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The map label for this gene is eda [H]

Identifier: 147675527

GI number: 147675527

Start: 2819358

End: 2819963

Strand: Reverse

Name: eda [H]

Synonym: VC0395_A2663

Alternate gene names: 147675527

Gene position: 2819963-2819358 (Counterclockwise)

Preceding gene: 147674889

Following gene: 147673813

Centisome position: 93.25

GC content: 54.95

Gene sequence:

>606_bases
ATGACCATTGAACAACGCTTACGAGCGATCAAAATTGTCCCTGTTATTGCGATTAATGATGTCGCCCACGCACTGCCACT
GGCCAAAGTGCTGGTTGAAAACGGTTTGCCTTGTGCAGAAGTCACTTTCCGCACCGCTGCGGCCGCGGAATCGATTCGCA
TCATGCGTGAGGCGTATCCAGACCTGCTGATTGGCGCAGGCACTGTACTCACGACAGCGCAAGTGGATGAAGCGATTGCG
GCGGGAGCTGATTTCATCGTGAGCCCGGGTCTGAACCCGACCACAGTGAAATACTGCCAACAGCGCAATATCGCGATCAT
TCCGGGCGTCAACAACCCAAGTTTGGTGGAACAGGCCATGGAAATGGGTCTGCGTACCCTAAAGTTCTTCCCGGCCGAGC
CATCTGGCGGCATTGCGATGCTGAAAGCGCTGAGCGCGGTGTACCCAGTGAGCTTTATGCCAACGGGGGGTATCAATCCA
AACAATGCGCAAGAGTATCTGGCGCTCAAATCGGTTGTCGCGTGTGGTGGCACTTGGATGGTACCAACTGACCTGATGGA
CAAAGGCGACTGGGACACCCTCGCCGAACTCGTACGCAACGTATAA

Upstream 100 bases:

>100_bases
AACCACTCTAAAAACGTGGACTGTGATGGAAACCCTTTTGGGCGGTATCGCCTTCCTGATTGTGGCAACACTCAGCTTTA
TTCTGTAGAGGTATGGACTC

Downstream 100 bases:

>100_bases
GCCTTTTCGGCTCGTTCCCCCACCAAAGACCAAACGCTCCGTTTGGTCTTTTTTATATTGCGCGTTAACTCGTTGAGCAG
AATGAGATATCACCTACAAG

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MTIEQRLRAIKIVPVIAINDVAHALPLAKVLVENGLPCAEVTFRTAAAAESIRIMREAYPDLLIGAGTVLTTAQVDEAIA
AGADFIVSPGLNPTTVKYCQQRNIAIIPGVNNPSLVEQAMEMGLRTLKFFPAEPSGGIAMLKALSAVYPVSFMPTGGINP
NNAQEYLALKSVVACGGTWMVPTDLMDKGDWDTLAELVRNV

Sequences:

>Translated_201_residues
MTIEQRLRAIKIVPVIAINDVAHALPLAKVLVENGLPCAEVTFRTAAAAESIRIMREAYPDLLIGAGTVLTTAQVDEAIA
AGADFIVSPGLNPTTVKYCQQRNIAIIPGVNNPSLVEQAMEMGLRTLKFFPAEPSGGIAMLKALSAVYPVSFMPTGGINP
NNAQEYLALKSVVACGGTWMVPTDLMDKGDWDTLAELVRNV
>Mature_200_residues
TIEQRLRAIKIVPVIAINDVAHALPLAKVLVENGLPCAEVTFRTAAAAESIRIMREAYPDLLIGAGTVLTTAQVDEAIAA
GADFIVSPGLNPTTVKYCQQRNIAIIPGVNNPSLVEQAMEMGLRTLKFFPAEPSGGIAMLKALSAVYPVSFMPTGGINPN
NAQEYLALKSVVACGGTWMVPTDLMDKGDWDTLAELVRNV

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=179, Percent_Identity=44.1340782122905, Blast_Score=161, Evalue=3e-41,
Organism=Escherichia coli, GI48994954, Length=148, Percent_Identity=29.7297297297297, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 21405; Mature: 21274

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEQRLRAIKIVPVIAINDVAHALPLAKVLVENGLPCAEVTFRTAAAAESIRIMREAYP
CCHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
DLLIGAGTVLTTAQVDEAIAAGADFIVSPGLNPTTVKYCQQRNIAIIPGVNNPSLVEQAM
HHEECCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHH
EMGLRTLKFFPAEPSGGIAMLKALSAVYPVSFMPTGGINPNNAQEYLALKSVVACGGTWM
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCEE
VPTDLMDKGDWDTLAELVRNV
CCHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TIEQRLRAIKIVPVIAINDVAHALPLAKVLVENGLPCAEVTFRTAAAAESIRIMREAYP
CHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
DLLIGAGTVLTTAQVDEAIAAGADFIVSPGLNPTTVKYCQQRNIAIIPGVNNPSLVEQAM
HHEECCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHH
EMGLRTLKFFPAEPSGGIAMLKALSAVYPVSFMPTGGINPNNAQEYLALKSVVACGGTWM
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCEE
VPTDLMDKGDWDTLAELVRNV
CCHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]