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The map label for this gene is flaD

Identifier: 147675484

GI number: 147675484

Start: 1850752

End: 1851885

Strand: Reverse

Name: flaD

Synonym: VC0395_A1727

Alternate gene names: 147675484

Gene position: 1851885-1850752 (Counterclockwise)

Preceding gene: 147675318

Following gene: 147674837

Centisome position: 61.24

GC content: 48.68

Gene sequence:

>1134_bases
ATGGCAGTGAATGTAAATACCAACGTAGCAGCAATGACAGCTCAACGTTATTTGACTGGTGCAACCAATGCACAACAAAC
TTCAATGGAGCGTCTATCTTCAGGCTTTAAAATCAATAGTGCTAAAGATGATGCTGCCGGCCTACAAATCTCTAACCGCT
TGAACGTACAAAGCCGCGGTCTGGATGTGGCAGTACGCAACGCGAATGATGGTATTTCAATTGCTCAAACCGCAGAAGGC
GCGATGAATGAAACTACCAACATTCTGCAACGTATGCGTGACTTGTCACTGCAATCTGCGAACGGCTCGAACTCCAAATC
TGAGCGTGTGGCAATCCAAGAAGAGATCACCGCACTGAATGATGAGCTGAACCGTATTGCAGAAACCACGTCATTCGGTG
GTAACAAGTTGCTCAACGGTACCTTCTCAACCAAGTCGTTCCAAATCGGTGCTGACAACGGTGAGGCGGTCATGCTGACC
TTGAAAGACATGCGCAGTGATAACCGCATGATGGGTGGTACCAGCTATGTCGCGGCAGAAGGCAAAGACAAAGACTGGAA
AGTACAAGCGGGCGCGAACGACATCACTTTCACGCTGAAAGACATCGACGGCAATGACCAAACCATTACCGTGAACGCTA
AAGAAGGCGATGATATCGAAGAAGTGGCGACTTACATCAACGGTCAAACCGACATGGTGAAAGCGTCTGTCAACGAGAAA
GGTCAGCTACAAATCTTTGCTGGTAACAACAAAGTCACCGGTGATGTAGCCTTCTCTGGTGGTCTAGCGGGTGCTCTGAA
CATGCAAGCGGGTACAGCAGAAACCGTTGACACTATCGATGTGACTTCAGTTGGTGGCGCGCAACAATCGGTTGCAGTTA
TCGACTCTGCGCTGAAGTATGTAGATAGTCACCGTGCTGAACTGGGTGCGTTCCAGAACCGTTTCAACCATGCTATCAGC
AACTTGGATAACATTAACGAAAACGTGAATGCTTCTAAGAGCCGTATCAAAGACACCGATTTCGCGAAAGAGACTACAGC
GCTCACCAAATCGCAAATCCTGTCTCAAGCATCAAGCTCTGTGCTGGCTCAAGCCAAACAAGCGCCAAACGCCGCACTCA
GCCTGTTGGGTTAA

Upstream 100 bases:

>100_bases
AAAAAACGCGCCGTTATAAAAGGTAACTTTGAGAGAACTACTTTGGTTTTCCGAGACGTCGGAAACCGGATACATCGGAA
AATCAATTGGAGAAATCACC

Downstream 100 bases:

>100_bases
TCCCCACACCGCCAAAAAAATCCAGCTTCGGCTGGATTTTTTATTGCAGCCAGTTTCATTAATGAGTGCCTAACTTCACG
AAAGTGGAGAGGATTTTCTT

Product: flagellin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MAVNVNTNVAAMTAQRYLTGATNAQQTSMERLSSGFKINSAKDDAAGLQISNRLNVQSRGLDVAVRNANDGISIAQTAEG
AMNETTNILQRMRDLSLQSANGSNSKSERVAIQEEITALNDELNRIAETTSFGGNKLLNGTFSTKSFQIGADNGEAVMLT
LKDMRSDNRMMGGTSYVAAEGKDKDWKVQAGANDITFTLKDIDGNDQTITVNAKEGDDIEEVATYINGQTDMVKASVNEK
GQLQIFAGNNKVTGDVAFSGGLAGALNMQAGTAETVDTIDVTSVGGAQQSVAVIDSALKYVDSHRAELGAFQNRFNHAIS
NLDNINENVNASKSRIKDTDFAKETTALTKSQILSQASSSVLAQAKQAPNAALSLLG

Sequences:

>Translated_377_residues
MAVNVNTNVAAMTAQRYLTGATNAQQTSMERLSSGFKINSAKDDAAGLQISNRLNVQSRGLDVAVRNANDGISIAQTAEG
AMNETTNILQRMRDLSLQSANGSNSKSERVAIQEEITALNDELNRIAETTSFGGNKLLNGTFSTKSFQIGADNGEAVMLT
LKDMRSDNRMMGGTSYVAAEGKDKDWKVQAGANDITFTLKDIDGNDQTITVNAKEGDDIEEVATYINGQTDMVKASVNEK
GQLQIFAGNNKVTGDVAFSGGLAGALNMQAGTAETVDTIDVTSVGGAQQSVAVIDSALKYVDSHRAELGAFQNRFNHAIS
NLDNINENVNASKSRIKDTDFAKETTALTKSQILSQASSSVLAQAKQAPNAALSLLG
>Mature_376_residues
AVNVNTNVAAMTAQRYLTGATNAQQTSMERLSSGFKINSAKDDAAGLQISNRLNVQSRGLDVAVRNANDGISIAQTAEGA
MNETTNILQRMRDLSLQSANGSNSKSERVAIQEEITALNDELNRIAETTSFGGNKLLNGTFSTKSFQIGADNGEAVMLTL
KDMRSDNRMMGGTSYVAAEGKDKDWKVQAGANDITFTLKDIDGNDQTITVNAKEGDDIEEVATYINGQTDMVKASVNEKG
QLQIFAGNNKVTGDVAFSGGLAGALNMQAGTAETVDTIDVTSVGGAQQSVAVIDSALKYVDSHRAELGAFQNRFNHAISN
LDNINENVNASKSRIKDTDFAKETTALTKSQILSQASSSVLAQAKQAPNAALSLLG

Specific function: Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. FlaD is not essential for flagellar synthesis and motility

COG id: COG1344

COG function: function code N; Flagellin and related hook-associated proteins

Gene ontology:

Cell location: Secreted. Bacterial flagellum (By similarity)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial flagellin family

Homologues:

Organism=Escherichia coli, GI1788232, Length=172, Percent_Identity=44.1860465116279, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: 200,000-400,000 (rich media) [C]

Swissprot (AC and ID): FLAD_VIBC3 (A5F6C5)

Other databases:

- EMBL:   AF007122
- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F6C5
- STRING:   A5F6C5
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1727
- eggNOG:   COG1344
- HOGENOM:   HBG758847
- OMA:   IGANENQ
- ProtClustDB:   PRK08869
- BioCyc:   VCHO345073:VC0395_A1727-MONOMER
- InterPro:   IPR001492
- InterPro:   IPR001029
- InterPro:   IPR010810
- PRINTS:   PR00207

Pfam domain/function: PF00700 Flagellin_C; PF07196 Flagellin_IN; PF00669 Flagellin_N

EC number: NA

Molecular weight: Translated: 39905; Mature: 39773

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVNVNTNVAAMTAQRYLTGATNAQQTSMERLSSGFKINSAKDDAAGLQISNRLNVQSRG
CEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEEECCCCEECCC
LDVAVRNANDGISIAQTAEGAMNETTNILQRMRDLSLQSANGSNSKSERVAIQEEITALN
CEEEEECCCCCCEEHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
DELNRIAETTSFGGNKLLNGTFSTKSFQIGADNGEAVMLTLKDMRSDNRMMGGTSYVAAE
HHHHHHHHHHCCCCCEEECCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEEEC
GKDKDWKVQAGANDITFTLKDIDGNDQTITVNAKEGDDIEEVATYINGQTDMVKASVNEK
CCCCCEEEEECCCEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCC
GQLQIFAGNNKVTGDVAFSGGLAGALNMQAGTAETVDTIDVTSVGGAQQSVAVIDSALKY
CCEEEEECCCEEEEEEEECCCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
VDSHRAELGAFQNRFNHAISNLDNINENVNASKSRIKDTDFAKETTALTKSQILSQASSS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VLAQAKQAPNAALSLLG
HHHHHHHCCCHHHHCCC
>Mature Secondary Structure 
AVNVNTNVAAMTAQRYLTGATNAQQTSMERLSSGFKINSAKDDAAGLQISNRLNVQSRG
EEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEEECCCCEECCC
LDVAVRNANDGISIAQTAEGAMNETTNILQRMRDLSLQSANGSNSKSERVAIQEEITALN
CEEEEECCCCCCEEHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
DELNRIAETTSFGGNKLLNGTFSTKSFQIGADNGEAVMLTLKDMRSDNRMMGGTSYVAAE
HHHHHHHHHHCCCCCEEECCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEEEC
GKDKDWKVQAGANDITFTLKDIDGNDQTITVNAKEGDDIEEVATYINGQTDMVKASVNEK
CCCCCEEEEECCCEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCC
GQLQIFAGNNKVTGDVAFSGGLAGALNMQAGTAETVDTIDVTSVGGAQQSVAVIDSALKY
CCEEEEECCCEEEEEEEECCCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
VDSHRAELGAFQNRFNHAISNLDNINENVNASKSRIKDTDFAKETTALTKSQILSQASSS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VLAQAKQAPNAALSLLG
HHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9440520