Click here to switch to the map view.

The map label for this gene is lolA

Identifier: 147675332

GI number: 147675332

Start: 667793

End: 668389

Strand: Direct

Name: lolA

Synonym: VC0395_A0626

Alternate gene names: 147675332

Gene position: 667793-668389 (Clockwise)

Preceding gene: 147674463

Following gene: 147673167

Centisome position: 22.08

GC content: 47.74

Gene sequence:

>597_bases
ATGAACAAATGGTTAGCCCTGTTATTTTGTAGTTTCTCGGCCATTGCCGCCCCTAAAGATACCTTAAGTGAGCGTTTAGC
CATGAGTGAAGGCTTCAGCGCGACCTTTAATCAGCAAGTATTAAGCCCAGAGGGCAAAGTGATCCTGACCGGAAATGGCA
AGGTGGATATTGCTCGTCCGAGCCTATTTCGTTGGGAAACCGAAACGCCAGATGAAAACTTACTGGTCTCTGACGGTACA
ACCTTATGGCATTTCGATCCTTTCGTGGAGCAGGTCACCTTGTACCGCGCCGAAGAAGCCTTAGAGCAGACGCCTTTTGT
GCTGCTGACTCGTAATAAAGCCAGCGATTGGGATGCGTACCATGTGGAAGAAAAGGGTGATGTATTTACCCTGACGCCTA
CCGCGCTGGATTCAAACCAAGGCCGTTTCCAAATCACCATCAGTGAGAAAGGGGTGGTGCAAGGTTTTAAAGTGATTGAG
CAAGATGGTCAGCAGAGCGAATTTACCTTTAGTAAGGTGAAACAGCAGAAACCAAACGCTAGCGTATTTAACTACAAAGT
GCCGAAAGGCGTGGAAGTTGACGATCAGCGTAACTAA

Upstream 100 bases:

>100_bases
AATAGTTTATTACACATCACTGACACGTTAGAGAAGGCAAACGCTTAGGCTTTTGCTTTATTATCTTCCTCATCGCTAAA
TTGCTTGGAGCAGAGTCAGC

Downstream 100 bases:

>100_bases
ATCAGCACCAAGGAAGCGAGTCGAGTGAGTAATTACAGCTTAGATTTTTCCGGCGACGAAGATTTTCGTCCGTTAGCTGC
CCGTATGCGTCCACAAACGG

Product: outer-membrane lipoprotein carrier protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MNKWLALLFCSFSAIAAPKDTLSERLAMSEGFSATFNQQVLSPEGKVILTGNGKVDIARPSLFRWETETPDENLLVSDGT
TLWHFDPFVEQVTLYRAEEALEQTPFVLLTRNKASDWDAYHVEEKGDVFTLTPTALDSNQGRFQITISEKGVVQGFKVIE
QDGQQSEFTFSKVKQQKPNASVFNYKVPKGVEVDDQRN

Sequences:

>Translated_198_residues
MNKWLALLFCSFSAIAAPKDTLSERLAMSEGFSATFNQQVLSPEGKVILTGNGKVDIARPSLFRWETETPDENLLVSDGT
TLWHFDPFVEQVTLYRAEEALEQTPFVLLTRNKASDWDAYHVEEKGDVFTLTPTALDSNQGRFQITISEKGVVQGFKVIE
QDGQQSEFTFSKVKQQKPNASVFNYKVPKGVEVDDQRN
>Mature_198_residues
MNKWLALLFCSFSAIAAPKDTLSERLAMSEGFSATFNQQVLSPEGKVILTGNGKVDIARPSLFRWETETPDENLLVSDGT
TLWHFDPFVEQVTLYRAEEALEQTPFVLLTRNKASDWDAYHVEEKGDVFTLTPTALDSNQGRFQITISEKGVVQGFKVIE
QDGQQSEFTFSKVKQQKPNASVFNYKVPKGVEVDDQRN

Specific function: Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the l

COG id: COG2834

COG function: function code M; Outer membrane lipoprotein-sorting protein

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolA family

Homologues:

Organism=Escherichia coli, GI145693108, Length=176, Percent_Identity=40.3409090909091, Blast_Score=152, Evalue=1e-38,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): LOLA_VIBC3 (A5F2G9)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F2G9
- SMR:   A5F2G9
- STRING:   A5F2G9
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0626
- eggNOG:   COG2834
- HOGENOM:   HBG688940
- OMA:   VDIDDQR
- ProtClustDB:   PRK00031
- BioCyc:   VCHO345073:VC0395_A0626-MONOMER
- HAMAP:   MF_00240
- InterPro:   IPR004564
- InterPro:   IPR018323
- TIGRFAMs:   TIGR00547

Pfam domain/function: PF03548 LolA

EC number: NA

Molecular weight: Translated: 22301; Mature: 22301

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKWLALLFCSFSAIAAPKDTLSERLAMSEGFSATFNQQVLSPEGKVILTGNGKVDIARP
CCHHEEEEEHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCEEEECCCEEEEECC
SLFRWETETPDENLLVSDGTTLWHFDPFVEQVTLYRAEEALEQTPFVLLTRNKASDWDAY
CEEEECCCCCCCCEEEECCCEEEEECHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEE
HVEEKGDVFTLTPTALDSNQGRFQITISEKGVVQGFKVIEQDGQQSEFTFSKVKQQKPNA
EECCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
SVFNYKVPKGVEVDDQRN
CEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNKWLALLFCSFSAIAAPKDTLSERLAMSEGFSATFNQQVLSPEGKVILTGNGKVDIARP
CCHHEEEEEHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCEEEECCCEEEEECC
SLFRWETETPDENLLVSDGTTLWHFDPFVEQVTLYRAEEALEQTPFVLLTRNKASDWDAY
CEEEECCCCCCCCEEEECCCEEEEECHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEE
HVEEKGDVFTLTPTALDSNQGRFQITISEKGVVQGFKVIEQDGQQSEFTFSKVKQQKPNA
EECCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
SVFNYKVPKGVEVDDQRN
CEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA