Click here to switch to the map view.

The map label for this gene is trmD [H]

Identifier: 147675311

GI number: 147675311

Start: 87929

End: 88672

Strand: Direct

Name: trmD [H]

Synonym: VC0395_A0097

Alternate gene names: 147675311

Gene position: 87929-88672 (Clockwise)

Preceding gene: 147674058

Following gene: 147674723

Centisome position: 2.91

GC content: 49.6

Gene sequence:

>744_bases
ATGTGGGTTGGCATCGTTAGCCTATTTCCTGAAATGTTCCGCAGCGTTACCGATTTTGGAGTAACAGGTCAGGCGGTTAA
AAAAGGGTTGTTGTCAGTTGAAGCGTGGAATCCTCGTGATTTCGCTCATGACAAACGTCGCACTGTCGATGATAAACCCT
ACGGTGGCGGTCCCGGCATGTTGATGATGGTGCAACCTTTGCGCGATGCCATCCATGCTGCGAAACAAGCGTCACCGGGT
AAGACGAAAGTCATTTACCTCTCTCCGCAAGGTCGAAAACTCGACCAACAAGGTGTGGAAGAGCTGGCGCAAAACCAGAA
TCTGATTCTGATTTGCGGTCGCTATGAAGGGGTAGATGAACGCATTATTGAATCTGAAGTTGACGAAGAATGGTCAATTG
GTGATTTTGTAATGACGGGTGGGGAACTCCCAGCCATGACGTTGATTGATTCTGTCTCGCGGTTCATTCCGGGAGTATTA
GGGGATTTCGCGTCAGCAGAAGAAGACTCTTTTGCCAATGGTTTGCTGGATTGCCCTCACTACACGCGTCCCGAGGTATT
GGACGGAAAAGAAGTGCCAGCGGTACTGAAATCCGGCAATCATGAGGACATTCGTCGTTGGCGACTCAAGCAGTCGCTTG
GCCGTACCTGGCTTAGAAGACCGGAGCTCCTGGAAAACCTAGCTCTGACTGACGAACAGGAACAATTACTGACCGAGTAC
ATTAAAGAGACTCGTCATCAGTAA

Upstream 100 bases:

>100_bases
TGAAGAGCAAGTGATCAAAGTTGTTGATCGCCAAGCTCAACGGATCGAAGTTGACTGGGATCCTGCGTTTTAACTCTTAA
AATCGAATAAGCGAGGAAAC

Downstream 100 bases:

>100_bases
CCTATTAAATTTAGTATCAGTTTATTCTAGGAATTTAGACAATGAGTAACATCATCAAGGCTCTAGAACAAGAGCAAATG
AAACAAGACCTGCCTCAATT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MWVGIVSLFPEMFRSVTDFGVTGQAVKKGLLSVEAWNPRDFAHDKRRTVDDKPYGGGPGMLMMVQPLRDAIHAAKQASPG
KTKVIYLSPQGRKLDQQGVEELAQNQNLILICGRYEGVDERIIESEVDEEWSIGDFVMTGGELPAMTLIDSVSRFIPGVL
GDFASAEEDSFANGLLDCPHYTRPEVLDGKEVPAVLKSGNHEDIRRWRLKQSLGRTWLRRPELLENLALTDEQEQLLTEY
IKETRHQ

Sequences:

>Translated_247_residues
MWVGIVSLFPEMFRSVTDFGVTGQAVKKGLLSVEAWNPRDFAHDKRRTVDDKPYGGGPGMLMMVQPLRDAIHAAKQASPG
KTKVIYLSPQGRKLDQQGVEELAQNQNLILICGRYEGVDERIIESEVDEEWSIGDFVMTGGELPAMTLIDSVSRFIPGVL
GDFASAEEDSFANGLLDCPHYTRPEVLDGKEVPAVLKSGNHEDIRRWRLKQSLGRTWLRRPELLENLALTDEQEQLLTEY
IKETRHQ
>Mature_247_residues
MWVGIVSLFPEMFRSVTDFGVTGQAVKKGLLSVEAWNPRDFAHDKRRTVDDKPYGGGPGMLMMVQPLRDAIHAAKQASPG
KTKVIYLSPQGRKLDQQGVEELAQNQNLILICGRYEGVDERIIESEVDEEWSIGDFVMTGGELPAMTLIDSVSRFIPGVL
GDFASAEEDSFANGLLDCPHYTRPEVLDGKEVPAVLKSGNHEDIRRWRLKQSLGRTWLRRPELLENLALTDEQEQLLTEY
IKETRHQ

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=247, Percent_Identity=78.1376518218623, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27807; Mature: 27807

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWVGIVSLFPEMFRSVTDFGVTGQAVKKGLLSVEAWNPRDFAHDKRRTVDDKPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCCCCCCH
LMMVQPLRDAIHAAKQASPGKTKVIYLSPQGRKLDQQGVEELAQNQNLILICGRYEGVDE
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHH
RIIESEVDEEWSIGDFVMTGGELPAMTLIDSVSRFIPGVLGDFASAEEDSFANGLLDCPH
HHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
YTRPEVLDGKEVPAVLKSGNHEDIRRWRLKQSLGRTWLRRPELLENLALTDEQEQLLTEY
CCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHH
IKETRHQ
HHHHCCC
>Mature Secondary Structure
MWVGIVSLFPEMFRSVTDFGVTGQAVKKGLLSVEAWNPRDFAHDKRRTVDDKPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCCCCCCH
LMMVQPLRDAIHAAKQASPGKTKVIYLSPQGRKLDQQGVEELAQNQNLILICGRYEGVDE
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHH
RIIESEVDEEWSIGDFVMTGGELPAMTLIDSVSRFIPGVLGDFASAEEDSFANGLLDCPH
HHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
YTRPEVLDGKEVPAVLKSGNHEDIRRWRLKQSLGRTWLRRPELLENLALTDEQEQLLTEY
CCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHH
IKETRHQ
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA