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The map label for this gene is gltT [H]

Identifier: 147674816

GI number: 147674816

Start: 765280

End: 766038

Strand: Reverse

Name: gltT [H]

Synonym: VC0395_A0737

Alternate gene names: 147674816

Gene position: 766038-765280 (Counterclockwise)

Preceding gene: 147675613

Following gene: 147673414

Centisome position: 25.33

GC content: 49.67

Gene sequence:

>759_bases
GTGCTCATTTTTATAGTCTCGATTGCTCATTTCTCGCGGCTCGCTACAACCATTGTTGATGCCATGGTGTGGATGATCAA
TAAAGTGATGCTGATTGCGCCGATTGGTGTATTTGGCTTAATGGCTGATGCCGTGGGTACTTTTGGTTTCAGCGCGCTGA
TGGTGGTGTTTAAGCTGTTCGTCGTTTACGTAGCGGCGATTGCGATTTTTGGCTTTGTGGCTTACCCAGTCATGATCCAA
CTGTTCAGCAAAACATCGGCGAAAAAGTTCATTCAAGCAATGAAAAAGCCACAAGCGGTTGCACTGTCTACGGCCTCTTC
TATGGCGACACTGCCTGTCACTATGGATACGGTGAAAAACGATCTGGGTGTGCGCAACTCAACGGCTTCTTTCGTACTGC
CTTTGGGCGCGACCATCAATATGTCAGGTAACGCGATCTACTACGGTCTGGTGGCTATCTTCTTTGCTCAGCTGTTTAAT
ATTGACCTGAGCATGGGCGCTTACATTGCGATCATCGTGACGGCGACTTTAGGTGCGGTTGGTCAAGCGGGTGTTCCGGG
GCCTTCATTCCTTGTGGTGGCGGTTCTGCTCTCTGCCGGTATTCCTATTGAAGGTCTACCACTGCTGTTTGCACTGGATC
GTATCTTTGACATGATCCGTACTGCGCTCAACATTACCGGCGATGCGGCTTGTGCAGTGATTGTGGATTCGATGATCACT
GAGGAAGCGGCGGCTGAGCTGGAAAAACGTGAAGCTTAA

Upstream 100 bases:

>100_bases
ACTACCGACGAACAAGGTACACCATCAATTCGCGGTGAATCACGCCCTGTAAATCCGATTTAAATTTTGTTTAACCCTAA
TGAAATGAGCACTATGGTTA

Downstream 100 bases:

>100_bases
TCGCTGATCATTATCATTCATAAAAAAAGCCAGACATTAACTGTCTGGCTTTTTCTTTATTGAGTCACGGGCAAATTATC
CCGCGAAGGAGATGGGTTCG

Product: proton/glutamate symporter

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLIFIVSIAHFSRLATTIVDAMVWMINKVMLIAPIGVFGLMADAVGTFGFSALMVVFKLFVVYVAAIAIFGFVAYPVMIQ
LFSKTSAKKFIQAMKKPQAVALSTASSMATLPVTMDTVKNDLGVRNSTASFVLPLGATINMSGNAIYYGLVAIFFAQLFN
IDLSMGAYIAIIVTATLGAVGQAGVPGPSFLVVAVLLSAGIPIEGLPLLFALDRIFDMIRTALNITGDAACAVIVDSMIT
EEAAAELEKREA

Sequences:

>Translated_252_residues
MLIFIVSIAHFSRLATTIVDAMVWMINKVMLIAPIGVFGLMADAVGTFGFSALMVVFKLFVVYVAAIAIFGFVAYPVMIQ
LFSKTSAKKFIQAMKKPQAVALSTASSMATLPVTMDTVKNDLGVRNSTASFVLPLGATINMSGNAIYYGLVAIFFAQLFN
IDLSMGAYIAIIVTATLGAVGQAGVPGPSFLVVAVLLSAGIPIEGLPLLFALDRIFDMIRTALNITGDAACAVIVDSMIT
EEAAAELEKREA
>Mature_252_residues
MLIFIVSIAHFSRLATTIVDAMVWMINKVMLIAPIGVFGLMADAVGTFGFSALMVVFKLFVVYVAAIAIFGFVAYPVMIQ
LFSKTSAKKFIQAMKKPQAVALSTASSMATLPVTMDTVKNDLGVRNSTASFVLPLGATINMSGNAIYYGLVAIFFAQLFN
IDLSMGAYIAIIVTATLGAVGQAGVPGPSFLVVAVLLSAGIPIEGLPLLFALDRIFDMIRTALNITGDAACAVIVDSMIT
EEAAAELEKREA

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI21314632, Length=250, Percent_Identity=32, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI4827012, Length=244, Percent_Identity=33.1967213114754, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI169790839, Length=239, Percent_Identity=33.4728033472803, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI223468566, Length=241, Percent_Identity=32.3651452282158, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI223468564, Length=241, Percent_Identity=32.3651452282158, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI5032093, Length=241, Percent_Identity=32.3651452282158, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI66773030, Length=235, Percent_Identity=34.0425531914894, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI194239697, Length=187, Percent_Identity=35.8288770053476, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI301601644, Length=151, Percent_Identity=39.7350993377483, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI40254478, Length=143, Percent_Identity=41.958041958042, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI262359914, Length=161, Percent_Identity=32.2981366459627, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1790514, Length=220, Percent_Identity=39.5454545454545, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789947, Length=231, Percent_Identity=30.3030303030303, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789473, Length=239, Percent_Identity=29.2887029288703, Blast_Score=80, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI193206654, Length=223, Percent_Identity=36.322869955157, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71983106, Length=214, Percent_Identity=35.0467289719626, Blast_Score=127, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71983099, Length=210, Percent_Identity=35.7142857142857, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI193206505, Length=229, Percent_Identity=32.3144104803493, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17537407, Length=235, Percent_Identity=31.9148936170213, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71996953, Length=221, Percent_Identity=30.7692307692308, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17541374, Length=238, Percent_Identity=34.453781512605, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24583025, Length=241, Percent_Identity=29.8755186721992, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17137668, Length=241, Percent_Identity=29.8755186721992, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24583023, Length=241, Percent_Identity=29.8755186721992, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI281360481, Length=233, Percent_Identity=30.9012875536481, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281360483, Length=233, Percent_Identity=30.9012875536481, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137666, Length=233, Percent_Identity=30.9012875536481, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 26633; Mature: 26633

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIFIVSIAHFSRLATTIVDAMVWMINKVMLIAPIGVFGLMADAVGTFGFSALMVVFKLF
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVYVAAIAIFGFVAYPVMIQLFSKTSAKKFIQAMKKPQAVALSTASSMATLPVTMDTVKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHCCCCHHHHHHH
DLGVRNSTASFVLPLGATINMSGNAIYYGLVAIFFAQLFNIDLSMGAYIAIIVTATLGAV
HCCCCCCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
GQAGVPGPSFLVVAVLLSAGIPIEGLPLLFALDRIFDMIRTALNITGDAACAVIVDSMIT
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
EEAAAELEKREA
HHHHHHHHHCCC
>Mature Secondary Structure
MLIFIVSIAHFSRLATTIVDAMVWMINKVMLIAPIGVFGLMADAVGTFGFSALMVVFKLF
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVYVAAIAIFGFVAYPVMIQLFSKTSAKKFIQAMKKPQAVALSTASSMATLPVTMDTVKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHCCCCHHHHHHH
DLGVRNSTASFVLPLGATINMSGNAIYYGLVAIFFAQLFNIDLSMGAYIAIIVTATLGAV
HCCCCCCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
GQAGVPGPSFLVVAVLLSAGIPIEGLPLLFALDRIFDMIRTALNITGDAACAVIVDSMIT
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
EEAAAELEKREA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1359385 [H]