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The map label for this gene is proA [H]

Identifier: 147674854

GI number: 147674854

Start: 1980342

End: 1981595

Strand: Reverse

Name: proA [H]

Synonym: VC0395_A1863

Alternate gene names: 147674854

Gene position: 1981595-1980342 (Counterclockwise)

Preceding gene: 162329621

Following gene: 147674568

Centisome position: 65.53

GC content: 51.83

Gene sequence:

>1254_bases
ATGATGGATTTAACCGTACTCGGTAAAGCGGCCAAAGCGGCCTCTTTCCAACTGGCTACCGCCAGTACCGCGCAAAAAAA
TCAGGCTCTCGCCAGTATGGCCGATCAGTTGGAAGCGCAAAGCGCGAGCATTCTTGCTGCAAACGCAAAAGATATTGCGC
TGGGGCGCGAAGCGGGTTTGTCTGATGCCATGCTGGATCGCTTGCTGCTCAATGAGTCTCGCTTGCAAGCAATCGCCAAC
GATGTGCGTAACGTGATCAAATTGAACGATCCCGTCGGCAGCGAAATTGATAGCCGAGTGTTGGAAAACGGCATGTCATT
GGCACGCCGTCGCGTCCCCCTTGGTGTGGTCGGCGTCATTTATGAAGCTCGCCCTAACGTCACCATTGATATTGCGGCGC
TGTGTTTAAAAACCGGTAACGCCGCGATTTTGCGCGGTGGCAAAGAGACGTTTTTCTCTAACATGGAACTGGTCAAAGTG
ATCCAGTCGGCGTTGGATAAAGCAGGTCTGCCAGCGGCTTCCGTGCAGTACATTGAAAAGCCAGATCGTGAACTGGTGAC
GCAACTCCTGAAAATGGATGATTACGTCGACATGATCATCCCACGTGGCGGTGCGGGCCTGCACAAAATGTGCAAAGAAA
ACAGCACAGTGCCTGTGATCATTGGAGGCTTTGGCATTAGCCATATTTTCGTGGATGAAAGTGCTGATCTGGATAAATCG
GTTGCCGTAATTGAAAACGCTAAAGTGCAACGCCCATCGGCGTGTAATGCGCTAGATACCTTGTTAGTGCATCAAGCGAT
TGCCAAACCGTTACTAGACAAACTCATCGCAAAACTGAATGGCAAAGTGGCGTTTGTGGCGGAGCCCAAAGCCAAGGCGT
TGATGAGTTCAGCGGCTGAGCTGCGTGATGCGCAAGCGGGCGATTTTGATACCGAATGGCTCAGCTACACCTTAGGGGTG
AAAGTGGTGCAGGATGTACAAGAAGCCATTGAGCATATGCGCGAGCACAATGCGAGCCATTCCGATGCGATCATGACCAA
CGATCTCTATAACGCTGAGCTGTTTGTGAATACCGCCGGTTCAGCGGCGGTGTATGTCAACGCTTCTACGCGTTTTACTG
ACGGTGCACAGTTTGGTTTAGGCGCAGAAGTGGCAGTCTCAACACAAAAACTGCACGCGCGTGGTCCGATGGGGCTAGAA
GAGCTCACCAGCTACAAGTGGGTGGGCAAAGCTAATTACCTGTCACGCAGCTAA

Upstream 100 bases:

>100_bases
GTAAGCACAGTAAAGAGATCATCGATATTTTAGGCTACGATCACGGCTCAGAAGTGATCCACCGCGACGACATGGTCGTG
ATTCAAGAGTAAAGGAGAGA

Downstream 100 bases:

>100_bases
AATTCAAGCAAAGATGTGATGTAAAGGGGCTTCGCGCCCCTTTTTCTTTGGCTCTCAACAGCACATCGGCTACACTGGTT
GTCTTGTTATGGAGGTAATA

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MMDLTVLGKAAKAASFQLATASTAQKNQALASMADQLEAQSASILAANAKDIALGREAGLSDAMLDRLLLNESRLQAIAN
DVRNVIKLNDPVGSEIDSRVLENGMSLARRRVPLGVVGVIYEARPNVTIDIAALCLKTGNAAILRGGKETFFSNMELVKV
IQSALDKAGLPAASVQYIEKPDRELVTQLLKMDDYVDMIIPRGGAGLHKMCKENSTVPVIIGGFGISHIFVDESADLDKS
VAVIENAKVQRPSACNALDTLLVHQAIAKPLLDKLIAKLNGKVAFVAEPKAKALMSSAAELRDAQAGDFDTEWLSYTLGV
KVVQDVQEAIEHMREHNASHSDAIMTNDLYNAELFVNTAGSAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLE
ELTSYKWVGKANYLSRS

Sequences:

>Translated_417_residues
MMDLTVLGKAAKAASFQLATASTAQKNQALASMADQLEAQSASILAANAKDIALGREAGLSDAMLDRLLLNESRLQAIAN
DVRNVIKLNDPVGSEIDSRVLENGMSLARRRVPLGVVGVIYEARPNVTIDIAALCLKTGNAAILRGGKETFFSNMELVKV
IQSALDKAGLPAASVQYIEKPDRELVTQLLKMDDYVDMIIPRGGAGLHKMCKENSTVPVIIGGFGISHIFVDESADLDKS
VAVIENAKVQRPSACNALDTLLVHQAIAKPLLDKLIAKLNGKVAFVAEPKAKALMSSAAELRDAQAGDFDTEWLSYTLGV
KVVQDVQEAIEHMREHNASHSDAIMTNDLYNAELFVNTAGSAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLE
ELTSYKWVGKANYLSRS
>Mature_417_residues
MMDLTVLGKAAKAASFQLATASTAQKNQALASMADQLEAQSASILAANAKDIALGREAGLSDAMLDRLLLNESRLQAIAN
DVRNVIKLNDPVGSEIDSRVLENGMSLARRRVPLGVVGVIYEARPNVTIDIAALCLKTGNAAILRGGKETFFSNMELVKV
IQSALDKAGLPAASVQYIEKPDRELVTQLLKMDDYVDMIIPRGGAGLHKMCKENSTVPVIIGGFGISHIFVDESADLDKS
VAVIENAKVQRPSACNALDTLLVHQAIAKPLLDKLIAKLNGKVAFVAEPKAKALMSSAAELRDAQAGDFDTEWLSYTLGV
KVVQDVQEAIEHMREHNASHSDAIMTNDLYNAELFVNTAGSAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLE
ELTSYKWVGKANYLSRS

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=415, Percent_Identity=38.3132530120482, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI21361368, Length=415, Percent_Identity=38.3132530120482, Blast_Score=256, Evalue=2e-68,
Organism=Escherichia coli, GI1786438, Length=417, Percent_Identity=58.273381294964, Blast_Score=490, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17569967, Length=402, Percent_Identity=34.0796019900498, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17569969, Length=402, Percent_Identity=34.0796019900498, Blast_Score=230, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6324899, Length=419, Percent_Identity=37.2315035799523, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI21357643, Length=419, Percent_Identity=33.890214797136, Blast_Score=238, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 44636; Mature: 44636

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDLTVLGKAAKAASFQLATASTAQKNQALASMADQLEAQSASILAANAKDIALGREAGL
CCCHHHHCCHHHHCEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCCCCC
SDAMLDRLLLNESRLQAIANDVRNVIKLNDPVGSEIDSRVLENGMSLARRRVPLGVVGVI
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
YEARPNVTIDIAALCLKTGNAAILRGGKETFFSNMELVKVIQSALDKAGLPAASVQYIEK
EECCCCCEEEHHHHHHHCCCEEEEECCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCC
PDRELVTQLLKMDDYVDMIIPRGGAGLHKMCKENSTVPVIIGGFGISHIFVDESADLDKS
CCHHHHHHHHHHCCHHHHEECCCCCHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCHH
VAVIENAKVQRPSACNALDTLLVHQAIAKPLLDKLIAKLNGKVAFVAEPKAKALMSSAAE
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
LRDAQAGDFDTEWLSYTLGVKVVQDVQEAIEHMREHNASHSDAIMTNDLYNAELFVNTAG
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEECC
SAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEELTSYKWVGKANYLSRS
CEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MMDLTVLGKAAKAASFQLATASTAQKNQALASMADQLEAQSASILAANAKDIALGREAGL
CCCHHHHCCHHHHCEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCCCCC
SDAMLDRLLLNESRLQAIANDVRNVIKLNDPVGSEIDSRVLENGMSLARRRVPLGVVGVI
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
YEARPNVTIDIAALCLKTGNAAILRGGKETFFSNMELVKVIQSALDKAGLPAASVQYIEK
EECCCCCEEEHHHHHHHCCCEEEEECCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCC
PDRELVTQLLKMDDYVDMIIPRGGAGLHKMCKENSTVPVIIGGFGISHIFVDESADLDKS
CCHHHHHHHHHHCCHHHHEECCCCCHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCHH
VAVIENAKVQRPSACNALDTLLVHQAIAKPLLDKLIAKLNGKVAFVAEPKAKALMSSAAE
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
LRDAQAGDFDTEWLSYTLGVKVVQDVQEAIEHMREHNASHSDAIMTNDLYNAELFVNTAG
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEECC
SAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEELTSYKWVGKANYLSRS
CEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA