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The map label for this gene is groEL [H]

Identifier: 147675528

GI number: 147675528

Start: 2386492

End: 2388126

Strand: Reverse

Name: groEL [H]

Synonym: VC0395_A2237

Alternate gene names: 147675528

Gene position: 2388126-2386492 (Counterclockwise)

Preceding gene: 147675387

Following gene: 147673873

Centisome position: 78.97

GC content: 52.11

Gene sequence:

>1635_bases
ATGGCTGCTAAAGACGTACGTTTTGGTAATGACGCTCGTGTAAAAATGCTAGAAGGCGTAAACATTCTGGCCGATGCGGT
AAAAGTGACCTTAGGTCCTAAAGGCCGTAACGTAGTTCTCGACAAATCTTTCGGTGCACCAACCATTACCAAAGATGGTG
TATCAGTTGCGCGTGAAATTGAACTGGAAGACAAATTCCAGAACATGGGCGCACAAATGGTCAAAGAAGTCGCTTCTCAA
GCCAACGACGCGGCGGGTGACGGTACAACCACAGCCACCGTACTGGCTCAAGCGATTGTGAACGAAGGCCTAAAAGCGGT
CGCGGCGGGCATGAACCCAATGGATCTGAAGCGCGGGATCGATAAAGCGGTTATCGCTGCGGTAGAAGAGTTGAAAGCAC
TGTCTGTACCTTGTGCCGATACTAAAGCGATTGCCCAAGTAGGTACCATCTCTGCTAACTCAGACTCTAGCGTGGGCAAC
ATCATTGCTGAAGCGATGGAAAAAGTGGGTCGCGATGGCGTGATCACCGTTGAAGAAGGCCAAGCGCTGCAAGACGAGCT
GGATGTGGTTGAAGGTATGCAGTTTGACCGTGGCTACCTGTCACCGTACTTCATCAACAACCAAGAATCAGGCAGTGTAG
AACTGGATAACCCATTCATCCTGCTGGTGGATAAGAAAATCTCTAACATCCGCGAACTGCTGCCAGTACTGGAAGGCGTA
GCGAAAGCCTCTCGTCCACTGCTGATCGTGGCTGAAGATGTGGAAGGCGAAGCGCTGGCGACTCTGGTTGTCAACAACAT
GCGCGGCATCGTGAAAGTGGCAGCGGTTAAAGCTCCTGGCTTTGGCGATCGTCGTAAAGCGATGCTGCAAGATATCGCGA
TCTTGACTGGCGGTGTTGTGATCTCTGAAGAGATCGGTCTGGAGCTGGAAAAAGCGACTCTGGAAGACCTAGGCCAAGCG
AAACGCGTTTCTATCACCAAAGAAAACTCAACCATCATCGATGGTGCTGGCGATCAAGCCGCGATCCAAGGTCGTGTTGC
GCAGATCCGTCAACAAATCGAAGAAGCCACTTCTGACTACGACAAAGAGAAACTGCAAGAGCGCGTAGCAAAACTGGCTG
GCGGCGTTGCAGTAATCAAAGTGGGCGCAGCAACCGAAGTGGAAATGAAAGAGAAGAAAGATCGCGTAGAAGACGCGCTG
CACGCGACTCGCGCAGCGGTAGAAGAAGGCGTTGTTGCGGGTGGTGGTGTAGCCCTGATCCGCGCAGCTTCTAAACTCTC
TTCTCTGGTGGGTGACAACGAAGAGCAAAACGTGGGTATCCGTGTCGCACTACGTGCGATGGAAGCGCCACTGCGTCAAA
TCGTGAAAAACGCGGGTGACGAAGAATCAGTGGTTGCCAACAATGTGCGTGCAGGCGAAGGTAACTACGGTTACAACGCG
GCAACGGGCGTGTACGATGACATGATCGAAATGGGTATTTTGGATCCAACCAAAGTAACCCGTTCTGCTCTGCAATTCGC
AGCGTCTGTTGCGGGTCTGATGATCACCACTGAAGCGATGATCACTGAGCTGCCGAAGAAAGATGCACCAGCAATGCCAG
ACATGGGCATGGGTGGCATGGGCGGCATGATGTAA

Upstream 100 bases:

>100_bases
AAGTGCTGATCTTGGCTGAACATGACATTTTGGCAATCGTTGAATAATTGATTCTGAATCCCAACGAAATCAATAACTGA
ATTTAGAAAGGAAATGAAAA

Downstream 100 bases:

>100_bases
TCATCCCCCTTAGCAGGAAAAGCCGGAGCCCAAGCTCCGGTTTTTTTATGCCGTGGTTATGCTAAAGCATGGTTAAGATA
GGTCAGCATGACCAAGAACA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 1; Protein Cpn60 1 [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MAAKDVRFGNDARVKMLEGVNILADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFQNMGAQMVKEVASQ
ANDAAGDGTTTATVLAQAIVNEGLKAVAAGMNPMDLKRGIDKAVIAAVEELKALSVPCADTKAIAQVGTISANSDSSVGN
IIAEAMEKVGRDGVITVEEGQALQDELDVVEGMQFDRGYLSPYFINNQESGSVELDNPFILLVDKKISNIRELLPVLEGV
AKASRPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAILTGGVVISEEIGLELEKATLEDLGQA
KRVSITKENSTIIDGAGDQAAIQGRVAQIRQQIEEATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDAL
HATRAAVEEGVVAGGGVALIRAASKLSSLVGDNEEQNVGIRVALRAMEAPLRQIVKNAGDEESVVANNVRAGEGNYGYNA
ATGVYDDMIEMGILDPTKVTRSALQFAASVAGLMITTEAMITELPKKDAPAMPDMGMGGMGGMM

Sequences:

>Translated_544_residues
MAAKDVRFGNDARVKMLEGVNILADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFQNMGAQMVKEVASQ
ANDAAGDGTTTATVLAQAIVNEGLKAVAAGMNPMDLKRGIDKAVIAAVEELKALSVPCADTKAIAQVGTISANSDSSVGN
IIAEAMEKVGRDGVITVEEGQALQDELDVVEGMQFDRGYLSPYFINNQESGSVELDNPFILLVDKKISNIRELLPVLEGV
AKASRPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAILTGGVVISEEIGLELEKATLEDLGQA
KRVSITKENSTIIDGAGDQAAIQGRVAQIRQQIEEATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDAL
HATRAAVEEGVVAGGGVALIRAASKLSSLVGDNEEQNVGIRVALRAMEAPLRQIVKNAGDEESVVANNVRAGEGNYGYNA
ATGVYDDMIEMGILDPTKVTRSALQFAASVAGLMITTEAMITELPKKDAPAMPDMGMGGMGGMM
>Mature_543_residues
AAKDVRFGNDARVKMLEGVNILADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFQNMGAQMVKEVASQA
NDAAGDGTTTATVLAQAIVNEGLKAVAAGMNPMDLKRGIDKAVIAAVEELKALSVPCADTKAIAQVGTISANSDSSVGNI
IAEAMEKVGRDGVITVEEGQALQDELDVVEGMQFDRGYLSPYFINNQESGSVELDNPFILLVDKKISNIRELLPVLEGVA
KASRPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAILTGGVVISEEIGLELEKATLEDLGQAK
RVSITKENSTIIDGAGDQAAIQGRVAQIRQQIEEATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDALH
ATRAAVEEGVVAGGGVALIRAASKLSSLVGDNEEQNVGIRVALRAMEAPLRQIVKNAGDEESVVANNVRAGEGNYGYNAA
TGVYDDMIEMGILDPTKVTRSALQFAASVAGLMITTEAMITELPKKDAPAMPDMGMGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=51.9924098671727, Blast_Score=551, Evalue=1e-157,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=51.9924098671727, Blast_Score=551, Evalue=1e-157,
Organism=Homo sapiens, GI63162572, Length=543, Percent_Identity=22.0994475138122, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1790586, Length=529, Percent_Identity=86.578449905482, Blast_Score=912, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=530, Percent_Identity=52.2641509433962, Blast_Score=552, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI193210679, Length=212, Percent_Identity=52.3584905660377, Blast_Score=217, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=53.4220532319392, Blast_Score=555, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6322524, Length=140, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=53.3206831119545, Blast_Score=570, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=53.3206831119545, Blast_Score=570, Evalue=1e-163,
Organism=Drosophila melanogaster, GI45550936, Length=524, Percent_Identity=52.0992366412214, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI45550132, Length=524, Percent_Identity=52.0992366412214, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI45550935, Length=524, Percent_Identity=52.0992366412214, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI17864606, Length=542, Percent_Identity=42.2509225092251, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24584129, Length=534, Percent_Identity=38.0149812734082, Blast_Score=344, Evalue=1e-94,
Organism=Drosophila melanogaster, GI19921262, Length=534, Percent_Identity=38.0149812734082, Blast_Score=344, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24583944, Length=547, Percent_Identity=21.9378427787934, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57212; Mature: 57081

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKDVRFGNDARVKMLEGVNILADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREI
CCCCCCCCCCCCCEEHHHCHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCCCHHHHHHH
ELEDKFQNMGAQMVKEVASQANDAAGDGTTTATVLAQAIVNEGLKAVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCH
DKAVIAAVEELKALSVPCADTKAIAQVGTISANSDSSVGNIIAEAMEKVGRDGVITVEEG
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCC
QALQDELDVVEGMQFDRGYLSPYFINNQESGSVELDNPFILLVDKKISNIRELLPVLEGV
CHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEECCCEEEEECCHHHHHHHHHHHHHHH
AKASRPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAILTGGVV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHCCEE
ISEEIGLELEKATLEDLGQAKRVSITKENSTIIDGAGDQAAIQGRVAQIRQQIEEATSDY
EEHHHCCEEHHHHHHHCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
DKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDALHATRAAVEEGVVAGGGVALI
CHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RAASKLSSLVGDNEEQNVGIRVALRAMEAPLRQIVKNAGDEESVVANNVRAGEGNYGYNA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCC
ATGVYDDMIEMGILDPTKVTRSALQFAASVAGLMITTEAMITELPKKDAPAMPDMGMGGM
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GGMM
CCCC
>Mature Secondary Structure 
AAKDVRFGNDARVKMLEGVNILADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREI
CCCCCCCCCCCCEEHHHCHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCCCHHHHHHH
ELEDKFQNMGAQMVKEVASQANDAAGDGTTTATVLAQAIVNEGLKAVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCH
DKAVIAAVEELKALSVPCADTKAIAQVGTISANSDSSVGNIIAEAMEKVGRDGVITVEEG
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCC
QALQDELDVVEGMQFDRGYLSPYFINNQESGSVELDNPFILLVDKKISNIRELLPVLEGV
CHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEECCCEEEEECCHHHHHHHHHHHHHHH
AKASRPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAILTGGVV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHCCEE
ISEEIGLELEKATLEDLGQAKRVSITKENSTIIDGAGDQAAIQGRVAQIRQQIEEATSDY
EEHHHCCEEHHHHHHHCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
DKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDALHATRAAVEEGVVAGGGVALI
CHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RAASKLSSLVGDNEEQNVGIRVALRAMEAPLRQIVKNAGDEESVVANNVRAGEGNYGYNA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCC
ATGVYDDMIEMGILDPTKVTRSALQFAASVAGLMITTEAMITELPKKDAPAMPDMGMGGM
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GGMM
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA