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The map label for this gene is mpl [H]

Identifier: 147675374

GI number: 147675374

Start: 2276410

End: 2277765

Strand: Reverse

Name: mpl [H]

Synonym: VC0395_A2121

Alternate gene names: 147675374

Gene position: 2277765-2276410 (Counterclockwise)

Preceding gene: 147675679

Following gene: 147673199

Centisome position: 75.32

GC content: 50.74

Gene sequence:

>1356_bases
ATGCACATTCATATCTTGGGAATTTGTGGCACGTTCATGGGCGGCGTTGCGATGTTAGCGCGTCAATTGGGTCATAAAGT
CACCGGCAGCGATGCCAATGTTTACCCGCCAATGAGTACCATGTTAGAGGCGCAAGGCATCGAGATCATTGAAGGTTTTG
ACCCTGCTCAACTTGAGCCCAAGCCTGATCTGGTGGTGATTGGCAACGCGATGAGTCGTGGTAACTCTTGCGTGGAATAC
GTGCTCAACCACAACCTGCGCTACACCTCTGGCCCACAGTGGCTACAAGATTTTCTGCTGCATGATCGTTGGGTGATCGC
GGTATCGGGAACGCATGGCAAAACCACCACTTCAAGCATGATTGCTTGGGTACTGGAGCATTGCGGCTATCAACCGGGTT
TCTTAGTCGGTGGTGTGTTGGGGAATTTCGGTATTTCTGCACGTTTAGGCGAAAGCATGTTTTTTGTGGTTGAAGCTGAC
GAATACGACAGTGCTTTTTTCGATAAGCGTTCTAAGTTCGTGCATTACCATCCGCGCACTTTAGTGATGAACAACCTAGA
GTTCGATCATGCAGACATTTTCGATAACTTAGAGGCGATCAAGCGCCAATTTCATCATTTGGTACGTACTGTGCCGGGTA
ATGGCCGGATTCTCGCGCCTAAGCAAGATGAAGCTTTAGCCGATGTTTTACAGCGCGGCTGTTGGAGCGAAACCGAGTTC
AGTGGTGAGCAAGGCGAGTGGCAAGCAGAAAAAATTACCGCTGATGGTTCACAGTTCCACGTTTTATTCAAAGGTGAACG
TGTAGGCCAAGTGAATTGGAGTTTGGTGGGCGATCACAACGTCAGTAATGCACTGATGGCGATTGCTGCAGCGCGTCACG
TCGGGGTTTCTCCAGAGCTGGCTTGTGAAGCGCTGGGGCTGTTTGTCAACACCAAGCGCCGCTTAGAGCTGAAAGGTGAA
ATCAATGGGGTAACGGTGTACGACGATTTTGCCCATCACCCGACGGCGATTGAACTTACGCTCGGCGGTTTACGTAACAA
GGTGGGCGATAAGAAAATCATCGCCGTACTTGAGCCGCGCTCTGCCACTATGAAGCTGGGCGTGCACAAAGAAACCTTGG
CCGCTTCGTTGCACAGCGCCGATTCGGTTTATCTTTACCAGCCGTCTAACATTCCTTGGTCGGTGGAAGAGGTCGCGAAA
CAGTGTCAACAGCCTGCGCAAGTCAGCGCCAATATGGATGAATTTGTGGCTATGATTGCCAAAGAAGCCCAAGCGGGTGA
CCAGATTTTGGTGATGAGCAACGGTGGCTTTGAAGGCATTCATGGCAAGTTGCTCGCGGCACTGCAACAGAAATAA

Upstream 100 bases:

>100_bases
ATTTCGAATTTACACCACGCTGAGCTGCTGCACTCGCTTTTGCGTAGCGGATCGGGATAATGGAGCCATTCCAATCGCAC
AGTAAAAAGGCTGGGTTTTT

Downstream 100 bases:

>100_bases
TTTAAGGTGAGCATGAACAACAATCGAACCATTACACTGGCTTGGACTGGCGCTTCCGGAGCGCCTTACGGGCTGCGCTT
ATTGCAATGCTTGTTAGCGG

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MHIHILGICGTFMGGVAMLARQLGHKVTGSDANVYPPMSTMLEAQGIEIIEGFDPAQLEPKPDLVVIGNAMSRGNSCVEY
VLNHNLRYTSGPQWLQDFLLHDRWVIAVSGTHGKTTTSSMIAWVLEHCGYQPGFLVGGVLGNFGISARLGESMFFVVEAD
EYDSAFFDKRSKFVHYHPRTLVMNNLEFDHADIFDNLEAIKRQFHHLVRTVPGNGRILAPKQDEALADVLQRGCWSETEF
SGEQGEWQAEKITADGSQFHVLFKGERVGQVNWSLVGDHNVSNALMAIAAARHVGVSPELACEALGLFVNTKRRLELKGE
INGVTVYDDFAHHPTAIELTLGGLRNKVGDKKIIAVLEPRSATMKLGVHKETLAASLHSADSVYLYQPSNIPWSVEEVAK
QCQQPAQVSANMDEFVAMIAKEAQAGDQILVMSNGGFEGIHGKLLAALQQK

Sequences:

>Translated_451_residues
MHIHILGICGTFMGGVAMLARQLGHKVTGSDANVYPPMSTMLEAQGIEIIEGFDPAQLEPKPDLVVIGNAMSRGNSCVEY
VLNHNLRYTSGPQWLQDFLLHDRWVIAVSGTHGKTTTSSMIAWVLEHCGYQPGFLVGGVLGNFGISARLGESMFFVVEAD
EYDSAFFDKRSKFVHYHPRTLVMNNLEFDHADIFDNLEAIKRQFHHLVRTVPGNGRILAPKQDEALADVLQRGCWSETEF
SGEQGEWQAEKITADGSQFHVLFKGERVGQVNWSLVGDHNVSNALMAIAAARHVGVSPELACEALGLFVNTKRRLELKGE
INGVTVYDDFAHHPTAIELTLGGLRNKVGDKKIIAVLEPRSATMKLGVHKETLAASLHSADSVYLYQPSNIPWSVEEVAK
QCQQPAQVSANMDEFVAMIAKEAQAGDQILVMSNGGFEGIHGKLLAALQQK
>Mature_451_residues
MHIHILGICGTFMGGVAMLARQLGHKVTGSDANVYPPMSTMLEAQGIEIIEGFDPAQLEPKPDLVVIGNAMSRGNSCVEY
VLNHNLRYTSGPQWLQDFLLHDRWVIAVSGTHGKTTTSSMIAWVLEHCGYQPGFLVGGVLGNFGISARLGESMFFVVEAD
EYDSAFFDKRSKFVHYHPRTLVMNNLEFDHADIFDNLEAIKRQFHHLVRTVPGNGRILAPKQDEALADVLQRGCWSETEF
SGEQGEWQAEKITADGSQFHVLFKGERVGQVNWSLVGDHNVSNALMAIAAARHVGVSPELACEALGLFVNTKRRLELKGE
INGVTVYDDFAHHPTAIELTLGGLRNKVGDKKIIAVLEPRSATMKLGVHKETLAASLHSADSVYLYQPSNIPWSVEEVAK
QCQQPAQVSANMDEFVAMIAKEAQAGDQILVMSNGGFEGIHGKLLAALQQK

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=452, Percent_Identity=68.8053097345133, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=302, Percent_Identity=29.4701986754967, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49499; Mature: 49499

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGVAMLARQLGHKVTGSDANVYPPMSTMLEAQGIEIIEGFDPAQLEP
CEEEEEEHHHHHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCC
KPDLVVIGNAMSRGNSCVEYVLNHNLRYTSGPQWLQDFLLHDRWVIAVSGTHGKTTTSSM
CCCEEEEECCCCCCCHHHHHHHHCCCEECCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
IAWVLEHCGYQPGFLVGGVLGNFGISARLGESMFFVVEADEYDSAFFDKRSKFVHYHPRT
HHHHHHHCCCCCCCEEHHHHHCCCCCEECCCEEEEEEECCCCCHHHHHCCCCEEEECCCE
LVMNNLEFDHADIFDNLEAIKRQFHHLVRTVPGNGRILAPKQDEALADVLQRGCWSETEF
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCCCCC
SGEQGEWQAEKITADGSQFHVLFKGERVGQVNWSLVGDHNVSNALMAIAAARHVGVSPEL
CCCCCCCEEEEEECCCCEEEEEEECCEECEEEEEEEECCCHHHHHHHHHHHHHCCCCHHH
ACEALGLFVNTKRRLELKGEINGVTVYDDFAHHPTAIELTLGGLRNKVGDKKIIAVLEPR
HHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCCEEEEEECHHHHCCCCCEEEEEECCC
SATMKLGVHKETLAASLHSADSVYLYQPSNIPWSVEEVAKQCQQPAQVSANMDEFVAMIA
CCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHCCCHHHHHHHHH
KEAQAGDQILVMSNGGFEGIHGKLLAALQQK
HHHCCCCEEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHIHILGICGTFMGGVAMLARQLGHKVTGSDANVYPPMSTMLEAQGIEIIEGFDPAQLEP
CEEEEEEHHHHHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCC
KPDLVVIGNAMSRGNSCVEYVLNHNLRYTSGPQWLQDFLLHDRWVIAVSGTHGKTTTSSM
CCCEEEEECCCCCCCHHHHHHHHCCCEECCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
IAWVLEHCGYQPGFLVGGVLGNFGISARLGESMFFVVEADEYDSAFFDKRSKFVHYHPRT
HHHHHHHCCCCCCCEEHHHHHCCCCCEECCCEEEEEEECCCCCHHHHHCCCCEEEECCCE
LVMNNLEFDHADIFDNLEAIKRQFHHLVRTVPGNGRILAPKQDEALADVLQRGCWSETEF
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCCCCC
SGEQGEWQAEKITADGSQFHVLFKGERVGQVNWSLVGDHNVSNALMAIAAARHVGVSPEL
CCCCCCCEEEEEECCCCEEEEEEECCEECEEEEEEEECCCHHHHHHHHHHHHHCCCCHHH
ACEALGLFVNTKRRLELKGEINGVTVYDDFAHHPTAIELTLGGLRNKVGDKKIIAVLEPR
HHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCCEEEEEECHHHHCCCCCEEEEEECCC
SATMKLGVHKETLAASLHSADSVYLYQPSNIPWSVEEVAKQCQQPAQVSANMDEFVAMIA
CCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHCCCHHHHHHHHH
KEAQAGDQILVMSNGGFEGIHGKLLAALQQK
HHHCCCCEEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]