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The map label for this gene is potB [H]

Identifier: 147675686

GI number: 147675686

Start: 1083361

End: 1084221

Strand: Reverse

Name: potB [H]

Synonym: VC0395_A1038

Alternate gene names: 147675686

Gene position: 1084221-1083361 (Counterclockwise)

Preceding gene: 147673678

Following gene: 147673527

Centisome position: 35.85

GC content: 44.6

Gene sequence:

>861_bases
ATGATGAGCAAAAAGCTTAATCTACAAAATGCGATTGTTACCTTAATCGTAGGTTGGTTAGTGCTGTTTGTACTGATCCC
CAACCTGATGATTATTGGTACCAGTTTTCTGACTCGTGATGAAGCGAACCTGATTAAGTTTGCATTCACCTTTGAAAACT
ACACGCGTCTGCTTGATCCACTATACGGAAAAGTGATGCTGCACTCGTTCTACATGGCGATTGTTGCAACATTAATCTGT
CTAGTGATTGGCTATCCTTTTGCGTACATAGTGGCGAAAATGCCTGAAAAATGGCGTCCATTTATGCTGTTTTTGATTAT
TGTTCCTTTCTGGACCAACTCACTCATCCGCACCTATGGTTTAAAAATCGTACTCGGCACGCAAGGTATTCTTAATCAAG
CGCTAATGTCTCTGGGCCTTATTGATGCACCACTGCGCATTATGTTTACCGAAACAGCCGTCATGATCGGTCTGGTTTAC
ATTTTGTTGCCATTTATGATCCTTCCGCTCTATTCAGCTATCGAAAAATTGGATGGCACTTATATTGAAGCGGCGCGCGA
TCTCGGTGCCAACAAGTTTCAAACTTTAGTTCGCGTGATTTTACCGCTGACTATGCCTGGCATTATTGGTGGTTGTTTGC
TGGTTCTGCTCCCTGCCCTCGGTATGTTCTACATCGCTGACCTACTTGGCGGTGCGAAAAACCTACTGATCGGTAACGTG
ATTAAGAGCCAAGTACTCAACGCCCGTGACTGGCCATTTGGTGCCGCAACCAGTATTGCACTGACTCTGGCCATGGCGAT
CATGCTGTACGCCTACTATCGTGCAGGTAAACTTTTGAATAAGAAGGTGGAGCTAGACTAA

Upstream 100 bases:

>100_bases
ATGGTTAGCGAATTCTTTAACGAAGATGATCCAGATGTTGATCACTCTATCGGACAGAAAGTTGCCGTCACTTGGGTTGA
AAGCTGGGAGGTAGTCCTCA

Downstream 100 bases:

>100_bases
TGGGACGTTCAATTAGATTTAGCTTTATGAGTTTGGTGTACTTATTTTTGTACTTACCAATCATCGTTCTGATTGCAAAC
TCATTTAACGAAAACAAGTT

Product: spermidine/putrescine ABC transporter membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MMSKKLNLQNAIVTLIVGWLVLFVLIPNLMIIGTSFLTRDEANLIKFAFTFENYTRLLDPLYGKVMLHSFYMAIVATLIC
LVIGYPFAYIVAKMPEKWRPFMLFLIIVPFWTNSLIRTYGLKIVLGTQGILNQALMSLGLIDAPLRIMFTETAVMIGLVY
ILLPFMILPLYSAIEKLDGTYIEAARDLGANKFQTLVRVILPLTMPGIIGGCLLVLLPALGMFYIADLLGGAKNLLIGNV
IKSQVLNARDWPFGAATSIALTLAMAIMLYAYYRAGKLLNKKVELD

Sequences:

>Translated_286_residues
MMSKKLNLQNAIVTLIVGWLVLFVLIPNLMIIGTSFLTRDEANLIKFAFTFENYTRLLDPLYGKVMLHSFYMAIVATLIC
LVIGYPFAYIVAKMPEKWRPFMLFLIIVPFWTNSLIRTYGLKIVLGTQGILNQALMSLGLIDAPLRIMFTETAVMIGLVY
ILLPFMILPLYSAIEKLDGTYIEAARDLGANKFQTLVRVILPLTMPGIIGGCLLVLLPALGMFYIADLLGGAKNLLIGNV
IKSQVLNARDWPFGAATSIALTLAMAIMLYAYYRAGKLLNKKVELD
>Mature_286_residues
MMSKKLNLQNAIVTLIVGWLVLFVLIPNLMIIGTSFLTRDEANLIKFAFTFENYTRLLDPLYGKVMLHSFYMAIVATLIC
LVIGYPFAYIVAKMPEKWRPFMLFLIIVPFWTNSLIRTYGLKIVLGTQGILNQALMSLGLIDAPLRIMFTETAVMIGLVY
ILLPFMILPLYSAIEKLDGTYIEAARDLGANKFQTLVRVILPLTMPGIIGGCLLVLLPALGMFYIADLLGGAKNLLIGNV
IKSQVLNARDWPFGAATSIALTLAMAIMLYAYYRAGKLLNKKVELD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=275, Percent_Identity=70.5454545454545, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1787080, Length=219, Percent_Identity=42.9223744292237, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI1787713, Length=265, Percent_Identity=27.9245283018868, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31883; Mature: 31883

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]