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The map label for this gene is tnpA [H]

Identifier: 147675585

GI number: 147675585

Start: 1281773

End: 1282210

Strand: Reverse

Name: tnpA [H]

Synonym: VC0395_A1220

Alternate gene names: 147675585

Gene position: 1282210-1281773 (Counterclockwise)

Preceding gene: 147675312

Following gene: 147673215

Centisome position: 42.4

GC content: 39.04

Gene sequence:

>438_bases
ATGGGCGACTACAGAAGTTCATCACACGTCTATTGGCGTTGCAAATATCATATCGTTTGGACACCAAAATTTCGTTTTAA
GATCTTAAAAGGTAATGTTGCCAAAGAGCTAAATCGTTCGATCTACATTCTTTGTAATATGAAAGATTGTGAAGTTTTGG
AACTCAATGTTCAGCCAGGTCATGTCCACTTAGTTGCGATAATTCCGCCCAAAGTATCGATTTCGACGTTGATGGGAGTT
TTAAAGGGTAGGAGTGCAATTAGGCTATTCAACAAGTTTCCACATATCAGGAAAAAGTTATGGGGAAATCATTTTTGGGC
GCGAGGCTATTTTGTGGATACGGTAGGTGTAAATGAAGAAATCATTAGACGGTATGTACGGCATCAAGACAAAAAAGAGC
TTGAGCAAGAGCAGCAGTTAGAGTTATTGAGAGACTAA

Upstream 100 bases:

>100_bases
CTTTTAGCGGTGGTAATTGTCCCTAGGGGCTTTTGCCCGAAAATGCGCCCATGTTAGAAGACAAACTCTTATTCACCATA
AGTAAGAGGATTCAAATAAC

Downstream 100 bases:

>100_bases
CAGCGTCGTGGCCCCCTTTTAGGGGGCTTATATTAAAACCGCCTTCTAAGAAGGCGGATTTTTATTGGTAAAATGGCGGT
AGAAAGCCAGCAGTTCGCGC

Product: IS1004 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MGDYRSSSHVYWRCKYHIVWTPKFRFKILKGNVAKELNRSIYILCNMKDCEVLELNVQPGHVHLVAIIPPKVSISTLMGV
LKGRSAIRLFNKFPHIRKKLWGNHFWARGYFVDTVGVNEEIIRRYVRHQDKKELEQEQQLELLRD

Sequences:

>Translated_145_residues
MGDYRSSSHVYWRCKYHIVWTPKFRFKILKGNVAKELNRSIYILCNMKDCEVLELNVQPGHVHLVAIIPPKVSISTLMGV
LKGRSAIRLFNKFPHIRKKLWGNHFWARGYFVDTVGVNEEIIRRYVRHQDKKELEQEQQLELLRD
>Mature_144_residues
GDYRSSSHVYWRCKYHIVWTPKFRFKILKGNVAKELNRSIYILCNMKDCEVLELNVQPGHVHLVAIIPPKVSISTLMGVL
KGRSAIRLFNKFPHIRKKLWGNHFWARGYFVDTVGVNEEIIRRYVRHQDKKELEQEQQLELLRD

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: COG1943

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 17 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002686 [H]

Pfam domain/function: PF01797 Transposase_17 [H]

EC number: NA

Molecular weight: Translated: 17262; Mature: 17131

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDYRSSSHVYWRCKYHIVWTPKFRFKILKGNVAKELNRSIYILCNMKDCEVLELNVQPG
CCCCCCCCEEEEEEEEEEEECCCEEEEEECCCHHHHCCCEEEEEEECCCCEEEEEECCCC
HVHLVAIIPPKVSISTLMGVLKGRSAIRLFNKFPHIRKKLWGNHFWARGYFVDTVGVNEE
EEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCHHHHHHHCCCCEEECCEEEEECCCCHH
IIRRYVRHQDKKELEQEQQLELLRD
HHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GDYRSSSHVYWRCKYHIVWTPKFRFKILKGNVAKELNRSIYILCNMKDCEVLELNVQPG
CCCCCCCEEEEEEEEEEEECCCEEEEEECCCHHHHCCCEEEEEEECCCCEEEEEECCCC
HVHLVAIIPPKVSISTLMGVLKGRSAIRLFNKFPHIRKKLWGNHFWARGYFVDTVGVNEE
EEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCHHHHHHHCCCCEEECCEEEEECCCCHH
IIRRYVRHQDKKELEQEQQLELLRD
HHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]