Click here to switch to the map view.

The map label for this gene is ruvA

Identifier: 147675231

GI number: 147675231

Start: 1539058

End: 1539672

Strand: Reverse

Name: ruvA

Synonym: VC0395_A1437

Alternate gene names: 147675231

Gene position: 1539672-1539058 (Counterclockwise)

Preceding gene: 147673791

Following gene: 147673267

Centisome position: 50.91

GC content: 48.29

Gene sequence:

>615_bases
GTGATTGGACGTCTTCGCGGTACGCTGATTGAAAAGTTACCTCCTCAAATTTTGATTGAGGTCGGTGGTATTGGCTATGA
AGTGCAAATGCCGATGAGCTGTATTTATGAACTGCCGAATATTGGGGAAGAAGCCATCATTTATACCCACTTTGTAGTTC
GAGAAGATGCACAGCTACTTTATGGCTTTAATACCGTTAGCGAACGCGCTCTGTTTCGTGAAGTAATCAAAGCCAATGGC
GTAGGGCCGAAAATGGGGCTTGCGATCCTCTCTGGCATGACAGCCAATCAGTTTGTGACTTGTGTGGAAAAAGAAGATAT
CTCTACCCTCATTAAATTACCGGGAGTCGGGAAGAAGACGGCTGAGCGATTGGTGGTGGAAATGAAAGATCGCCTGAAAG
GATGGGGAGCGGGTGACCTCTTTACACCTGCAACCGATGCGGCCCCTGTCGATTCTACGCCCGTGATTGCTCAAAATGCT
CAGGAAGAAGCCATGAGCGCGCTATTGGCGCTAGGTTACAAGCCCCCTCAAGCTTCCAAAGCGGTATCGCAAGTGGCTAA
AGCAGGAATGAGCAGTGAAGAGTTGATTCGTGAAGCGCTCAAATCTATGGTGTAA

Upstream 100 bases:

>100_bases
TTAAATGTAGCTTGATGCTACGAGCATTTTACTGGCTATCCATCCAGTTATTGATTATCATCCAGAGCATCTTTCCTCCC
TCTATTATAAGGAAACACAA

Downstream 100 bases:

>100_bases
CGATTCACGTTTTGCCGTCGTTAAAAACAGTCGCCATTAAGCCAATAGAGAAAATATGATAGAAGCCGATCGTTTAATTG
CTCCAATCAGCAACCACTTC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MIGRLRGTLIEKLPPQILIEVGGIGYEVQMPMSCIYELPNIGEEAIIYTHFVVREDAQLLYGFNTVSERALFREVIKANG
VGPKMGLAILSGMTANQFVTCVEKEDISTLIKLPGVGKKTAERLVVEMKDRLKGWGAGDLFTPATDAAPVDSTPVIAQNA
QEEAMSALLALGYKPPQASKAVSQVAKAGMSSEELIREALKSMV

Sequences:

>Translated_204_residues
MIGRLRGTLIEKLPPQILIEVGGIGYEVQMPMSCIYELPNIGEEAIIYTHFVVREDAQLLYGFNTVSERALFREVIKANG
VGPKMGLAILSGMTANQFVTCVEKEDISTLIKLPGVGKKTAERLVVEMKDRLKGWGAGDLFTPATDAAPVDSTPVIAQNA
QEEAMSALLALGYKPPQASKAVSQVAKAGMSSEELIREALKSMV
>Mature_204_residues
MIGRLRGTLIEKLPPQILIEVGGIGYEVQMPMSCIYELPNIGEEAIIYTHFVVREDAQLLYGFNTVSERALFREVIKANG
VGPKMGLAILSGMTANQFVTCVEKEDISTLIKLPGVGKKTAERLVVEMKDRLKGWGAGDLFTPATDAAPVDSTPVIAQNA
QEEAMSALLALGYKPPQASKAVSQVAKAGMSSEELIREALKSMV

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=202, Percent_Identity=64.8514851485149, Blast_Score=277, Evalue=3e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_VIBC3 (A5F761)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F761
- SMR:   A5F761
- STRING:   A5F761
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1437
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- BioCyc:   VCHO345073:VC0395_A1437-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 22009; Mature: 22009

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRLRGTLIEKLPPQILIEVGGIGYEVQMPMSCIYELPNIGEEAIIYTHFVVREDAQLL
CCCCHHHHHHHHCCHHHEEEECCCCEEEECHHHHHHHCCCCCCCCEEHHHHHHHCCHHHH
YGFNTVSERALFREVIKANGVGPKMGLAILSGMTANQFVTCVEKEDISTLIKLPGVGKKT
HCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
AERLVVEMKDRLKGWGAGDLFTPATDAAPVDSTPVIAQNAQEEAMSALLALGYKPPQASK
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHH
AVSQVAKAGMSSEELIREALKSMV
HHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIGRLRGTLIEKLPPQILIEVGGIGYEVQMPMSCIYELPNIGEEAIIYTHFVVREDAQLL
CCCCHHHHHHHHCCHHHEEEECCCCEEEECHHHHHHHCCCCCCCCEEHHHHHHHCCHHHH
YGFNTVSERALFREVIKANGVGPKMGLAILSGMTANQFVTCVEKEDISTLIKLPGVGKKT
HCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
AERLVVEMKDRLKGWGAGDLFTPATDAAPVDSTPVIAQNAQEEAMSALLALGYKPPQASK
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHH
AVSQVAKAGMSSEELIREALKSMV
HHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA