Click here to switch to the map view.

The map label for this gene is ttcA

Identifier: 147674407

GI number: 147674407

Start: 1087996

End: 1088928

Strand: Reverse

Name: ttcA

Synonym: VC0395_A1043

Alternate gene names: 147674407

Gene position: 1088928-1087996 (Counterclockwise)

Preceding gene: 147675194

Following gene: 147673678

Centisome position: 36.01

GC content: 43.62

Gene sequence:

>933_bases
ATGACAGCGCAAACTCAAGAGTTGACCAAAGCTCAACAATACAATTTCAATAAGTTGCAAAAACGTATTCGTCGCAATAC
TGGTCAAGCAATTGCAGACTTTAATATGATCGAAGATGGCGACCGGATTATGGTATGTCTATCTGGCGGTAAAGACAGCT
TTACCATGCTGGATATTTTGATGAGCCTGCAAAAAAGTGCGCCTATCTCTTTTGAATTGGTTGCTGTCAATCTCGATCAA
AAACAACCGGGATTTCCTGAGCATGTCTTGCCTGAATACCTAGATAGTTTGGGGGTTGAATACAAGATCGTAGAAGAAGA
CACCTACTCCATCGTGCAAGATAAAGTGCCTGAAGGCAAAACTACCTGCGCACTCTGCTCTCGTCTGCGTCGCGGTATTT
TATATCGTACCGCCAAAGAGTTAGGAGCAACCAAAATTGCTTTGGGTCACCATCGTGATGACATCTTAGAAACTCTGTTC
TTGAATATGTTCTACGGCGGCAAAATGAAAGGCATGCCACCTAAGCTAGTATCAGACAACGGCGAACATGTGGTTATTCG
CCCGTTGGCCTACTGCCGTGAGAAAGACATCATCAAATATTCAGATATGCGCGGCTACCCAATCATTCCATGTAACTTGT
GTGGCTCACAGCCTAACTTACAGCGCCAAGCAGTAAAACAGATGCTTAATGACTGGGATAAGCGCTTCCCTGGCCGTATT
GAAACCATGTTTAGAGCCATGCAAAACGTGGTGCCAAGCCACCTTGCAGACTTCTCTTTGTTCGATTTTAAATCGATTGA
TAAAAACTCTGGCGTCATTAATGGTGGTGATATTGGCTTTGATAAAGAAGAAATTGCACCGCAAGTCGTGGAAGATGAAG
ACTTAGTGATGGAGTTTGACCCATCACTCCAGTTAAATGTCACCAATATCTAG

Upstream 100 bases:

>100_bases
GCAGGGATTTTTTATCTCTTCACACTATAGATCTGGCATCTCTGCTATTTATCCGTATCATACGCACTTCATTTTGTTCT
GAGATTTAAGATAGCAAGCA

Downstream 100 bases:

>100_bases
GTGACTTCAAAAGCATAAAAACAAACAAAGGCTGTCTACTGACAGCCTTTGTTTTAGTCTTTCAATCGCTAAAGCTTACT
TTTTTGCAGAAGGTAAGTAA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTAQTQELTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDILMSLQKSAPISFELVAVNLDQ
KQPGFPEHVLPEYLDSLGVEYKIVEEDTYSIVQDKVPEGKTTCALCSRLRRGILYRTAKELGATKIALGHHRDDILETLF
LNMFYGGKMKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYSDMRGYPIIPCNLCGSQPNLQRQAVKQMLNDWDKRFPGRI
ETMFRAMQNVVPSHLADFSLFDFKSIDKNSGVINGGDIGFDKEEIAPQVVEDEDLVMEFDPSLQLNVTNI

Sequences:

>Translated_310_residues
MTAQTQELTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDILMSLQKSAPISFELVAVNLDQ
KQPGFPEHVLPEYLDSLGVEYKIVEEDTYSIVQDKVPEGKTTCALCSRLRRGILYRTAKELGATKIALGHHRDDILETLF
LNMFYGGKMKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYSDMRGYPIIPCNLCGSQPNLQRQAVKQMLNDWDKRFPGRI
ETMFRAMQNVVPSHLADFSLFDFKSIDKNSGVINGGDIGFDKEEIAPQVVEDEDLVMEFDPSLQLNVTNI
>Mature_309_residues
TAQTQELTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDILMSLQKSAPISFELVAVNLDQK
QPGFPEHVLPEYLDSLGVEYKIVEEDTYSIVQDKVPEGKTTCALCSRLRRGILYRTAKELGATKIALGHHRDDILETLFL
NMFYGGKMKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYSDMRGYPIIPCNLCGSQPNLQRQAVKQMLNDWDKRFPGRIE
TMFRAMQNVVPSHLADFSLFDFKSIDKNSGVINGGDIGFDKEEIAPQVVEDEDLVMEFDPSLQLNVTNI

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=312, Percent_Identity=74.3589743589744, Blast_Score=493, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI193211090, Length=250, Percent_Identity=31.6, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=237, Percent_Identity=26.5822784810127, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24651972, Length=222, Percent_Identity=28.8288288288288, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_VIBC3 (A5F888)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F888
- STRING:   A5F888
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1043
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- ProtClustDB:   PRK10696
- BioCyc:   VCHO345073:VC0395_A1043-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 35123; Mature: 34992

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTQELTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDIL
CCCCHHHHHHHHHCCHHHHHHHHHHCCCCEECCCCEECCCCEEEEEEECCCCHHHHHHHH
MSLQKSAPISFELVAVNLDQKQPGFPEHVLPEYLDSLGVEYKIVEEDTYSIVQDKVPEGK
HHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCC
TTCALCSRLRRGILYRTAKELGATKIALGHHRDDILETLFLNMFYGGKMKGMPPKLVSDN
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
GEHVVIRPLAYCREKDIIKYSDMRGYPIIPCNLCGSQPNLQRQAVKQMLNDWDKRFPGRI
CCEEEECCHHHHCCCCCEEECCCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHHCCHHH
ETMFRAMQNVVPSHLADFSLFDFKSIDKNSGVINGGDIGFDKEEIAPQVVEDEDLVMEFD
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEECCCCCCCCHHHHCCCCCCCCCEEEEEC
PSLQLNVTNI
CCEEEEECCC
>Mature Secondary Structure 
TAQTQELTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDIL
CCCHHHHHHHHHCCHHHHHHHHHHCCCCEECCCCEECCCCEEEEEEECCCCHHHHHHHH
MSLQKSAPISFELVAVNLDQKQPGFPEHVLPEYLDSLGVEYKIVEEDTYSIVQDKVPEGK
HHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCC
TTCALCSRLRRGILYRTAKELGATKIALGHHRDDILETLFLNMFYGGKMKGMPPKLVSDN
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
GEHVVIRPLAYCREKDIIKYSDMRGYPIIPCNLCGSQPNLQRQAVKQMLNDWDKRFPGRI
CCEEEECCHHHHCCCCCEEECCCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHHCCHHH
ETMFRAMQNVVPSHLADFSLFDFKSIDKNSGVINGGDIGFDKEEIAPQVVEDEDLVMEFD
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEECCCCCCCCHHHHCCCCCCCCCEEEEEC
PSLQLNVTNI
CCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA