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The map label for this gene is 147674997

Identifier: 147674997

GI number: 147674997

Start: 1066329

End: 1067903

Strand: Reverse

Name: 147674997

Synonym: VC0395_A1024

Alternate gene names: NA

Gene position: 1067903-1066329 (Counterclockwise)

Preceding gene: 147673881

Following gene: 147675234

Centisome position: 35.31

GC content: 48.19

Gene sequence:

>1575_bases
TTGATGAAAAAACGCGCTTCTCATCACTTGGTTATCTGCACCTTGATTGATACACTTCGCTTTCGTTTATCGTCACGGAA
GGCTATTTCCATGAATGCGTTCAATAAATTAGTAAAACATTCTAAAAAAATTTCTAACTTTAATCATTTGTCGTCCATCG
TTGGTTGGGACCAAGCCGCCGTCATGCCCAGTGGCGGCGCTGAAGCGCGCTCGCAAGCCATGGCAGAGCTTTCTGTGCAT
ATCCATGGCCTGATGACCCAGCCACAATTGGCTGATTGGTTTGCCGAGGCAGAAAGTGAAACGCTCAATATCGAGCAGCA
AGCCACCCTGCGCGAAATGAAACGCCAATGGCAACAAGCGACGGTTCTGCCTGAAGCCTTAGTCGAAGCGCAATCCCTAG
CTGGCTCCAAATGTGAACACGCTTGGCGTAGCCAACGCAAAAATAACGATTGGACAGGTTTTGAGAAAAACTGGGCGGAA
GTGGTCAAGCTTTCTCAAGAAGAAGCGCAAATTCGTGCCGAAGCAACTGGGAAAACCCCTTACGATGCGATGCTTGAGCT
TTATGAGCCGGGCACGACAACCGAACAGCTCAATCGTGTATTTAGTGATGTGAAAACTTGGCTACCAAGCTTAATTGATG
TGGTGATTGAAAAGCAGAGTAGCGAAAGCTTTATCGCACCTAAAGGCCATTATCCTGCTGAAAGCCAAAAAGCGCTGGGG
CTGGATGTGATGAAGCTACTTCAGTTTGATTTTAACCACGGCCGACTTGATGAAAGTGTGCACCCCTTCTGTGGCGGCGT
ACCGAGTGATGTGCGCATTACTACACGCTACAACGAAAGCGAGTTTGTACAATCGCTGATGGGCATTGTGCATGAGACTG
GCCATGCGCGTTACGAGCAAGGTTTACCTAAGCATTTGGCTGGCACTCCGGCTGGCGAAGCGCGTTCGATGGGCATTCAT
GAATCACAATCTCTGTTCTTTGAGATGCAAGTGGGTCGTAGCCCTGCGTTTATTGCCCATCTCGCTGAACTGGCAGGCAA
ACATTTTTCGGCGATGAATGATCCGGTCTTCCGAGTGGAAAACATTCAAAAACTTTACACGCGCGTGCAGAAAGATTTTA
TCCGCGTCGATGCGGATGAACTCACCTACCCAGCCCATGTGATCCTGCGTTTTGAAATCGAACGAGATTTGATGAACGGC
AAGATCAAACACACTGATGTGCCAGAACTTTGGGATCAAAAGATGCAGGCTTATTTAGGCCTAAGCACCAAAGGCAACGA
TCAAAATGGGTGTATGCAGGATATTCACTGGACAGATGGCAGCTTTGGTTACTTCCCAAGCTACACCTTAGGTGCCATGT
ATGCTGCGCAATTTATGGCGGCCATGAAGAAAACCGTGGATGTGGATGGCGCGATTCGCCGCGGCGATCTCTCACCAATA
TTCTCTTGGTTGTCTGAGAATATTTGGAGCAAGGGCAGCTTATTCAGTACCGATGAATTGGTTAAACAAGCGACGGGGGA
AACCCTCAACCCAGAGCATTTTAAAGCTCATTTGAGTCAGCGCTATCTGAAGTAA

Upstream 100 bases:

>100_bases
CACACCATTCAAAATCAATAAGTTATAGAAATAGTCCCATCAACAAAGCAAAAAACTGCCCAACGCTGAGCAAGATCTTG
CTTGGCGGGCAAAAATCCTC

Downstream 100 bases:

>100_bases
TCTTTCACGAACCCCACGAAGTGTGGGGTTCTTTATTTCCTCAAAATCTCACGTCTTCACCGTCATTTTTCAGCGCATGC
TAGATAATATTTGTAAATGA

Product: thermostable carboxypeptidase 1

Products: NA

Alternate protein names: Carboxypeptidase Taq [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MMKKRASHHLVICTLIDTLRFRLSSRKAISMNAFNKLVKHSKKISNFNHLSSIVGWDQAAVMPSGGAEARSQAMAELSVH
IHGLMTQPQLADWFAEAESETLNIEQQATLREMKRQWQQATVLPEALVEAQSLAGSKCEHAWRSQRKNNDWTGFEKNWAE
VVKLSQEEAQIRAEATGKTPYDAMLELYEPGTTTEQLNRVFSDVKTWLPSLIDVVIEKQSSESFIAPKGHYPAESQKALG
LDVMKLLQFDFNHGRLDESVHPFCGGVPSDVRITTRYNESEFVQSLMGIVHETGHARYEQGLPKHLAGTPAGEARSMGIH
ESQSLFFEMQVGRSPAFIAHLAELAGKHFSAMNDPVFRVENIQKLYTRVQKDFIRVDADELTYPAHVILRFEIERDLMNG
KIKHTDVPELWDQKMQAYLGLSTKGNDQNGCMQDIHWTDGSFGYFPSYTLGAMYAAQFMAAMKKTVDVDGAIRRGDLSPI
FSWLSENIWSKGSLFSTDELVKQATGETLNPEHFKAHLSQRYLK

Sequences:

>Translated_524_residues
MMKKRASHHLVICTLIDTLRFRLSSRKAISMNAFNKLVKHSKKISNFNHLSSIVGWDQAAVMPSGGAEARSQAMAELSVH
IHGLMTQPQLADWFAEAESETLNIEQQATLREMKRQWQQATVLPEALVEAQSLAGSKCEHAWRSQRKNNDWTGFEKNWAE
VVKLSQEEAQIRAEATGKTPYDAMLELYEPGTTTEQLNRVFSDVKTWLPSLIDVVIEKQSSESFIAPKGHYPAESQKALG
LDVMKLLQFDFNHGRLDESVHPFCGGVPSDVRITTRYNESEFVQSLMGIVHETGHARYEQGLPKHLAGTPAGEARSMGIH
ESQSLFFEMQVGRSPAFIAHLAELAGKHFSAMNDPVFRVENIQKLYTRVQKDFIRVDADELTYPAHVILRFEIERDLMNG
KIKHTDVPELWDQKMQAYLGLSTKGNDQNGCMQDIHWTDGSFGYFPSYTLGAMYAAQFMAAMKKTVDVDGAIRRGDLSPI
FSWLSENIWSKGSLFSTDELVKQATGETLNPEHFKAHLSQRYLK
>Mature_524_residues
MMKKRASHHLVICTLIDTLRFRLSSRKAISMNAFNKLVKHSKKISNFNHLSSIVGWDQAAVMPSGGAEARSQAMAELSVH
IHGLMTQPQLADWFAEAESETLNIEQQATLREMKRQWQQATVLPEALVEAQSLAGSKCEHAWRSQRKNNDWTGFEKNWAE
VVKLSQEEAQIRAEATGKTPYDAMLELYEPGTTTEQLNRVFSDVKTWLPSLIDVVIEKQSSESFIAPKGHYPAESQKALG
LDVMKLLQFDFNHGRLDESVHPFCGGVPSDVRITTRYNESEFVQSLMGIVHETGHARYEQGLPKHLAGTPAGEARSMGIH
ESQSLFFEMQVGRSPAFIAHLAELAGKHFSAMNDPVFRVENIQKLYTRVQKDFIRVDADELTYPAHVILRFEIERDLMNG
KIKHTDVPELWDQKMQAYLGLSTKGNDQNGCMQDIHWTDGSFGYFPSYTLGAMYAAQFMAAMKKTVDVDGAIRRGDLSPI
FSWLSENIWSKGSLFSTDELVKQATGETLNPEHFKAHLSQRYLK

Specific function: Releases amino acids sequentially from the C-terminus with a broad substrate specificity (except for proline) [H]

COG id: COG2317

COG function: function code E; Zn-dependent carboxypeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M32 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001333 [H]

Pfam domain/function: PF02074 Peptidase_M32 [H]

EC number: =3.4.17.19 [H]

Molecular weight: Translated: 59196; Mature: 59196

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKRASHHLVICTLIDTLRFRLSSRKAISMNAFNKLVKHSKKISNFNHLSSIVGWDQAA
CCCCCCCCCEEHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCE
VMPSGGAEARSQAMAELSVHIHGLMTQPQLADWFAEAESETLNIEQQATLREMKRQWQQA
ECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TVLPEALVEAQSLAGSKCEHAWRSQRKNNDWTGFEKNWAEVVKLSQEEAQIRAEATGKTP
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
YDAMLELYEPGTTTEQLNRVFSDVKTWLPSLIDVVIEKQSSESFIAPKGHYPAESQKALG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
LDVMKLLQFDFNHGRLDESVHPFCGGVPSDVRITTRYNESEFVQSLMGIVHETGHARYEQ
HHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHC
GLPKHLAGTPAGEARSMGIHESQSLFFEMQVGRSPAFIAHLAELAGKHFSAMNDPVFRVE
CCCHHHCCCCCCCHHHCCCCCCHHEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
NIQKLYTRVQKDFIRVDADELTYPAHVILRFEIERDLMNGKIKHTDVPELWDQKMQAYLG
HHHHHHHHHHHHHHHCCHHHHCCCEEEEEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHC
LSTKGNDQNGCMQDIHWTDGSFGYFPSYTLGAMYAAQFMAAMKKTVDVDGAIRRGDLSPI
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
FSWLSENIWSKGSLFSTDELVKQATGETLNPEHFKAHLSQRYLK
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MMKKRASHHLVICTLIDTLRFRLSSRKAISMNAFNKLVKHSKKISNFNHLSSIVGWDQAA
CCCCCCCCCEEHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCE
VMPSGGAEARSQAMAELSVHIHGLMTQPQLADWFAEAESETLNIEQQATLREMKRQWQQA
ECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TVLPEALVEAQSLAGSKCEHAWRSQRKNNDWTGFEKNWAEVVKLSQEEAQIRAEATGKTP
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
YDAMLELYEPGTTTEQLNRVFSDVKTWLPSLIDVVIEKQSSESFIAPKGHYPAESQKALG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
LDVMKLLQFDFNHGRLDESVHPFCGGVPSDVRITTRYNESEFVQSLMGIVHETGHARYEQ
HHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHC
GLPKHLAGTPAGEARSMGIHESQSLFFEMQVGRSPAFIAHLAELAGKHFSAMNDPVFRVE
CCCHHHCCCCCCCHHHCCCCCCHHEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
NIQKLYTRVQKDFIRVDADELTYPAHVILRFEIERDLMNGKIKHTDVPELWDQKMQAYLG
HHHHHHHHHHHHHHHCCHHHHCCCEEEEEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHC
LSTKGNDQNGCMQDIHWTDGSFGYFPSYTLGAMYAAQFMAAMKKTVDVDGAIRRGDLSPI
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
FSWLSENIWSKGSLFSTDELVKQATGETLNPEHFKAHLSQRYLK
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765282; 1369078 [H]