Click here to switch to the map view.

The map label for this gene is dusC [H]

Identifier: 147674360

GI number: 147674360

Start: 666435

End: 666911

Strand: Reverse

Name: dusC [H]

Synonym: VC0395_A0624

Alternate gene names: 147674360

Gene position: 666911-666435 (Counterclockwise)

Preceding gene: 147674377

Following gene: 147674229

Centisome position: 22.05

GC content: 50.94

Gene sequence:

>477_bases
ATGCGAGTTATTTTAGGCCCGATGGAGGGCGTGTTAGATCATTTGATGCGTGACATGCTGACACAAATCAATGACTACGA
CTTCTGCGTCACCGAATTTGTGCGCGTAGTGAATCTGCTGCTGCCTGATCATGTGTTTTATCGCCTCTGTCCTGAATTAC
GGCACGGTTCTAAAACTCCGTCCGGTGTTCCGGTAAAAGTACAACTGCTTGGCCAAGACCCACACTGGATGGCCGAAAAC
GCGATCCGCGCTGCTGAGCTTGGGGCTTATGGCATCGATCTAAATTTCGGCTGCCCTGCCAAAATGGTCAATCAGAGCAA
AGGTGGCGCGGCTCTTTTGCAGCATCCCGAGTTGATTTATCAGGTGGTCAAAGCGTGTCGTGACGCAGTACCGGCGCACA
TTCCTGTTTCGGCGAAAATCCGCTTAGGTTGGGAAGATCCGGAAGATTGTTTTGAGATTGTCGATGCCGTTGCGTAA

Upstream 100 bases:

>100_bases
TGTCACACTGAACTTGAATCAGAAAGGATGGGTGACATTTATTGATGCTACTACTACAATCTCGCTCCCTTTTCTTCAGC
GCTAGCCGGTATTCATCACT

Downstream 100 bases:

>100_bases
GCTAAAGCCGATGAGCTCACTGTGCACGCGCGCACCAAAGCCGGAGGCTACAAGGCCAGCGAAATCAAATGGCATTACAT
TGACCAGATCCGCCAAAAAT

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MRVILGPMEGVLDHLMRDMLTQINDYDFCVTEFVRVVNLLLPDHVFYRLCPELRHGSKTPSGVPVKVQLLGQDPHWMAEN
AIRAAELGAYGIDLNFGCPAKMVNQSKGGAALLQHPELIYQVVKACRDAVPAHIPVSAKIRLGWEDPEDCFEIVDAVA

Sequences:

>Translated_158_residues
MRVILGPMEGVLDHLMRDMLTQINDYDFCVTEFVRVVNLLLPDHVFYRLCPELRHGSKTPSGVPVKVQLLGQDPHWMAEN
AIRAAELGAYGIDLNFGCPAKMVNQSKGGAALLQHPELIYQVVKACRDAVPAHIPVSAKIRLGWEDPEDCFEIVDAVA
>Mature_158_residues
MRVILGPMEGVLDHLMRDMLTQINDYDFCVTEFVRVVNLLLPDHVFYRLCPELRHGSKTPSGVPVKVQLLGQDPHWMAEN
AIRAAELGAYGIDLNFGCPAKMVNQSKGGAALLQHPELIYQVVKACRDAVPAHIPVSAKIRLGWEDPEDCFEIVDAVA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788462, Length=157, Percent_Identity=66.8789808917197, Blast_Score=228, Evalue=2e-61,
Organism=Escherichia coli, GI1789660, Length=154, Percent_Identity=33.7662337662338, Blast_Score=73, Evalue=7e-15,
Organism=Escherichia coli, GI145693211, Length=91, Percent_Identity=40.6593406593407, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 17548; Mature: 17548

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILGPMEGVLDHLMRDMLTQINDYDFCVTEFVRVVNLLLPDHVFYRLCPELRHGSKTP
CCEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHCHHHHCCCCCC
SGVPVKVQLLGQDPHWMAENAIRAAELGAYGIDLNFGCPAKMVNQSKGGAALLQHPELIY
CCCCEEEEEECCCCCHHHHHHHHHHHHCCEEECCCCCCCHHHHCCCCCCHHHHCCHHHHH
QVVKACRDAVPAHIPVSAKIRLGWEDPEDCFEIVDAVA
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRVILGPMEGVLDHLMRDMLTQINDYDFCVTEFVRVVNLLLPDHVFYRLCPELRHGSKTP
CCEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHCHHHHCCCCCC
SGVPVKVQLLGQDPHWMAENAIRAAELGAYGIDLNFGCPAKMVNQSKGGAALLQHPELIY
CCCCEEEEEECCCCCHHHHHHHHHHHHCCEEECCCCCCCHHHHCCCCCCHHHHCCHHHHH
QVVKACRDAVPAHIPVSAKIRLGWEDPEDCFEIVDAVA
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]