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The map label for this gene is prfA

Identifier: 147674456

GI number: 147674456

Start: 1876730

End: 1877818

Strand: Reverse

Name: prfA

Synonym: VC0395_A1756

Alternate gene names: 147674456

Gene position: 1877818-1876730 (Counterclockwise)

Preceding gene: 147674777

Following gene: 147674049

Centisome position: 62.1

GC content: 50.32

Gene sequence:

>1089_bases
ATGAAAGCGTCGATTTTAAGCAAGCTTGAATCCCTTGTTGAGCGCTATGAAGAGGTTCAGCACCTCCTTGGCGACCCAAC
AGTTATCGGTGATCAAAATAAATTCCGTGCTCTATCCAAAGAGTATTCGCAACTGGAAGAGATCACTCAGTGTTTCCAAG
CTTACCAACAAGCGAAGGAAGATCTGGTTGCTGCCGAAGAGATGGCACAAGAAGATGATGCAGAAATGCGTGAAATGGCA
CAGGACGAGATCAAAGCGGCGAAAGCGGCAATTGAGCGCCTGACCGATGAACTGCAAATTCTGTTGCTGCCAAAAGATCC
AAACGATGATCGCAACTGTTTCTTAGAAATTCGCGCGGGTGCGGGTGGTGACGAAGCGGGTATTTTTGCCGGCGATTTGT
TCCGTATGTACAGCCGTTTTGCGGAGAAAAAAGGCTGGCGCATCGAAGTGATGTCATCGAGTGAAGCGGAACACGGCGGC
TACAAAGAGATGATCGCGAAAGTGAACGGTGACGGTGCTTACGGTACGCTCAAGTTTGAATCTGGCGGCCACCGAGTGCA
ACGTGTTCCGGCTACGGAAGCGCAAGGGCGTATCCACACGTCCGCTTGTACCGTTGCGGTGATGCCTGAAATTCCTGAAG
CGGAAATTCCAGAAATCAAAGCCAGCGATCTGAAAATTGATACGTTCCGCTCTTCTGGCGCGGGTGGTCAGCACGTTAAC
ACCACCGATTCGGCTATCCGTATCACTCACTTACCGACTGGTATTGTGGTCGAGTGTCAGGATGAGCGTTCGCAACATAA
GAACAAAGCTAAAGCGATGTCAGTATTGGCTGCACGTATTGCTCAAGCTGAAGAGTCAAAACGCGCTGCGGAAATTTCCG
ATACACGCCGCAACCTTCTGGGTTCAGGCGATCGTAGTGACCGCATTCGCACTTATAACTACCCGCAAGGTCGAGTGTCC
GATCACCGTATCAATTTGACGGTATATCGCCTGACCGAAGTGATGGAAGGGGATATGCAATCCTTGATTGACCCTGTGAT
TCACGAGCATCAAGCGGATCAATTGGCGGCACTGGCGGATCAAAACTAA

Upstream 100 bases:

>100_bases
CGGCGGAACAAGGGGAACCGGCTAAACTGGCCGTGATCAGACAAAGTTTAGGTCTTGACGATCTGAACTAAATCTCAACT
TAGAGTTTAAAGAAGCAACT

Downstream 100 bases:

>100_bases
TCCATGTCAGTTACCATTGAAGCGGCATTAAAAGCGGCAACCGAGCAACTGCAGCAAAGCGGCAGTGATTCGCCAGCCCT
AGATGCCGCAGTACTCCTTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKASILSKLESLVERYEEVQHLLGDPTVIGDQNKFRALSKEYSQLEEITQCFQAYQQAKEDLVAAEEMAQEDDAEMREMA
QDEIKAAKAAIERLTDELQILLLPKDPNDDRNCFLEIRAGAGGDEAGIFAGDLFRMYSRFAEKKGWRIEVMSSSEAEHGG
YKEMIAKVNGDGAYGTLKFESGGHRVQRVPATEAQGRIHTSACTVAVMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHVN
TTDSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLAARIAQAEESKRAAEISDTRRNLLGSGDRSDRIRTYNYPQGRVS
DHRINLTVYRLTEVMEGDMQSLIDPVIHEHQADQLAALADQN

Sequences:

>Translated_362_residues
MKASILSKLESLVERYEEVQHLLGDPTVIGDQNKFRALSKEYSQLEEITQCFQAYQQAKEDLVAAEEMAQEDDAEMREMA
QDEIKAAKAAIERLTDELQILLLPKDPNDDRNCFLEIRAGAGGDEAGIFAGDLFRMYSRFAEKKGWRIEVMSSSEAEHGG
YKEMIAKVNGDGAYGTLKFESGGHRVQRVPATEAQGRIHTSACTVAVMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHVN
TTDSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLAARIAQAEESKRAAEISDTRRNLLGSGDRSDRIRTYNYPQGRVS
DHRINLTVYRLTEVMEGDMQSLIDPVIHEHQADQLAALADQN
>Mature_362_residues
MKASILSKLESLVERYEEVQHLLGDPTVIGDQNKFRALSKEYSQLEEITQCFQAYQQAKEDLVAAEEMAQEDDAEMREMA
QDEIKAAKAAIERLTDELQILLLPKDPNDDRNCFLEIRAGAGGDEAGIFAGDLFRMYSRFAEKKGWRIEVMSSSEAEHGG
YKEMIAKVNGDGAYGTLKFESGGHRVQRVPATEAQGRIHTSACTVAVMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHVN
TTDSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLAARIAQAEESKRAAEISDTRRNLLGSGDRSDRIRTYNYPQGRVS
DHRINLTVYRLTEVMEGDMQSLIDPVIHEHQADQLAALADQN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=301, Percent_Identity=42.8571428571429, Blast_Score=261, Evalue=6e-70,
Organism=Homo sapiens, GI34577120, Length=365, Percent_Identity=36.4383561643836, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=42.0289855072464, Blast_Score=181, Evalue=6e-46,
Organism=Escherichia coli, GI1787462, Length=361, Percent_Identity=72.2991689750693, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=38.622754491018, Blast_Score=218, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=284, Percent_Identity=35.2112676056338, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=307, Percent_Identity=41.3680781758958, Blast_Score=240, Evalue=2e-64,
Organism=Drosophila melanogaster, GI19921226, Length=334, Percent_Identity=39.5209580838323, Blast_Score=223, Evalue=1e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_VIBC3 (A5F696)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F696
- SMR:   A5F696
- STRING:   A5F696
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1756
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- ProtClustDB:   PRK00591
- BioCyc:   VCHO345073:VC0395_A1756-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40222; Mature: 40222

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASILSKLESLVERYEEVQHLLGDPTVIGDQNKFRALSKEYSQLEEITQCFQAYQQAKE
CCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVAAEEMAQEDDAEMREMAQDEIKAAKAAIERLTDELQILLLPKDPNDDRNCFLEIRAG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECC
AGGDEAGIFAGDLFRMYSRFAEKKGWRIEVMSSSEAEHGGYKEMIAKVNGDGAYGTLKFE
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEEC
SGGHRVQRVPATEAQGRIHTSACTVAVMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHVN
CCCCEEEECCCCCCCCCEEECEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
TTDSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLAARIAQAEESKRAAEISDTRRNLL
CCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGDRSDRIRTYNYPQGRVSDHRINLTVYRLTEVMEGDMQSLIDPVIHEHQADQLAALAD
CCCCCCCCCEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QN
CC
>Mature Secondary Structure
MKASILSKLESLVERYEEVQHLLGDPTVIGDQNKFRALSKEYSQLEEITQCFQAYQQAKE
CCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVAAEEMAQEDDAEMREMAQDEIKAAKAAIERLTDELQILLLPKDPNDDRNCFLEIRAG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECC
AGGDEAGIFAGDLFRMYSRFAEKKGWRIEVMSSSEAEHGGYKEMIAKVNGDGAYGTLKFE
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEEC
SGGHRVQRVPATEAQGRIHTSACTVAVMPEIPEAEIPEIKASDLKIDTFRSSGAGGQHVN
CCCCEEEECCCCCCCCCEEECEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
TTDSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLAARIAQAEESKRAAEISDTRRNLL
CCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGDRSDRIRTYNYPQGRVSDHRINLTVYRLTEVMEGDMQSLIDPVIHEHQADQLAALAD
CCCCCCCCCEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA