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The map label for this gene is cfa [H]

Identifier: 147674745

GI number: 147674745

Start: 681151

End: 682449

Strand: Direct

Name: cfa [H]

Synonym: VC0395_A0640

Alternate gene names: 147674745

Gene position: 681151-682449 (Clockwise)

Preceding gene: 147673715

Following gene: 147673786

Centisome position: 22.52

GC content: 47.27

Gene sequence:

>1299_bases
GTGCCCCGTTTTATGCGCACCCAGACCAAACTCAGAACAATAATAAGGAGCAACCTCATGTTAAATAGCTCGTCGATGGA
AATATCCCGCTCTCTCACCAGTTGGCAGAAAGGTGCCCGCAGTATTATTCACCGTGCATTACAGTATCTCGAAGGCAATT
TAACAGTGATAGAACATTTCCCCGATCAAACCCAAACCACGCATGAACAATTTGGCCAAGATAAGCCCGATGCCATTCAT
GCCGTGATTGAAATTAAACACCCGGATTTTTACAGCCGAGTATTGAAGGGCGGGAGTATTGCTGCCGCAGAAGCTTACAT
GGAAGGGTGGTGGGAAAGCCCTAATCTGACGGCCTTGATGCAGTTGATGGCTGCCAACCTCGGTACGCTGGATAAGCTAG
AAAGTCAAAGTAGTCCTGTGACTCAATGGTTAAACCGTTTCACTCACTGGCTCAAACGCAACACCATAGATCAAGCCAAA
GATAATATTCACCAGCACTATGATTTGGGGAATGAGCTTTATCAACTGTTCCTCGATGAAGAGATGCTTTACTCCAGCGC
GCTGTTTACCCAACCTGAGCTCTCACTAGAGCAGGCGCAGCAAGCCAAAATGCAGCGTTTGTGTGAGCAACTACAACTGA
AACCGACCGATCACGTGTTAGAAATTGGTACGGGGTGGGGCGCGATGGCGATTTACATGGCGCAGCACTATGGCTGTAAG
GTCACCACCACCACCATTTCGGAAGAGCAGTATGCTTACGCACAGCAAAAAATTACGGCACTTGGGCTAAATAACCAAAT
TACCCTGCTCAAACAAGATTACCGTTTGCTGAGCGGTCAGTATGACAAGTTGGTATCGATTGAAATGATTGAAGCGGTGG
GTAAGGCCTACTTGCCGACGTTTTTGAGCCAATGTTATGCGTTACTGAAGCCGCGCGGCAAAATGGCGATTCAGGCAATC
ACCATTGCCGATCAGCGTTATGAAAGCTACAGCAATAACGTGGACTTTATTCAGAAATACATTTTCCCCGGCGGTTTTTT
GCCTTCGATCAGTGTGTTGACCGAATTAGCCACCAAACGAACTGGGTTGGTGTTGCGTAACTTGCATGACATCGGCATAG
ATTACGCACTGACGTTGCAACACTGGCGTGACCGATTTGAGCAGCAATTGCCGAAAGTACGTGATCTTGGGTATGACGAA
CGTTTCATCCGTATGTGGCGTTATTACTTTTGTTACTGCGAAGGTGGCTTTTTAGCCCGTAGCATCAGTACCGTGCACAT
GACTTTTGAACGAGATTAA

Upstream 100 bases:

>100_bases
AGCGCAAGCAATTTATTACCTCGATTGATCAAAACGCCGATCATGGCAGTAAAAGTGGTATTGGGCATTTATTGGCACGC
TTTCAAATTGTGGCGCAAAG

Downstream 100 bases:

>100_bases
TCATGGCGTTAGTGGCGATATCATTATGGTTTGACCTGCTCTGGGCACTGGCTGTCTTGGGGCAGCAAACCTGGTTATGG
CTCACCACATTACTGGTGGT

Product: cyclopropane-fatty-acyl-phospholipid synthase

Products: NA

Alternate protein names: CFA synthase; Cyclopropane fatty acid synthase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPRFMRTQTKLRTIIRSNLMLNSSSMEISRSLTSWQKGARSIIHRALQYLEGNLTVIEHFPDQTQTTHEQFGQDKPDAIH
AVIEIKHPDFYSRVLKGGSIAAAEAYMEGWWESPNLTALMQLMAANLGTLDKLESQSSPVTQWLNRFTHWLKRNTIDQAK
DNIHQHYDLGNELYQLFLDEEMLYSSALFTQPELSLEQAQQAKMQRLCEQLQLKPTDHVLEIGTGWGAMAIYMAQHYGCK
VTTTTISEEQYAYAQQKITALGLNNQITLLKQDYRLLSGQYDKLVSIEMIEAVGKAYLPTFLSQCYALLKPRGKMAIQAI
TIADQRYESYSNNVDFIQKYIFPGGFLPSISVLTELATKRTGLVLRNLHDIGIDYALTLQHWRDRFEQQLPKVRDLGYDE
RFIRMWRYYFCYCEGGFLARSISTVHMTFERD

Sequences:

>Translated_432_residues
MPRFMRTQTKLRTIIRSNLMLNSSSMEISRSLTSWQKGARSIIHRALQYLEGNLTVIEHFPDQTQTTHEQFGQDKPDAIH
AVIEIKHPDFYSRVLKGGSIAAAEAYMEGWWESPNLTALMQLMAANLGTLDKLESQSSPVTQWLNRFTHWLKRNTIDQAK
DNIHQHYDLGNELYQLFLDEEMLYSSALFTQPELSLEQAQQAKMQRLCEQLQLKPTDHVLEIGTGWGAMAIYMAQHYGCK
VTTTTISEEQYAYAQQKITALGLNNQITLLKQDYRLLSGQYDKLVSIEMIEAVGKAYLPTFLSQCYALLKPRGKMAIQAI
TIADQRYESYSNNVDFIQKYIFPGGFLPSISVLTELATKRTGLVLRNLHDIGIDYALTLQHWRDRFEQQLPKVRDLGYDE
RFIRMWRYYFCYCEGGFLARSISTVHMTFERD
>Mature_431_residues
PRFMRTQTKLRTIIRSNLMLNSSSMEISRSLTSWQKGARSIIHRALQYLEGNLTVIEHFPDQTQTTHEQFGQDKPDAIHA
VIEIKHPDFYSRVLKGGSIAAAEAYMEGWWESPNLTALMQLMAANLGTLDKLESQSSPVTQWLNRFTHWLKRNTIDQAKD
NIHQHYDLGNELYQLFLDEEMLYSSALFTQPELSLEQAQQAKMQRLCEQLQLKPTDHVLEIGTGWGAMAIYMAQHYGCKV
TTTTISEEQYAYAQQKITALGLNNQITLLKQDYRLLSGQYDKLVSIEMIEAVGKAYLPTFLSQCYALLKPRGKMAIQAIT
IADQRYESYSNNVDFIQKYIFPGGFLPSISVLTELATKRTGLVLRNLHDIGIDYALTLQHWRDRFEQQLPKVRDLGYDER
FIRMWRYYFCYCEGGFLARSISTVHMTFERD

Specific function: Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [H]

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family [H]

Homologues:

Organism=Escherichia coli, GI1787951, Length=359, Percent_Identity=37.6044568245125, Blast_Score=222, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI133904226, Length=392, Percent_Identity=38.7755102040816, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003333 [H]

Pfam domain/function: PF02353 CMAS [H]

EC number: =2.1.1.79 [H]

Molecular weight: Translated: 49964; Mature: 49833

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFMRTQTKLRTIIRSNLMLNSSSMEISRSLTSWQKGARSIIHRALQYLEGNLTVIEHF
CCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
PDQTQTTHEQFGQDKPDAIHAVIEIKHPDFYSRVLKGGSIAAAEAYMEGWWESPNLTALM
CCCCHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHH
QLMAANLGTLDKLESQSSPVTQWLNRFTHWLKRNTIDQAKDNIHQHYDLGNELYQLFLDE
HHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHH
EMLYSSALFTQPELSLEQAQQAKMQRLCEQLQLKPTDHVLEIGTGWGAMAIYMAQHYGCK
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCE
VTTTTISEEQYAYAQQKITALGLNNQITLLKQDYRLLSGQYDKLVSIEMIEAVGKAYLPT
EEEEECCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FLSQCYALLKPRGKMAIQAITIADQRYESYSNNVDFIQKYIFPGGFLPSISVLTELATKR
HHHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
TGLVLRNLHDIGIDYALTLQHWRDRFEQQLPKVRDLGYDERFIRMWRYYFCYCEGGFLAR
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHEEECCCHHHH
SISTVHMTFERD
HHEEEEEEEECC
>Mature Secondary Structure 
PRFMRTQTKLRTIIRSNLMLNSSSMEISRSLTSWQKGARSIIHRALQYLEGNLTVIEHF
CCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
PDQTQTTHEQFGQDKPDAIHAVIEIKHPDFYSRVLKGGSIAAAEAYMEGWWESPNLTALM
CCCCHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHH
QLMAANLGTLDKLESQSSPVTQWLNRFTHWLKRNTIDQAKDNIHQHYDLGNELYQLFLDE
HHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHH
EMLYSSALFTQPELSLEQAQQAKMQRLCEQLQLKPTDHVLEIGTGWGAMAIYMAQHYGCK
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCE
VTTTTISEEQYAYAQQKITALGLNNQITLLKQDYRLLSGQYDKLVSIEMIEAVGKAYLPT
EEEEECCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FLSQCYALLKPRGKMAIQAITIADQRYESYSNNVDFIQKYIFPGGFLPSISVLTELATKR
HHHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
TGLVLRNLHDIGIDYALTLQHWRDRFEQQLPKVRDLGYDERFIRMWRYYFCYCEGGFLAR
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHEEECCCHHHH
SISTVHMTFERD
HHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]