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The map label for this gene is mrcB

Identifier: 147674464

GI number: 147674464

Start: 119547

End: 121880

Strand: Direct

Name: mrcB

Synonym: VC0395_A0131

Alternate gene names: 147674464

Gene position: 119547-121880 (Clockwise)

Preceding gene: 147673788

Following gene: 147675227

Centisome position: 3.95

GC content: 51.54

Gene sequence:

>2334_bases
ATGACGAGAAAAAGTAGTAACAGATCGCGTGGCCGTAAAGCGCGCAGTGGTAAAAGTGCCTCTTCCTCCAAGCTGCAAAT
CTGGCTGGGAAGGATTTGGAGTATCGGCTGGAAATTAGCGCTGACGCTGGCCGCAGTGCTGGTTTTTATTGGTATCTATC
TCGATTCGATGATCAAACAGCGCTTTGAAGGTCAGCTGTTTGATCTGCCGACCGTGGTCTATGCCCGTATTTTAACGCTT
GAGCCCGGCAACGGCCTCAGTTTGCAAGAACTGCGCAATGAGCTGGATGTCCTCAATTACCGCAAAGTGGCACAGCCCCG
TTTCCCCGGTGAATACGCGTCTTCTTCCAGTCGAATTGAGCTGATCCGCCGTCCGTTTGAATTCGCGGATGGTCCAGAGC
CGGATCGCCGAGTGATGCTTACCTTTGATGGCAGTGGTTTGAACAAAATCGAATCATTAGAGCAAAAACGCGAGCTAGGC
TACCTGCGTTTAGAGCCAAAACTGATGGGCATGCTGGAAAAAGATTCGCCGGAGCAGCGACTCTTTTTACGTCGCGATCA
ATTCCCCGAAGTGTTGGTTGATGCACTCTTAGTCACCGAAGATCGTGATTTTTATCAGCACGATGGCGTATCACCACTTG
CGATTGGTCGCGCTATGGTGGTCAACCTTAAAGCGGGGCGCACGGTGCAAGGGGGCAGTACCTTGACCCAGCAGCTGGCT
AAAAACATCTTCCTTTCTAGCGATCGCACCTTGTGGCGTAAGCTGCGCGAAGCCTATATGGCGCTGATCATCGATTATCG
TTACAGCAAAGATCGTATTCTTGAAGCCTATCTTAACGAAGTTTATTTAGGGCAAAGTGGCGCAGATGCGATCCACGGTT
TTGGTCTGGCTTCACGGCTCTATTTTGGTCAGCCGCTGCAAGAGCTACGGATTGATCAATTGGCATTGTTGGTTGGCATG
GTTAAAGGGCCTTCTTATTACAACCCGATGCGTTATGCCGAGCGCGCTCGCGAGCGGCGCGATCTGGTGCTGAAACTGAT
GATGGAGCACGATATTCTGACGGCGCCAGAGTACCAGCAAGCGGTAACTCGTCCGTTGGATGTGCAGAAAACCGCGCAAA
TCGCTAGCCGTCAGCCAGCGTATTTCCAGCAAGTTTCTATTGAGCTGAAAGAAAAACTGGGCGACAAATTTAAAGCGGAT
TCAGGATTGCGGGTGTTTACCTCGCTCGATCCGGTTTCGCAGTCCAAACTCGAGCAAGCGATCCACGATCAGATCCCGCA
ATTAGCGAAAACCGCAGGCAAAGATCTCGAAGCCGCTGCGATCGCTGTGGATCGTCATAGTGGCGAAATTCGTGCCATGG
TCGGCGGTAAGCGAACCGGTTACGATGGTTTTAACCGCGTGCTCAATGCCAGTCGACAAATTGGTTCACTGGTGAAACCA
GCGGTGTATCTCACGGCGTTAGCGCATCCCGATCAGTACAACCTAGCCACTACACTGGAAGATAAACCACTCACCTTAAA
AGGCAGTGAAGGCAGCGCGTGGACTCCGCGAAACTACGATCGTCAATATCGTGGAGAGGTGCCCCTTTATTTGGCGCTCG
CTCAATCGCTTAATGTGCCGACCGTGGCTTTGGGGATGAAGCTTGGGATTGATCAAGTCTCAGCAACCTTGGGTAAGCTC
GGCGTGAATCGCGACGAAATTCGCCCTGTCCCTTCGATGTTGCTCGGTTCATTCTCACTCACGCCATATCAAGTGGCGCA
GATGTATCAAACGTTGACCAATTCCGGCAAAAAAGCCCCGCTGTCAGCGCTACGTTCTGTGCTCGATTTGGAAGGCAATG
TGCTGTATGAATCTCTGCCGAGAGTGTCACAAGCGGTGGATCAGCAAGCCGCTTGGCTGACGACTTACGCGATGAAACAA
GGGGTGCAAGAGGGGACTGGACGTTACTTGAATGCGCAGTTCAGCAGTGCAGCACTGGCGGGGAAAACCGGGACCACTAA
CGATAACCGTGACAGTTGGTTTGTGGGGGTCGATGGCCGTGAAGTGACCACCATTTGGCTTGGCCGCGATGATAACCAGC
CAACGAAGCTCACTGGCGCGAGTGGGGCATTACGTGTTTATGCGCAGTATTTGAAATATCGCATTCCAGAGAAATTACAG
TTACCTTGGCCAGAAGGCATAACGACCTTCGGCTTTGCCAAGCAGACGCAAGGTGGATTGAAACTCGACTGTGATAATGC
GTTTAAACTGCCTATTTGGGACAATCAACAGCAGTTAAAACAGCAGTGTGAAAACCGTCCGACCGAGTGGATCAAAAAGC
TGTTTGAGTGGTAA

Upstream 100 bases:

>100_bases
AAGTGCAAAAAGAGATGAAAGGCCGCTACCCGAAACACGTGTGGCCGGATGATCCTGCCAATCATGTGGCAACCAGTAAA
ACCAAACGACAATTGAACGC

Downstream 100 bases:

>100_bases
CACCCTCTTGGGCGCGGCGTTGTCGTTGCATTGACGACAACGTCTGCCTTGCTTGTGTGGGCATAGTTTTCTCTTTTCTT
GCTACTTGCTGCGGCAGCCG

Product: penicillin-binding protein 1B

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase

Number of amino acids: Translated: 777; Mature: 776

Protein sequence:

>777_residues
MTRKSSNRSRGRKARSGKSASSSKLQIWLGRIWSIGWKLALTLAAVLVFIGIYLDSMIKQRFEGQLFDLPTVVYARILTL
EPGNGLSLQELRNELDVLNYRKVAQPRFPGEYASSSSRIELIRRPFEFADGPEPDRRVMLTFDGSGLNKIESLEQKRELG
YLRLEPKLMGMLEKDSPEQRLFLRRDQFPEVLVDALLVTEDRDFYQHDGVSPLAIGRAMVVNLKAGRTVQGGSTLTQQLA
KNIFLSSDRTLWRKLREAYMALIIDYRYSKDRILEAYLNEVYLGQSGADAIHGFGLASRLYFGQPLQELRIDQLALLVGM
VKGPSYYNPMRYAERARERRDLVLKLMMEHDILTAPEYQQAVTRPLDVQKTAQIASRQPAYFQQVSIELKEKLGDKFKAD
SGLRVFTSLDPVSQSKLEQAIHDQIPQLAKTAGKDLEAAAIAVDRHSGEIRAMVGGKRTGYDGFNRVLNASRQIGSLVKP
AVYLTALAHPDQYNLATTLEDKPLTLKGSEGSAWTPRNYDRQYRGEVPLYLALAQSLNVPTVALGMKLGIDQVSATLGKL
GVNRDEIRPVPSMLLGSFSLTPYQVAQMYQTLTNSGKKAPLSALRSVLDLEGNVLYESLPRVSQAVDQQAAWLTTYAMKQ
GVQEGTGRYLNAQFSSAALAGKTGTTNDNRDSWFVGVDGREVTTIWLGRDDNQPTKLTGASGALRVYAQYLKYRIPEKLQ
LPWPEGITTFGFAKQTQGGLKLDCDNAFKLPIWDNQQQLKQQCENRPTEWIKKLFEW

Sequences:

>Translated_777_residues
MTRKSSNRSRGRKARSGKSASSSKLQIWLGRIWSIGWKLALTLAAVLVFIGIYLDSMIKQRFEGQLFDLPTVVYARILTL
EPGNGLSLQELRNELDVLNYRKVAQPRFPGEYASSSSRIELIRRPFEFADGPEPDRRVMLTFDGSGLNKIESLEQKRELG
YLRLEPKLMGMLEKDSPEQRLFLRRDQFPEVLVDALLVTEDRDFYQHDGVSPLAIGRAMVVNLKAGRTVQGGSTLTQQLA
KNIFLSSDRTLWRKLREAYMALIIDYRYSKDRILEAYLNEVYLGQSGADAIHGFGLASRLYFGQPLQELRIDQLALLVGM
VKGPSYYNPMRYAERARERRDLVLKLMMEHDILTAPEYQQAVTRPLDVQKTAQIASRQPAYFQQVSIELKEKLGDKFKAD
SGLRVFTSLDPVSQSKLEQAIHDQIPQLAKTAGKDLEAAAIAVDRHSGEIRAMVGGKRTGYDGFNRVLNASRQIGSLVKP
AVYLTALAHPDQYNLATTLEDKPLTLKGSEGSAWTPRNYDRQYRGEVPLYLALAQSLNVPTVALGMKLGIDQVSATLGKL
GVNRDEIRPVPSMLLGSFSLTPYQVAQMYQTLTNSGKKAPLSALRSVLDLEGNVLYESLPRVSQAVDQQAAWLTTYAMKQ
GVQEGTGRYLNAQFSSAALAGKTGTTNDNRDSWFVGVDGREVTTIWLGRDDNQPTKLTGASGALRVYAQYLKYRIPEKLQ
LPWPEGITTFGFAKQTQGGLKLDCDNAFKLPIWDNQQQLKQQCENRPTEWIKKLFEW
>Mature_776_residues
TRKSSNRSRGRKARSGKSASSSKLQIWLGRIWSIGWKLALTLAAVLVFIGIYLDSMIKQRFEGQLFDLPTVVYARILTLE
PGNGLSLQELRNELDVLNYRKVAQPRFPGEYASSSSRIELIRRPFEFADGPEPDRRVMLTFDGSGLNKIESLEQKRELGY
LRLEPKLMGMLEKDSPEQRLFLRRDQFPEVLVDALLVTEDRDFYQHDGVSPLAIGRAMVVNLKAGRTVQGGSTLTQQLAK
NIFLSSDRTLWRKLREAYMALIIDYRYSKDRILEAYLNEVYLGQSGADAIHGFGLASRLYFGQPLQELRIDQLALLVGMV
KGPSYYNPMRYAERARERRDLVLKLMMEHDILTAPEYQQAVTRPLDVQKTAQIASRQPAYFQQVSIELKEKLGDKFKADS
GLRVFTSLDPVSQSKLEQAIHDQIPQLAKTAGKDLEAAAIAVDRHSGEIRAMVGGKRTGYDGFNRVLNASRQIGSLVKPA
VYLTALAHPDQYNLATTLEDKPLTLKGSEGSAWTPRNYDRQYRGEVPLYLALAQSLNVPTVALGMKLGIDQVSATLGKLG
VNRDEIRPVPSMLLGSFSLTPYQVAQMYQTLTNSGKKAPLSALRSVLDLEGNVLYESLPRVSQAVDQQAAWLTTYAMKQG
VQEGTGRYLNAQFSSAALAGKTGTTNDNRDSWFVGVDGREVTTIWLGRDDNQPTKLTGASGALRVYAQYLKYRIPEKLQL
PWPEGITTFGFAKQTQGGLKLDCDNAFKLPIWDNQQQLKQQCENRPTEWIKKLFEW

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786343, Length=735, Percent_Identity=51.0204081632653, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI87082258, Length=249, Percent_Identity=36.5461847389558, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1788867, Length=513, Percent_Identity=29.0448343079922, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789601, Length=164, Percent_Identity=31.7073170731707, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPB_VIBCH (Q9KUC0)

Other databases:

- EMBL:   AE003852
- PIR:   A82303
- RefSeq:   NP_230252.1
- ProteinModelPortal:   Q9KUC0
- SMR:   Q9KUC0
- GeneID:   2615390
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0602
- TIGR:   VC_0602
- HOGENOM:   HBG672432
- OMA:   TRQPAYF
- ProtClustDB:   CLSK874029
- BRENDA:   2.4.1.129
- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460
- PIRSF:   PIRSF002799
- TIGRFAMs:   TIGR02071

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase; SSF56601 PBP_transp_fold

EC number: =2.4.1.129

Molecular weight: Translated: 87325; Mature: 87194

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: ACT_SITE 200-200 ACT_SITE 476-476

Signals:

None

Transmembrane regions:

HASH(0x17badf90)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKSSNRSRGRKARSGKSASSSKLQIWLGRIWSIGWKLALTLAAVLVFIGIYLDSMIKQ
CCCCCCCHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFEGQLFDLPTVVYARILTLEPGNGLSLQELRNELDVLNYRKVAQPRFPGEYASSSSRIE
HHCCCCCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHH
LIRRPFEFADGPEPDRRVMLTFDGSGLNKIESLEQKRELGYLRLEPKLMGMLEKDSPEQR
HHHCCHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECHHHHHHHCCCCCHHH
LFLRRDQFPEVLVDALLVTEDRDFYQHDGVSPLAIGRAMVVNLKAGRTVQGGSTLTQQLA
HHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCHHHHCCEEEEEECCCCEECCCHHHHHHHH
KNIFLSSDRTLWRKLREAYMALIIDYRYSKDRILEAYLNEVYLGQSGADAIHGFGLASRL
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
YFGQPLQELRIDQLALLVGMVKGPSYYNPMRYAERARERRDLVLKLMMEHDILTAPEYQQ
HCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
AVTRPLDVQKTAQIASRQPAYFQQVSIELKEKLGDKFKADSGLRVFTSLDPVSQSKLEQA
HHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCCCHHHHHHH
IHDQIPQLAKTAGKDLEAAAIAVDRHSGEIRAMVGGKRTGYDGFNRVLNASRQIGSLVKP
HHHHHHHHHHHCCCCCCHHEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
AVYLTALAHPDQYNLATTLEDKPLTLKGSEGSAWTPRNYDRQYRGEVPLYLALAQSLNVP
HHHHHHHCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCHHHCCCCCEEHHHHHHCCCC
TVALGMKLGIDQVSATLGKLGVNRDEIRPVPSMLLGSFSLTPYQVAQMYQTLTNSGKKAP
EEEHHHHHCHHHHHHHHHHCCCCHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCH
LSALRSVLDLEGNVLYESLPRVSQAVDQQAAWLTTYAMKQGVQEGTGRYLNAQFSSAALA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHCC
GKTGTTNDNRDSWFVGVDGREVTTIWLGRDDNQPTKLTGASGALRVYAQYLKYRIPEKLQ
CCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCEECCCCHHHHHHHHHHHHCCCCCCC
LPWPEGITTFGFAKQTQGGLKLDCDNAFKLPIWDNQQQLKQQCENRPTEWIKKLFEW
CCCCCCCCCCCCHHHCCCCEEEECCCCEECCCCCCHHHHHHHHHCCCHHHHHHHHCH
>Mature Secondary Structure 
TRKSSNRSRGRKARSGKSASSSKLQIWLGRIWSIGWKLALTLAAVLVFIGIYLDSMIKQ
CCCCCCHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFEGQLFDLPTVVYARILTLEPGNGLSLQELRNELDVLNYRKVAQPRFPGEYASSSSRIE
HHCCCCCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHH
LIRRPFEFADGPEPDRRVMLTFDGSGLNKIESLEQKRELGYLRLEPKLMGMLEKDSPEQR
HHHCCHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECHHHHHHHCCCCCHHH
LFLRRDQFPEVLVDALLVTEDRDFYQHDGVSPLAIGRAMVVNLKAGRTVQGGSTLTQQLA
HHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCHHHHCCEEEEEECCCCEECCCHHHHHHHH
KNIFLSSDRTLWRKLREAYMALIIDYRYSKDRILEAYLNEVYLGQSGADAIHGFGLASRL
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
YFGQPLQELRIDQLALLVGMVKGPSYYNPMRYAERARERRDLVLKLMMEHDILTAPEYQQ
HCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
AVTRPLDVQKTAQIASRQPAYFQQVSIELKEKLGDKFKADSGLRVFTSLDPVSQSKLEQA
HHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCCCHHHHHHH
IHDQIPQLAKTAGKDLEAAAIAVDRHSGEIRAMVGGKRTGYDGFNRVLNASRQIGSLVKP
HHHHHHHHHHHCCCCCCHHEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
AVYLTALAHPDQYNLATTLEDKPLTLKGSEGSAWTPRNYDRQYRGEVPLYLALAQSLNVP
HHHHHHHCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCHHHCCCCCEEHHHHHHCCCC
TVALGMKLGIDQVSATLGKLGVNRDEIRPVPSMLLGSFSLTPYQVAQMYQTLTNSGKKAP
EEEHHHHHCHHHHHHHHHHCCCCHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCH
LSALRSVLDLEGNVLYESLPRVSQAVDQQAAWLTTYAMKQGVQEGTGRYLNAQFSSAALA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHCC
GKTGTTNDNRDSWFVGVDGREVTTIWLGRDDNQPTKLTGASGALRVYAQYLKYRIPEKLQ
CCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCEECCCCHHHHHHHHHHHHCCCCCCC
LPWPEGITTFGFAKQTQGGLKLDCDNAFKLPIWDNQQQLKQQCENRPTEWIKKLFEW
CCCCCCCCCCCCHHHCCCCEEEECCCCEECCCCCCHHHHHHHHHCCCHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301