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The map label for this gene is ribH [H]

Identifier: 147675653

GI number: 147675653

Start: 1976121

End: 1976642

Strand: Reverse

Name: ribH [H]

Synonym: VC0395_A1858

Alternate gene names: 147675653

Gene position: 1976642-1976121 (Counterclockwise)

Preceding gene: 147674884

Following gene: 147673228

Centisome position: 65.36

GC content: 46.55

Gene sequence:

>522_bases
GTGCTAGAATCCGGCGATTCTCACTTGATGAACAGAGTTAAAGGAAAGCTTATGAAAGTGATCGAGGGTGGTTTCCCAGC
ACCAAATGCGAAAATTGCGATTGTGATTTCTCGTTTCAACAGTTTTATCAATGAAAGTTTGCTGTCTGGTGCCATCGATA
CTCTTAAGCGTCACGGTCAGATCAGTGATGACAACATTACCGTTGTGCGTTGTCCCGGTGCGGTTGAGCTGCCTCTGGTA
GCGCAACGTGTTGCAAAAACCGGCGACTACGATGCGATTGTCTCTCTGGGTTGTGTGATCCGTGGCGGTACACCGCACTT
TGACTACGTTTGCAGTGAAATGAATAAAGGTCTGGCACAAGTGTCTCTGGAATTTAGCATTCCAGTCGCATTCGGTGTGT
TGACTGTTGATACTATCGATCAAGCTATTGAACGCGCAGGAACCAAGGCTGGTAATAAGGGTGCTGAAGCAGCACTGAGC
GCACTTGAGATGATTAATGTTCTTTCTGAAATCGATTCCTAA

Upstream 100 bases:

>100_bases
AAGTCGTTTGAGGATATTTCATGGCTGGTTTACCGCGTTTTAGCTTGATGGCAGTGCGCGGTGTAAGCCCAAATTTCTGT
TAGATATTGCTCACAAATTT

Downstream 100 bases:

>100_bases
TGGGGGCCAGTGTGAAACCAGCCGCACGTCGTAATGCACGTCAATTTGCGCTACAAGCAATTTATTCATGGCAAATTACT
AAAGAGAATGTTGCGACTAT

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MLESGDSHLMNRVKGKLMKVIEGGFPAPNAKIAIVISRFNSFINESLLSGAIDTLKRHGQISDDNITVVRCPGAVELPLV
AQRVAKTGDYDAIVSLGCVIRGGTPHFDYVCSEMNKGLAQVSLEFSIPVAFGVLTVDTIDQAIERAGTKAGNKGAEAALS
ALEMINVLSEIDS

Sequences:

>Translated_173_residues
MLESGDSHLMNRVKGKLMKVIEGGFPAPNAKIAIVISRFNSFINESLLSGAIDTLKRHGQISDDNITVVRCPGAVELPLV
AQRVAKTGDYDAIVSLGCVIRGGTPHFDYVCSEMNKGLAQVSLEFSIPVAFGVLTVDTIDQAIERAGTKAGNKGAEAALS
ALEMINVLSEIDS
>Mature_173_residues
MLESGDSHLMNRVKGKLMKVIEGGFPAPNAKIAIVISRFNSFINESLLSGAIDTLKRHGQISDDNITVVRCPGAVELPLV
AQRVAKTGDYDAIVSLGCVIRGGTPHFDYVCSEMNKGLAQVSLEFSIPVAFGVLTVDTIDQAIERAGTKAGNKGAEAALS
ALEMINVLSEIDS

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786617, Length=156, Percent_Identity=64.7435897435898, Blast_Score=210, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6324429, Length=143, Percent_Identity=32.1678321678322, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002180 [H]

Pfam domain/function: PF00885 DMRL_synthase [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 18330; Mature: 18330

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESGDSHLMNRVKGKLMKVIEGGFPAPNAKIAIVISRFNSFINESLLSGAIDTLKRHGQ
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
ISDDNITVVRCPGAVELPLVAQRVAKTGDYDAIVSLGCVIRGGTPHFDYVCSEMNKGLAQ
CCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHEEECCCCHHHHHHHHHCCCHHH
VSLEFSIPVAFGVLTVDTIDQAIERAGTKAGNKGAEAALSALEMINVLSEIDS
EEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLESGDSHLMNRVKGKLMKVIEGGFPAPNAKIAIVISRFNSFINESLLSGAIDTLKRHGQ
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
ISDDNITVVRCPGAVELPLVAQRVAKTGDYDAIVSLGCVIRGGTPHFDYVCSEMNKGLAQ
CCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHEEECCCCHHHHHHHHHCCCHHH
VSLEFSIPVAFGVLTVDTIDQAIERAGTKAGNKGAEAALSALEMINVLSEIDS
EEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA