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The map label for this gene is rlmF [H]

Identifier: 147675312

GI number: 147675312

Start: 1282254

End: 1283189

Strand: Reverse

Name: rlmF [H]

Synonym: VC0395_A1221

Alternate gene names: 147675312

Gene position: 1283189-1282254 (Counterclockwise)

Preceding gene: 147673336

Following gene: 147675585

Centisome position: 42.43

GC content: 47.65

Gene sequence:

>936_bases
ATGAACACTCCACTTAAGCCAAAACATGGGCAAAAAACAAATCAAAAACCGAAAGCCAATAAGCCAGTTGTGAAAAAGCA
GCAAACTAAGCAGCCGCCAACCCACAAAGTACAAGGTGAGGAAGTCGCTGCCGTTAAATCTGGGTTACATCCACGCAATC
GTCATCGCGGGCAGTATGATTTTCCGGCACTGATTAAGGTCGTGCCAGAGTTGCAATCCCATGTTATGAAAAATCCTAAA
GGGCAATGGACGATTAATTTTGCCGATCCGATCGCGGTCAAGTTACTCAATAAAGCGCTTTTGGCTCTGCATTACGGTGT
CACCTATTGGGATATTCCAGAGGGCTTCTTATGCCCGCCCATTCCGGGTCGTGCGGATTACATTCATCGCGTGGCCGATC
TGCTGCTTAAGGAGAATCCAAAATTAAACCCTTCGCAAGTCACAGCACTGGATATTGGCGTGGGCGCAAATTGTATCTAT
CCGATTGTTGGAGTGACGGAATACGGATGGTCATGGGTTGGCAGTGATGTCGATCCTGTCTCAATACAACAAGCGAGTTT
GATTGTTCAAAGCAACTCGAAGCTGCAAGGCCATATTGAATTTCGCCTGCAAAAGAACAGCCAACATATTTTTAACGGCA
TTATTGGGGCGAATGAGCGTTATACGCTGACGACTTGCAACCCGCCTTTTCATGCATCCTTAGCTGACGCTCAGCAAGGA
ACCCAGCGAAAGTTGACCAACTTACAGGCAAATCAGCGCAAGAAAGGCCGCTTAGCAACGCCAACGTTAAGCCACTCAAG
GTTAAATTTTGGTGGTCAGAAAGCCGAGTTATGGTGTCCAGGAGGAGAAGCGGCTTTCATTGTCATCCCTAAACCACCGC
TTTTAGCGGTGGTAATTGTCCCTAGGGGCTTTTGCCCGAAAATGCGCCCATGTTAG

Upstream 100 bases:

>100_bases
CGTTTTATCAGCTCTGTTGCCATATGGAACCGAATTATCATCAGCTTGAACTGGATGCACGGTTATACATGATGGTGCTA
GAGCTCAAGGCAGGCAAAGC

Downstream 100 bases:

>100_bases
AAGACAAACTCTTATTCACCATAAGTAAGAGGATTCAAATAACATGGGCGACTACAGAAGTTCATCACACGTCTATTGGC
GTTGCAAATATCATATCGTT

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNTPLKPKHGQKTNQKPKANKPVVKKQQTKQPPTHKVQGEEVAAVKSGLHPRNRHRGQYDFPALIKVVPELQSHVMKNPK
GQWTINFADPIAVKLLNKALLALHYGVTYWDIPEGFLCPPIPGRADYIHRVADLLLKENPKLNPSQVTALDIGVGANCIY
PIVGVTEYGWSWVGSDVDPVSIQQASLIVQSNSKLQGHIEFRLQKNSQHIFNGIIGANERYTLTTCNPPFHASLADAQQG
TQRKLTNLQANQRKKGRLATPTLSHSRLNFGGQKAELWCPGGEAAFIVIPKPPLLAVVIVPRGFCPKMRPC

Sequences:

>Translated_311_residues
MNTPLKPKHGQKTNQKPKANKPVVKKQQTKQPPTHKVQGEEVAAVKSGLHPRNRHRGQYDFPALIKVVPELQSHVMKNPK
GQWTINFADPIAVKLLNKALLALHYGVTYWDIPEGFLCPPIPGRADYIHRVADLLLKENPKLNPSQVTALDIGVGANCIY
PIVGVTEYGWSWVGSDVDPVSIQQASLIVQSNSKLQGHIEFRLQKNSQHIFNGIIGANERYTLTTCNPPFHASLADAQQG
TQRKLTNLQANQRKKGRLATPTLSHSRLNFGGQKAELWCPGGEAAFIVIPKPPLLAVVIVPRGFCPKMRPC
>Mature_311_residues
MNTPLKPKHGQKTNQKPKANKPVVKKQQTKQPPTHKVQGEEVAAVKSGLHPRNRHRGQYDFPALIKVVPELQSHVMKNPK
GQWTINFADPIAVKLLNKALLALHYGVTYWDIPEGFLCPPIPGRADYIHRVADLLLKENPKLNPSQVTALDIGVGANCIY
PIVGVTEYGWSWVGSDVDPVSIQQASLIVQSNSKLQGHIEFRLQKNSQHIFNGIIGANERYTLTTCNPPFHASLADAQQG
TQRKLTNLQANQRKKGRLATPTLSHSRLNFGGQKAELWCPGGEAAFIVIPKPPLLAVVIVPRGFCPKMRPC

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=246, Percent_Identity=31.3008130081301, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI87081786, Length=246, Percent_Identity=51.6260162601626, Blast_Score=260, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI32564931, Length=253, Percent_Identity=26.8774703557312, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32564933, Length=253, Percent_Identity=24.1106719367589, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281363415, Length=244, Percent_Identity=28.2786885245902, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922302, Length=244, Percent_Identity=28.2786885245902, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 34397; Mature: 34397

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPLKPKHGQKTNQKPKANKPVVKKQQTKQPPTHKVQGEEVAAVKSGLHPRNRHRGQYD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
FPALIKVVPELQSHVMKNPKGQWTINFADPIAVKLLNKALLALHYGVTYWDIPEGFLCPP
HHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
IPGRADYIHRVADLLLKENPKLNPSQVTALDIGVGANCIYPIVGVTEYGWSWVGSDVDPV
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEHEEECCCCCCEECCCCCCCC
SIQQASLIVQSNSKLQGHIEFRLQKNSQHIFNGIIGANERYTLTTCNPPFHASLADAQQG
EEEEEEEEEECCCCEEEEEEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHH
TQRKLTNLQANQRKKGRLATPTLSHSRLNFGGQKAELWCPGGEAAFIVIPKPPLLAVVIV
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCEEEEEEE
PRGFCPKMRPC
CCCCCCCCCCC
>Mature Secondary Structure
MNTPLKPKHGQKTNQKPKANKPVVKKQQTKQPPTHKVQGEEVAAVKSGLHPRNRHRGQYD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
FPALIKVVPELQSHVMKNPKGQWTINFADPIAVKLLNKALLALHYGVTYWDIPEGFLCPP
HHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
IPGRADYIHRVADLLLKENPKLNPSQVTALDIGVGANCIYPIVGVTEYGWSWVGSDVDPV
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEHEEECCCCCCEECCCCCCCC
SIQQASLIVQSNSKLQGHIEFRLQKNSQHIFNGIIGANERYTLTTCNPPFHASLADAQQG
EEEEEEEEEECCCCEEEEEEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHH
TQRKLTNLQANQRKKGRLATPTLSHSRLNFGGQKAELWCPGGEAAFIVIPKPPLLAVVIV
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCEEEEEEE
PRGFCPKMRPC
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA