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The map label for this gene is dnaG [H]

Identifier: 147674914

GI number: 147674914

Start: 43166

End: 44995

Strand: Reverse

Name: dnaG [H]

Synonym: VC0395_A0045

Alternate gene names: 147674914

Gene position: 44995-43166 (Counterclockwise)

Preceding gene: 147675525

Following gene: 147674012

Centisome position: 1.49

GC content: 46.28

Gene sequence:

>1830_bases
GTGCAATCCTTTGGAAAGCACGGCTTGTTTGTATTTGACCCTATCTTTCGGTGCATGCAAAGATTTATGGCAGGACACAT
CCCCCGCAGTTTTATTGATGACCTCCTTGCTCGACTCGATATTGTCGACGTTATTGACGCACGCGTAAAACTGAAGAAGA
AAGGCAAAAACTACAGCGCCTGCTGCCCTTTCCATAACGAAAAAACCCCTTCATTCAGTGTCAGTCAGGAAAAACAGTTC
TATCACTGCTTCGGCTGTGGTGCTCATGGCAATGCCATCGATTTTGTCATGGAATATGAACGCATGGAGTTTCCTGAGGC
CATTGAAGAGCTGGCGTCGACCATAGGTCTTGAAGTTCAGCGTGAAGAAAGAACGCCTTCCAGCCCCTTTGCGAAAAATA
CGCCAGCCGTCAAAAGTGAAGATAAGCGCAGCCTGTATGACTTGATGGGCAGCATCGCCCAGTTTTATCGTCAGCAGCTT
AAAGTTCCTGCCAACAAGCATGCGATTGAATACTTAAAAAATCGTGGTTTATCTGGAGAAATCGTCCAGAAATTTGGCAT
TGGATACATAGCGGATGAATGGGATCTTGTACGGCGCAATTTTGGTCAGCAGCGCAGCAGTGAAGATATGCTTGTCACCG
CAGGTATGCTGATTGAAAACGATAACGGCCGCCGTTATGACCGCTTTCGCGGACGAGTCATGTTCCCAATTCGCGATCGT
CGAGGTCGAGTCATTGGTTTTGGTGGCCGTATTACAGAGCAAGGTACACCGAAATACCTGAACTCGCCTGAAACCCCGAT
TTTCCATAAAGGCAAAGAGCTGTATGGCCTTTATGAAGTCCTGCAGGCATACCGTGAACCACCACAAATCCTAGTGGTGG
AAGGCTATATGGATGTCGTGGCACTCGCTCAATATGGTGTCGATTACTCTGTAGCCTCTCTGGGCACCTCAACCACAGGC
GATCACTTACAACTGTTGTTTCGCCAAACTAACCATGTCATCTGTTGTTATGATGGTGACCGAGCTGGACGTGATGCCGC
ATGGCGCGCATTAGAAAATGCACTCAGCTACCTCAAAAGCGGCAATATCTTAAAGTTTCTGTTTCTGCCGGACGGTGAAG
ATCCCGATAGCTACGTGCGTAAATACGGCAAAGCCGATTTTGAACAACAGGTTGCCAATGCTACACCACTGTCGCAGTAC
TTATTTGAGAACTTGATTGAACTGCACCAAATTAACTTGGGTAGCCATGAAGGTAAATCGGCACTGCGCGCCGTTGCAAC
ACCACTTATCGACAAAATTCCGGATCCTTTTTTACAAGAGATCCTAGAGAAATTACTCGATGAACGGACAGGCTTTGATC
ACCAACTGCGCCGTAAAAAAGCACGTAAGACAGAAAATCGACCCGCACCACATAAAGCAATAAAACGGACCCCAATGCGT
GATGTTATCGCGCTCTTGATACAAAATCCGAGCTATGCTGAATTGGTACCAGACCTAGCGAGCGTGCGTCATTTGATGAT
CCCTGGTCTCGATACCTTCAGTGAAGTACTTGAGAAATGTCGTCAATATCCCCATATCACTACGGGTCAATTACTCGAAC
ATTGGCGAGACTCAAAAAATGAGACACTTCTGTCTCGTCTGGCAAGTTGGGAAATTCCCCTTGTCGAAGACAATCAAGAA
GAACTATTTTTAGACTCATTGGACAAAATTCTTGCCCAGTGCGTAGAAAAACAAATTGAAAATTTGCAGGCCAAAGAGCG
AAGTGTCGGTTTATCAACCGAAGAGAAAAGAGAGCTACAAGACTTGATCTTAAAAGGCTTGAAAGCGTAA

Upstream 100 bases:

>100_bases
CTGTACTTAAACCGCATATTCAAGGGCGTGCAGACATGGGTAAAGTGAGCGGTTTGGTCCGCGCTAAACTGGCTTAATCC
CAACCCAATTACAGCAAGCC

Downstream 100 bases:

>100_bases
CTCTGTTGAGATAGTCAGCAAAGAATAAATTTGTTAGTATACGTGGTTTGCATTGCGCATACTTATTCCTTCACCAGATA
CGAAGTTGGATACCGTCTAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MQSFGKHGLFVFDPIFRCMQRFMAGHIPRSFIDDLLARLDIVDVIDARVKLKKKGKNYSACCPFHNEKTPSFSVSQEKQF
YHCFGCGAHGNAIDFVMEYERMEFPEAIEELASTIGLEVQREERTPSSPFAKNTPAVKSEDKRSLYDLMGSIAQFYRQQL
KVPANKHAIEYLKNRGLSGEIVQKFGIGYIADEWDLVRRNFGQQRSSEDMLVTAGMLIENDNGRRYDRFRGRVMFPIRDR
RGRVIGFGGRITEQGTPKYLNSPETPIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTG
DHLQLLFRQTNHVICCYDGDRAGRDAAWRALENALSYLKSGNILKFLFLPDGEDPDSYVRKYGKADFEQQVANATPLSQY
LFENLIELHQINLGSHEGKSALRAVATPLIDKIPDPFLQEILEKLLDERTGFDHQLRRKKARKTENRPAPHKAIKRTPMR
DVIALLIQNPSYAELVPDLASVRHLMIPGLDTFSEVLEKCRQYPHITTGQLLEHWRDSKNETLLSRLASWEIPLVEDNQE
ELFLDSLDKILAQCVEKQIENLQAKERSVGLSTEEKRELQDLILKGLKA

Sequences:

>Translated_609_residues
MQSFGKHGLFVFDPIFRCMQRFMAGHIPRSFIDDLLARLDIVDVIDARVKLKKKGKNYSACCPFHNEKTPSFSVSQEKQF
YHCFGCGAHGNAIDFVMEYERMEFPEAIEELASTIGLEVQREERTPSSPFAKNTPAVKSEDKRSLYDLMGSIAQFYRQQL
KVPANKHAIEYLKNRGLSGEIVQKFGIGYIADEWDLVRRNFGQQRSSEDMLVTAGMLIENDNGRRYDRFRGRVMFPIRDR
RGRVIGFGGRITEQGTPKYLNSPETPIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTG
DHLQLLFRQTNHVICCYDGDRAGRDAAWRALENALSYLKSGNILKFLFLPDGEDPDSYVRKYGKADFEQQVANATPLSQY
LFENLIELHQINLGSHEGKSALRAVATPLIDKIPDPFLQEILEKLLDERTGFDHQLRRKKARKTENRPAPHKAIKRTPMR
DVIALLIQNPSYAELVPDLASVRHLMIPGLDTFSEVLEKCRQYPHITTGQLLEHWRDSKNETLLSRLASWEIPLVEDNQE
ELFLDSLDKILAQCVEKQIENLQAKERSVGLSTEEKRELQDLILKGLKA
>Mature_609_residues
MQSFGKHGLFVFDPIFRCMQRFMAGHIPRSFIDDLLARLDIVDVIDARVKLKKKGKNYSACCPFHNEKTPSFSVSQEKQF
YHCFGCGAHGNAIDFVMEYERMEFPEAIEELASTIGLEVQREERTPSSPFAKNTPAVKSEDKRSLYDLMGSIAQFYRQQL
KVPANKHAIEYLKNRGLSGEIVQKFGIGYIADEWDLVRRNFGQQRSSEDMLVTAGMLIENDNGRRYDRFRGRVMFPIRDR
RGRVIGFGGRITEQGTPKYLNSPETPIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTG
DHLQLLFRQTNHVICCYDGDRAGRDAAWRALENALSYLKSGNILKFLFLPDGEDPDSYVRKYGKADFEQQVANATPLSQY
LFENLIELHQINLGSHEGKSALRAVATPLIDKIPDPFLQEILEKLLDERTGFDHQLRRKKARKTENRPAPHKAIKRTPMR
DVIALLIQNPSYAELVPDLASVRHLMIPGLDTFSEVLEKCRQYPHITTGQLLEHWRDSKNETLLSRLASWEIPLVEDNQE
ELFLDSLDKILAQCVEKQIENLQAKERSVGLSTEEKRELQDLILKGLKA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=587, Percent_Identity=54.5144804088586, Blast_Score=633, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 69613; Mature: 69613

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSFGKHGLFVFDPIFRCMQRFMAGHIPRSFIDDLLARLDIVDVIDARVKLKKKGKNYSA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
CCPFHNEKTPSFSVSQEKQFYHCFGCGAHGNAIDFVMEYERMEFPEAIEELASTIGLEVQ
ECCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCEEE
REERTPSSPFAKNTPAVKSEDKRSLYDLMGSIAQFYRQQLKVPANKHAIEYLKNRGLSGE
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHH
IVQKFGIGYIADEWDLVRRNFGQQRSSEDMLVTAGMLIENDNGRRYDRFRGRVMFPIRDR
HHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEECEEEECCCCCHHHHHCCEEEEEEECC
RGRVIGFGGRITEQGTPKYLNSPETPIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVV
CCCEEEECCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHH
ALAQYGVDYSVASLGTSTTGDHLQLLFRQTNHVICCYDGDRAGRDAAWRALENALSYLKS
HHHHHCCCHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GNILKFLFLPDGEDPDSYVRKYGKADFEQQVANATPLSQYLFENLIELHQINLGSHEGKS
CCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHH
ALRAVATPLIDKIPDPFLQEILEKLLDERTGFDHQLRRKKARKTENRPAPHKAIKRTPMR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHCCCHH
DVIALLIQNPSYAELVPDLASVRHLMIPGLDTFSEVLEKCRQYPHITTGQLLEHWRDSKN
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCH
ETLLSRLASWEIPLVEDNQEELFLDSLDKILAQCVEKQIENLQAKERSVGLSTEEKRELQ
HHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
DLILKGLKA
HHHHHHCCC
>Mature Secondary Structure
MQSFGKHGLFVFDPIFRCMQRFMAGHIPRSFIDDLLARLDIVDVIDARVKLKKKGKNYSA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
CCPFHNEKTPSFSVSQEKQFYHCFGCGAHGNAIDFVMEYERMEFPEAIEELASTIGLEVQ
ECCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCEEE
REERTPSSPFAKNTPAVKSEDKRSLYDLMGSIAQFYRQQLKVPANKHAIEYLKNRGLSGE
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHH
IVQKFGIGYIADEWDLVRRNFGQQRSSEDMLVTAGMLIENDNGRRYDRFRGRVMFPIRDR
HHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEECEEEECCCCCHHHHHCCEEEEEEECC
RGRVIGFGGRITEQGTPKYLNSPETPIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVV
CCCEEEECCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHH
ALAQYGVDYSVASLGTSTTGDHLQLLFRQTNHVICCYDGDRAGRDAAWRALENALSYLKS
HHHHHCCCHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GNILKFLFLPDGEDPDSYVRKYGKADFEQQVANATPLSQYLFENLIELHQINLGSHEGKS
CCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHH
ALRAVATPLIDKIPDPFLQEILEKLLDERTGFDHQLRRKKARKTENRPAPHKAIKRTPMR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHCCCHH
DVIALLIQNPSYAELVPDLASVRHLMIPGLDTFSEVLEKCRQYPHITTGQLLEHWRDSKN
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCH
ETLLSRLASWEIPLVEDNQEELFLDSLDKILAQCVEKQIENLQAKERSVGLSTEEKRELQ
HHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
DLILKGLKA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]