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLSDGQIKERRPIKFHNELDTIAQSLENLQFRLLDVVRNSHRTMNDLSIKQTDITYSIE
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEEEEE
GNTNNSQQELGLIEQVATATTQLSCTSFDVMQQAQSAELNAETAQKLIAESHDIIDSSSK
CCCCCCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
QTEMVTLSIHESQQIINQLREFSDNISSVTDVINNISDQTNLLALNAAIEAARAGEQGRG
CEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCC
FAVVADEVRSLAVKTQQSTIDIQGIILKLQEQSQLADQVMTRNVSLIHETQVANRALIAS
EEEEHHHHHHHHHHHCCCEEEEEEEEEEEHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHH
FNLISDKVLEISNINSIVSTAANEQKIVTEDVAKQMEDIRYLVQENLSAMERTKQANQNI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SDLTTNLNDALSFFKIELTS
HHHHCCHHHHHEEEEEEECC
>Mature Secondary Structure
MIKKIISVFLLLACIITSAFTAFFYHSKLSDQTKSISSLSSQQAQERLQSYQDSLDFYKK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNISLSVAIANSLRDKAVEELNAIALRIQENHGFIGVTFASLDGTMFTDIGTLDWNAKTL
HCCEEEEEEHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEECCCEEEEECCCCCCCHHHH
RRDWFVKTVELGTKHYTAFDIDKTTGQHVLTIATPVYVGNDIVGSVALDIAGDQIASPNG
HHHHHEEEEECCCCEEEEEECCCCCCCEEEEEECCEEECCCHHHHEEEEECCCCCCCCCC
SGMFMMTDRNFNVFASDLTHSTLIGKDLTKEKPLFKNLVSGQYVTFSDADSHWFAVSQTE
CEEEEEECCCCEEEEHHCHHHHHHCCCCCCCCCHHHHHHCCCEEEEECCCCCEEEEEEEE
IDGENKLFTIIDIQQIVQTYKRDIQLIIAGFSGFSCVMLIGLYWVLSKELSGVRQIREWI
ECCCCCEEEEEEHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLSDGQIKERRPIKFHNELDTIAQSLENLQFRLLDVVRNSHRTMNDLSIKQTDITYSIE
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEEEEE
GNTNNSQQELGLIEQVATATTQLSCTSFDVMQQAQSAELNAETAQKLIAESHDIIDSSSK
CCCCCCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
QTEMVTLSIHESQQIINQLREFSDNISSVTDVINNISDQTNLLALNAAIEAARAGEQGRG
CEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCC
FAVVADEVRSLAVKTQQSTIDIQGIILKLQEQSQLADQVMTRNVSLIHETQVANRALIAS
EEEEHHHHHHHHHHHCCCEEEEEEEEEEEHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHH
FNLISDKVLEISNINSIVSTAANEQKIVTEDVAKQMEDIRYLVQENLSAMERTKQANQNI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SDLTTNLNDALSFFKIELTS
HHHHCCHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8005659