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The map label for this gene is orfB [H]

Identifier: 147674397

GI number: 147674397

Start: 2514109

End: 2514981

Strand: Reverse

Name: orfB [H]

Synonym: VC0395_A2359

Alternate gene names: 147674397

Gene position: 2514981-2514109 (Counterclockwise)

Preceding gene: 147674790

Following gene: 147673629

Centisome position: 83.17

GC content: 44.44

Gene sequence:

>873_bases
TTGATAATCAAGAAACTCAAGCAGAGCCACAGCGTAAAAATATTATGCGAAGTCTTCAATGTTCATCGAAGTAGTTATCA
CTATTGGCTTAAACGCCCAGCGTTAATTAATGCGGAAACAATAAAACTGCGCAGCTTGATTAGCGAGGCTCACGCCGCAA
GCAATGGCTCTGCGGGAGCGAGAACCATTGCAGATATAGTCACAAATCAGGGTGTAAAGCTGAGCCGATACCGAGCAACA
AAGCTAATGAGAACTCTTGGTTTAGTAAGCTGCCAAGAACCAAAGCATCGTTACAGAAAGGCTTCACAAGAACATATTGA
CGTTCCAAATCACTTAAGTCGTCAATTTGCTGTTACGGCTCCAAATGAAGTCTGGGCTGGCGATGTTACGTATATTTGGA
CTGGTAATCGGTGGATGTATTTAGCGGTCGTTATCGATCTTTTTGCCCGCAAAGTGATCGGTTGGTCAATGTCTTTGTCG
CCTGATAGTCGGTTGACAGGTAAAGCGCTGTCTATGGCCTATGAATCTCGCGGAAAGCCAAAAGGTGTCATGTTCCATAG
CGATCAAGGCAGTCATTATACTAGCCGTAAATACCGTCAATTACTGTGGCGCTTTCAAATAAAACAGAGTTTATCTCGCC
GAGGAAACTGTTGGGATAATGCGCCGATAGAGCGCTTCTTTAGAAGTCTGAAGACGGAATGGGTGCCAACTGTGGGTTAT
CGTAGCTTCGCTGAGGCTCAACAAGAGATCACCCGCTACATTATCGGATATTACTGCCAACTCAGGCCACATCAGTATAA
CGGTGGTCTAACTCCCAATGAATCAGAACGATTATATTGGGAAAACTCTAAAACCGTGGCCAATTTTAGTTGA

Upstream 100 bases:

>100_bases
AAATTCGGGAATTGAAAAAGAAGCTGGCTCGCCTTGAAGAGCATAATGAAATACTAAAAAAAGCCACGGCTCTCTTGATG
TCGGACTCACTGAACAATTC

Downstream 100 bases:

>100_bases
CCACTACAAATCTTGCTGAGCGATTCACAAATAAAAAGTGGAAGAACAAGCTTTATCTTTATCCCATCGATGTCCGTTCA
GCAAGAGGCTCTCGATTTGT

Product: transposase OrfAB, subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MIIKKLKQSHSVKILCEVFNVHRSSYHYWLKRPALINAETIKLRSLISEAHAASNGSAGARTIADIVTNQGVKLSRYRAT
KLMRTLGLVSCQEPKHRYRKASQEHIDVPNHLSRQFAVTAPNEVWAGDVTYIWTGNRWMYLAVVIDLFARKVIGWSMSLS
PDSRLTGKALSMAYESRGKPKGVMFHSDQGSHYTSRKYRQLLWRFQIKQSLSRRGNCWDNAPIERFFRSLKTEWVPTVGY
RSFAEAQQEITRYIIGYYCQLRPHQYNGGLTPNESERLYWENSKTVANFS

Sequences:

>Translated_290_residues
MIIKKLKQSHSVKILCEVFNVHRSSYHYWLKRPALINAETIKLRSLISEAHAASNGSAGARTIADIVTNQGVKLSRYRAT
KLMRTLGLVSCQEPKHRYRKASQEHIDVPNHLSRQFAVTAPNEVWAGDVTYIWTGNRWMYLAVVIDLFARKVIGWSMSLS
PDSRLTGKALSMAYESRGKPKGVMFHSDQGSHYTSRKYRQLLWRFQIKQSLSRRGNCWDNAPIERFFRSLKTEWVPTVGY
RSFAEAQQEITRYIIGYYCQLRPHQYNGGLTPNESERLYWENSKTVANFS
>Mature_290_residues
MIIKKLKQSHSVKILCEVFNVHRSSYHYWLKRPALINAETIKLRSLISEAHAASNGSAGARTIADIVTNQGVKLSRYRAT
KLMRTLGLVSCQEPKHRYRKASQEHIDVPNHLSRQFAVTAPNEVWAGDVTYIWTGNRWMYLAVVIDLFARKVIGWSMSLS
PDSRLTGKALSMAYESRGKPKGVMFHSDQGSHYTSRKYRQLLWRFQIKQSLSRRGNCWDNAPIERFFRSLKTEWVPTVGY
RSFAEAQQEITRYIIGYYCQLRPHQYNGGLTPNESERLYWENSKTVANFS

Specific function: Involved in the transposition of the insertion sequence IS911B [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=241, Percent_Identity=30.2904564315353, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1788405, Length=282, Percent_Identity=33.6879432624113, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787263, Length=282, Percent_Identity=33.6879432624113, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1786753, Length=282, Percent_Identity=33.6879432624113, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1786570, Length=282, Percent_Identity=33.6879432624113, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1786490, Length=282, Percent_Identity=33.6879432624113, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1789981, Length=275, Percent_Identity=34.1818181818182, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33555; Mature: 33555

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKKLKQSHSVKILCEVFNVHRSSYHYWLKRPALINAETIKLRSLISEAHAASNGSAGA
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHCCCCCCHH
RTIADIVTNQGVKLSRYRATKLMRTLGLVSCQEPKHRYRKASQEHIDVPNHLSRQFAVTA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHCCCCHHHCCCEEEEC
PNEVWAGDVTYIWTGNRWMYLAVVIDLFARKVIGWSMSLSPDSRLTGKALSMAYESRGKP
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
KGVMFHSDQGSHYTSRKYRQLLWRFQIKQSLSRRGNCWDNAPIERFFRSLKTEWVPTVGY
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCH
RSFAEAQQEITRYIIGYYCQLRPHQYNGGLTPNESERLYWENSKTVANFS
HHHHHHHHHHHHHHHHHHHEECCEECCCCCCCCCCCEEEECCCCEEECCC
>Mature Secondary Structure
MIIKKLKQSHSVKILCEVFNVHRSSYHYWLKRPALINAETIKLRSLISEAHAASNGSAGA
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHCCCCCCHH
RTIADIVTNQGVKLSRYRATKLMRTLGLVSCQEPKHRYRKASQEHIDVPNHLSRQFAVTA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHCCCCHHHCCCEEEEC
PNEVWAGDVTYIWTGNRWMYLAVVIDLFARKVIGWSMSLSPDSRLTGKALSMAYESRGKP
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
KGVMFHSDQGSHYTSRKYRQLLWRFQIKQSLSRRGNCWDNAPIERFFRSLKTEWVPTVGY
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCH
RSFAEAQQEITRYIIGYYCQLRPHQYNGGLTPNESERLYWENSKTVANFS
HHHHHHHHHHHHHHHHHHHEECCEECCCCCCCCCCCEEEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]