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The map label for this gene is mlaE [H]

Identifier: 147675288

GI number: 147675288

Start: 2258533

End: 2259309

Strand: Reverse

Name: mlaE [H]

Synonym: VC0395_A2101

Alternate gene names: 147675288

Gene position: 2259309-2258533 (Counterclockwise)

Preceding gene: 147674625

Following gene: 147673144

Centisome position: 74.71

GC content: 53.8

Gene sequence:

>777_bases
ATGCGATGGGTCAGCTCTGTTGGGCAACGCACGCTGGCGGTCAGTGAAACCTTTGGCCGCGCAAGCTTAATGCTGTTTGG
TGCACTGGTAGGGCGTCCGCAGCCGATTCGCCATTTTCCGCTCCTCGTTCGCCAGTTATACAGCATTGGTGTGCAGTCAC
TGGCGATCATTATCGTCTCTGGGCTCTTTATTGGCATGGTGCTCAGCTTACAAGGCTATGTGATTTTGGTCGATTACGGC
GCAGAAACCAGCCTAGGCCAGATGGTGGCGTTATCATTGCTGCGTGAATTAGGCCCAGTGGTTACGGCACTGCTGTTTGC
CGGACGAGCGGGCTCTGCCTTAACCGCCGAAATTGGCTTGATGAAAGCCACCGAGCAGCTGTCTAGCCTTGAAATGATGG
CGGTCGATCCACTGAAACGCGTTATTGCGCCACGCTTTTGGGCGGGAGTCATCTCCATGCCGCTGCTGGCGATGATTTTC
ATGGCGGTCGGCATTTGGGGCGGCCAATTGGTTGGAGTGGATTGGAAAGGCATTGATCACGGCAGTTTCTGGTCAGCCAT
GCAAGCTTCGGTTGAATTGGGCCAAGATATCGGTAACAGCACCATCAAATGTGTGGTGTTTGCGTTTACCGTGACTTGGA
TTGCTCTGTTTAACGGTTATGACGCGATCCCGACTTCAGAAGGGATCAGCCGAGCGACCACTCGCACCGTAGTGCACTCA
TCACTGGCCGTACTTGGGCTGGATTTTGTTCTAACCGCATTGATGTTTGGGAACTGA

Upstream 100 bases:

>100_bases
CGCGTACAACAATTTTTATGCGGCGATGCAGATGGCCCTGTGCCATTTCGTTTTCCTGCGCAGCCGATAGAACAGGAGCT
GTTTAGTGCTAAATGATGTG

Downstream 100 bases:

>100_bases
TCATGCAACAAACACGAAAAATTGAATTTTGGGTTGGCAGCTTTGTCATCGCGGGAATTTGCGCGATTTTAGTGATGATT
TTTCAGGTCGCTGACGTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MRWVSSVGQRTLAVSETFGRASLMLFGALVGRPQPIRHFPLLVRQLYSIGVQSLAIIIVSGLFIGMVLSLQGYVILVDYG
AETSLGQMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLKRVIAPRFWAGVISMPLLAMIF
MAVGIWGGQLVGVDWKGIDHGSFWSAMQASVELGQDIGNSTIKCVVFAFTVTWIALFNGYDAIPTSEGISRATTRTVVHS
SLAVLGLDFVLTALMFGN

Sequences:

>Translated_258_residues
MRWVSSVGQRTLAVSETFGRASLMLFGALVGRPQPIRHFPLLVRQLYSIGVQSLAIIIVSGLFIGMVLSLQGYVILVDYG
AETSLGQMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLKRVIAPRFWAGVISMPLLAMIF
MAVGIWGGQLVGVDWKGIDHGSFWSAMQASVELGQDIGNSTIKCVVFAFTVTWIALFNGYDAIPTSEGISRATTRTVVHS
SLAVLGLDFVLTALMFGN
>Mature_258_residues
MRWVSSVGQRTLAVSETFGRASLMLFGALVGRPQPIRHFPLLVRQLYSIGVQSLAIIIVSGLFIGMVLSLQGYVILVDYG
AETSLGQMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLKRVIAPRFWAGVISMPLLAMIF
MAVGIWGGQLVGVDWKGIDHGSFWSAMQASVELGQDIGNSTIKCVVFAFTVTWIALFNGYDAIPTSEGISRATTRTVVHS
SLAVLGLDFVLTALMFGN

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=255, Percent_Identity=76.078431372549, Blast_Score=387, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27626; Mature: 27626

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWVSSVGQRTLAVSETFGRASLMLFGALVGRPQPIRHFPLLVRQLYSIGVQSLAIIIVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GLFIGMVLSLQGYVILVDYGAETSLGQMVALSLLRELGPVVTALLFAGRAGSALTAEIGL
HHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
MKATEQLSSLEMMAVDPLKRVIAPRFWAGVISMPLLAMIFMAVGIWGGQLVGVDWKGIDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GSFWSAMQASVELGQDIGNSTIKCVVFAFTVTWIALFNGYDAIPTSEGISRATTRTVVHS
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
SLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRWVSSVGQRTLAVSETFGRASLMLFGALVGRPQPIRHFPLLVRQLYSIGVQSLAIIIVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GLFIGMVLSLQGYVILVDYGAETSLGQMVALSLLRELGPVVTALLFAGRAGSALTAEIGL
HHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
MKATEQLSSLEMMAVDPLKRVIAPRFWAGVISMPLLAMIFMAVGIWGGQLVGVDWKGIDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GSFWSAMQASVELGQDIGNSTIKCVVFAFTVTWIALFNGYDAIPTSEGISRATTRTVVHS
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
SLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]