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The map label for this gene is greB

Identifier: 147675721

GI number: 147675721

Start: 2443177

End: 2443662

Strand: Reverse

Name: greB

Synonym: VC0395_A2287

Alternate gene names: 147675721

Gene position: 2443662-2443177 (Counterclockwise)

Preceding gene: 147674208

Following gene: 147673571

Centisome position: 80.81

GC content: 48.77

Gene sequence:

>486_bases
GTGAAAACTAAGCTGATTACCCGAGCTGGCTATAACAAGCTCAAGCAAGAGCTCGACTATTTATGGAAAGAACAACGTCC
CGAGATCACCCAAAAAGTCTCTTGGGCGGCGAGCCTTGGCGATCGCTCAGAGAACGCTGATTACACCTACAACAAACGCC
TGCTGCGACAGATTGATCGTCGTGTACGCTTCCTGAGTAAGTTGCTGCCAGAGCTGAAAATCGTTGATTATTCACCGCAG
CAAGAAGGCAAAGTGTTTTTCGGTGCTTGGGTGGAAATTGAAAACGAAGCCGGAGAGGTGAAGAAGTTTCGCATTGTCGG
CCCCGAAGAGATTTACGGTGATGCCAAAGATTACATCTCGATTGATTCCCCGATGGCGCGCGCGCTACTCAAAAAGCAAG
TGGATGAAGAGTTTCAGGTGCACACCCCGACGGGCATCAAAGAGTGGTTTATCAACTCGATTGAGTATGAGAAAGGGGAA
TTGTGA

Upstream 100 bases:

>100_bases
CTCGCTTTTGAGAGCTTTCAGTCAAATCTTTTGCCCTGACTGGCAAAGCGTGCGAAAAATCGGTACTTTTCGCGCAACCC
TATTTTGTTGAGAATCCCGT

Downstream 100 bases:

>100_bases
GATCGTGTATTGTGGATTCGTAGCCTATCCGCTACAAAGACATAAAGATTAACAATTCTTTGCTCCCGGTGAGCGGTAAC
GATGTTACATTTTACTCGCC

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKTKLITRAGYNKLKQELDYLWKEQRPEITQKVSWAASLGDRSENADYTYNKRLLRQIDRRVRFLSKLLPELKIVDYSPQ
QEGKVFFGAWVEIENEAGEVKKFRIVGPEEIYGDAKDYISIDSPMARALLKKQVDEEFQVHTPTGIKEWFINSIEYEKGE
L

Sequences:

>Translated_161_residues
MKTKLITRAGYNKLKQELDYLWKEQRPEITQKVSWAASLGDRSENADYTYNKRLLRQIDRRVRFLSKLLPELKIVDYSPQ
QEGKVFFGAWVEIENEAGEVKKFRIVGPEEIYGDAKDYISIDSPMARALLKKQVDEEFQVHTPTGIKEWFINSIEYEKGE
L
>Mature_161_residues
MKTKLITRAGYNKLKQELDYLWKEQRPEITQKVSWAASLGDRSENADYTYNKRLLRQIDRRVRFLSKLLPELKIVDYSPQ
QEGKVFFGAWVEIENEAGEVKKFRIVGPEEIYGDAKDYISIDSPMARALLKKQVDEEFQVHTPTGIKEWFINSIEYEKGE
L

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082261, Length=158, Percent_Identity=71.5189873417722, Blast_Score=233, Evalue=5e-63,
Organism=Escherichia coli, GI87082225, Length=159, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GREB_VIBCH (Q9KNL7)

Other databases:

- EMBL:   AE003852
- PIR:   D82042
- RefSeq:   NP_232342.1
- ProteinModelPortal:   Q9KNL7
- SMR:   Q9KNL7
- GeneID:   2615543
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2715
- TIGR:   VC_2715
- HOGENOM:   HBG753387
- OMA:   KRFRIVG
- ProtClustDB:   PRK01885
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01461

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18921; Mature: 18921

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKLITRAGYNKLKQELDYLWKEQRPEITQKVSWAASLGDRSENADYTYNKRLLRQIDR
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RVRFLSKLLPELKIVDYSPQQEGKVFFGAWVEIENEAGEVKKFRIVGPEEIYGDAKDYIS
HHHHHHHHCCHHEEECCCCCCCCCEEEEEEEEECCCCCCEEEEEEECHHHHHCCCHHHEE
IDSPMARALLKKQVDEEFQVHTPTGIKEWFINSIEYEKGEL
CCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTKLITRAGYNKLKQELDYLWKEQRPEITQKVSWAASLGDRSENADYTYNKRLLRQIDR
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
RVRFLSKLLPELKIVDYSPQQEGKVFFGAWVEIENEAGEVKKFRIVGPEEIYGDAKDYIS
HHHHHHHHCCHHEEECCCCCCCCCEEEEEEEEECCCCCCEEEEEEECHHHHHCCCHHHEE
IDSPMARALLKKQVDEEFQVHTPTGIKEWFINSIEYEKGEL
CCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301