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The map label for this gene is menE [H]

Identifier: 147675543

GI number: 147675543

Start: 1674948

End: 1676378

Strand: Reverse

Name: menE [H]

Synonym: VC0395_A1557

Alternate gene names: 147675543

Gene position: 1676378-1674948 (Counterclockwise)

Preceding gene: 147673492

Following gene: 147674892

Centisome position: 55.43

GC content: 52.55

Gene sequence:

>1431_bases
ATGGCGCTCGGAATCTTCCCTGTGAGCCTTTGGCAGCCTTGGCTTGAGCGTGACCCCAAGCAGGTTGCGCTGAGTCATGC
TGGGCAAACCCTGACTTGGCAAGTATTAGATAGCCAAGTAGCGCACTACGCTAAAGCGCTGCGTGAGCAAGGGGTTCGGT
CGGGGGAAGTGGTGACGCTGGTCGGGAAAAATCATCTGCACACTGTGCTGTGGTTTTTGGCTTGTACTCAAGTGGGCGCG
CTGTGCACCTTTGTCGCACCACAACCTTTAGCTCTTTTGCAGGAAAAGCTCGCCACACTCTATGCCGAGCAAAGCCCGAA
GCACCTTTGGCTAGCGCCTTCTTGCGCGCTGAGTGAAGAAGAGATTGCGGAGCTTAATGCTCATCGCCTGAGTTTGCCCG
ATGAGCGGGATGAATCCGTTGGTGGCGAGAAAGTCTCTAGCCAGTTTTCTTGTGAACAACTGGCGACGTTAATTTTCACC
TCGGGTTCGACGGGGACACCTAAAGCGGTGACTCACACTCATCAGCAACATTTGGCTTCGGCGCGCGGTTTACTGAACAA
GTTTGCTTTTACCGAGTCAGACAGTTGGTTACTGAGCTTGCCGCTGTATCATATTTCTGGTGTGGCCATTCTTTATCGTT
GGCTTGCTGTCGGAGCGACACTCAAGATCGGCACTGGCGATCTGCAAAACGATATTGCTGGCGTCACTCATGCTTCATTG
GTGCCTACTCAGCTTAAACGTCTGTTGGACAGCAGCTCTCCATTGACGCTCAAACGTGTTCTGCTCGGCGGCAGCCATAT
CCCTGTTGAACTCGCTCAAATGGCGGCAACGCGTGGTATTGATACTTGGCTCGGCTATGGCATGACCGAAGCCGCCTCCA
CCGTGACGGCAAAGCGGGTCGATGGACTTGCAGGTAATGGTGAGCTTTTGGATCATCGTGAGCTGCGCCTTGTTGATGGG
CGGATTTTCATTGCTGGGAAGACGTTGGCACAAGGCTATTATTGGCAAGGGCAGCTAGTTCCGCTCACTAATGAGCAGGG
TTGGTTTGACAGCAAAGATCTCGGTCGCTGGCAGGGCAATAACCTGTTGATTGATGGCCGAGCGGATAATCTTTTTATCT
CTGGCGGTGAAAATGTTCACTGTGAAGAAATAGAAGCGGTGTTAAACCAGCACCCTCATGTGCAAGTGGCGATCGTAATA
CCCGTTCAAGATAGTGAGTTTGGTGCGCGACCCGTCGCCGTGATTCGTAGTGAGGCCGAGTGGTGTCAAGCCATCGGTGA
TACTTGGTGCCAAGCTAAACTCGAAAAATTCAAATGGCCTATCATGTACTTTTTACTCCCCGATGCTTTGCTGGATGGGG
GAATTAAAGTCTCTCGTGCGGCGGTGAAAGCGTGGTTGGCCGCCAACCAAACGCAGTTCACTCCGCTATAA

Upstream 100 bases:

>100_bases
TACCGGGACTGGATACGATGGGATTATTCCGAACTCAGCTCGAAACGCCTTGGCCAAATAGCTCACTGCCTGTGGTTGCC
CTGCAAGAGCAGTCAATCGT

Downstream 100 bases:

>100_bases
GATTAAACCTATCCGCTGCTGGTTGAGTTTATGGTCGCGGGCGAGTCGTCAACCGTAAGTACGCCAATCCGATCACAAGG
CCAATCAGCGTCGCGCTCAT

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MALGIFPVSLWQPWLERDPKQVALSHAGQTLTWQVLDSQVAHYAKALREQGVRSGEVVTLVGKNHLHTVLWFLACTQVGA
LCTFVAPQPLALLQEKLATLYAEQSPKHLWLAPSCALSEEEIAELNAHRLSLPDERDESVGGEKVSSQFSCEQLATLIFT
SGSTGTPKAVTHTHQQHLASARGLLNKFAFTESDSWLLSLPLYHISGVAILYRWLAVGATLKIGTGDLQNDIAGVTHASL
VPTQLKRLLDSSSPLTLKRVLLGGSHIPVELAQMAATRGIDTWLGYGMTEAASTVTAKRVDGLAGNGELLDHRELRLVDG
RIFIAGKTLAQGYYWQGQLVPLTNEQGWFDSKDLGRWQGNNLLIDGRADNLFISGGENVHCEEIEAVLNQHPHVQVAIVI
PVQDSEFGARPVAVIRSEAEWCQAIGDTWCQAKLEKFKWPIMYFLLPDALLDGGIKVSRAAVKAWLAANQTQFTPL

Sequences:

>Translated_476_residues
MALGIFPVSLWQPWLERDPKQVALSHAGQTLTWQVLDSQVAHYAKALREQGVRSGEVVTLVGKNHLHTVLWFLACTQVGA
LCTFVAPQPLALLQEKLATLYAEQSPKHLWLAPSCALSEEEIAELNAHRLSLPDERDESVGGEKVSSQFSCEQLATLIFT
SGSTGTPKAVTHTHQQHLASARGLLNKFAFTESDSWLLSLPLYHISGVAILYRWLAVGATLKIGTGDLQNDIAGVTHASL
VPTQLKRLLDSSSPLTLKRVLLGGSHIPVELAQMAATRGIDTWLGYGMTEAASTVTAKRVDGLAGNGELLDHRELRLVDG
RIFIAGKTLAQGYYWQGQLVPLTNEQGWFDSKDLGRWQGNNLLIDGRADNLFISGGENVHCEEIEAVLNQHPHVQVAIVI
PVQDSEFGARPVAVIRSEAEWCQAIGDTWCQAKLEKFKWPIMYFLLPDALLDGGIKVSRAAVKAWLAANQTQFTPL
>Mature_475_residues
ALGIFPVSLWQPWLERDPKQVALSHAGQTLTWQVLDSQVAHYAKALREQGVRSGEVVTLVGKNHLHTVLWFLACTQVGAL
CTFVAPQPLALLQEKLATLYAEQSPKHLWLAPSCALSEEEIAELNAHRLSLPDERDESVGGEKVSSQFSCEQLATLIFTS
GSTGTPKAVTHTHQQHLASARGLLNKFAFTESDSWLLSLPLYHISGVAILYRWLAVGATLKIGTGDLQNDIAGVTHASLV
PTQLKRLLDSSSPLTLKRVLLGGSHIPVELAQMAATRGIDTWLGYGMTEAASTVTAKRVDGLAGNGELLDHRELRLVDGR
IFIAGKTLAQGYYWQGQLVPLTNEQGWFDSKDLGRWQGNNLLIDGRADNLFISGGENVHCEEIEAVLNQHPHVQVAIVIP
VQDSEFGARPVAVIRSEAEWCQAIGDTWCQAKLEKFKWPIMYFLLPDALLDGGIKVSRAAVKAWLAANQTQFTPL

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=467, Percent_Identity=40.8993576017131, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI145693145, Length=493, Percent_Identity=24.5436105476673, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI221142682, Length=480, Percent_Identity=21.875, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1789201, Length=266, Percent_Identity=27.0676691729323, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788107, Length=336, Percent_Identity=26.1904761904762, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=493, Percent_Identity=26.369168356998, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32563687, Length=469, Percent_Identity=25.7995735607676, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71994694, Length=320, Percent_Identity=23.75, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994690, Length=320, Percent_Identity=23.75, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994703, Length=320, Percent_Identity=23.75, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=473, Percent_Identity=23.0443974630021, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581924, Length=516, Percent_Identity=23.4496124031008, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52134; Mature: 52003

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGIFPVSLWQPWLERDPKQVALSHAGQTLTWQVLDSQVAHYAKALREQGVRSGEVVTL
CCEEEEEHHHHCHHHHCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VGKNHLHTVLWFLACTQVGALCTFVAPQPLALLQEKLATLYAEQSPKHLWLAPSCALSEE
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHH
EIAELNAHRLSLPDERDESVGGEKVSSQFSCEQLATLIFTSGSTGTPKAVTHTHQQHLAS
HHHHHCHHCCCCCCCCCCCCCCHHHHHHCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHH
ARGLLNKFAFTESDSWLLSLPLYHISGVAILYRWLAVGATLKIGTGDLQNDIAGVTHASL
HHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCHHHHH
VPTQLKRLLDSSSPLTLKRVLLGGSHIPVELAQMAATRGIDTWLGYGMTEAASTVTAKRV
HHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
DGLAGNGELLDHRELRLVDGRIFIAGKTLAQGYYWQGQLVPLTNEQGWFDSKDLGRWQGN
CCCCCCCCCCCCCCEEEECCEEEEEECHHHCCEEECCEEEEECCCCCCCCCCCCCCCCCC
NLLIDGRADNLFISGGENVHCEEIEAVLNQHPHVQVAIVIPVQDSEFGARPVAVIRSEAE
EEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHCHHH
WCQAIGDTWCQAKLEKFKWPIMYFLLPDALLDGGIKVSRAAVKAWLAANQTQFTPL
HHHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ALGIFPVSLWQPWLERDPKQVALSHAGQTLTWQVLDSQVAHYAKALREQGVRSGEVVTL
CEEEEEHHHHCHHHHCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VGKNHLHTVLWFLACTQVGALCTFVAPQPLALLQEKLATLYAEQSPKHLWLAPSCALSEE
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHH
EIAELNAHRLSLPDERDESVGGEKVSSQFSCEQLATLIFTSGSTGTPKAVTHTHQQHLAS
HHHHHCHHCCCCCCCCCCCCCCHHHHHHCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHH
ARGLLNKFAFTESDSWLLSLPLYHISGVAILYRWLAVGATLKIGTGDLQNDIAGVTHASL
HHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCHHHHH
VPTQLKRLLDSSSPLTLKRVLLGGSHIPVELAQMAATRGIDTWLGYGMTEAASTVTAKRV
HHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
DGLAGNGELLDHRELRLVDGRIFIAGKTLAQGYYWQGQLVPLTNEQGWFDSKDLGRWQGN
CCCCCCCCCCCCCCEEEECCEEEEEECHHHCCEEECCEEEEECCCCCCCCCCCCCCCCCC
NLLIDGRADNLFISGGENVHCEEIEAVLNQHPHVQVAIVIPVQDSEFGARPVAVIRSEAE
EEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHCHHH
WCQAIGDTWCQAKLEKFKWPIMYFLLPDALLDGGIKVSRAAVKAWLAANQTQFTPL
HHHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]