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The map label for this gene is ywbI [H]

Identifier: 147674340

GI number: 147674340

Start: 1284129

End: 1284998

Strand: Reverse

Name: ywbI [H]

Synonym: VC0395_A1224

Alternate gene names: 147674340

Gene position: 1284998-1284129 (Counterclockwise)

Preceding gene: 147675379

Following gene: 147673336

Centisome position: 42.49

GC content: 45.29

Gene sequence:

>870_bases
ATGGAAGTTCGACAACTGCGTCACTTTGTGACTGTGGCTGAATGGGAAAGTTTTACCTTAGCCGCGCAGCAACTACATAT
TGCGCAGCCTGCACTGAGTATTTCGATTAAAAAATTTGAGCAGCAGCTCGGTGTTACTCTGTTTAAAAGGGATGAAAGAA
AAGTTGCGCTGACCCATGAAGGTGCAGTGTTGCTTGAACATGCCAAGCGCATTTTGCAACAAGTCAATGATGCCCAATTG
GCGGTTGATGAGCTCAAAGGTGTTGTGAAAGGCGAGGTGCGTCTTGGCGCGCCAAGCATGATGGGCTCTTACTTTTTCCC
GCAAATCATTATGGCGTTTAAAAGCCACTTTCCGAATCTGAAAATGACGGTGATTGAAGCGGGTACTCAATCGATCCGTA
CAATGCTGCTCTCAGGCGAGTTGGATATTGGGGTGATTTTGAATCACGATCTGCCGGACGATTTGGCTGCAGATCCGCTG
CTCTCTTCGCAAATGGTTGCTGTAGTCGGCAAAAATCATGAACTAGCTGAACGTCGGCATCTTACTTTTGCCGAGTTTTT
TGAGCATGAACTGGTAATGTTTAAACCCGGCTATTTTCACCGAGATTTTATCGATAAAGTGTGCCGAGAACATAAGTTTA
CTGCAGATTTTTCTTTTGAAACCAATTTGTTACCTATGATTTTAAGCATAGTGAAACATGAATATGCGATTACCGCGCTG
CTGGAGTTGGTGTCCAGCAATGAACCGGAAGTGAAGGCGATTCCGTTTGAACCGCCGGTGTGGCTTGATCTTGCATTGGC
TTGGCGTAAAGAAGGTTATCTGTCCAATGCCGACCGTACTTTCATCGAGTTTGTGAAAAAGTACGTTTAG

Upstream 100 bases:

>100_bases
GGATCCAAGGTGCGAAAAGGCGGTGATGTGATCACGATAGCAGTGGAATCTCGATTAATGAAATATATTAAAAATATGGT
TGTGATATACAGGATATATA

Downstream 100 bases:

>100_bases
CGCCAGAAGAGCATTCATCTTTGCTGCTATTAATCTTTACTGCTGTAGATACGTTCAAAATTGGTGTGGTTCCAGCCGAT
CATCAAACGATCACCAATTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MEVRQLRHFVTVAEWESFTLAAQQLHIAQPALSISIKKFEQQLGVTLFKRDERKVALTHEGAVLLEHAKRILQQVNDAQL
AVDELKGVVKGEVRLGAPSMMGSYFFPQIIMAFKSHFPNLKMTVIEAGTQSIRTMLLSGELDIGVILNHDLPDDLAADPL
LSSQMVAVVGKNHELAERRHLTFAEFFEHELVMFKPGYFHRDFIDKVCREHKFTADFSFETNLLPMILSIVKHEYAITAL
LELVSSNEPEVKAIPFEPPVWLDLALAWRKEGYLSNADRTFIEFVKKYV

Sequences:

>Translated_289_residues
MEVRQLRHFVTVAEWESFTLAAQQLHIAQPALSISIKKFEQQLGVTLFKRDERKVALTHEGAVLLEHAKRILQQVNDAQL
AVDELKGVVKGEVRLGAPSMMGSYFFPQIIMAFKSHFPNLKMTVIEAGTQSIRTMLLSGELDIGVILNHDLPDDLAADPL
LSSQMVAVVGKNHELAERRHLTFAEFFEHELVMFKPGYFHRDFIDKVCREHKFTADFSFETNLLPMILSIVKHEYAITAL
LELVSSNEPEVKAIPFEPPVWLDLALAWRKEGYLSNADRTFIEFVKKYV
>Mature_289_residues
MEVRQLRHFVTVAEWESFTLAAQQLHIAQPALSISIKKFEQQLGVTLFKRDERKVALTHEGAVLLEHAKRILQQVNDAQL
AVDELKGVVKGEVRLGAPSMMGSYFFPQIIMAFKSHFPNLKMTVIEAGTQSIRTMLLSGELDIGVILNHDLPDDLAADPL
LSSQMVAVVGKNHELAERRHLTFAEFFEHELVMFKPGYFHRDFIDKVCREHKFTADFSFETNLLPMILSIVKHEYAITAL
LELVSSNEPEVKAIPFEPPVWLDLALAWRKEGYLSNADRTFIEFVKKYV

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=283, Percent_Identity=29.3286219081272, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI145693105, Length=283, Percent_Identity=28.9752650176678, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1790399, Length=260, Percent_Identity=28.4615384615385, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787879, Length=290, Percent_Identity=30, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI2367136, Length=283, Percent_Identity=26.5017667844523, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788887, Length=294, Percent_Identity=28.9115646258503, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788297, Length=162, Percent_Identity=33.9506172839506, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788481, Length=289, Percent_Identity=23.8754325259516, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788748, Length=288, Percent_Identity=25, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787601, Length=154, Percent_Identity=31.1688311688312, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788296, Length=204, Percent_Identity=27.9411764705882, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082132, Length=294, Percent_Identity=25.5102040816327, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081904, Length=213, Percent_Identity=28.169014084507, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789204, Length=248, Percent_Identity=23.7903225806452, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786401, Length=299, Percent_Identity=24.4147157190635, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787530, Length=235, Percent_Identity=22.1276595744681, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789506, Length=158, Percent_Identity=32.2784810126582, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787589, Length=194, Percent_Identity=28.3505154639175, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789639, Length=125, Percent_Identity=25.6, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790262, Length=195, Percent_Identity=25.6410256410256, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787806, Length=160, Percent_Identity=26.875, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788706, Length=183, Percent_Identity=27.8688524590164, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790208, Length=145, Percent_Identity=26.2068965517241, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787949, Length=136, Percent_Identity=23.5294117647059, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787128, Length=158, Percent_Identity=27.2151898734177, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32961; Mature: 32961

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVRQLRHFVTVAEWESFTLAAQQLHIAQPALSISIKKFEQQLGVTLFKRDERKVALTHE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEECCCCEEEEEEC
GAVLLEHAKRILQQVNDAQLAVDELKGVVKGEVRLGAPSMMGSYFFPQIIMAFKSHFPNL
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHCCCC
KMTVIEAGTQSIRTMLLSGELDIGVILNHDLPDDLAADPLLSSQMVAVVGKNHELAERRH
EEEEEHHCHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCCCCCCCEEECCCCHHHHHHC
LTFAEFFEHELVMFKPGYFHRDFIDKVCREHKFTADFSFETNLLPMILSIVKHEYAITAL
CHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LELVSSNEPEVKAIPFEPPVWLDLALAWRKEGYLSNADRTFIEFVKKYV
HHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MEVRQLRHFVTVAEWESFTLAAQQLHIAQPALSISIKKFEQQLGVTLFKRDERKVALTHE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEECCCCEEEEEEC
GAVLLEHAKRILQQVNDAQLAVDELKGVVKGEVRLGAPSMMGSYFFPQIIMAFKSHFPNL
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHCCCC
KMTVIEAGTQSIRTMLLSGELDIGVILNHDLPDDLAADPLLSSQMVAVVGKNHELAERRH
EEEEEHHCHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCCCCCCCEEECCCCHHHHHHC
LTFAEFFEHELVMFKPGYFHRDFIDKVCREHKFTADFSFETNLLPMILSIVKHEYAITAL
CHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LELVSSNEPEVKAIPFEPPVWLDLALAWRKEGYLSNADRTFIEFVKKYV
HHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]