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The map label for this gene is epsF

Identifier: 147675103

GI number: 147675103

Start: 2456314

End: 2457534

Strand: Reverse

Name: epsF

Synonym: VC0395_A2304

Alternate gene names: 147675103

Gene position: 2457534-2456314 (Counterclockwise)

Preceding gene: 147673384

Following gene: 147674515

Centisome position: 81.27

GC content: 53.24

Gene sequence:

>1221_bases
ATGGCCGCGTTTGAATACAAAGCGCTGGATGCCAAGGGACGCCATAAAAAAGGCGTGATTGAGGGCGATAATGCACGTCA
GGTGCGTCAGCGCCTGAAAGAGCAAAGCCTAGTGCCGATGGAGGTGGTGGAGACTCAAGTCAAAGCCGCGCGCAGTCGCA
GCCAAGGTTTTGCGTTTAAGCGTGGGATCAGTACGCCGGATCTGGCGCTGATCACTCGCCAATTGGCGACTTTAGTTCAA
TCCGGTATGCCACTGGAAGAGTGTTTACGCGCGGTTGCCGAGCAGTCGGAAAAACCGCGGATTCGCACCATGTTGGTGGC
GGTGCGCGCTAAAGTGACCGAAGGTTACACCCTCTCTGATAGTCTTGGCGATTATCCGCACGTGTTTGATGAGCTGTTTC
GTTCTATGGTTGCGGCGGGCGAAAAATCTGGCCACCTCGATTCCGTTCTCGAACGTTTAGCTGACTACGCCGAAAACCGC
CAGAAAATGCGCTCTAAACTGCAACAGGCCATGATTTACCCTGTGGTGCTGGTGGTGTTTGCGGTCGGTATCGTGGCGTT
TTTGTTGGCAGCGGTAGTGCCGAAAATCGTGGGTCAGTTTGTGCAAATGGGGCAAGCCTTGCCGGCATCGACTCAGTTTC
TGCTTGATGCCAGCGATTTCCTGCAACATTGGGGGATTTCGCTGCTGGTCGGTCTCTTGATGCTGATTTATCTGGTGCGC
TGGTTGCTGACCAAGCCCGATATTCGTTTGCGTTGGGATCGCCGAGTGATTTCCTTGCCTGTGATTGGCAAGATTGCACG
CGGTCTGAATACTGCGCGCTTTGCGCGTACGCTGTCGATCTGTACCTCAAGTGCAATCCCGATTCTGGATGGTATGCGCG
TTGCGGTGGATGTGATGACCAATCAGTTTGTGAAACAGCAAGTGTTGGCTGCGGCCGAAAACGTACGCGAAGGCTCTAGC
CTGCGCAAAGCGCTAGAGCAGACCAAGCTCTTTCCTCCCATGATGCTGCACATGATTGCCAGTGGTGAGCAGAGTGGAGA
ATTGGAAGGCATGTTGACGCGCGCTGCGGATAACCAAGACAACAGTTTTGAATCAACGGTCAACATCGCGCTTGGCATTT
TTACCCCGGCCTTGATTGCCTTGATGGCGGGGATGGTGCTGTTTATTGTGATGGCGACCCTGATGCCGATTTTGGAAATG
AATAACTTAATGAGTCGTTAA

Upstream 100 bases:

>100_bases
TTCGCGCGACCACGCCGAGCATTCGTGATGATGGTCTAGATAAGGTGCGCCAAGGCATTACTTCGCTAGAAGAAGTGATG
CGGGTGACTAAGGAGTCCTA

Downstream 100 bases:

>100_bases
GTCGTCACGCGCGGCGAAAACGACATAGTGTGGAGTAACTATGAAAAAAATGCGTAAACAAACGGGCTTTACCCTGCTCG
AAGTAATGGTGGTTGTGGTG

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MAAFEYKALDAKGRHKKGVIEGDNARQVRQRLKEQSLVPMEVVETQVKAARSRSQGFAFKRGISTPDLALITRQLATLVQ
SGMPLEECLRAVAEQSEKPRIRTMLVAVRAKVTEGYTLSDSLGDYPHVFDELFRSMVAAGEKSGHLDSVLERLADYAENR
QKMRSKLQQAMIYPVVLVVFAVGIVAFLLAAVVPKIVGQFVQMGQALPASTQFLLDASDFLQHWGISLLVGLLMLIYLVR
WLLTKPDIRLRWDRRVISLPVIGKIARGLNTARFARTLSICTSSAIPILDGMRVAVDVMTNQFVKQQVLAAAENVREGSS
LRKALEQTKLFPPMMLHMIASGEQSGELEGMLTRAADNQDNSFESTVNIALGIFTPALIALMAGMVLFIVMATLMPILEM
NNLMSR

Sequences:

>Translated_406_residues
MAAFEYKALDAKGRHKKGVIEGDNARQVRQRLKEQSLVPMEVVETQVKAARSRSQGFAFKRGISTPDLALITRQLATLVQ
SGMPLEECLRAVAEQSEKPRIRTMLVAVRAKVTEGYTLSDSLGDYPHVFDELFRSMVAAGEKSGHLDSVLERLADYAENR
QKMRSKLQQAMIYPVVLVVFAVGIVAFLLAAVVPKIVGQFVQMGQALPASTQFLLDASDFLQHWGISLLVGLLMLIYLVR
WLLTKPDIRLRWDRRVISLPVIGKIARGLNTARFARTLSICTSSAIPILDGMRVAVDVMTNQFVKQQVLAAAENVREGSS
LRKALEQTKLFPPMMLHMIASGEQSGELEGMLTRAADNQDNSFESTVNIALGIFTPALIALMAGMVLFIVMATLMPILEM
NNLMSR
>Mature_405_residues
AAFEYKALDAKGRHKKGVIEGDNARQVRQRLKEQSLVPMEVVETQVKAARSRSQGFAFKRGISTPDLALITRQLATLVQS
GMPLEECLRAVAEQSEKPRIRTMLVAVRAKVTEGYTLSDSLGDYPHVFDELFRSMVAAGEKSGHLDSVLERLADYAENRQ
KMRSKLQQAMIYPVVLVVFAVGIVAFLLAAVVPKIVGQFVQMGQALPASTQFLLDASDFLQHWGISLLVGLLMLIYLVRW
LLTKPDIRLRWDRRVISLPVIGKIARGLNTARFARTLSICTSSAIPILDGMRVAVDVMTNQFVKQQVLAAAENVREGSSL
RKALEQTKLFPPMMLHMIASGEQSGELEGMLTRAADNQDNSFESTVNIALGIFTPALIALMAGMVLFIVMATLMPILEMN
NLMSR

Specific function: Required for secretion of cholera toxin through the outer membrane

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family

Homologues:

Organism=Escherichia coli, GI1789724, Length=403, Percent_Identity=44.1687344913151, Blast_Score=344, Evalue=5e-96,
Organism=Escherichia coli, GI1786295, Length=409, Percent_Identity=24.2053789731051, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSPF_VIBCH (P45780)

Other databases:

- EMBL:   L33796
- EMBL:   AE003852
- PIR:   E82041
- RefSeq:   NP_232358.1
- PDB:   2VMA
- PDB:   2VMB
- PDB:   3C1Q
- PDBsum:   2VMA
- PDBsum:   2VMB
- PDBsum:   3C1Q
- ProteinModelPortal:   P45780
- GeneID:   2614894
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2731
- TIGR:   VC_2731
- HOGENOM:   HBG635225
- OMA:   GMLQRRR
- ProtClustDB:   CLSK874902
- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992
- PRINTS:   PR00812
- TIGRFAMs:   TIGR02120

Pfam domain/function: PF00482 GSPII_F

EC number: NA

Molecular weight: Translated: 44932; Mature: 44800

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b8ba8ec)-; HASH(0x1b7342ec)-; HASH(0x1b801100)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
AAFEYKALDAKGRHKKGVIEGDNARQVRQRLKEQSLVPMEVVETQVKAARSRSQGFAFK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHH
RGISTPDLALITRQLATLVQSGMPLEECLRAVAEQSEKPRIRTMLVAVRAKVTEGYTLSD
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEECC
SLGDYPHVFDELFRSMVAAGEKSGHLDSVLERLADYAENRQKMRSKLQQAMIYPVVLVVF
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGIVAFLLAAVVPKIVGQFVQMGQALPASTQFLLDASDFLQHWGISLLVGLLMLIYLVR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHCCHHHHHHHHHHHHHHH
WLLTKPDIRLRWDRRVISLPVIGKIARGLNTARFARTLSICTSSAIPILDGMRVAVDVMT
HHHCCCCCEEEECCCEEECHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
NQFVKQQVLAAAENVREGSSLRKALEQTKLFPPMMLHMIASGEQSGELEGMLTRAADNQD
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCC
NSFESTVNIALGIFTPALIALMAGMVLFIVMATLMPILEMNNLMSR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301